AU2004242082B2 - URA5 gene and methods for stable genetic integration in yeast - Google Patents

URA5 gene and methods for stable genetic integration in yeast Download PDF

Info

Publication number
AU2004242082B2
AU2004242082B2 AU2004242082A AU2004242082A AU2004242082B2 AU 2004242082 B2 AU2004242082 B2 AU 2004242082B2 AU 2004242082 A AU2004242082 A AU 2004242082A AU 2004242082 A AU2004242082 A AU 2004242082A AU 2004242082 B2 AU2004242082 B2 AU 2004242082B2
Authority
AU
Australia
Prior art keywords
nucleic acid
acid sequence
sequence
seq
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2004242082A
Other languages
English (en)
Other versions
AU2004242082A1 (en
Inventor
Juergen H. Nett
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlycoFi Inc
Original Assignee
GlycoFi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GlycoFi Inc filed Critical GlycoFi Inc
Publication of AU2004242082A1 publication Critical patent/AU2004242082A1/en
Application granted granted Critical
Publication of AU2004242082B2 publication Critical patent/AU2004242082B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2004242082A 2003-05-16 2004-04-30 URA5 gene and methods for stable genetic integration in yeast Ceased AU2004242082B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US47143503P 2003-05-16 2003-05-16
US60/471,435 2003-05-16
US10/454,125 2003-06-03
US10/454,125 US7514253B2 (en) 2003-05-16 2003-06-03 URA5 gene and methods for stable genetic integration in yeast
PCT/US2004/013488 WO2004104165A2 (en) 2003-05-16 2004-04-30 Ura5 gene and methods for stable genetic integration in yeast

Publications (2)

Publication Number Publication Date
AU2004242082A1 AU2004242082A1 (en) 2004-12-02
AU2004242082B2 true AU2004242082B2 (en) 2008-12-11

Family

ID=33423332

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004242082A Ceased AU2004242082B2 (en) 2003-05-16 2004-04-30 URA5 gene and methods for stable genetic integration in yeast

Country Status (6)

Country Link
US (5) US7514253B2 (cg-RX-API-DMAC7.html)
EP (2) EP1633855A4 (cg-RX-API-DMAC7.html)
JP (2) JP2007530007A (cg-RX-API-DMAC7.html)
AU (1) AU2004242082B2 (cg-RX-API-DMAC7.html)
CA (1) CA2525954A1 (cg-RX-API-DMAC7.html)
WO (1) WO2004104165A2 (cg-RX-API-DMAC7.html)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
CA2651456A1 (en) * 2006-05-19 2007-11-29 Glycofi, Inc. Recombinant vectors
EP2235198B1 (en) * 2007-12-19 2018-08-29 GlycoFi, Inc. Yeast strains for protein production
JP2011514157A (ja) * 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
JP2010017131A (ja) * 2008-07-10 2010-01-28 Mitsui Eng & Shipbuild Co Ltd モーレラ属細菌及びプライマー
WO2010019487A1 (en) 2008-08-12 2010-02-18 Glycofi, Inc. Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
EP2401378B1 (en) 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
JP2013507921A (ja) 2009-10-16 2013-03-07 メルク・シャープ・エンド・ドーム・コーポレイション 宿主細胞抗原に対する抗体に対する検出可能な交差結合活性を欠くタンパク質の、ピチア・パストリスにおける製造方法
BR112012020882A2 (pt) 2010-02-24 2015-11-03 Merck Sharp & Dohme métodos para produzir uma glicoproteína heteróloga e para produzir uma composição de glicoproteína, célula hospedeira, composição de glicoproteína, e, uso da pichia pastoris recombinante.
JP5733570B2 (ja) 2011-05-23 2015-06-10 ソニー株式会社 画像処理装置、画像処理方法、プログラム、および、記録媒体
EP2925345B1 (en) 2012-12-03 2018-09-05 Merck Sharp & Dohme Corp. Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
US9944689B2 (en) 2013-03-07 2018-04-17 The General Hospital Corporation Human CTLA4 mutants and use thereof
JP6488666B2 (ja) * 2013-11-22 2019-03-27 Ａｇｃ株式会社 クローニングベクター、発現ベクターおよび形質転換体の製造方法
EP3072955B1 (en) 2013-11-22 2019-01-09 JMTC Enzyme Corporation Transformant and process for production thereof, and process for production of lactic acid
US10214754B2 (en) 2014-10-10 2019-02-26 Jmtc Enzyme Corporation Transformant and its production process, and method for producing lactic acid
US12421316B2 (en) 2019-02-19 2025-09-23 The Regents Of The University Of Colorado, A Body Corporate Bispecific immunotoxins targeting human CD25+CCR4+ tumors and regulatory T-cells
CN111850017A (zh) * 2019-04-30 2020-10-30 广州华真医药科技有限公司 一种基于ura3基因的表达载体及其构建方法
CN115838645B (zh) * 2022-09-15 2024-03-08 天津大学 一种高产乳清酸的酵母菌株及其应用
GB2635858A (en) * 2024-11-20 2025-05-28 Kao Corp Selection marker for gene recombination

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU661844B2 (en) * 1991-04-01 1995-08-10 Merck Sharp & Dohme Corp. Genes which influence (pichia) proteolytic activity, and uses therefor
DE19934408A1 (de) * 1999-07-22 2001-01-25 Consortium Elektrochem Ind pyrF Gen und seine Verwendung

Also Published As

Publication number Publication date
JP2011115182A (ja) 2011-06-16
US20150087013A1 (en) 2015-03-26
US20090203105A1 (en) 2009-08-13
WO2004104165A3 (en) 2007-11-22
EP1633855A4 (en) 2008-08-20
US8062879B2 (en) 2011-11-22
US7514253B2 (en) 2009-04-07
US20040229306A1 (en) 2004-11-18
AU2004242082A1 (en) 2004-12-02
WO2004104165A2 (en) 2004-12-02
EP1633855A2 (en) 2006-03-15
US20120116058A1 (en) 2012-05-10
JP2007530007A (ja) 2007-11-01
CA2525954A1 (en) 2004-12-02
US8524479B2 (en) 2013-09-03
EP2489740A1 (en) 2012-08-22
US20140051172A1 (en) 2014-02-20
WO2004104165A9 (en) 2006-03-02

Similar Documents

Publication Publication Date Title
US8062879B2 (en) URA5 gene and methods for stable genetic integration in yeast
EP1696864B1 (en) Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
AU2005238308B8 (en) Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
EP2316963B1 (en) Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
CN101903531B (zh) 用于蛋白质生产的酵母菌株
JP2007534330A5 (cg-RX-API-DMAC7.html)
CA2753408A1 (en) Production of modified glycoproteins having multiple antennary structures
Nett et al. Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris
US7479389B2 (en) ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration
US20120100620A1 (en) Pichia pastoris loci encoding enzymes in the lysine biosynthetic pathway
JP5752582B2 (ja) 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法
CA2876864A1 (en) Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
US20120100622A1 (en) Pichia pastoris loci encoding enzymes in the uracil biosynthetic pathway
US20120100617A1 (en) Pichia pastoris loci encoding enzymes in the adenine biosynthetic pathway

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired