AU2002317958B2 - Reagents and a method for saturation labelling of proteins - Google Patents

Reagents and a method for saturation labelling of proteins Download PDF

Info

Publication number
AU2002317958B2
AU2002317958B2 AU2002317958A AU2002317958A AU2002317958B2 AU 2002317958 B2 AU2002317958 B2 AU 2002317958B2 AU 2002317958 A AU2002317958 A AU 2002317958A AU 2002317958 A AU2002317958 A AU 2002317958A AU 2002317958 B2 AU2002317958 B2 AU 2002317958B2
Authority
AU
Australia
Prior art keywords
dye
dihydro
group
dimethyl
ethyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU2002317958A
Other languages
English (en)
Other versions
AU2002317958A1 (en
Inventor
Susan Janet Fowler
Adrian Christopher Simmonds
Timothy Stone
Alison Claire Sweet
Karen Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions Germany GmbH
Original Assignee
Global Life Sciences Solutions Germany GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Global Life Sciences Solutions Germany GmbH filed Critical Global Life Sciences Solutions Germany GmbH
Publication of AU2002317958A1 publication Critical patent/AU2002317958A1/en
Assigned to GE HEALTHCARE UK LIMITED reassignment GE HEALTHCARE UK LIMITED Request for Assignment Assignors: Amersham Bio-Sciences UK Limited
Application granted granted Critical
Publication of AU2002317958B2 publication Critical patent/AU2002317958B2/en
Assigned to GLOBAL LIFE SCIENCES SOLUTIONS GERMANY GMBH reassignment GLOBAL LIFE SCIENCES SOLUTIONS GERMANY GMBH Request for Assignment Assignors: GE HEALTHCARE UK LIMITED
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0033—Blends of pigments; Mixtured crystals; Solid solutions
    • C09B67/0034—Mixtures of two or more pigments or dyes of the same type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Indole Compounds (AREA)
AU2002317958A 2002-07-08 2002-07-08 Reagents and a method for saturation labelling of proteins Expired AU2002317958B2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/GB2002/003142 WO2004005933A1 (en) 2002-07-08 2002-07-08 Reagents and a method for saturation labelling of proteins

Publications (2)

Publication Number Publication Date
AU2002317958A1 AU2002317958A1 (en) 2004-01-23
AU2002317958B2 true AU2002317958B2 (en) 2007-07-12

Family

ID=30011660

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002317958A Expired AU2002317958B2 (en) 2002-07-08 2002-07-08 Reagents and a method for saturation labelling of proteins

Country Status (10)

Country Link
US (1) US7511155B2 (https=)
EP (1) EP1520176B1 (https=)
JP (1) JP4318180B2 (https=)
CN (1) CN1307263C (https=)
AT (1) ATE460670T1 (https=)
AU (1) AU2002317958B2 (https=)
CA (1) CA2491692C (https=)
DE (1) DE60235652D1 (https=)
ES (1) ES2341946T3 (https=)
WO (1) WO2004005933A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2589750A1 (en) * 2004-12-06 2006-06-15 National Institute Of Advanced Industrial Science And Technology Method of separating protein, method of staining protein and liquid protein-staining agent and protein-staining kit to be used in these methods
WO2010010158A1 (en) 2008-07-23 2010-01-28 Leibniz-Institut Für Pflanzenbiochemie (Ipb) Qualitative and/or quantitative determination of a proteinaceous molecule in a plurality of samples
US20110226622A1 (en) * 2008-12-02 2011-09-22 Japan Science And Technology Agency Novel clear native electrophoresis method utilizing aromatic sulfonic acid compound
US8729276B2 (en) 2009-04-17 2014-05-20 Korea Institute Of Science And Technology Cyanine compound for labeling biomolecule and preparation method thereof
CN103282509A (zh) * 2010-12-28 2013-09-04 中外制药株式会社 动物细胞的培养方法
CN103113283A (zh) * 2013-01-22 2013-05-22 天津理工大学 一种含给电子基团半菁蓝素温敏分子光学探针的制备方法
CN103113282A (zh) * 2013-01-22 2013-05-22 天津理工大学 一种含吸电子基团半菁蓝素温敏分子光学探针的制备方法
DE102022131451A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
DE102022131445A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131444A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren zum Identifizieren von Analyten in einer Bildfolge
DE102022131446A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131449A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131448A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131450A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131447A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102023122213A1 (de) * 2023-08-18 2025-02-20 Carl Zeiss Microscopy Gmbh Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US6127134A (en) * 1995-04-20 2000-10-03 Carnegie Mellon University Difference gel electrophoresis using matched multiple dyes
EA200000408A1 (ru) * 1997-10-10 2000-12-25 Сайтовиэ, Инк. Новые флуоресцентные сигнальные молекулы и их применение, в том числе, в анализах каспаз
EP1038938B1 (en) * 1999-03-25 2004-05-26 Nisshinbo Industries, Inc. Fluorescent group containing carbodiimides, their precursors and methods for their production
US6114350A (en) * 1999-04-19 2000-09-05 Nen Life Science Products, Inc. Cyanine dyes and synthesis methods thereof
CA2423806C (en) * 2000-09-29 2009-12-22 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
JP2003048879A (ja) * 2001-05-31 2003-02-21 Nisshinbo Ind Inc 蛍光性基含有カルボジイミド化合物及び該化合物の製造方法
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use

Also Published As

Publication number Publication date
US20050233462A1 (en) 2005-10-20
WO2004005933A1 (en) 2004-01-15
ES2341946T3 (es) 2010-06-30
CA2491692A1 (en) 2004-01-15
AU2002317958A1 (en) 2004-01-23
JP4318180B2 (ja) 2009-08-19
CA2491692C (en) 2010-12-07
DE60235652D1 (de) 2010-04-22
US7511155B2 (en) 2009-03-31
EP1520176A1 (en) 2005-04-06
JP2005532543A (ja) 2005-10-27
ATE460670T1 (de) 2010-03-15
CN1650168A (zh) 2005-08-03
EP1520176B1 (en) 2010-03-10
CN1307263C (zh) 2007-03-28

Similar Documents

Publication Publication Date Title
AU2002317958B2 (en) Reagents and a method for saturation labelling of proteins
EP1678258B1 (en) Cyanine dye labelling reagents
JP5008044B2 (ja) エネルギー伝達アッセイ法および試薬
US6995274B2 (en) Cyanine dyes
US5135717A (en) Tetrabenztriazaporphyrin reagents and kits containing the same
CA1337754C (en) Red-shifted phthalocyanine and tetrabenztriazaporphyrin reagents
EP0977766A1 (en) Glycoconjugated fluorescent labeling reagents
AU2003234038B2 (en) Differential analysis of cell surface proteins on closed membrane structures by labelling with dyes in the presence of an internal standard
JP4098839B2 (ja) インドシアニン化合物
EP2270106A2 (en) Indolium compounds containing fluoro-substituents
Thavornpradit et al. Protein labelling and albumin binding characteristics of the near-IR Cy7 fluorophore, QuatCy
US20060141531A1 (en) Ultra-high specificity fluorescent labeling
CN102807588B (zh) 一种原位检测生物体内相邻巯基蛋白质的化合物及其方法
JP2005534739A (ja) シアニン染料リポーターを用いたタンパク質の部位特異的標識
HK1093520B (en) Cyanine dye labelling reagents

Legal Events

Date Code Title Description
TC Change of applicant's name (sec. 104)

Owner name: GE HEALTHCARE UK LIMITED

Free format text: FORMER NAME: AMERSHAM BIO-SCIENCES UK LIMITED

FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: GLOBAL LIFE SCIENCES SOLUTIONS GERMANY GMBH

Free format text: FORMER OWNER(S): GE HEALTHCARE UK LIMITED

MK14 Patent ceased section 143(a) (annual fees not paid) or expired