AU2014203059B2 - Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range - Google Patents

Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

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Publication number
AU2014203059B2
AU2014203059B2 AU2014203059A AU2014203059A AU2014203059B2 AU 2014203059 B2 AU2014203059 B2 AU 2014203059B2 AU 2014203059 A AU2014203059 A AU 2014203059A AU 2014203059 A AU2014203059 A AU 2014203059A AU 2014203059 B2 AU2014203059 B2 AU 2014203059B2
Authority
AU
Australia
Prior art keywords
dynamic range
compositions
nucleic acid
methods
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2014203059A
Other versions
AU2014203059A1 (en
Inventor
Kenneth A. Browne
Daniel L. Kacian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2010276236A external-priority patent/AU2010276236B2/en
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Priority to AU2014203059A priority Critical patent/AU2014203059B2/en
Publication of AU2014203059A1 publication Critical patent/AU2014203059A1/en
Priority to AU2016225806A priority patent/AU2016225806A1/en
Application granted granted Critical
Publication of AU2014203059B2 publication Critical patent/AU2014203059B2/en
Priority to AU2018214081A priority patent/AU2018214081A1/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

METHODS AND COMPOSITIONS FOR QUANTITATIVE DETECTION OF NUCLEIC ACID SEQUENCES OVER AN EXTENDED DYNAMIC RANGE Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
AU2014203059A 2009-07-21 2014-06-05 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range Ceased AU2014203059B2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2014203059A AU2014203059B2 (en) 2009-07-21 2014-06-05 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
AU2016225806A AU2016225806A1 (en) 2009-07-21 2016-09-06 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
AU2018214081A AU2018214081A1 (en) 2009-07-21 2018-08-09 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61/227,339 2009-07-21
US61/230,938 2009-08-03
AU2010276236A AU2010276236B2 (en) 2009-07-21 2010-07-21 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
AU2014203059A AU2014203059B2 (en) 2009-07-21 2014-06-05 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
AU2010276236A Division AU2010276236B2 (en) 2009-07-21 2010-07-21 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2016225806A Division AU2016225806A1 (en) 2009-07-21 2016-09-06 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Publications (2)

Publication Number Publication Date
AU2014203059A1 AU2014203059A1 (en) 2014-07-03
AU2014203059B2 true AU2014203059B2 (en) 2016-09-22

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ID=51228765

Family Applications (1)

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AU2014203059A Ceased AU2014203059B2 (en) 2009-07-21 2014-06-05 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Country Status (1)

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AU (1) AU2014203059B2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090181389A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc., A California Corporation Quantitative measurement of nucleic acid via ligation-based linear amplification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090181389A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc., A California Corporation Quantitative measurement of nucleic acid via ligation-based linear amplification

Also Published As

Publication number Publication date
AU2014203059A1 (en) 2014-07-03

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FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired