AU2003240810A1 - Methods, compositions, and growth and differentiation factors for insulin-producing cells - Google Patents

Methods, compositions, and growth and differentiation factors for insulin-producing cells Download PDF

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Publication number
AU2003240810A1
AU2003240810A1 AU2003240810A AU2003240810A AU2003240810A1 AU 2003240810 A1 AU2003240810 A1 AU 2003240810A1 AU 2003240810 A AU2003240810 A AU 2003240810A AU 2003240810 A AU2003240810 A AU 2003240810A AU 2003240810 A1 AU2003240810 A1 AU 2003240810A1
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Australia
Prior art keywords
culture medium
factors
cells
column
medium contains
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Abandoned
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AU2003240810A
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English (en)
Inventor
Perry D. Haaland
Mohammad A. Heidaran
Catherine Anne Mcintyre
Sharon C. Presnell
David Scharp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Viacyte Inc
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Becton Dickinson and Co
Novocell Inc
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Application filed by Becton Dickinson and Co, Novocell Inc filed Critical Becton Dickinson and Co
Publication of AU2003240810A1 publication Critical patent/AU2003240810A1/en
Abandoned legal-status Critical Current

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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AU2003240810A 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells Abandoned AU2003240810A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38400002P 2002-05-28 2002-05-28
US60/384,000 2002-05-28
US44373303A 2003-05-22 2003-05-22
US10/443,733 2003-05-22
PCT/US2003/016734 WO2003100026A2 (fr) 2002-05-28 2003-05-28 Procedes, compositions et facteurs de croissance et de differenciation pour cellules productrices d'insuline

Publications (1)

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AU2003240810A1 true AU2003240810A1 (en) 2003-12-12

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AU2003240810A Abandoned AU2003240810A1 (en) 2002-05-28 2003-05-28 Methods, compositions, and growth and differentiation factors for insulin-producing cells

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EP (1) EP1507552A2 (fr)
JP (1) JP2006506047A (fr)
CN (1) CN1668324A (fr)
AU (1) AU2003240810A1 (fr)
BR (1) BR0311362A (fr)
CA (1) CA2487858A1 (fr)
WO (1) WO2003100026A2 (fr)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2457999T (pt) * 2002-12-16 2019-01-28 Technion Res & Dev Foundation Sistema de cultura para células estaminais pluripotentes
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
US8586357B2 (en) 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
WO2005063971A2 (fr) 2003-12-23 2005-07-14 Cythera, Inc Endoderme definitif
EP2377922B1 (fr) * 2004-04-27 2020-04-08 Viacyte, Inc. Endoderme exprimant PDX1
WO2006001471A1 (fr) * 2004-06-29 2006-01-05 The University Of Tokyo Composition medicale favorisant la secretion d'insuline en reponse au glucose
JP5687816B2 (ja) * 2004-07-09 2015-03-25 ヴィアサイト，インコーポレイテッド 胚体内胚葉を分化させるための因子を同定する方法
MX2007001772A (es) 2004-08-13 2007-07-11 Univ Georgia Res Found Composiciones y metodos para auto-renovacion y diferenciacion de celulas troncales embrionicas humanas.
WO2006054305A2 (fr) * 2004-11-22 2006-05-26 Ramot At Tel Aviv University Ltd. Populations de cellules beta adultes d'ilot de langerhans etendues et redifferenciees permettant de produire de l'insuline et procede d'obtention associe
AU2006210955A1 (en) * 2005-01-31 2006-08-10 Es Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
WO2006108651A2 (fr) * 2005-04-12 2006-10-19 Develogen Aktiengesellschaft Utilisation de produits de l'activine pour prevenir et/ou traiter le diabete et/ou le syndrome metabolique
JP4534039B2 (ja) * 2005-04-12 2010-09-01 国立大学法人群馬大学 糖代謝促進剤並びに肥満及び糖尿病治療薬のスクリーニング方法
JP5584875B2 (ja) * 2005-04-13 2014-09-10 国立大学法人 千葉大学 成体膵由来膵幹細胞の製造方法
EP1957636B1 (fr) 2005-10-27 2018-07-04 Viacyte, Inc. Endoderme d'intestin anterieur dorsal et ventral exprimant pdx1
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
JP6110048B2 (ja) 2006-03-02 2017-04-05 ヴィアサイト，インコーポレイテッド 内分泌前駆細胞、膵臓ホルモン発現細胞及びそれらの製造方法
CN101501185A (zh) * 2006-06-09 2009-08-05 人类起源公司 胎盘巢(placental niche)及其培养干细胞的用途
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
CA2743641C (fr) 2008-11-14 2024-03-26 Viacyte, Inc. Encapsulation de cellules pancreatiques derivees de cellules souches pluripotentes humaines
US9328331B2 (en) * 2009-05-21 2016-05-03 Joslin Diabetes Center, Inc. Compositions and methods for promoting beta cell maturity
EP2281874A1 (fr) * 2009-07-31 2011-02-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Production de cellules ß
WO2011071085A1 (fr) * 2009-12-08 2011-06-16 国立大学法人東京大学 Procédé pour produire des cellules induites pour être différenciées à partir de cellules souches pluripotentes
CA2785966C (fr) 2009-12-29 2020-10-27 Takeda Pharmaceutical Company Limited Procede pour la fabrication de cellules de production d'hormones pancreatiques
EP2546334A4 (fr) * 2010-03-12 2014-10-29 Fundación Progreso Y Salud Procédé pour la prolifération "in vitro" de cellules issues de tissus d'origine endodermique
EP2569430B1 (fr) 2010-05-12 2018-10-17 The Trustees of Columbia University in the City of New York Procédés de production de cellules entéroendocrines capables de fabriquer et de sécréter de l'insuline
US9404087B2 (en) 2010-12-15 2016-08-02 Kadimastem Ltd. Insulin producing cells derived from pluripotent stem cells
EP2683812A4 (fr) * 2011-03-07 2014-12-03 Massachusetts Inst Technology Procédés pour transfecter des cellules avec des acides nucléiques
CA2915091A1 (fr) 2013-06-11 2014-12-18 President And Fellows Of Harvard College Cellules ? derivees de cellules souches et compositions et procedes pour generer ces cellules
EP2840132B1 (fr) * 2013-08-22 2016-11-30 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts Universitätsmedizin Procédé pour produire par génie génétique des muscles cardiaques (EHM)
US9404086B2 (en) * 2014-03-26 2016-08-02 Ge Healthcare Uk Limited Method for cell differentiation
US10487314B2 (en) 2014-06-26 2019-11-26 The Trustees Of Columbia University In The City Of New York Inhibition of serotonin expression in gut enteroendocrine cells results in conversion to insulin-positive cells
WO2016100909A1 (fr) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Procédés de génération de cellules β dérivées de cellules souches et leurs utilisations
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
WO2016100898A1 (fr) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Protocole de différentiation dirigée in vitro sans sérum pour générer des cellules b dérivées de cellules souches et leurs utilisations
IT201700066486A1 (it) * 2017-06-15 2018-12-15 Azienda Ospedaliero Univ Policlinico S Orsola Malpighi Bologn A Siero sanguigno per uso nel trattamento di patologie neurodegenerative oftalmologiche
WO2019017492A1 (fr) * 2017-07-20 2019-01-24 国立研究開発法人理化学研究所 Procédé de maturation de tissu rétinien contenant un épithélium continu
WO2019099725A1 (fr) 2017-11-15 2019-05-23 Semma Therapeutics, Inc. Compositions pour la fabrication de cellules d'ilôts et procédés d'utilisation
WO2019142833A1 (fr) * 2018-01-16 2019-07-25 学校法人慶應義塾 PROCÉDÉ DE DÉRIVATION DE CELLULES DE REMPLACEMENT DE L'ENDOTHÉLIUM CORNÉEN À PARTIR DE CELLULES iPS
AU2019320072A1 (en) 2018-08-10 2021-02-25 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
TWI776368B (zh) * 2020-05-29 2022-09-01 國璽幹細胞應用技術股份有限公司 細胞分化培養基組合物、高分泌量胰島素產生細胞及其製備方法
CN115433684A (zh) * 2022-10-20 2022-12-06 云南师范大学 一种盘基网柄菌细胞同步化方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935852A (en) * 1997-07-03 1999-08-10 Genetics Institute, Inc. DNA molecules encoding mammalian cerberus-like proteins

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