AU2005317279C1 - Purification of immunoglobulins - Google Patents

Purification of immunoglobulins Download PDF

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Publication number
AU2005317279C1
AU2005317279C1 AU2005317279A AU2005317279A AU2005317279C1 AU 2005317279 C1 AU2005317279 C1 AU 2005317279C1 AU 2005317279 A AU2005317279 A AU 2005317279A AU 2005317279 A AU2005317279 A AU 2005317279A AU 2005317279 C1 AU2005317279 C1 AU 2005317279C1
Authority
AU
Australia
Prior art keywords
separation matrix
antibodies
protein
antibody
column
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU2005317279A
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English (en)
Other versions
AU2005317279B2 (en
AU2005317279A1 (en
Inventor
Per-Mikael Aberg
Hans Berg
Hans J. Johansson
Gunnar Malmquist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
Cytiva Bioprocess R&D AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36588164&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2005317279(C1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from SE0403057A external-priority patent/SE0403057D0/sv
Application filed by Cytiva Bioprocess R&D AB filed Critical Cytiva Bioprocess R&D AB
Publication of AU2005317279A1 publication Critical patent/AU2005317279A1/en
Publication of AU2005317279B2 publication Critical patent/AU2005317279B2/en
Application granted granted Critical
Publication of AU2005317279C1 publication Critical patent/AU2005317279C1/en
Assigned to GE HEALTHCARE BIOPROCESS R&D AB reassignment GE HEALTHCARE BIOPROCESS R&D AB Request for Assignment Assignors: GE HEALTHCARE BIO-SCIENCES AB
Assigned to CYTIVA BIOPROCESS R&D AB reassignment CYTIVA BIOPROCESS R&D AB Request to Amend Deed and Register Assignors: GE HEALTHCARE BIOPROCESS R&D AB
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28004—Sorbent size or size distribution, e.g. particle size
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AU2005317279A 2004-12-14 2005-12-12 Purification of immunoglobulins Expired AU2005317279C1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE0403057-3 2004-12-14
SE0403057A SE0403057D0 (sv) 2004-12-14 2004-12-14 Purification of immunoglobulins
US63831604P 2004-12-22 2004-12-22
US60/638,316 2004-12-22
PCT/SE2005/001900 WO2006065208A1 (en) 2004-12-14 2005-12-12 Purification of immunoglobulins

Publications (3)

Publication Number Publication Date
AU2005317279A1 AU2005317279A1 (en) 2006-06-22
AU2005317279B2 AU2005317279B2 (en) 2011-02-24
AU2005317279C1 true AU2005317279C1 (en) 2014-07-17

Family

ID=36588164

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005317279A Expired AU2005317279C1 (en) 2004-12-14 2005-12-12 Purification of immunoglobulins

Country Status (5)

Country Link
EP (1) EP1830947A4 (enExample)
JP (1) JP5787461B2 (enExample)
AU (1) AU2005317279C1 (enExample)
CA (1) CA2586803C (enExample)
WO (1) WO2006065208A1 (enExample)

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JP5620626B2 (ja) 2005-03-31 2014-11-05 中外製薬株式会社 会合制御によるポリペプチド製造方法
DK2006381T3 (en) 2006-03-31 2016-02-22 Chugai Pharmaceutical Co Ltd PROCEDURE FOR REGULATING ANTIBODIES BLOOD PHARMACOKINETICS
EP2009101B1 (en) 2006-03-31 2017-10-25 Chugai Seiyaku Kabushiki Kaisha Antibody modification method for purifying bispecific antibody
JP5345539B2 (ja) * 2006-09-29 2013-11-20 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー 抗体単離のためのスタフィロコッカスアウレウス由来のドメインｃを含むクロマトグラフィーリガンド
SG149759A1 (en) * 2007-07-10 2009-02-27 Millipore Corp Media for affinity chromatography
ES2687808T3 (es) 2007-09-26 2018-10-29 Chugai Seiyaku Kabushiki Kaisha Región constante de anticuerpo modificado
DK2202245T3 (en) 2007-09-26 2016-11-21 Chugai Pharmaceutical Co Ltd A method of modifying an antibody isoelectric point VIA amino acid substitution in CDR
US8277649B2 (en) * 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
EP2543730B1 (en) 2010-03-04 2018-10-31 Chugai Seiyaku Kabushiki Kaisha Antibody constant region variant
CA2791918C (en) 2010-03-05 2019-11-19 Boehringer Ingelheim International Gmbh Selective enrichment of antibodies
CN102388061A (zh) * 2010-03-17 2012-03-21 松下电器产业株式会社 将由a蛋白或a蛋白的a至e结构域中的至少一个结构域所组成的蛋白质结合到基材上的方法
US8859294B2 (en) 2010-03-17 2014-10-14 Panasonic Corporation Method for binding a protein consisting of protein A or consisting of at least one domain of the A to E domains of the protein A to the substrate
US9090665B2 (en) 2010-03-31 2015-07-28 Jsr Corporation Filler for affinity chromatography
JP5998050B2 (ja) 2010-03-31 2016-09-28 Jsr株式会社 アフィニティークロマトグラフィー用充填剤
TR201802772T4 (tr) 2010-11-17 2018-03-21 Chugai Pharmaceutical Co Ltd Kan pıhtılaşma faktörü VIII in işlevi için alternatif işleve sahip multi-spesifik antijen bağlayıcı molekül.
LT2647707T (lt) 2010-11-30 2018-11-12 Chugai Seiyaku Kabushiki Kaisha Citotoksiškumą indukuojantis terapinis agentas
WO2015030094A1 (ja) 2013-08-30 2015-03-05 株式会社カネカ Ｆａｂ領域結合性ペプチド
WO2015035076A1 (en) * 2013-09-04 2015-03-12 Sten Ohlson Weak affinity chromatography
JP6230853B2 (ja) * 2013-09-06 2017-11-15 株式会社日立製作所 タンパク質の精製方法及び精製装置
ES2881306T3 (es) 2013-09-27 2021-11-29 Chugai Pharmaceutical Co Ltd Método para la producción de heteromultímeros de polipéptidos
US20170043320A1 (en) 2013-11-27 2017-02-16 Jsr Corporation Solid-phase carrier, production method for solid-phase carrier, carrier for affinity refining, production method for filler for affinity chromatography, filler for affinity chromatography, chromatography column, and refining method
EP3094391B1 (en) 2014-01-17 2024-11-20 Repligen Corporation Sterilizing chromatography columns
CN119751653A (zh) 2014-06-24 2025-04-04 思拓凡生物工艺研发有限公司 层析方法
KR102410991B1 (ko) 2014-06-27 2022-06-17 제이에스알 가부시끼가이샤 친화성 크로마토그래피용 담체
US20170334947A1 (en) * 2014-08-28 2017-11-23 Kaneka Corporation Affinity separation matrix for fab region-containing peptide
JPWO2016121701A1 (ja) 2015-01-26 2017-11-09 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
WO2016159213A1 (ja) 2015-04-01 2016-10-06 中外製薬株式会社 ポリペプチド異種多量体の製造方法
JP7219005B2 (ja) * 2015-12-28 2023-02-07 中外製薬株式会社 Ｆｃ領域含有ポリペプチドの精製を効率化するための方法
JP7320943B2 (ja) 2016-04-28 2023-08-04 中外製薬株式会社 抗体含有製剤
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
WO2017195641A1 (ja) * 2016-05-11 2017-11-16 株式会社カネカ アフィニティ分離マトリックスの製造方法およびアフィニティ分離マトリックス
GB201703116D0 (en) * 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
AU2018361430B2 (en) 2017-11-01 2025-08-14 Chugai Seiyaku Kabushiki Kaisha Antibody variant and isoform with lowered biological activity

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5089605A (en) * 1987-03-13 1992-02-18 Repligen Corporation Immobilized immunoglobulin-binding proteins
US5672276A (en) * 1992-06-19 1997-09-30 Biosepra Inc. Passivated porous polymer supports and methods for the preparation and use of same
US5907016A (en) * 1993-03-29 1999-05-25 Virginia Tech Intellectual Properties,Inc. Method of coupling ligands onto supports and products produced therefrom
EP0955332A1 (en) * 1997-01-07 1999-11-10 Kaneka Corporation Cellulosic particles, spherical object comprising cross-linked polymer particles, and adsorbent for body fluid purification
WO2002053254A1 (en) * 2000-12-29 2002-07-11 Amersham Biosciences Ab A method for producing liquid chromatography matrices
US6428707B1 (en) * 1997-02-04 2002-08-06 Amersham Pharmacia Biotech Ab Adsorption/separation method and a medium for adsorption/separation
US6602990B1 (en) * 1996-04-11 2003-08-05 Amersham Biosciences Ab Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography
WO2003080655A1 (en) * 2002-03-25 2003-10-02 Amersham Biosciences Ab A mutated immunoglobulin-binding protein
WO2004074211A1 (en) * 2003-02-21 2004-09-02 Amersham Biosciences Ab Inorganic beads with hierarchical pore structures
EP1500431A2 (en) * 2003-07-22 2005-01-26 Millipore (U.K.) Ltd. Immobilised affinity medium and its use in separation
WO2006024497A1 (en) * 2004-08-30 2006-03-09 Lonza Biologics Plc. Affinity- plus ion exchange- chromatography for purifying antibodies

Family Cites Families (1)

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SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5089605A (en) * 1987-03-13 1992-02-18 Repligen Corporation Immobilized immunoglobulin-binding proteins
US5672276A (en) * 1992-06-19 1997-09-30 Biosepra Inc. Passivated porous polymer supports and methods for the preparation and use of same
US5907016A (en) * 1993-03-29 1999-05-25 Virginia Tech Intellectual Properties,Inc. Method of coupling ligands onto supports and products produced therefrom
US6602990B1 (en) * 1996-04-11 2003-08-05 Amersham Biosciences Ab Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography
EP0955332A1 (en) * 1997-01-07 1999-11-10 Kaneka Corporation Cellulosic particles, spherical object comprising cross-linked polymer particles, and adsorbent for body fluid purification
US6428707B1 (en) * 1997-02-04 2002-08-06 Amersham Pharmacia Biotech Ab Adsorption/separation method and a medium for adsorption/separation
WO2002053254A1 (en) * 2000-12-29 2002-07-11 Amersham Biosciences Ab A method for producing liquid chromatography matrices
WO2003080655A1 (en) * 2002-03-25 2003-10-02 Amersham Biosciences Ab A mutated immunoglobulin-binding protein
WO2004074211A1 (en) * 2003-02-21 2004-09-02 Amersham Biosciences Ab Inorganic beads with hierarchical pore structures
EP1500431A2 (en) * 2003-07-22 2005-01-26 Millipore (U.K.) Ltd. Immobilised affinity medium and its use in separation
WO2006024497A1 (en) * 2004-08-30 2006-03-09 Lonza Biologics Plc. Affinity- plus ion exchange- chromatography for purifying antibodies

Also Published As

Publication number Publication date
EP1830947A4 (en) 2012-04-18
CA2586803A1 (en) 2006-06-22
AU2005317279B2 (en) 2011-02-24
AU2005317279A1 (en) 2006-06-22
EP1830947A1 (en) 2007-09-12
WO2006065208A1 (en) 2006-06-22
CA2586803C (en) 2012-12-11
JP2008523140A (ja) 2008-07-03
JP5787461B2 (ja) 2015-09-30

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Legal Events

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CB Opposition filed

Opponent name: STEPHEN DEAN SHARP AND DAVID JOHN HUGHES

ON Decision of a delegate or deputy of the commissioner of patents (result of patent office hearing)

Free format text: APO 10: LACK OF CLARITY NOT ESTABLISHED - FAIR BASIS, CLAIMS NEED NOT BE LIMITED TO THE PREFERRED EMBODIMENTS - LACK OF FAIR BASIS NOT ESTABLISHED.

Opponent name: STEPHEN DEAN SHARP DAVID JOHN HUGHES

DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 13 MAR 2014 .

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 13 MAR 2014

FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: GE HEALTHCARE BIOPROCESS R&D AB

Free format text: FORMER OWNER WAS: GE HEALTHCARE BIO-SCIENCES AB

HB Alteration of name in register

Owner name: CYTIVA BIOPROCESS R&D AB

Free format text: FORMER NAME(S): GE HEALTHCARE BIOPROCESS R&D AB