AU2002213175B2 - Solid support assay systems and methods utilizing non-standard bases - Google Patents

Solid support assay systems and methods utilizing non-standard bases Download PDF

Info

Publication number
AU2002213175B2
AU2002213175B2 AU2002213175A AU2002213175A AU2002213175B2 AU 2002213175 B2 AU2002213175 B2 AU 2002213175B2 AU 2002213175 A AU2002213175 A AU 2002213175A AU 2002213175 A AU2002213175 A AU 2002213175A AU 2002213175 B2 AU2002213175 B2 AU 2002213175B2
Authority
AU
Australia
Prior art keywords
sequence
primer
oligonucleotide
analyte
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU2002213175A
Other languages
English (en)
Other versions
AU2002213175A1 (en
Inventor
Jennifer K. Grenier
David J. Marshall
James R. Prudent
Jerod L. Ptacin
Craig S. Richmond
Eric B. Roesch
Christopher W. Scherrer
Christopher B. Sherrill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Luminex Corp
Original Assignee
Luminex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/861,292 external-priority patent/US7422850B2/en
Application filed by Luminex Corp filed Critical Luminex Corp
Publication of AU2002213175A1 publication Critical patent/AU2002213175A1/en
Application granted granted Critical
Publication of AU2002213175B2 publication Critical patent/AU2002213175B2/en
Assigned to LUMINEX CORPORATION reassignment LUMINEX CORPORATION Request to Amend Deed and Register Assignors: ERAGEN BIOSCIENCES, INC.
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Silicon Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Saccharide Compounds (AREA)
AU2002213175A 2000-10-14 2001-10-15 Solid support assay systems and methods utilizing non-standard bases Expired AU2002213175B2 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US24039700P 2000-10-14 2000-10-14
US60/240,397 2000-10-14
US28283101P 2001-04-10 2001-04-10
US60/282,831 2001-04-10
US09/861,292 2001-05-18
US09/861,292 US7422850B2 (en) 2000-05-19 2001-05-18 Materials and methods for detection of nucleic acids
US29325901P 2001-05-22 2001-05-22
US60/293,259 2001-05-22
PCT/US2001/031993 WO2002033126A2 (en) 2000-10-14 2001-10-15 Solid support assay systems and methods utilizing non-standard bases

Publications (2)

Publication Number Publication Date
AU2002213175A1 AU2002213175A1 (en) 2002-07-04
AU2002213175B2 true AU2002213175B2 (en) 2007-09-13

Family

ID=27500014

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2002213175A Expired AU2002213175B2 (en) 2000-10-14 2001-10-15 Solid support assay systems and methods utilizing non-standard bases
AU1317502A Pending AU1317502A (en) 2000-10-14 2001-10-15 Solid support assay systems and methods utilizing non-standard bases

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU1317502A Pending AU1317502A (en) 2000-10-14 2001-10-15 Solid support assay systems and methods utilizing non-standard bases

Country Status (8)

Country Link
US (6) US6977161B2 (enExample)
EP (1) EP1358352B1 (enExample)
JP (1) JP4499987B2 (enExample)
AT (1) ATE422557T1 (enExample)
AU (2) AU2002213175B2 (enExample)
CA (1) CA2425747C (enExample)
DE (1) DE60137649D1 (enExample)
WO (1) WO2002033126A2 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2409309A1 (en) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
US6977161B2 (en) * 2000-10-14 2005-12-20 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
US20030194705A1 (en) * 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding
DE602004018270D1 (de) * 2003-01-17 2009-01-22 Eragen Biosciences Inc Nukleinsäureamplifikation mit nichtstandardbasen
JP4567673B2 (ja) * 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
US7282333B2 (en) * 2003-10-20 2007-10-16 Promega Corporation Methods and compositions for nucleic acid analysis
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
WO2006101913A2 (en) * 2005-03-18 2006-09-28 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of neiserria
CA2610863C (en) * 2005-06-07 2017-01-17 Eragen Biosciences, Inc. Methods for detection and typing of nucleic acids
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
US7794936B1 (en) * 2006-09-29 2010-09-14 Steven Albert Benner Using thymidine analogs to improve replication in an expanded genetic alphabet
US20080153097A1 (en) * 2006-11-15 2008-06-26 Eragen Biosciences, Inc. Methods and kits for detecting jak2 nucleic acid
US9725713B1 (en) * 2007-01-22 2017-08-08 Steven A Benner In vitro selection with expanded genetic alphabets
WO2008134374A1 (en) * 2007-04-27 2008-11-06 Eragen Biosciences, Inc. Materials and methods for detection of hepatitis c virus
US20100086908A1 (en) * 2007-05-17 2010-04-08 Prudent James R Methods for the detection of respiratory viruses
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
EP2133434A1 (en) 2008-06-12 2009-12-16 Institut National de la Santé et de la Recherche Médicale (INSERM) Method for the detection of target nucleic acid
CN102066574A (zh) * 2008-06-17 2011-05-18 史蒂文·埃尔伯特·奔纳 利用非天然核苷酸扩增dna的过程
US9637783B1 (en) * 2008-06-17 2017-05-02 Steven Benner Amplification of oligonucleotides containing non-standard nucleotides by polymerase chain reactions
US10865431B1 (en) * 2008-06-17 2020-12-15 Steven A Benner Polymerase incorporation of non-standard nucleotides
US20120142004A1 (en) * 2009-05-21 2012-06-07 Siemens Healthcare Diagnostics Inc. Universal Tags With Non-Natural Nucleobases
WO2011043385A1 (ja) 2009-10-06 2011-04-14 独立行政法人理化学研究所 特異な塩基対を形成する人工塩基対
CN102639712A (zh) 2009-10-23 2012-08-15 卢米耐克斯公司 用于提高反应特异性的具有非标准碱基的扩增引物
US10752944B2 (en) 2011-09-06 2020-08-25 Gen-Probe Incorporated Circularized templates for sequencing
EP2753712B1 (en) 2011-09-06 2017-03-22 Gen-Probe Incorporated Closed nucleic acid structures
CN104271771A (zh) * 2012-06-20 2015-01-07 东丽株式会社 核酸的检测方法及核酸检测试剂盒
US20140378327A1 (en) 2013-06-19 2014-12-25 Luminex Corporation Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres
EP3715470B1 (en) * 2013-08-09 2023-10-11 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US9587268B2 (en) * 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9670088B2 (en) 2014-05-20 2017-06-06 Corning Incorporated Scratch resistant glass and method of making
US20150361481A1 (en) 2014-06-13 2015-12-17 Life Technologies Corporation Multiplex nucleic acid amplification
EP4613872A3 (en) 2014-08-11 2025-11-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
JP6688318B2 (ja) 2015-05-01 2020-04-28 ジェン−プローブ・インコーポレーテッド 多重侵入切断アッセイ
WO2017015177A1 (en) 2015-07-17 2017-01-26 Luminex Corporation Methods and compositions for catalytic assays
US10905710B2 (en) 2016-05-24 2021-02-02 Emory University Particles with RNA cleaving nucleobase polymers and uses for managing inflammatory disorders

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0742287A2 (en) * 1995-05-10 1996-11-13 McGall, Glenn H. Modified nucleic acid probes
WO1997046711A1 (en) * 1996-06-06 1997-12-11 Wisconsin Alumni Research Foundation Artificial mismatch hybridization

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
GB8902689D0 (en) 1989-02-07 1989-03-30 Ici Plc Assay method
US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
NZ235032A (en) 1989-08-31 1993-04-28 Dow Chemical Co Constrained geometry complexes of titanium, zirconium or hafnium comprising a substituted cyclopentadiene ligand; use as olefin polymerisation catalyst component
GB8920097D0 (en) 1989-09-06 1989-10-18 Ici Plc Amplification processes
US6007984A (en) 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US6037120A (en) * 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US5965364A (en) * 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US5432272A (en) * 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE4129653A1 (de) * 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
AU3933393A (en) * 1993-03-17 1994-10-11 City Of Hope Nucleic acid sequences having single stranded ends
US6077668A (en) 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5654138A (en) 1993-05-14 1997-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Von hippel-lindau (VHL) disease gene and corresponding cDNA and methods for detecting carriers of the VHL disease gene
US5589329A (en) * 1993-11-15 1996-12-31 Ambion, Inc. Methods and compositions for detecting base pair mismatches
DE69531542T2 (de) * 1994-02-07 2004-06-24 Beckman Coulter, Inc., Fullerton Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5866337A (en) * 1995-03-24 1999-02-02 The Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5736330A (en) * 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5980861A (en) * 1996-03-12 1999-11-09 University Of Massachusetts Chelator compositions and methods of synthesis thereof
AU2320597A (en) * 1996-03-19 1997-10-10 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US6124092A (en) * 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US5853993A (en) 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
JP4185185B2 (ja) 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖dnaを利用したdnaの検出方法
US5928869A (en) 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
CA2308599C (en) 1997-10-28 2012-05-22 The Regents Of The University Of California Dna polymorphism identity determination using flow cytometry
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6046807A (en) * 1998-05-14 2000-04-04 Luminex Corporation Diode laser based measurement apparatus
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
AU2397400A (en) * 1998-12-30 2000-07-31 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
JP2001204497A (ja) * 2000-01-20 2001-07-31 Japan Science & Technology Corp 蛍光共鳴エネルギー移動を利用した、dnaハイブリダイゼーション法によるdna検出方法
US6783985B1 (en) * 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
CA2409309A1 (en) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
US6977161B2 (en) * 2000-10-14 2005-12-20 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US20030194705A1 (en) 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0742287A2 (en) * 1995-05-10 1996-11-13 McGall, Glenn H. Modified nucleic acid probes
WO1997046711A1 (en) * 1996-06-06 1997-12-11 Wisconsin Alumni Research Foundation Artificial mismatch hybridization

Also Published As

Publication number Publication date
US6977161B2 (en) 2005-12-20
US8969047B2 (en) 2015-03-03
CA2425747C (en) 2012-01-24
US20120295808A1 (en) 2012-11-22
EP1358352A2 (en) 2003-11-05
CA2425747A1 (en) 2002-04-25
JP2004531205A (ja) 2004-10-14
US20140024550A1 (en) 2014-01-23
US7892796B2 (en) 2011-02-22
US20060078936A1 (en) 2006-04-13
AU1317502A (en) 2002-04-29
US20040106108A1 (en) 2004-06-03
US8518671B2 (en) 2013-08-27
ATE422557T1 (de) 2009-02-15
US20070087361A1 (en) 2007-04-19
JP4499987B2 (ja) 2010-07-14
WO2002033126A2 (en) 2002-04-25
US20150132759A1 (en) 2015-05-14
DE60137649D1 (de) 2009-03-26
WO2002033126A3 (en) 2003-08-28
US10240189B2 (en) 2019-03-26
EP1358352B1 (en) 2009-02-11
US8217160B2 (en) 2012-07-10

Similar Documents

Publication Publication Date Title
US10240189B2 (en) Solid support assay systems and methods utilizing non-standard bases
AU2002213175A1 (en) Solid support assay systems and methods utilizing non-standard bases
US6127121A (en) Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
US6815164B2 (en) Methods and probes for detection and/or quantification of nucleic acid sequences
US7074569B2 (en) Mobility-modified nucleobase polymers and methods of using same
US7371580B2 (en) Use of unstructured nucleic acids in assaying nucleic acid molecules
JP4510626B2 (ja) 脱塩基部位エンドヌクレアーゼアッセイ
US8053192B2 (en) Methods for indexing samples and sequencing multiple polynucleotide templates
US20100055798A1 (en) Degenerate Nucleobase Analogs
US20020132241A1 (en) Multiplexed detection of analytes
WO2002101358A9 (en) Multiplexed detection methods
CA2481495C (en) Primer extension based method employing nucleotides labelled via cleavable linkers
JP2003507024A (ja) Pna−dnaキメラの3’末端でのポリメラーゼ伸長
US20100112556A1 (en) Method for sample analysis using q probes
EP1590482B1 (en) Nucleic acid amplification using non-standard bases
Nilsson et al. Making ends meet in genetic analysis using padlock probes
EP1520038A2 (en) Detection of circularized immobilized nucleic acid probes
CN100385013C (zh) 使用非标准碱基的固相支持体分析系统和方法
KR102079963B1 (ko) 소거 프로브 기반 염색체 수적이상 검출방법 및 염색체 수적이상 검출용핵산의 조성물
AU2002345657A1 (en) Multiplexed detection methods

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired