AU2005245948B2 - Use of whole blood in PCR reactions - Google Patents
Use of whole blood in PCR reactions Download PDFInfo
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- AU2005245948B2 AU2005245948B2 AU2005245948A AU2005245948A AU2005245948B2 AU 2005245948 B2 AU2005245948 B2 AU 2005245948B2 AU 2005245948 A AU2005245948 A AU 2005245948A AU 2005245948 A AU2005245948 A AU 2005245948A AU 2005245948 B2 AU2005245948 B2 AU 2005245948B2
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
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- Life Sciences & Earth Sciences (AREA)
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- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/850,816 US20050260606A1 (en) | 2004-05-20 | 2004-05-20 | Use of whole blood in PCR reactions |
| US10/850,816 | 2004-05-20 | ||
| US11/005,559 US7462475B2 (en) | 2004-05-20 | 2004-12-06 | Use of whole blood in PCR reactions |
| US11/005,559 | 2004-12-06 | ||
| PCT/US2005/017669 WO2005113829A2 (en) | 2004-05-20 | 2005-05-20 | Use of whole blood in pcr reactions |
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| AU2005245948A1 AU2005245948A1 (en) | 2005-12-01 |
| AU2005245948B2 true AU2005245948B2 (en) | 2011-04-07 |
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| DK (1) | DK1747290T3 (enExample) |
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| US7462475B2 (en) | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
| EP1880206B1 (en) * | 2005-05-09 | 2012-11-14 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| CA2607221A1 (en) | 2005-05-12 | 2006-11-23 | Panomics, Inc. | Multiplex branched-chain dna assays |
| CN101495650B (zh) * | 2005-06-20 | 2015-02-04 | 领先细胞医疗诊断有限公司 | 检测单个细胞中的核酸和鉴定异质大细胞群中罕见细胞的方法 |
| US7927798B2 (en) * | 2005-10-05 | 2011-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
| EP2061894A4 (en) * | 2006-09-14 | 2010-05-05 | Dna Polymerase Technology Inc | USE OF MUTANT TAQ POLYMERASE ENZYMES FOR DNA AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS |
| JP5292722B2 (ja) * | 2007-05-09 | 2013-09-18 | 凸版印刷株式会社 | 未精製血液をサンプルとする核酸増幅法 |
| WO2009140497A1 (en) * | 2008-05-16 | 2009-11-19 | New England Biolabs, Inc. | Enzyme reagents for amplification of polynucleotides in the presence of inhibitors |
| FI20095729A0 (fi) * | 2009-06-26 | 2009-06-26 | Finnzymes Oy | Menetelmä deoksiribonukleiinihappojen kvantitatiiviseen PCR-monistukseen PCR-inhibiittoreita sisältävästä näytteestä |
| ES2648564T3 (es) | 2010-10-21 | 2018-01-04 | Advanced Cell Diagnostics, Inc. | Método ultrasensible para la detección in situ de ácidos nucleicos |
| US8409807B2 (en) | 2010-10-22 | 2013-04-02 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
| US8563298B2 (en) | 2010-10-22 | 2013-10-22 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
| US9714940B2 (en) | 2010-10-22 | 2017-07-25 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
| US9017979B2 (en) | 2011-04-11 | 2015-04-28 | Roche Molecular Systems, Inc. | DNA polymerases with improved activity |
| GB201113430D0 (en) | 2011-08-03 | 2011-09-21 | Fermentas Uab | DNA polymerases |
| US11208636B2 (en) | 2011-08-10 | 2021-12-28 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| EP2742151B1 (en) | 2011-08-10 | 2017-10-25 | Life Technologies Corporation | Polymerase compositions |
| CA2858264C (en) | 2011-12-08 | 2018-01-02 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| US9562271B2 (en) | 2012-04-20 | 2017-02-07 | T2 Biosystems, Inc. | Compositions and methods for detection of Candida species |
| US10683537B2 (en) * | 2012-10-16 | 2020-06-16 | Dna Polymerase Technology, Inc. | Inhibition-resistant polymerases |
| GB201219137D0 (en) * | 2012-10-24 | 2012-12-05 | Ge Healthcare Uk Ltd | Direct nucleic acid amplification kit, reagent and method |
| WO2016118766A2 (en) | 2015-01-21 | 2016-07-28 | T2 Biosystems, Inc. | Nmr methods and systems for the rapid detection of tick-borne pathogens |
| WO2016183294A1 (en) | 2015-05-12 | 2016-11-17 | Dna Polymerase Technology, Inc. | Mutant polymerases and uses thereof |
| DK3362462T3 (da) | 2015-10-12 | 2021-10-11 | Advanced Cell Diagnostics Inc | In situ-detektion af nukleotidvarianter i prøver med højt støjniveau, og sammensætninger og fremgangsmåder relateret dertil |
| WO2017090684A1 (ja) * | 2015-11-27 | 2017-06-01 | 国立大学法人九州大学 | Ｄｎａポリメラーゼ変異体 |
| EP3405559B1 (en) | 2016-01-21 | 2021-11-17 | T2 Biosystems, Inc. | Rapid antimicrobial susceptibility testing using high-sensitivity direct detection methods |
| CA3011901A1 (en) | 2016-01-21 | 2017-07-27 | T2 Biosystems, Inc. | Nmr methods and systems for the rapid detection of bacteria |
| WO2017180745A1 (en) | 2016-04-14 | 2017-10-19 | T2 Biosystems, Inc. | Methods and systems for amplification in complex samples |
| US10626444B2 (en) * | 2016-10-14 | 2020-04-21 | ACUDX Inc. | Methods of assaying nucleic acids of low quantities using a buffer nucleic acid |
| JP6730525B2 (ja) | 2016-11-21 | 2020-07-29 | ナノストリング テクノロジーズ，インコーポレイティド | 化学組成物とそれを利用する方法 |
| WO2019143622A1 (en) | 2018-01-19 | 2019-07-25 | Bio-Rad Laboratories, Inc. | Mutant dna polymerases |
| JP7525402B2 (ja) | 2018-05-14 | 2024-07-30 | ナノストリング テクノロジーズ，インコーポレイティド | 化学的組成物とそれを利用する方法 |
| CN109022386B (zh) * | 2018-07-12 | 2020-11-03 | 深圳市华中生物药械有限公司 | 重组Taq直扩酶及其制备方法、重组质粒和工程菌 |
| US20220090194A1 (en) * | 2019-01-09 | 2022-03-24 | 3Cr Bioscience Ltd. | Method for detecting a nucleic acid sequence |
| GB2587177A (en) * | 2019-01-09 | 2021-03-24 | 3Cr Bioscience Ltd | Polymerase |
| CN110295237B (zh) * | 2019-06-27 | 2023-07-25 | 河北农业大学 | 一种改进的鸡隐性白羽基因型的分子鉴定方法 |
| US20230304082A1 (en) * | 2020-05-29 | 2023-09-28 | Genotech Corp. | Dna polymerase variant with improved discrimination of genetic variants |
| CN114134220A (zh) * | 2022-01-30 | 2022-03-04 | 济凡生物科技（北京）有限公司 | 一种可以用于血液检测的pcr反应液及其试剂盒 |
Family Cites Families (12)
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| US5466591A (en) * | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| EP0553264A4 (en) | 1990-10-05 | 1994-07-13 | Wayne M Barnes | Thermostable dna polymerase |
| ES2194843T3 (es) * | 1992-09-11 | 2003-12-01 | Hoffmann La Roche | Deteccion de acidos nucleicos en sangre. |
| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US6818431B1 (en) * | 1995-10-18 | 2004-11-16 | Shanghai Mendel Dna Center Co. Ltd. | DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides |
| WO1999054455A1 (fr) * | 1998-04-23 | 1999-10-28 | Takara Shuzo Co., Ltd. | Procede de synthese de l'adn |
| US6214557B1 (en) * | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| US6428962B1 (en) * | 2001-02-12 | 2002-08-06 | Dna Analysis, Inc. | Nucleic acid collection barrier method and apparatus |
| US6896872B2 (en) | 2001-06-27 | 2005-05-24 | Svetlana A. Dambinova | Rapid multiple panel of biomarkers in laboratory blood tests for TIA/stroke |
| WO2004013279A2 (en) * | 2002-05-14 | 2004-02-12 | Fidelity Systems, Inc. | Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes |
| US7462475B2 (en) | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
| EP2061894A4 (en) | 2006-09-14 | 2010-05-05 | Dna Polymerase Technology Inc | USE OF MUTANT TAQ POLYMERASE ENZYMES FOR DNA AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS |
-
2004
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2005
- 2005-05-20 DK DK05759554.8T patent/DK1747290T3/da active
- 2005-05-20 AU AU2005245948A patent/AU2005245948B2/en not_active Expired - Fee Related
- 2005-05-20 WO PCT/US2005/017669 patent/WO2005113829A2/en not_active Ceased
- 2005-05-20 CA CA2567620A patent/CA2567620C/en not_active Expired - Lifetime
- 2005-05-20 JP JP2007527461A patent/JP2008506417A/ja active Pending
- 2005-05-20 EP EP05759554.8A patent/EP1747290B1/en not_active Expired - Lifetime
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2008
- 2008-12-08 US US12/330,201 patent/US8470563B2/en not_active Expired - Fee Related
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2013
- 2013-06-01 US US13/907,891 patent/US20130252309A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| Kermekchiev, MB et al., Nucleic Acids Research, vol 31, pages 6139-6147 * |
Also Published As
| Publication number | Publication date |
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| US7462475B2 (en) | 2008-12-09 |
| CA2567620C (en) | 2016-09-20 |
| EP1747290B1 (en) | 2014-05-07 |
| AU2005245948A1 (en) | 2005-12-01 |
| EP1747290A4 (en) | 2009-01-14 |
| CA2567620A1 (en) | 2005-12-01 |
| US20090170060A1 (en) | 2009-07-02 |
| US20060084074A1 (en) | 2006-04-20 |
| WO2005113829A3 (en) | 2008-04-03 |
| WO2005113829A2 (en) | 2005-12-01 |
| EP1747290A2 (en) | 2007-01-31 |
| DK1747290T3 (da) | 2014-08-11 |
| US20130252309A1 (en) | 2013-09-26 |
| JP2008506417A (ja) | 2008-03-06 |
| US8470563B2 (en) | 2013-06-25 |
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| FGA | Letters patent sealed or granted (standard patent) | ||
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