AU2013276467B2 - CHO expression system - Google Patents
CHO expression system Download PDFInfo
- Publication number
- AU2013276467B2 AU2013276467B2 AU2013276467A AU2013276467A AU2013276467B2 AU 2013276467 B2 AU2013276467 B2 AU 2013276467B2 AU 2013276467 A AU2013276467 A AU 2013276467A AU 2013276467 A AU2013276467 A AU 2013276467A AU 2013276467 B2 AU2013276467 B2 AU 2013276467B2
- Authority
- AU
- Australia
- Prior art keywords
- gly
- ala
- glu
- arg
- ile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
- C12N2820/55—Vectors comprising a special origin of replication system from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12305677.2 | 2012-06-14 | ||
| EP12305677.2A EP2674495A1 (en) | 2012-06-14 | 2012-06-14 | CHO expression system |
| PCT/EP2013/062400 WO2013186371A1 (en) | 2012-06-14 | 2013-06-14 | Cho expression system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2013276467A1 AU2013276467A1 (en) | 2015-01-15 |
| AU2013276467B2 true AU2013276467B2 (en) | 2018-08-30 |
Family
ID=48700539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2013276467A Active AU2013276467B2 (en) | 2012-06-14 | 2013-06-14 | CHO expression system |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9334489B2 (enExample) |
| EP (2) | EP2674495A1 (enExample) |
| CN (2) | CN104540950A (enExample) |
| AR (1) | AR091418A1 (enExample) |
| AU (1) | AU2013276467B2 (enExample) |
| CA (1) | CA2876550C (enExample) |
| DK (1) | DK2861741T3 (enExample) |
| ES (1) | ES2627954T3 (enExample) |
| HU (1) | HUE032968T2 (enExample) |
| IN (1) | IN2014KN02823A (enExample) |
| MX (1) | MX353499B (enExample) |
| SG (1) | SG11201408051VA (enExample) |
| TW (1) | TWI605123B (enExample) |
| UY (1) | UY34860A (enExample) |
| WO (1) | WO2013186371A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR102333A1 (es) | 2014-08-19 | 2017-02-22 | Regeneron Pharma | Selectividad eficiente de proteínas recombinadas |
| SG11201702516UA (en) * | 2014-10-23 | 2017-04-27 | Sanofi Sa | Novel selection marker for cell transfection and protein production |
| US11098310B2 (en) | 2016-01-27 | 2021-08-24 | Just-Evotec Biologics, Inc. | Expression from transposon-based vectors and uses |
| US11261462B2 (en) | 2016-01-27 | 2022-03-01 | Just-Evotec Biologics, Inc. | Inducible expression from transposon-based vectors and uses |
| ES2823173T3 (es) | 2016-01-27 | 2021-05-06 | Just Biotherapeutics Inc | Promotor híbrido y usos del mismo |
| WO2018047154A1 (en) | 2016-09-07 | 2018-03-15 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Anti-nkp46 antibodies and therapeutic use of same |
| CN111093641A (zh) | 2017-05-24 | 2020-05-01 | 托埃瑞斯有限责任公司 | 谷氨酰胺合成酶用于治疗高氨血症的用途 |
| IL253984A0 (en) | 2017-08-14 | 2017-09-28 | Yeda Res & Dev | Immunotherapy against aranviruses that bind through transferrin receptor 1 (tfr1 |
| CN108085337B (zh) * | 2017-12-28 | 2020-10-16 | 未名生物医药有限公司 | 一种gs表达系统细胞株的筛选方法 |
| CN109988777A (zh) * | 2017-12-29 | 2019-07-09 | 南京金斯瑞生物科技有限公司 | 谷氨酰胺合成酶基因以及应用 |
| US20220356487A1 (en) * | 2019-06-07 | 2022-11-10 | Biocon Biologics India Limited | Mammalian expression vectors |
| IL276627A (en) | 2020-08-10 | 2022-03-01 | Yeda Res & Dev | Compositions for diagnosis and treatment of coronavirus infections |
| IL301442A (en) | 2020-09-21 | 2023-05-01 | Sanofi Sa | Production of a recombinant Chinese hamster ovary cell line (CHO) for the production of a high-yield therapeutic protein |
| EP4384623A4 (en) * | 2021-08-03 | 2025-09-24 | Shanghai Zhenge Biotechnology Co Ltd | SELECTABLE MARKERS FOR EUKARYOTIC EXPRESSION SYSTEM |
| CN118804978A (zh) * | 2021-12-27 | 2024-10-18 | 上海臻格生物技术有限公司 | 用于重组生产系统的新型标志物 |
| CN115287300A (zh) * | 2022-08-11 | 2022-11-04 | 无锡多宁生物科技有限公司 | 一种增强ExpiCHO细胞瞬时转染抗体表达的方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8601597D0 (en) | 1986-01-23 | 1986-02-26 | Wilson R H | Nucleotide sequences |
| GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
| GB8924021D0 (en) * | 1989-10-25 | 1989-12-13 | Celltech Ltd | Recombinant dna method and vectors for the use therein |
| KR100267720B1 (ko) * | 1997-12-29 | 2001-03-02 | 박호군 | 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법 |
| GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
| JP4764818B2 (ja) * | 2003-04-11 | 2011-09-07 | メディミューン，エルエルシー | 組換えｉｌ−９抗体およびその使用 |
| US7244616B2 (en) * | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| EP2298890A1 (en) * | 2004-12-30 | 2011-03-23 | Agency for Science, Technology and Research | Chinese hamster apoptosis-related genes |
| GB0508154D0 (en) * | 2005-04-22 | 2005-06-01 | Lonza Biologics Plc | Mammalian expression vector comprising the mCMV promoter and first intron of hCMV major immediate early gene |
| GB0510277D0 (en) * | 2005-05-20 | 2005-06-29 | Lonza Biologics Plc | High-level expression of recombinant antibody in a mammalian host cell |
| EP2109674A2 (en) * | 2007-01-08 | 2009-10-21 | Millipore Corporation | High expression cell line that eliminates gene amplification |
| LT2592148T (lt) * | 2007-10-12 | 2018-12-10 | F. Hoffmann-La Roche Ag | Baltymų ekspresavimas iš kelių nukleorūgščių |
| MY158903A (en) | 2007-11-16 | 2016-11-30 | Univ Rockefeller | Antibodies specific for the protofibril form of beta-amyloid protein |
-
2012
- 2012-06-14 EP EP12305677.2A patent/EP2674495A1/en not_active Withdrawn
-
2013
- 2013-06-12 AR ARP130102062 patent/AR091418A1/es unknown
- 2013-06-14 IN IN2823KON2014 patent/IN2014KN02823A/en unknown
- 2013-06-14 MX MX2014015280A patent/MX353499B/es active IP Right Grant
- 2013-06-14 WO PCT/EP2013/062400 patent/WO2013186371A1/en not_active Ceased
- 2013-06-14 TW TW102121230A patent/TWI605123B/zh active
- 2013-06-14 CN CN201380042643.6A patent/CN104540950A/zh active Pending
- 2013-06-14 HU HUE13732112A patent/HUE032968T2/en unknown
- 2013-06-14 AU AU2013276467A patent/AU2013276467B2/en active Active
- 2013-06-14 SG SG11201408051VA patent/SG11201408051VA/en unknown
- 2013-06-14 UY UY0001034860A patent/UY34860A/es not_active Application Discontinuation
- 2013-06-14 CA CA2876550A patent/CA2876550C/en active Active
- 2013-06-14 CN CN201711180397.7A patent/CN107881151A/zh active Pending
- 2013-06-14 DK DK13732112.1T patent/DK2861741T3/en active
- 2013-06-14 EP EP13732112.1A patent/EP2861741B1/en active Active
- 2013-06-14 ES ES13732112.1T patent/ES2627954T3/es active Active
- 2013-06-14 US US14/407,836 patent/US9334489B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| SG11201408051VA (en) | 2015-01-29 |
| TWI605123B (zh) | 2017-11-11 |
| TW201402817A (zh) | 2014-01-16 |
| WO2013186371A1 (en) | 2013-12-19 |
| CA2876550C (en) | 2021-07-13 |
| MX2014015280A (es) | 2015-06-23 |
| DK2861741T3 (en) | 2017-07-17 |
| AU2013276467A1 (en) | 2015-01-15 |
| US9334489B2 (en) | 2016-05-10 |
| CA2876550A1 (en) | 2013-12-19 |
| EP2674495A1 (en) | 2013-12-18 |
| MX353499B (es) | 2018-01-16 |
| EP2861741A1 (en) | 2015-04-22 |
| AR091418A1 (es) | 2015-02-04 |
| UY34860A (es) | 2014-01-31 |
| HUE032968T2 (en) | 2017-11-28 |
| US20150140607A1 (en) | 2015-05-21 |
| CN104540950A (zh) | 2015-04-22 |
| EP2861741B1 (en) | 2017-04-12 |
| IN2014KN02823A (enExample) | 2015-05-08 |
| CN107881151A (zh) | 2018-04-06 |
| ES2627954T3 (es) | 2017-08-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |