AU2002220870A1 - Process for the preparation of phosphorothionate oligonucleotides - Google Patents

Process for the preparation of phosphorothionate oligonucleotides

Info

Publication number
AU2002220870A1
AU2002220870A1 AU2002220870A AU2087002A AU2002220870A1 AU 2002220870 A1 AU2002220870 A1 AU 2002220870A1 AU 2002220870 A AU2002220870 A AU 2002220870A AU 2087002 A AU2087002 A AU 2087002A AU 2002220870 A1 AU2002220870 A1 AU 2002220870A1
Authority
AU
Australia
Prior art keywords
oligonucleotide
solid support
process according
acetonitrile
solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002220870A
Other languages
English (en)
Inventor
Mark Edward Douglas
Ben James Mellor
Donald Alfred Wellings
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Avecia Ltd
Original Assignee
Avecia Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0029610A external-priority patent/GB0029610D0/en
Priority claimed from US09/740,031 external-priority patent/US6768005B2/en
Application filed by Avecia Ltd filed Critical Avecia Ltd
Publication of AU2002220870A1 publication Critical patent/AU2002220870A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
AU2002220870A 2000-12-05 2001-12-03 Process for the preparation of phosphorothionate oligonucleotides Abandoned AU2002220870A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0029610.3 2000-12-05
GB0029610A GB0029610D0 (en) 2000-12-05 2000-12-05 Process
US09/740,031 2000-12-20
US09/740,031 US6768005B2 (en) 2000-12-20 2000-12-20 Process
PCT/GB2001/005338 WO2002046205A2 (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothionate oligonucleotides

Publications (1)

Publication Number Publication Date
AU2002220870A1 true AU2002220870A1 (en) 2002-06-18

Family

ID=26245377

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002220870A Abandoned AU2002220870A1 (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothionate oligonucleotides

Country Status (8)

Country Link
US (1) US7227017B2 (de)
EP (2) EP1343802B1 (de)
JP (1) JP2004517089A (de)
KR (1) KR20040016826A (de)
CN (1) CN1302005C (de)
AU (1) AU2002220870A1 (de)
CA (2) CA2437040C (de)
WO (1) WO2002046205A2 (de)

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GB0229443D0 (en) 2002-12-18 2003-01-22 Avecia Ltd Process
EP1660512A4 (de) * 2003-06-02 2009-12-23 Isis Pharmaceuticals Inc Oligonucleotid-synthese mit alternativen lösungsmitteln
EP2205619A4 (de) * 2007-10-15 2013-04-17 Honeywell Int Inc Synthese von oligonukleotiden oder phosphorothioatoligonukleotid mit verkappungsmittel aus 1,3,5-trimethylhexahydro-1,3,5-trazin-freiem n-methylimidazol
WO2012059510A1 (en) * 2010-11-02 2012-05-10 Girindus America, Inc. Back pressure control during solid-phase synthesis on polymeric supports
US9403865B2 (en) * 2012-08-15 2016-08-02 Ionis Pharmaceuticals, Inc. Method of preparing oligomeric compounds using modified capping protocols
GB2533173A (en) 2013-08-05 2016-06-15 Twist Bioscience Corp De Novo synthesized gene libraries
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EA201890763A1 (ru) 2015-09-18 2018-08-31 Твист Байосайенс Корпорейшн Библиотеки вариантных олигонуклеиновых кислот и их синтез
KR20180058772A (ko) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
CN110248724B (zh) 2016-09-21 2022-11-18 特韦斯特生物科学公司 基于核酸的数据存储
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
GB2578844A (en) 2017-06-12 2020-05-27 Twist Bioscience Corp Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CA3075505A1 (en) 2017-09-11 2019-03-14 Twist Bioscience Corporation Gpcr binding proteins and synthesis thereof
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物
JP7191448B2 (ja) 2018-01-04 2022-12-19 ツイスト バイオサイエンス コーポレーション Ｄｎａベースのデジタル情報ストレージ
KR20210013128A (ko) 2018-05-18 2021-02-03 트위스트 바이오사이언스 코포레이션 핵산 하이브리드화를 위한 폴리뉴클레오타이드, 시약 및 방법
CN113766930A (zh) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Glp1受体的变异核酸文库
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
CN114573652A (zh) * 2022-03-15 2022-06-03 通用生物(滁州)有限公司 药用核酸合成工艺

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US5132418A (en) * 1980-02-29 1992-07-21 University Patents, Inc. Process for preparing polynucleotides
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US4973679A (en) * 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
CA1206457A (en) * 1981-10-07 1986-06-24 Alan Rosevear Synthesis of compounds
USRE34069E (en) * 1983-08-18 1992-09-15 Biosyntech Gmbh Process for the preparation of oligonucleotides
US5362866A (en) 1983-09-02 1994-11-08 Molecular Biosystems, Inc. Oligonucleotide polymeric support system with an oxidation cleavable link
US4795700A (en) 1985-01-25 1989-01-03 California Institute Of Technology Nucleic acid probes and methods of using same
EP0216357A3 (de) * 1985-09-25 1988-08-31 Nippon Zeon Co., Ltd. Phosphoramidit-Verbindungen und Verfahren zu deren Herstellung
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
CA1315968C (en) * 1987-04-24 1993-04-13 David Colin Sherrington Substrate and process for making a substrate
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0291088A (ja) * 1988-09-29 1990-03-30 Central Glass Co Ltd ホスファイトおよびヌクレオシド‐３’‐ホスファイト誘導体およびこれを用いるオリゴヌクレオチドの合成法
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SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
CA2124796A1 (en) 1991-12-23 1993-07-08 Chiron Diagnostics Corporation Cmv probes for use in solution phase sandwich hybridization assays
WO1994001446A2 (en) * 1992-07-09 1994-01-20 Beckman Instruments, Inc. Derivatized organic solid support for nucleic acid synthesis
US5614621A (en) * 1993-07-29 1997-03-25 Isis Pharmaceuticals, Inc. Process for preparing oligonucleotides using silyl-containing diamino phosphorous reagents
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
US5554746A (en) * 1994-05-16 1996-09-10 Isis Pharmaceuticals, Inc. Lactam nucleic acids
US5510476A (en) * 1994-07-07 1996-04-23 Isis Pharmaceuticals, Inc. Carbocation scavenging during oligonucleotide synthesis
US5705621A (en) * 1995-11-17 1998-01-06 Isis Pharmaceuticals, Inc. Oligomeric phosphite, phosphodiester, Phosphorothioate and phosphorodithioate compounds and intermediates for preparing same
US5869696A (en) * 1996-04-22 1999-02-09 Beckman Instruments, Inc. Universal solid supports and methods for their use
US6096881A (en) * 1997-05-30 2000-08-01 Hybridon, Inc. Sulfur transfer reagents for oligonucleotide synthesis
US6111086A (en) * 1998-02-27 2000-08-29 Scaringe; Stephen A. Orthoester protecting groups
US6069243A (en) * 1998-10-06 2000-05-30 Isis Pharmaceuticals, Inc. Process for oligonucleotide synthesis
US6465628B1 (en) 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
JP4705716B2 (ja) 1999-02-05 2011-06-22 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション オリゴヌクレオチドの脱保護法
GB9924285D0 (en) * 1999-10-14 1999-12-15 Avecia Ltd Process
JP2004503561A (ja) * 2000-06-12 2004-02-05 アベシア・バイオテクノロジー・インコーポレーテッド 合成ヌクレオチドの修飾を防ぐ方法
US6768005B2 (en) * 2000-12-20 2004-07-27 Avecia Limited Process
JP4348044B2 (ja) * 2002-02-12 2009-10-21 株式会社キラルジェン 立体規則性の高いジヌクレオシドホスホロチオエートの製造法
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process

Also Published As

Publication number Publication date
CA2437040C (en) 2011-01-25
CN1302005C (zh) 2007-02-28
EP2332954A2 (de) 2011-06-15
US7227017B2 (en) 2007-06-05
EP1343802A2 (de) 2003-09-17
CA2714353A1 (en) 2002-06-13
EP1343802B1 (de) 2012-05-30
WO2002046205A2 (en) 2002-06-13
CN1479745A (zh) 2004-03-03
KR20040016826A (ko) 2004-02-25
JP2004517089A (ja) 2004-06-10
WO2002046205A3 (en) 2002-08-15
EP2332954A3 (de) 2011-12-21
US20030229221A1 (en) 2003-12-11
CA2437040A1 (en) 2002-06-13

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application