AU2002215010A1 - Thermostable polymerase based on thermococcus pacificus - Google Patents

Thermostable polymerase based on thermococcus pacificus

Info

Publication number
AU2002215010A1
AU2002215010A1 AU2002215010A AU1501002A AU2002215010A1 AU 2002215010 A1 AU2002215010 A1 AU 2002215010A1 AU 2002215010 A AU2002215010 A AU 2002215010A AU 1501002 A AU1501002 A AU 1501002A AU 2002215010 A1 AU2002215010 A1 AU 2002215010A1
Authority
AU
Australia
Prior art keywords
polymerase
thermostable polymerase
polymerase based
thermococcus pacificus
pacificus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002215010A
Other languages
English (en)
Inventor
Katja Decker
Jie Kang
Dirk Loffert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of AU2002215010A1 publication Critical patent/AU2002215010A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
  • Saccharide Compounds (AREA)
AU2002215010A 2000-10-05 2001-10-05 Thermostable polymerase based on thermococcus pacificus Abandoned AU2002215010A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10049211 2000-10-05
DE10049211A DE10049211A1 (de) 2000-10-05 2000-10-05 Thermostabile Polymerase aus Thermococcus pacificus
PCT/EP2001/011529 WO2002029016A2 (fr) 2000-10-05 2001-10-05 Polymerase thermostable a base de thermococcus pacificus

Publications (1)

Publication Number Publication Date
AU2002215010A1 true AU2002215010A1 (en) 2002-04-15

Family

ID=7658701

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002215010A Abandoned AU2002215010A1 (en) 2000-10-05 2001-10-05 Thermostable polymerase based on thermococcus pacificus

Country Status (6)

Country Link
US (1) US8124391B2 (fr)
EP (1) EP1326964B1 (fr)
AT (1) ATE496122T1 (fr)
AU (1) AU2002215010A1 (fr)
DE (2) DE10049211A1 (fr)
WO (1) WO2002029016A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60133992D1 (de) * 2000-02-17 2008-06-26 Qiagen Gmbh Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
DE10049211A1 (de) 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
EP1456409B1 (fr) * 2001-11-28 2010-02-24 Bio-Rad Laboratories, Inc. Determination de polypmorphisme parallele par amplification et correction d'erreur
AU2005230833B2 (en) 2004-04-01 2011-04-14 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
CN112029744A (zh) * 2020-08-26 2020-12-04 上海交通大学 Dna聚合酶及其编码基因、制备方法和pcr应用
TW202330915A (zh) * 2021-09-29 2023-08-01 呈堯 陳 用於無模板ｒｎａ合成的核酸聚合酶變體、試劑套組及方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5242818A (en) * 1988-08-26 1993-09-07 Toyo Boseki Kabushiki Kaisha Method of producing a thermostable DNA polymerase from Thermus thermophilus
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5210036A (en) * 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
DE69131321T2 (de) 1990-09-28 1999-12-16 F. Hoffmann-La Roche Ag, Basel Mutationen in der 5'-3'-exonukleaseaktivität thermostabiler dna-polymerasen
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5491086A (en) 1993-05-14 1996-02-13 Hoffmann-La Roche Inc. Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
CN1123328A (zh) 1994-11-17 1996-05-29 中国科学院上海生物化学研究所 高温、高传真脱氧核糖核酸(DNA)聚合酶(HiFi Bst)
US5602011A (en) 1995-01-18 1997-02-11 Pharmacia Biotech Inc. Purified Thermococcus barossii DNA polymerase
JP3112148B2 (ja) 1995-05-31 2000-11-27 東洋紡績株式会社 核酸の増幅方法およびその試薬
US5972603A (en) 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
JP4217922B2 (ja) 1996-03-19 2009-02-04 東洋紡績株式会社 サーモコッカス・ペプトノフィラス由来の熱安定性ｄｎａポリメラーゼ、該酵素をコードする遺伝子およびその用途
DE69725076T2 (de) * 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
EP0834571A1 (fr) * 1996-10-03 1998-04-08 Roche Diagnostics GmbH Polymérase d'ADN thermostable de Thermococcus Gorgonarius
EP0834570A1 (fr) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Polymérase d'ADN thermostable de thermostable gorgonarius
JP4362150B2 (ja) 1996-11-29 2009-11-11 サード ウェーブ テクノロジーズ，インコーポレーテッド Ｆｅｎ−１エンドヌクレアーゼ、混合物、および開裂方法
WO1998049274A1 (fr) * 1997-04-29 1998-11-05 Appligene-Oncor Adn polymerase thermostable et inteines de l'espece thermococcus fumicolans
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
GB9818432D0 (en) 1998-08-24 1998-10-21 Bioline Limited Thermostable DNA Polymerase
GB9921318D0 (en) 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US20030228616A1 (en) 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
DE60133992D1 (de) * 2000-02-17 2008-06-26 Qiagen Gmbh Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
DE10049211A1 (de) 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus

Also Published As

Publication number Publication date
ATE496122T1 (de) 2011-02-15
EP1326964A2 (fr) 2003-07-16
DE50115782D1 (de) 2011-03-03
US8124391B2 (en) 2012-02-28
WO2002029016A2 (fr) 2002-04-11
DE10049211A1 (de) 2002-04-18
US20040053279A1 (en) 2004-03-18
WO2002029016A3 (fr) 2002-06-27
EP1326964B1 (fr) 2011-01-19

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