AU2010276236B2 - Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range - Google Patents

Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range Download PDF

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Publication number
AU2010276236B2
AU2010276236B2 AU2010276236A AU2010276236A AU2010276236B2 AU 2010276236 B2 AU2010276236 B2 AU 2010276236B2 AU 2010276236 A AU2010276236 A AU 2010276236A AU 2010276236 A AU2010276236 A AU 2010276236A AU 2010276236 B2 AU2010276236 B2 AU 2010276236B2
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Australia
Prior art keywords
amplification
amplicon
probes
nucleic acid
amplicons
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AU2010276236A
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AU2010276236A1 (en
Inventor
Kenneth A. Browne
Daniel L. Kacian
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Gen Probe Inc
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Gen Probe Inc
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Priority to AU2014203059A priority Critical patent/AU2014203059B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2010276236A 2009-07-21 2010-07-21 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range Ceased AU2010276236B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2014203059A AU2014203059B2 (en) 2009-07-21 2014-06-05 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22733909P 2009-07-21 2009-07-21
US61/227,339 2009-07-21
US23093809P 2009-08-03 2009-08-03
US61/230,938 2009-08-03
PCT/US2010/042776 WO2011011535A2 (en) 2009-07-21 2010-07-21 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Related Child Applications (1)

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AU2014203059A Division AU2014203059B2 (en) 2009-07-21 2014-06-05 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Publications (2)

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AU2010276236A1 AU2010276236A1 (en) 2012-02-16
AU2010276236B2 true AU2010276236B2 (en) 2014-03-20

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AU2010276236A Ceased AU2010276236B2 (en) 2009-07-21 2010-07-21 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Country Status (6)

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US (5) US8628924B2 (enExample)
EP (3) EP2456887B1 (enExample)
JP (4) JP5575239B2 (enExample)
AU (1) AU2010276236B2 (enExample)
CA (1) CA2768391C (enExample)
WO (1) WO2011011535A2 (enExample)

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ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
US9133490B2 (en) * 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
EP2749654A1 (en) * 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analysis of composition of nucleic acid mixtures
JP6491458B2 (ja) * 2014-10-31 2019-03-27 シスメックス株式会社 核酸増幅方法
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
WO2019002228A1 (en) * 2017-06-26 2019-01-03 Institut Pasteur TREATMENTS TO REMOVE HIV RESERVOIRS AND REDUCE VIRAL LOAD
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
ES2944360T3 (es) * 2017-10-03 2023-06-20 Abbott Molecular Inc Ensayo para la detección del virus de la inmunodeficiencia humana (VIH)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121364A1 (en) * 2000-02-07 2004-06-24 Mark Chee Multiplex nucleic acid reactions

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Also Published As

Publication number Publication date
JP2013500013A (ja) 2013-01-07
JP2014030431A (ja) 2014-02-20
WO2011011535A3 (en) 2011-04-14
US8932817B2 (en) 2015-01-13
EP3330385A1 (en) 2018-06-06
EP3018218A1 (en) 2016-05-11
JP2015186487A (ja) 2015-10-29
US20160258009A1 (en) 2016-09-08
US9347098B2 (en) 2016-05-24
CA2768391A1 (en) 2011-01-27
US20110020824A1 (en) 2011-01-27
WO2011011535A2 (en) 2011-01-27
US20150093753A1 (en) 2015-04-02
CA2768391C (en) 2016-09-06
US20140072974A1 (en) 2014-03-13
EP2456887A2 (en) 2012-05-30
US20180216172A1 (en) 2018-08-02
EP3018218B1 (en) 2018-01-24
JP2017148080A (ja) 2017-08-31
AU2010276236A1 (en) 2012-02-16
JP5863752B2 (ja) 2016-02-17
US8628924B2 (en) 2014-01-14
JP5575239B2 (ja) 2014-08-20
EP2456887B1 (en) 2015-11-25
US9856527B2 (en) 2018-01-02

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