AU2014203059B2 - Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range - Google Patents
Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic rangeInfo
- Publication number
- AU2014203059B2 AU2014203059B2 AU2014203059A AU2014203059A AU2014203059B2 AU 2014203059 B2 AU2014203059 B2 AU 2014203059B2 AU 2014203059 A AU2014203059 A AU 2014203059A AU 2014203059 A AU2014203059 A AU 2014203059A AU 2014203059 B2 AU2014203059 B2 AU 2014203059B2
- Authority
- AU
- Australia
- Prior art keywords
- dynamic range
- compositions
- nucleic acid
- methods
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000001514 detection method Methods 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical group 0.000 title abstract 3
- 108091093088 Amplicon Proteins 0.000 abstract 5
- 230000003321 amplification Effects 0.000 abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METHODS AND COMPOSITIONS FOR QUANTITATIVE DETECTION OF NUCLEIC ACID SEQUENCES OVER AN EXTENDED DYNAMIC RANGE Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2014203059A AU2014203059B2 (en) | 2009-07-21 | 2014-06-05 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
AU2016225806A AU2016225806A1 (en) | 2009-07-21 | 2016-09-06 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
AU2018214081A AU2018214081A1 (en) | 2009-07-21 | 2018-08-09 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61/227,339 | 2009-07-21 | ||
US61/230,938 | 2009-08-03 | ||
AU2010276236A AU2010276236B2 (en) | 2009-07-21 | 2010-07-21 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
AU2014203059A AU2014203059B2 (en) | 2009-07-21 | 2014-06-05 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2010276236A Division AU2010276236B2 (en) | 2009-07-21 | 2010-07-21 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2016225806A Division AU2016225806A1 (en) | 2009-07-21 | 2016-09-06 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2014203059A1 AU2014203059A1 (en) | 2014-07-03 |
AU2014203059B2 true AU2014203059B2 (en) | 2016-09-22 |
Family
ID=51228765
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2014203059A Ceased AU2014203059B2 (en) | 2009-07-21 | 2014-06-05 | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
Country Status (1)
Country | Link |
---|---|
AU (1) | AU2014203059B2 (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090181389A1 (en) * | 2008-01-11 | 2009-07-16 | Signosis, Inc., A California Corporation | Quantitative measurement of nucleic acid via ligation-based linear amplification |
-
2014
- 2014-06-05 AU AU2014203059A patent/AU2014203059B2/en not_active Ceased
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090181389A1 (en) * | 2008-01-11 | 2009-07-16 | Signosis, Inc., A California Corporation | Quantitative measurement of nucleic acid via ligation-based linear amplification |
Also Published As
Publication number | Publication date |
---|---|
AU2014203059A1 (en) | 2014-07-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FGA | Letters patent sealed or granted (standard patent) | ||
MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |