AU2002213175B2 - Solid support assay systems and methods utilizing non-standard bases - Google Patents
Solid support assay systems and methods utilizing non-standard bases Download PDFInfo
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- AU2002213175B2 AU2002213175B2 AU2002213175A AU2002213175A AU2002213175B2 AU 2002213175 B2 AU2002213175 B2 AU 2002213175B2 AU 2002213175 A AU2002213175 A AU 2002213175A AU 2002213175 A AU2002213175 A AU 2002213175A AU 2002213175 B2 AU2002213175 B2 AU 2002213175B2
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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| US29325901P | 2001-05-22 | 2001-05-22 | |
| US60/293,259 | 2001-05-22 | ||
| PCT/US2001/031993 WO2002033126A2 (en) | 2000-10-14 | 2001-10-15 | Solid support assay systems and methods utilizing non-standard bases |
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Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5138141B2 (ja) * | 2000-05-19 | 2013-02-06 | ルミネックス コーポレーション | 核酸の検出用物質及び検出方法 |
| DE60137649D1 (de) * | 2000-10-14 | 2009-03-26 | Eragen Biosciences Inc | Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen |
| US20030194705A1 (en) * | 2002-03-01 | 2003-10-16 | Schroth Gary P. | Methods of using unnatural nucleobases for decoding |
| ATE417129T1 (de) * | 2003-01-17 | 2008-12-15 | Eragen Biosciences Inc | Nukleinsäureamplifikation mit nichtstandardbasen |
| EP1615942B1 (en) * | 2003-04-01 | 2016-03-16 | Luminex Corporation | Polymerase inhibitor and method of using same |
| US7282333B2 (en) * | 2003-10-20 | 2007-10-16 | Promega Corporation | Methods and compositions for nucleic acid analysis |
| WO2005081776A2 (en) * | 2004-01-30 | 2005-09-09 | Eragen Biosciences, Inc. | Materials and methods for the detection of sars |
| WO2006101913A2 (en) * | 2005-03-18 | 2006-09-28 | Eragen Biosciences, Inc. | Methods for detecting multiple species and subspecies of neiserria |
| US8293472B2 (en) | 2005-06-07 | 2012-10-23 | Luminex Corporation | Methods for detection and typing of nucleic acids |
| US7759062B2 (en) * | 2006-06-09 | 2010-07-20 | Third Wave Technologies, Inc. | T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection |
| US7794936B1 (en) * | 2006-09-29 | 2010-09-14 | Steven Albert Benner | Using thymidine analogs to improve replication in an expanded genetic alphabet |
| US20080153097A1 (en) * | 2006-11-15 | 2008-06-26 | Eragen Biosciences, Inc. | Methods and kits for detecting jak2 nucleic acid |
| US9725713B1 (en) * | 2007-01-22 | 2017-08-08 | Steven A Benner | In vitro selection with expanded genetic alphabets |
| US20080299568A1 (en) * | 2007-04-27 | 2008-12-04 | Scott Johnson | Materials and methods for detection of hepatitis c virus |
| US20100086908A1 (en) * | 2007-05-17 | 2010-04-08 | Prudent James R | Methods for the detection of respiratory viruses |
| US9249455B2 (en) * | 2008-04-18 | 2016-02-02 | Luminex Corporation | Methods for detection and quantification of small RNA |
| EP2133434A1 (en) | 2008-06-12 | 2009-12-16 | Institut National de la Santé et de la Recherche Médicale (INSERM) | Method for the detection of target nucleic acid |
| US10865431B1 (en) * | 2008-06-17 | 2020-12-15 | Steven A Benner | Polymerase incorporation of non-standard nucleotides |
| WO2009154733A2 (en) * | 2008-06-17 | 2009-12-23 | Steven Albert Benner | Polymerase incorporation of non-standard nucleotides |
| US9637783B1 (en) * | 2008-06-17 | 2017-05-02 | Steven Benner | Amplification of oligonucleotides containing non-standard nucleotides by polymerase chain reactions |
| EP2432796B1 (en) * | 2009-05-21 | 2016-08-17 | Siemens Healthcare Diagnostics Inc. | Universal tags with non-natural nucleobases |
| JPWO2011043385A1 (ja) | 2009-10-06 | 2013-03-04 | 独立行政法人理化学研究所 | 特異な塩基対を形成する人工塩基対 |
| CA2778449C (en) | 2009-10-23 | 2017-06-20 | Luminex Corporation | Amplification primers with non-standard bases for increased reaction specificity |
| WO2013036668A1 (en) | 2011-09-06 | 2013-03-14 | Gen-Probe Incorporated | Circularized templates for sequencing |
| EP4219741A3 (en) | 2011-09-06 | 2023-08-23 | Gen-Probe Incorporated | Closed nucleic acid structures |
| CN104271771A (zh) * | 2012-06-20 | 2015-01-07 | 东丽株式会社 | 核酸的检测方法及核酸检测试剂盒 |
| US20140378327A1 (en) | 2013-06-19 | 2014-12-25 | Luminex Corporation | Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres |
| EP4321867B1 (en) | 2013-08-09 | 2025-10-01 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| US9587268B2 (en) * | 2014-01-29 | 2017-03-07 | Agilent Technologies Inc. | Fast hybridization for next generation sequencing target enrichment |
| US9670088B2 (en) | 2014-05-20 | 2017-06-06 | Corning Incorporated | Scratch resistant glass and method of making |
| WO2015191815A1 (en) | 2014-06-13 | 2015-12-17 | Life Technologies Corporation | Multiplex nucleic acid amplification |
| KR20220155394A (ko) | 2014-08-11 | 2022-11-22 | 루미넥스 코포레이션 | 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브 |
| US20180163259A1 (en) | 2015-05-01 | 2018-06-14 | Gen-Probe Incorporated | Multiplex invasive cleavage assays |
| EP3325666B1 (en) | 2015-07-17 | 2020-04-29 | Luminex Corporation | Methods for catalytic assays |
| WO2017205506A1 (en) | 2016-05-24 | 2017-11-30 | Emory University | Particles with rna cleaving nucleobase polymers and uses for managing inflammatory disorders |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0742287A2 (en) * | 1995-05-10 | 1996-11-13 | McGall, Glenn H. | Modified nucleic acid probes |
| WO1997046711A1 (en) * | 1996-06-06 | 1997-12-11 | Wisconsin Alumni Research Foundation | Artificial mismatch hybridization |
Family Cites Families (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4996143A (en) | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| GB8827160D0 (en) * | 1988-11-21 | 1988-12-29 | Apothekernes Lab | Detection & quantitative determination of rna & dna |
| GB8902689D0 (en) | 1989-02-07 | 1989-03-30 | Ici Plc | Assay method |
| US5856092A (en) * | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| NZ235032A (en) | 1989-08-31 | 1993-04-28 | Dow Chemical Co | Constrained geometry complexes of titanium, zirconium or hafnium comprising a substituted cyclopentadiene ligand; use as olefin polymerisation catalyst component |
| GB8920097D0 (en) | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US6007984A (en) | 1989-11-01 | 1999-12-28 | Zeneca Limited | Detection of DNA/RNA by fluorescence polarization |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5965364A (en) * | 1990-10-09 | 1999-10-12 | Benner; Steven Albert | Method for selecting functional deoxyribonucleotide derivatives |
| US5432272A (en) * | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| US6140496A (en) | 1990-10-09 | 2000-10-31 | Benner; Steven Albert | Precursors for deoxyribonucleotides containing non-standard nucleosides |
| US6037120A (en) * | 1995-10-12 | 2000-03-14 | Benner; Steven Albert | Recognition of oligonucleotides containing non-standard base pairs |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| DE4129653A1 (de) * | 1991-09-06 | 1993-03-11 | Boehringer Mannheim Gmbh | Verfahren zum nachweis aehnlicher nukleinsaeuren |
| US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| AU3933393A (en) * | 1993-03-17 | 1994-10-11 | City Of Hope | Nucleic acid sequences having single stranded ends |
| US6077668A (en) | 1993-04-15 | 2000-06-20 | University Of Rochester | Highly sensitive multimeric nucleic acid probes |
| US5654138A (en) | 1993-05-14 | 1997-08-05 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Von hippel-lindau (VHL) disease gene and corresponding cDNA and methods for detecting carriers of the VHL disease gene |
| US5589329A (en) * | 1993-11-15 | 1996-12-31 | Ambion, Inc. | Methods and compositions for detecting base pair mismatches |
| WO1995021271A1 (en) * | 1994-02-07 | 1995-08-10 | Molecular Tool, Inc. | Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| US5604097A (en) * | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US5866337A (en) * | 1995-03-24 | 1999-02-02 | The Trustees Of Columbia University In The City Of New York | Method to detect mutations in a nucleic acid using a hybridization-ligation procedure |
| GB9507238D0 (en) * | 1995-04-07 | 1995-05-31 | Isis Innovation | Detecting dna sequence variations |
| US5736330A (en) * | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| US5980861A (en) * | 1996-03-12 | 1999-11-09 | University Of Massachusetts | Chelator compositions and methods of synthesis thereof |
| AU2320597A (en) * | 1996-03-19 | 1997-10-10 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
| US6124092A (en) | 1996-10-04 | 2000-09-26 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
| US5853993A (en) | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| JP4185185B2 (ja) | 1997-05-08 | 2008-11-26 | 征夫 軽部 | 部分二重鎖ｄｎａを利用したｄｎａの検出方法 |
| US5928869A (en) | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| JP4502502B2 (ja) | 1997-10-28 | 2010-07-14 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | フローサイトメトリーを用いるｄｎａ多型の同定方法 |
| GB9725197D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| US6046807A (en) * | 1998-05-14 | 2000-04-04 | Luminex Corporation | Diode laser based measurement apparatus |
| EP2360271A1 (en) | 1998-06-24 | 2011-08-24 | Illumina, Inc. | Decoding of array sensors with microspheres |
| CA2357601A1 (en) * | 1998-12-30 | 2000-07-06 | Dana-Farber Cancer Institute, Inc. | Mutation scanning array, and methods of use thereof |
| US6432642B1 (en) | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
| US6528254B1 (en) | 1999-10-29 | 2003-03-04 | Stratagene | Methods for detection of a target nucleic acid sequence |
| JP2001204497A (ja) * | 2000-01-20 | 2001-07-31 | Japan Science & Technology Corp | 蛍光共鳴エネルギー移動を利用した、ｄｎａハイブリダイゼーション法によるｄｎａ検出方法 |
| US6783985B1 (en) * | 2000-02-18 | 2004-08-31 | Elitra Pharmaceuticals Inc. | Gene disruption methodologies for drug target discovery |
| JP5138141B2 (ja) * | 2000-05-19 | 2013-02-06 | ルミネックス コーポレーション | 核酸の検出用物質及び検出方法 |
| US6511809B2 (en) * | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| DE60137649D1 (de) * | 2000-10-14 | 2009-03-26 | Eragen Biosciences Inc | Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen |
| US20030194705A1 (en) | 2002-03-01 | 2003-10-16 | Schroth Gary P. | Methods of using unnatural nucleobases for decoding |
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- 2001-10-15 US US09/977,615 patent/US6977161B2/en not_active Expired - Lifetime
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0742287A2 (en) * | 1995-05-10 | 1996-11-13 | McGall, Glenn H. | Modified nucleic acid probes |
| WO1997046711A1 (en) * | 1996-06-06 | 1997-12-11 | Wisconsin Alumni Research Foundation | Artificial mismatch hybridization |
Also Published As
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|---|---|
| ATE422557T1 (de) | 2009-02-15 |
| US8969047B2 (en) | 2015-03-03 |
| US20070087361A1 (en) | 2007-04-19 |
| CA2425747A1 (en) | 2002-04-25 |
| US20120295808A1 (en) | 2012-11-22 |
| US6977161B2 (en) | 2005-12-20 |
| US20150132759A1 (en) | 2015-05-14 |
| US7892796B2 (en) | 2011-02-22 |
| JP2004531205A (ja) | 2004-10-14 |
| CA2425747C (en) | 2012-01-24 |
| WO2002033126A2 (en) | 2002-04-25 |
| US8518671B2 (en) | 2013-08-27 |
| WO2002033126A3 (en) | 2003-08-28 |
| AU1317502A (en) | 2002-04-29 |
| US20060078936A1 (en) | 2006-04-13 |
| JP4499987B2 (ja) | 2010-07-14 |
| EP1358352B1 (en) | 2009-02-11 |
| US20140024550A1 (en) | 2014-01-23 |
| US8217160B2 (en) | 2012-07-10 |
| EP1358352A2 (en) | 2003-11-05 |
| DE60137649D1 (de) | 2009-03-26 |
| US20040106108A1 (en) | 2004-06-03 |
| US10240189B2 (en) | 2019-03-26 |
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