AU2003216139B2 - Bioluminescent protease assay - Google Patents

Bioluminescent protease assay Download PDF

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Publication number
AU2003216139B2
AU2003216139B2 AU2003216139A AU2003216139A AU2003216139B2 AU 2003216139 B2 AU2003216139 B2 AU 2003216139B2 AU 2003216139 A AU2003216139 A AU 2003216139A AU 2003216139 A AU2003216139 A AU 2003216139A AU 2003216139 B2 AU2003216139 B2 AU 2003216139B2
Authority
AU
Australia
Prior art keywords
substrate
aminoluciferin
compound
carboxy
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2003216139A
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English (en)
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AU2003216139A1 (en
Inventor
Bill Daily
Dieter Klaubert
Martha O'brien
Keith Wood
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Promega Corp
Original Assignee
Promega Corp
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Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of AU2003216139A1 publication Critical patent/AU2003216139A1/en
Application granted granted Critical
Publication of AU2003216139B2 publication Critical patent/AU2003216139B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62—Benzothiazoles
    • C07D277/64—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
    • C07D277/66—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2 with aromatic rings or ring systems directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AU2003216139A 2002-02-01 2003-01-31 Bioluminescent protease assay Ceased AU2003216139B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35315802P 2002-02-01 2002-02-01
US60/353,158 2002-02-01
PCT/US2003/002936 WO2003066611A1 (en) 2002-02-01 2003-01-31 Bioluminescent protease assay

Publications (2)

Publication Number Publication Date
AU2003216139A1 AU2003216139A1 (en) 2003-09-02
AU2003216139B2 true AU2003216139B2 (en) 2008-08-14

Family

ID=27734277

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003216139A Ceased AU2003216139B2 (en) 2002-02-01 2003-01-31 Bioluminescent protease assay

Country Status (6)

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US (4) US7148030B2 (US20030211560A1-20031113-C00002.png)
EP (2) EP2388254B1 (US20030211560A1-20031113-C00002.png)
JP (1) JP4451663B2 (US20030211560A1-20031113-C00002.png)
AU (1) AU2003216139B2 (US20030211560A1-20031113-C00002.png)
CA (1) CA2474695A1 (US20030211560A1-20031113-C00002.png)
WO (1) WO2003066611A1 (US20030211560A1-20031113-C00002.png)

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US7148030B2 (en) * 2002-02-01 2006-12-12 Promega Corporation Bioluminescent protease assay
WO2004027378A2 (en) * 2002-09-20 2004-04-01 Promega Corporation Luminescence-based methods and probes for measuring cytochrome p450 activity
EP2325329B1 (en) 2002-12-23 2014-02-12 Promega Corporation Improved luciferase-based assays
JP2006517413A (ja) * 2003-02-12 2006-07-27 プロメガ　コーポレイション 光を発光反応から消失させる二重酵素アッセイの方法及びキット
US7416854B2 (en) * 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
EP1935986B1 (en) * 2005-05-31 2014-04-16 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
EP2778234B1 (en) * 2005-05-31 2017-09-27 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
WO2007027653A1 (en) 2005-09-01 2007-03-08 Promega Corporation Cell-based luminogenic and nonluminogenic proteasome assays
JP4899046B2 (ja) * 2005-09-30 2012-03-21 国立大学法人 東京大学 新規ルシフェリン誘導体
GB2435511A (en) * 2006-02-23 2007-08-29 Mologic Ltd Protease detection
GB2435512A (en) * 2006-02-23 2007-08-29 Mologic Ltd A binding assay and assay device
GB2435510A (en) * 2006-02-23 2007-08-29 Mologic Ltd Enzyme detection product and methods
JPWO2007111345A1 (ja) * 2006-03-29 2009-08-13 国立大学法人 東京大学 活性酸素測定用試薬
GB2437311A (en) * 2006-04-07 2007-10-24 Mologic Ltd A protease detection product
WO2008011008A1 (en) * 2006-07-17 2008-01-24 The Trustees Of The University Of Pennsylvania Bioluminescent imaging of trail induced apoptosis
CA2678717A1 (en) 2007-02-28 2008-09-04 Sanofi-Aventis Imaging probes
WO2008118445A1 (en) * 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
US8227205B2 (en) 2007-04-13 2012-07-24 Promega Corporation Luminescent live and dead cell assay
CN101772577A (zh) * 2007-08-03 2010-07-07 塞诺菲-安万特股份有限公司 半胱天冬蛋白酶成像探针
WO2009063840A1 (ja) 2007-11-12 2009-05-22 Hiroshima University エンドトキシンの濃度測定方法および濃度測定用キット
WO2009117539A2 (en) * 2008-03-19 2009-09-24 Abbott Laboratories Bioluminescent construct
US20090238436A1 (en) * 2008-03-19 2009-09-24 Abbott Laboratories Composition for imaging reagent
EP2269064B1 (en) * 2008-03-27 2016-11-30 Promega Corporation Protein labeling with cyanobenzothiazole conjugates
WO2010021686A1 (en) 2008-08-18 2010-02-25 Promega Corporation Luminogenic compounds and methods to detect cytochrome p450 3a enzymes
US20110165611A1 (en) * 2008-09-04 2011-07-07 Chun Li Dual modality detection of apoptosis
WO2010030343A1 (en) * 2008-09-09 2010-03-18 Promega Corporation Bioluminescent assays using cyanobenzothiazole compounds
US8586759B2 (en) * 2010-02-26 2013-11-19 Lawrence Livermore National Security, Llc Methods and systems for synthesis of a D-aminoluciferin precursor and related compounds
JP5905402B2 (ja) * 2010-03-11 2016-04-20 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ シアノベンゾチアゾール化合物を使用する生物発光アッセイ
US20120021923A1 (en) * 2010-05-04 2012-01-26 Contag Christopher H Methods and systems to diagnose a condition in an individual
WO2012061477A1 (en) * 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
SG10202103336SA (en) 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
JP6067019B2 (ja) 2011-09-02 2017-01-25 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 代謝的に活性な細胞の酸化還元状態を評価するための化合物及び方法、ならびにｎａｄ（ｐ）／ｎａｄ（ｐ）ｈを測定するための方法
CN104458709A (zh) * 2013-09-12 2015-03-25 中国药科大学 一种筛选钙激活中性蛋白酶-1抑制剂高通量筛选方法
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
CA3016107A1 (en) 2016-04-06 2017-10-12 Nestec S.A. Biomarkers for predicting degree of weight loss
JP2020150830A (ja) * 2019-03-19 2020-09-24 東亜ディーケーケー株式会社 唾液のプロテアーゼ活性の測定方法、及び口腔内細菌の活動量の評価方法

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WO2002006458A2 (en) * 2000-07-19 2002-01-24 Chemicon International, Inc. Protease specific cleavable luciferases and methods of use thereof

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Also Published As

Publication number Publication date
US20060121546A1 (en) 2006-06-08
US7666987B2 (en) 2010-02-23
WO2003066611A1 (en) 2003-08-14
US8071328B2 (en) 2011-12-06
US7384758B2 (en) 2008-06-10
EP1472238A1 (en) 2004-11-03
EP1472238B1 (en) 2012-07-04
EP2388254B1 (en) 2016-08-10
US20060183177A1 (en) 2006-08-17
US20100249427A1 (en) 2010-09-30
JP2005530485A (ja) 2005-10-13
CA2474695A1 (en) 2003-08-14
US7148030B2 (en) 2006-12-12
JP4451663B2 (ja) 2010-04-14
AU2003216139A1 (en) 2003-09-02
US20030211560A1 (en) 2003-11-13
EP2388254A1 (en) 2011-11-23

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Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired