CN104540950A - Cho表达系统 - Google Patents

Cho表达系统 Download PDF

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Publication number
CN104540950A
CN104540950A CN201380042643.6A CN201380042643A CN104540950A CN 104540950 A CN104540950 A CN 104540950A CN 201380042643 A CN201380042643 A CN 201380042643A CN 104540950 A CN104540950 A CN 104540950A
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CN
China
Prior art keywords
sequence
cell line
seq
vector
cho cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201380042643.6A
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English (en)
Chinese (zh)
Inventor
C·德沃德
B·杜马斯
N·劳尼斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Aventis France
Original Assignee
Sanofi Aventis France
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Aventis France filed Critical Sanofi Aventis France
Priority to CN201711180397.7A priority Critical patent/CN107881151A/zh
Publication of CN104540950A publication Critical patent/CN104540950A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2820/00—Vectors comprising a special origin of replication system
    • C12N2820/55—Vectors comprising a special origin of replication system from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201380042643.6A 2012-06-14 2013-06-14 Cho表达系统 Pending CN104540950A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711180397.7A CN107881151A (zh) 2012-06-14 2013-06-14 Cho表达系统

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12305677.2 2012-06-14
EP12305677.2A EP2674495A1 (en) 2012-06-14 2012-06-14 CHO expression system
PCT/EP2013/062400 WO2013186371A1 (en) 2012-06-14 2013-06-14 Cho expression system

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201711180397.7A Division CN107881151A (zh) 2012-06-14 2013-06-14 Cho表达系统

Publications (1)

Publication Number Publication Date
CN104540950A true CN104540950A (zh) 2015-04-22

Family

ID=48700539

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201380042643.6A Pending CN104540950A (zh) 2012-06-14 2013-06-14 Cho表达系统
CN201711180397.7A Pending CN107881151A (zh) 2012-06-14 2013-06-14 Cho表达系统

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201711180397.7A Pending CN107881151A (zh) 2012-06-14 2013-06-14 Cho表达系统

Country Status (15)

Country Link
US (1) US9334489B2 (enExample)
EP (2) EP2674495A1 (enExample)
CN (2) CN104540950A (enExample)
AR (1) AR091418A1 (enExample)
AU (1) AU2013276467B2 (enExample)
CA (1) CA2876550C (enExample)
DK (1) DK2861741T3 (enExample)
ES (1) ES2627954T3 (enExample)
HU (1) HUE032968T2 (enExample)
IN (1) IN2014KN02823A (enExample)
MX (1) MX353499B (enExample)
SG (1) SG11201408051VA (enExample)
TW (1) TWI605123B (enExample)
UY (1) UY34860A (enExample)
WO (1) WO2013186371A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108085337A (zh) * 2017-12-28 2018-05-29 未名生物医药有限公司 一种gs表达系统细胞株的筛选方法
WO2019129215A1 (zh) * 2017-12-29 2019-07-04 南京金斯瑞生物科技有限公司 谷氨酰胺合成酶基因以及应用
CN115287300A (zh) * 2022-08-11 2022-11-04 无锡多宁生物科技有限公司 一种增强ExpiCHO细胞瞬时转染抗体表达的方法
WO2023125389A1 (en) * 2021-12-27 2023-07-06 Shanghai Zhenge Biotechnology Co., Ltd. Novel markers for recombinant production system

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
SG11201702516UA (en) * 2014-10-23 2017-04-27 Sanofi Sa Novel selection marker for cell transfection and protein production
US11098310B2 (en) 2016-01-27 2021-08-24 Just-Evotec Biologics, Inc. Expression from transposon-based vectors and uses
US11261462B2 (en) 2016-01-27 2022-03-01 Just-Evotec Biologics, Inc. Inducible expression from transposon-based vectors and uses
ES2823173T3 (es) 2016-01-27 2021-05-06 Just Biotherapeutics Inc Promotor híbrido y usos del mismo
WO2018047154A1 (en) 2016-09-07 2018-03-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Anti-nkp46 antibodies and therapeutic use of same
CN111093641A (zh) 2017-05-24 2020-05-01 托埃瑞斯有限责任公司 谷氨酰胺合成酶用于治疗高氨血症的用途
IL253984A0 (en) 2017-08-14 2017-09-28 Yeda Res & Dev Immunotherapy against aranviruses that bind through transferrin receptor 1 (tfr1
US20220356487A1 (en) * 2019-06-07 2022-11-10 Biocon Biologics India Limited Mammalian expression vectors
IL276627A (en) 2020-08-10 2022-03-01 Yeda Res & Dev Compositions for diagnosis and treatment of coronavirus infections
IL301442A (en) 2020-09-21 2023-05-01 Sanofi Sa Production of a recombinant Chinese hamster ovary cell line (CHO) for the production of a high-yield therapeutic protein
EP4384623A4 (en) * 2021-08-03 2025-09-24 Shanghai Zhenge Biotechnology Co Ltd SELECTABLE MARKERS FOR EUKARYOTIC EXPRESSION SYSTEM

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006657A1 (en) * 1989-10-25 1991-05-16 Celltech Limited Recombinant dna method and vectors for use therein
KR100267720B1 (ko) * 1997-12-29 2001-03-02 박호군 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법

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GB8601597D0 (en) 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences
GB8717430D0 (en) 1987-07-23 1987-08-26 Celltech Ltd Recombinant dna product
GB0216648D0 (en) * 2002-07-18 2002-08-28 Lonza Biologics Plc Method of expressing recombinant protein in CHO cells
JP4764818B2 (ja) * 2003-04-11 2011-09-07 メディミューン，エルエルシー 組換えｉｌ−９抗体およびその使用
US7244616B2 (en) * 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
EP2298890A1 (en) * 2004-12-30 2011-03-23 Agency for Science, Technology and Research Chinese hamster apoptosis-related genes
GB0508154D0 (en) * 2005-04-22 2005-06-01 Lonza Biologics Plc Mammalian expression vector comprising the mCMV promoter and first intron of hCMV major immediate early gene
GB0510277D0 (en) * 2005-05-20 2005-06-29 Lonza Biologics Plc High-level expression of recombinant antibody in a mammalian host cell
EP2109674A2 (en) * 2007-01-08 2009-10-21 Millipore Corporation High expression cell line that eliminates gene amplification
LT2592148T (lt) * 2007-10-12 2018-12-10 F. Hoffmann-La Roche Ag Baltymų ekspresavimas iš kelių nukleorūgščių
MY158903A (en) 2007-11-16 2016-11-30 Univ Rockefeller Antibodies specific for the protofibril form of beta-amyloid protein

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006657A1 (en) * 1989-10-25 1991-05-16 Celltech Limited Recombinant dna method and vectors for use therein
KR100267720B1 (ko) * 1997-12-29 2001-03-02 박호군 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법

Non-Patent Citations (3)

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Title
KUO C F等: "Mouse glutamine synthetase is encoded by a single gene that can be expressed in a localized fashion.", 《JOURNAL OF MOLECULAR BIOLOGY》, vol. 208, no. 1, 5 July 1989 (1989-07-05), pages 45 - 56, XP024019156, DOI: doi:10.1016/0022-2836(89)90086-7 *
OMASA T等: "Expression and amplification of glutamine synthetase gene endows HepG2 cells with ammonia-metabolizing activity for bioartificial liver support system.", 《ENZYME AND MICROBIAL TECHNOLOGY》, vol. 35, no. 67, 1 December 2004 (2004-12-01), pages 519 - 524, XP004619945, DOI: doi:10.1016/j.enzmictec.2004.08.027 *
PU H等: "RAPID ESTABLISHMENT OF HIGH-PRODUCING CELL LINES USING DICISTRONIC VECTORS WITH GLUTAMINE SYNTHETASE AS THE SELECTION MARKER.", 《MOLECULAR BIOTECHNOLOGY》, vol. 10, no. 1, 1 August 1998 (1998-08-01), pages 17 - 25, XP009021044, DOI: doi:10.1007/BF02745860 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108085337A (zh) * 2017-12-28 2018-05-29 未名生物医药有限公司 一种gs表达系统细胞株的筛选方法
CN108085337B (zh) * 2017-12-28 2020-10-16 未名生物医药有限公司 一种gs表达系统细胞株的筛选方法
WO2019129215A1 (zh) * 2017-12-29 2019-07-04 南京金斯瑞生物科技有限公司 谷氨酰胺合成酶基因以及应用
CN109988777A (zh) * 2017-12-29 2019-07-09 南京金斯瑞生物科技有限公司 谷氨酰胺合成酶基因以及应用
WO2023125389A1 (en) * 2021-12-27 2023-07-06 Shanghai Zhenge Biotechnology Co., Ltd. Novel markers for recombinant production system
CN115287300A (zh) * 2022-08-11 2022-11-04 无锡多宁生物科技有限公司 一种增强ExpiCHO细胞瞬时转染抗体表达的方法

Also Published As

Publication number Publication date
SG11201408051VA (en) 2015-01-29
TWI605123B (zh) 2017-11-11
TW201402817A (zh) 2014-01-16
WO2013186371A1 (en) 2013-12-19
CA2876550C (en) 2021-07-13
MX2014015280A (es) 2015-06-23
DK2861741T3 (en) 2017-07-17
AU2013276467A1 (en) 2015-01-15
US9334489B2 (en) 2016-05-10
CA2876550A1 (en) 2013-12-19
EP2674495A1 (en) 2013-12-18
MX353499B (es) 2018-01-16
EP2861741A1 (en) 2015-04-22
AR091418A1 (es) 2015-02-04
UY34860A (es) 2014-01-31
HUE032968T2 (en) 2017-11-28
US20150140607A1 (en) 2015-05-21
EP2861741B1 (en) 2017-04-12
AU2013276467B2 (en) 2018-08-30
IN2014KN02823A (enExample) 2015-05-08
CN107881151A (zh) 2018-04-06
ES2627954T3 (es) 2017-08-01

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