AU2002221072A1 - Well unit for detecting cell chemotaxis and separating chemotactic cells - Google Patents

Well unit for detecting cell chemotaxis and separating chemotactic cells

Info

Publication number
AU2002221072A1
AU2002221072A1 AU2002221072A AU2107202A AU2002221072A1 AU 2002221072 A1 AU2002221072 A1 AU 2002221072A1 AU 2002221072 A AU2002221072 A AU 2002221072A AU 2107202 A AU2107202 A AU 2107202A AU 2002221072 A1 AU2002221072 A1 AU 2002221072A1
Authority
AU
Australia
Prior art keywords
cells
well unit
chemotaxis
detecting cell
cell chemotaxis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002221072A
Other languages
English (en)
Inventor
Shiro Kanegasaki
Hiroko Kikuchi
Yuji Kikuchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Effector Cell Institute Inc
Original Assignee
Effector Cell Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Effector Cell Institute Inc filed Critical Effector Cell Institute Inc
Publication of AU2002221072A1 publication Critical patent/AU2002221072A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2002221072A 2000-12-07 2001-12-06 Well unit for detecting cell chemotaxis and separating chemotactic cells Abandoned AU2002221072A1 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
JP2000-372467 2000-12-07
JP2000372467 2000-12-07
JP2000377120 2000-12-12
JP2000-377120 2000-12-12
JP2001209743 2001-07-10
JP2001-209743 2001-07-10
JP2001258526 2001-08-28
JP2001-258526 2001-08-28
JP2001313205 2001-10-10
JP2001-313205 2001-10-10
PCT/JP2001/010683 WO2002046355A1 (fr) 2000-12-07 2001-12-06 Unite cupulaire pour detecter la chimiotaxie cellulaire et separer les cellules chimiotactiques

Publications (1)

Publication Number Publication Date
AU2002221072A1 true AU2002221072A1 (en) 2002-06-18

Family

ID=27531737

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002221072A Abandoned AU2002221072A1 (en) 2000-12-07 2001-12-06 Well unit for detecting cell chemotaxis and separating chemotactic cells

Country Status (10)

Country Link
US (1) US7022516B2 (ko)
EP (1) EP1340809B1 (ko)
KR (1) KR100445131B1 (ko)
CN (1) CN1236046C (ko)
AT (1) ATE457198T1 (ko)
AU (1) AU2002221072A1 (ko)
CA (1) CA2400738A1 (ko)
DE (1) DE60141287D1 (ko)
TW (1) TWI241343B (ko)
WO (1) WO2002046355A1 (ko)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3738899B2 (ja) * 2000-12-07 2006-01-25 株式会社 エフェクター細胞研究所 微量試料処理装置
AU2002366983A1 (en) * 2001-12-31 2003-07-30 The Provost Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Assembly for cell-based assays
WO2004020573A1 (en) * 2002-08-27 2004-03-11 Vanderbilt University Bioreactors with an array of chambers and a common feed line
US20070177255A1 (en) * 2003-03-27 2007-08-02 Shiro Kanegasaki Observing tool and observing method using the same
JP4434649B2 (ja) * 2003-03-27 2010-03-17 株式会社Ｅｃｉ 観察器具及びそれを用いた観察方法
TW200506364A (en) * 2003-04-09 2005-02-16 Effector Cell Inst Inc Apparatus for detecting cell chemo-taxis
CA2522410A1 (en) * 2003-05-19 2004-11-25 Japan Science And Technology Agency Micro chamber for cell culture
KR100573621B1 (ko) * 2003-07-18 2006-04-25 주식회사 디지탈바이오테크놀러지 세포 개체수 계수용 장치 및 그 제조방법
JP2006006166A (ja) * 2004-06-24 2006-01-12 Univ Of Tokyo 細胞分離装置、及び細胞分離方法
US20080257735A1 (en) * 2004-09-24 2008-10-23 Regents Of The University Of California Microfluidic Device for Enabling the Controlled Growth of Cells and Methods Relating to Same
US7276367B2 (en) * 2004-10-14 2007-10-02 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Method of etching islands of cells in a grid pattern
CN101036043B (zh) * 2004-12-07 2010-10-13 株式会社Eci 细胞计测方法
US9354156B2 (en) * 2007-02-08 2016-05-31 Emd Millipore Corporation Microfluidic particle analysis method, device and system
EP1764409A1 (en) * 2005-09-12 2007-03-21 Institut National De La Sante Et De La Recherche Medicale (Inserm) Device and method for observing the organization of studied cells cultured in the presence of a concentration gradient of chemotactic molecules
US20070084706A1 (en) * 2005-10-18 2007-04-19 Shuichi Takayama Microfluidic cell culture device and method for using same
US20070090166A1 (en) 2005-10-18 2007-04-26 Shuichi Takayama Microfluidic cell culture device
US20100247384A1 (en) * 2005-10-18 2010-09-30 Shuichi Takayama Microfluidic cell culture device and method for using same
US8603806B2 (en) * 2005-11-02 2013-12-10 The Ohio State Universtiy Research Foundation Materials and methods for cell-based assays
KR100848811B1 (ko) 2006-09-29 2008-07-28 전자부품연구원 세포 배양 환경 및 주화성 측정 장치
CN101715429B (zh) * 2007-05-15 2012-05-23 和光纯药工业株式会社 均衡集成式pcr-ce微流装置内的压力的压力歧管
EP2163880A1 (en) * 2008-09-10 2010-03-17 Koninklijke Philips Electronics N.V. Method and use for the separation of biological material from a sample fluid
FR2937050A1 (fr) * 2008-10-10 2010-04-16 Inst Curie Dispositif de culture cellulaire
US9157550B2 (en) * 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
EP2599548B1 (de) * 2009-05-13 2018-07-04 ibidi GmbH Probenträger zum Positionieren einer organischen, biologischen und/oder medizinischen Probe
US8778669B2 (en) 2009-07-22 2014-07-15 Corning Incorporated Multilayer tissue culture vessel
KR101126547B1 (ko) 2009-12-01 2012-03-22 한국생산기술연구원 세포 주화성 검사용 마이크로 플루이딕 칩 및 제조방법
KR101160188B1 (ko) * 2010-01-14 2012-06-26 김영호 자력을 이용한 세포분리기
US10526572B2 (en) 2011-04-01 2020-01-07 EMD Millipore Corporaticn Cell culture and invasion assay method and system
CN103998394B (zh) * 2011-08-01 2016-08-17 德诺弗科学公司 细胞捕获系统和使用方法
CN102925356A (zh) * 2011-08-09 2013-02-13 华中科技大学同济医学院附属协和医院 一种细胞培养池
WO2015009688A1 (en) * 2013-07-17 2015-01-22 The Johns Hopkins University Microfluidic chip for analysis of cell motility and methods for using same
US10077420B2 (en) * 2014-12-02 2018-09-18 Histogenics Corporation Cell and tissue culture container
CN104792750B (zh) * 2015-03-27 2017-11-28 南方医科大学 细胞极性模型的构建方法
US20160354781A1 (en) * 2015-06-08 2016-12-08 National Taiwan University Microfluidic plate for sample processing
CN107226453B (zh) * 2016-03-24 2021-08-13 中芯国际集成电路制造(上海)有限公司 一种mems器件及其制备方法、电子装置
KR101895776B1 (ko) * 2016-04-07 2018-09-07 영남대학교 산학협력단 실리콘 마이크로칩 및 그 제조방법
JP7006600B2 (ja) * 2016-08-23 2022-02-10 ソニーグループ株式会社 単一粒子捕捉用装置、単一粒子捕捉システム及び単一粒子の捕捉方法
KR102088102B1 (ko) * 2017-07-05 2020-03-11 가부시키가이샤 스크린 홀딩스 시료 용기
CN109706076A (zh) * 2019-01-14 2019-05-03 上海市肿瘤研究所 观察细胞迁移极化分子分布的方法
CN113899659A (zh) * 2020-06-22 2022-01-07 苏州中加康美科技有限公司 一种载玻片及血细胞分析仪
CN114164078A (zh) * 2021-11-30 2022-03-11 齐齐哈尔大学 一种细菌趋化物质筛选载玻片及其应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3888770A (en) 1971-10-21 1975-06-10 Shlomo Avital Plural-sample filter device
US3929583A (en) 1975-08-14 1975-12-30 Canadian Patents Dev Apparatus for enumerating microorganisms
US4317726A (en) 1981-02-12 1982-03-02 The United States Of America As Represented By The Secretary Of The Army Microbial filter assembly
IL68507A (en) 1982-05-10 1986-01-31 Univ Bar Ilan System and methods for cell selection
US4514495A (en) 1982-05-18 1985-04-30 Spiral Systems Instruments, Inc. Method for testing microbial interaction with growth affecting substances
US4493815A (en) 1983-07-28 1985-01-15 Bio-Rad Laboratories, Inc. Supporting and filtering biochemical test plate assembly
US4714674A (en) 1985-02-28 1987-12-22 Genentech, Inc. Chemotactic assay for immunogenicity
US4833382A (en) * 1986-06-06 1989-05-23 Gibbs David L Method and apparatus for use in microscope investigations
US5023173A (en) 1986-10-14 1991-06-11 Xoma Corporation Device for assessing nematode vitality and method for using same
JP2559760B2 (ja) 1987-08-31 1996-12-04 株式会社日立製作所 細胞搬送方法
JP2685544B2 (ja) * 1988-11-11 1997-12-03 株式会社日立製作所 血液フィルタおよび血液検査方法並びに血液検査装置
US4912057A (en) 1989-06-13 1990-03-27 Cancer Diagnostics, Inc. Cell chamber for chemotaxis assay
JP2532707B2 (ja) * 1990-03-08 1996-09-11 佑二 菊池 血液回路及びこれを用いた血液測定装置及び血液測定方法
US5284753A (en) * 1991-03-20 1994-02-08 Neuro Probe, Inc. Multiple-site chemotactic test apparatus and method
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5302515A (en) 1992-08-20 1994-04-12 Neuro Probe, Inc. Chemotactic test apparatus and method
JP2685119B2 (ja) * 1994-07-15 1997-12-03 浜松ホトニクス株式会社 細胞分画方法及び細胞分画装置
US5595712A (en) 1994-07-25 1997-01-21 E. I. Du Pont De Nemours And Company Chemical mixing and reaction apparatus
JP3089285B2 (ja) 1997-12-02 2000-09-18 農林水産省食品総合研究所長 積層マイクロチャネルアレイ装置並びに同装置を用いた濾過・分級方法及びエマルションの製造方法
WO2000007007A1 (en) * 1998-07-28 2000-02-10 Biometric Imaging, Inc. Device and method for cell motility assay
US6329164B1 (en) * 1999-03-18 2001-12-11 Neuro Probe, Incorporated Method for using a cell activity assay apparatus
GB9925904D0 (en) 1999-11-03 1999-12-29 Univ Belfast Cell migration and chemotaxis chamber
JP2002159287A (ja) * 2000-09-12 2002-06-04 Effector Cell Institute Inc 細胞走化性検出及び走化細胞分離装置
WO2002042766A2 (en) 2000-10-26 2002-05-30 University Of Connecticut A system and method for investigating the effect of chemical and other factors on cell movement
JP3738899B2 (ja) * 2000-12-07 2006-01-25 株式会社 エフェクター細胞研究所 微量試料処理装置

Also Published As

Publication number Publication date
CN1236046C (zh) 2006-01-11
CA2400738A1 (en) 2002-06-13
EP1340809B1 (en) 2010-02-10
US7022516B2 (en) 2006-04-04
KR20020075786A (ko) 2002-10-05
DE60141287D1 (de) 2010-03-25
TWI241343B (en) 2005-10-11
CN1398296A (zh) 2003-02-19
EP1340809A1 (en) 2003-09-03
WO2002046355A1 (fr) 2002-06-13
US20030003571A1 (en) 2003-01-02
ATE457198T1 (de) 2010-02-15
KR100445131B1 (ko) 2004-08-21
EP1340809A4 (en) 2007-04-04

Similar Documents

Publication Publication Date Title
AU2002221072A1 (en) Well unit for detecting cell chemotaxis and separating chemotactic cells
AU2001250883A1 (en) Method for detecting an analyte by fluorescence
WO2003023360A3 (en) Methods and apparatus for conducting multiple measurements on a sample
AU2003201232A1 (en) Faims with non-destructive detection of selectively transmitted ions
GB2430740A (en) System and method for tracking and quatitating chemical entites
DK1483527T3 (da) Segment til et sensorbærende element i en rørrenser
WO2009057793A1 (ja) 分析用具、分析装置、試料不足の検知方法および試料分析方法
WO2005122296A3 (en) An electrode device, process for making an electrode device and a method of electrochemical detection using an electrode device
WO2007002132A3 (en) High frequency or multifrequency resistivity tool
ATE510214T1 (de) Krankheitsdiagnose
GB2411281B (en) Storage device
CN202793914U (zh) 微型性能检测拉伸夹具
DE60107411D1 (de) Elektrochemisches messgerät
WO2003081253A3 (en) Electrochemical multi-well plate
WO2006029302A3 (en) Dioxetane-nanoparticle assemblies for energy transfer detection systems, methods of making the assemblies, and methods of using the assemblies in bioassays
FI974003A (fi) Paperikoneen tai paperin jälkikäsittelykoneen nipin läpi kulkevan pinnan likaantumisen ja/tai vaurioitumisen havaitsemismenetelmä
WO2007026232A3 (en) Methods and apparatus for detecting liquid inside individual channels in a multi-channel plate
EP1965211A4 (en) PROCESS FOR DETECTING SUGAR CHAIN HAVING GnT-V-TRANSFERRED GlcNAc
SG148027A1 (en) Methods for identifying combinations of entities as therapeutics
WO2004055517A3 (en) Nucleophosmin directed diagnostics and therapeutics for multidrug resistant neoplastic disease
TW200508686A (en) Serial routing of optical signals
WO2004008566A3 (de) Vorrichtung und zur prüfung einer elektroden-membran-einheit
WO2003020886A3 (en) Methods and devices for detecting cell-cell interactions
WO2001059446A3 (en) Assay for detecting change in membrane potential
NO20073810L (no) Sensorbrikke