AU2002227866A1 - Method for quantifying cytosine methylations in genomic dna that is amplified in a complex manner - Google Patents

Method for quantifying cytosine methylations in genomic dna that is amplified in a complex manner

Info

Publication number
AU2002227866A1
AU2002227866A1 AU2002227866A AU2786602A AU2002227866A1 AU 2002227866 A1 AU2002227866 A1 AU 2002227866A1 AU 2002227866 A AU2002227866 A AU 2002227866A AU 2786602 A AU2786602 A AU 2786602A AU 2002227866 A1 AU2002227866 A1 AU 2002227866A1
Authority
AU
Australia
Prior art keywords
quantifying
amplified
genomic dna
complex manner
cytosine methylations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002227866A
Other languages
English (en)
Inventor
Alexander Olek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of AU2002227866A1 publication Critical patent/AU2002227866A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
AU2002227866A 2000-12-06 2001-12-05 Method for quantifying cytosine methylations in genomic dna that is amplified in a complex manner Abandoned AU2002227866A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10061348.9 2000-12-06
DE10061348A DE10061348C2 (de) 2000-12-06 2000-12-06 Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA
PCT/DE2001/004617 WO2002046452A2 (de) 2000-12-06 2001-12-05 Verfahren zur quantifizierung von cytosin-methylierungen in komplex amplifizierter genomischer dna

Publications (1)

Publication Number Publication Date
AU2002227866A1 true AU2002227866A1 (en) 2002-06-18

Family

ID=7666462

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002227866A Abandoned AU2002227866A1 (en) 2000-12-06 2001-12-05 Method for quantifying cytosine methylations in genomic dna that is amplified in a complex manner

Country Status (5)

Country Link
US (1) US20050019762A1 (de)
EP (1) EP1366190A2 (de)
AU (1) AU2002227866A1 (de)
DE (1) DE10061348C2 (de)
WO (1) WO2002046452A2 (de)

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CA2507189C (en) * 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
US20060024676A1 (en) * 2003-04-14 2006-02-02 Karen Uhlmann Method of detecting epigenetic biomarkers by quantitative methyISNP analysis
US20050009053A1 (en) * 2003-04-25 2005-01-13 Sebastian Boecker Fragmentation-based methods and systems for de novo sequencing
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
US7799525B2 (en) 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
WO2005024053A1 (en) * 2003-09-04 2005-03-17 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US9394565B2 (en) * 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US7608394B2 (en) * 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
WO2005098050A2 (en) 2004-03-26 2005-10-20 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US20060019270A1 (en) * 2004-04-01 2006-01-26 Board Of Regents The University Of Texas System Global DNA methylation assessment using bisulfite PCR
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2006026828A1 (en) 2004-09-10 2006-03-16 Human Genetic Signatures Pty Ltd Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn)
US20060073501A1 (en) * 2004-09-10 2006-04-06 Van Den Boom Dirk J Methods for long-range sequence analysis of nucleic acids
NZ555620A (en) * 2004-12-03 2008-08-29 Human Genetic Signatures Pty Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US20090029346A1 (en) * 2004-12-23 2009-01-29 Human Genetic Signatures Pty., Ltd. Detection of human papilloma virus
US7449297B2 (en) 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
AU2006251866B2 (en) * 2005-05-26 2007-11-29 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US8343738B2 (en) * 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
US20070122818A1 (en) * 2005-10-31 2007-05-31 Abhijit Mazumder Gene methylation assay controls
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法
WO2008091913A2 (en) 2007-01-23 2008-07-31 Sequenom, Inc. Method for accurate assessment of dna quality after bisulfite treatment
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
WO2008113111A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
EP2215250B1 (de) 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzyme zur amplifikation und zum kopieren bisulphit-modifizierter nukleinsäuren
US20110003700A1 (en) * 2007-12-20 2011-01-06 Human Genetic Signatures Pty Ltd. Elimination of contaminants associated with nucleic acid amplification
EP2271772B1 (de) * 2008-03-11 2014-07-16 Sequenom, Inc. Tests auf nukleinsäurebasis zur pränatalen geschlechtsbestimmung
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) * 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
WO2011087760A2 (en) 2009-12-22 2011-07-21 Sequenom, Inc. Processes and kits for identifying aneuploidy
CN102533944B (zh) * 2010-12-10 2014-04-09 深圳华大基因科技服务有限公司 用于甲基化dna的富集和测序的半甲基化接头及其用途
EP3378954B1 (de) 2011-04-29 2021-02-17 Sequenom, Inc. Quantifizierung einer minderheitsvariante einer nukleinsäure
CN103874766B (zh) 2011-09-07 2017-07-18 人类遗传标记控股有限公司 分子检测测定
EP4155401A1 (de) 2012-03-02 2023-03-29 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
CA2878979C (en) 2012-07-13 2021-09-14 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2014168711A1 (en) 2013-03-13 2014-10-16 Sequenom, Inc. Primers for dna methylation analysis
EP3117011B1 (de) 2014-03-13 2020-05-06 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
DE19853398C1 (de) * 1998-11-19 2000-03-16 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischer DNA
DE19905082C1 (de) * 1999-01-29 2000-05-18 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben
DE19935772C2 (de) * 1999-07-26 2002-11-07 Epigenomics Ag Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben
ES2299520T3 (es) * 2000-09-01 2008-06-01 Epigenomics Ag Procedimiento para la determinacion del grado de metilacion de determinadas citosinas en dna genomico en el contexto secuencial 5'-cpg-3'.
DE10050942B4 (de) * 2000-10-10 2005-11-17 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen

Also Published As

Publication number Publication date
US20050019762A1 (en) 2005-01-27
DE10061348A1 (de) 2002-06-20
WO2002046452A2 (de) 2002-06-13
WO2002046452A3 (de) 2003-09-18
EP1366190A2 (de) 2003-12-03
DE10061348C2 (de) 2002-10-24

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