AU2001261499A1 - Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins - Google Patents

Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins

Info

Publication number
AU2001261499A1
AU2001261499A1 AU2001261499A AU6149901A AU2001261499A1 AU 2001261499 A1 AU2001261499 A1 AU 2001261499A1 AU 2001261499 A AU2001261499 A AU 2001261499A AU 6149901 A AU6149901 A AU 6149901A AU 2001261499 A1 AU2001261499 A1 AU 2001261499A1
Authority
AU
Australia
Prior art keywords
proteins
libraries
peptides
methods
detecting interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001261499A
Other languages
English (en)
Inventor
Indraneel Ghosh
Andrew D. Hamilton
Lynne Regan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Publication of AU2001261499A1 publication Critical patent/AU2001261499A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2001261499A 2000-05-12 2001-05-14 Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins Abandoned AU2001261499A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20371200P 2000-05-12 2000-05-12
US60203712 2000-05-12
PCT/US2001/015367 WO2001087919A2 (fr) 2000-05-12 2001-05-14 Methodes permettant de detecter des interactions entre des proteines, des peptides ou leurs bibliotheques au moyen de proteines de fusion

Publications (1)

Publication Number Publication Date
AU2001261499A1 true AU2001261499A1 (en) 2001-11-26

Family

ID=22755006

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001261499A Abandoned AU2001261499A1 (en) 2000-05-12 2001-05-14 Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins

Country Status (5)

Country Link
US (2) US6780599B2 (fr)
EP (1) EP1283846A4 (fr)
AU (1) AU2001261499A1 (fr)
CA (1) CA2384561A1 (fr)
WO (1) WO2001087919A2 (fr)

Families Citing this family (38)

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Publication number Priority date Publication date Assignee Title
US7855167B2 (en) * 1998-02-02 2010-12-21 Odyssey Thera, Inc. In vivo screening of protein-protein interactions with protein-fragment complementation assays
US7166424B2 (en) * 1998-02-02 2007-01-23 Odyssey Thera Inc. Fragments of fluorescent proteins for protein fragment complementation assays
WO2001087919A2 (fr) 2000-05-12 2001-11-22 Yale University Methodes permettant de detecter des interactions entre des proteines, des peptides ou leurs bibliotheques au moyen de proteines de fusion
WO2002036738A2 (fr) * 2000-10-30 2002-05-10 Kalobios, Inc. Maturation d'affinite par selection concurrentielle
DE10211653A1 (de) * 2002-03-15 2003-10-02 Klaus Pfizenmaier Filament-Rekrutierung fluoreszierender Proteine zur Analyse und Identifikation von Protein-Protein-Interaktionen: FIT (Filament-based Interaction Trap) analysis
EP1509596A1 (fr) * 2002-04-19 2005-03-02 BioImage A/S Complementation dependante de la translocation pour le criblage de medicaments
JP2006506950A (ja) * 2002-04-19 2006-03-02 バイオイメージ・アクティーゼルスカブ ２つの緑色蛍光タンパク質断片およびタンパク質−タンパク質の相互作用を検出するための方法におけるこれらの使用
US6878523B2 (en) 2002-05-08 2005-04-12 Gentel Bio Surfaces, Inc. Molecular interaction assays on a solid surface
JP4287633B2 (ja) * 2002-09-18 2009-07-01 独立行政法人科学技術振興機構 オルガネラ局在タンパク質の解析方法と解析材料
AU2003301960A1 (en) * 2002-11-05 2004-06-03 Regeneron Pharmaceuticals, Inc. Methods of isolation of active compounds and activated targets
WO2005001115A2 (fr) * 2003-05-30 2005-01-06 Odyssey Thera, Inc. Surveillance de silençage genique et annotation de fonction genique dans des cellules vivantes
DE10343375A1 (de) * 2003-09-17 2005-04-28 Axaron Bioscience Ag Verfahren zur Detektion und Analyse von Protein-Protein-Interaktionen
US7488583B2 (en) * 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
WO2005074436A2 (fr) 2003-10-24 2005-08-18 The Regents Of The University Of California Systemes de proteines fluorescentes fragmentees auto-assembleuses
GB0327143D0 (en) * 2003-11-21 2003-12-24 Univ Belfast Assay
WO2005118790A2 (fr) * 2004-06-03 2005-12-15 The Trustees Of Columbia University In The City Of New York Marquage combinatoire de cellules et de structures cellulaires de proteines fluorescentes reconstituees
US20060094059A1 (en) * 2004-09-22 2006-05-04 Odyssey Thera, Inc. Methods for identifying new drug leads and new therapeutic uses for known drugs
US20070212677A1 (en) * 2004-11-22 2007-09-13 Odyssey Thera, Inc. Identifying off-target effects and hidden phenotypes of drugs in human cells
AU2005314236A1 (en) * 2004-12-04 2006-06-15 The Regents Of The University Of California Los Alamos National Laboratory Protein-protein interaction detection system using fluorescent protein microdomains
CA2638888A1 (fr) * 2004-12-04 2006-06-15 The Regents Of The University Of California Bioessais de localisation d'elements subcellulaires de proteines utilisant des proteines fluorescentes scindees
EP1931787A4 (fr) * 2005-09-30 2010-10-20 Univ Montana State Systeme de detection d'interactions proteines-proteines
WO2008018905A2 (fr) * 2006-01-17 2008-02-14 Cellumen, Inc. Procédé permettant de prédire les réponses des systèmes biologiques
US8114615B2 (en) 2006-05-17 2012-02-14 Cernostics, Inc. Method for automated tissue analysis
JPWO2007142208A1 (ja) * 2006-06-05 2009-10-22 国立大学法人京都大学 温度感応タンパク質、ポリヌクレオチド、温度感応タンパク質発現プラスミド及び発現細胞、温度感応タンパク質の利用方法
US20080299599A1 (en) * 2006-09-07 2008-12-04 Robert Dirksen Fluorescent proteins for monitoring intracellular superoxide production
WO2008060483A2 (fr) * 2006-11-10 2008-05-22 Cellumen, Inc. Biocapteurs d'interaction protéine-protéine et leurs procédés d'utilisation
CA2704226A1 (fr) * 2007-11-01 2009-05-07 The Arizona Board Of Regents On Behalf Of The University Of Arizona Procedes acellulaires destines a detecter des interactions proteine-ligand
US20100137563A1 (en) 2008-12-03 2010-06-03 Northwestern University Cysteine Protease Autoprocessing of Fusion Proteins
US8524457B2 (en) 2009-09-22 2013-09-03 William Patterson Method for the selection of specific affinity binders by homogeneous noncompetitive assay
WO2011046521A1 (fr) * 2009-10-15 2011-04-21 Kemijski inštitut Structures auto-assemblées composées d'un polypeptide unique comprenant au moins trois éléments formant une super-hélice
KR101272817B1 (ko) * 2010-04-29 2013-06-10 한국생명공학연구원 항체 생산 세포주 선별 방법 및 선별 키트
WO2011136602A2 (fr) * 2010-04-29 2011-11-03 한국생명공학연구원 Procédé pour sélectionner une lignée de cellules produisant des anticorps, et trousse correspondante
US10018631B2 (en) 2011-03-17 2018-07-10 Cernostics, Inc. Systems and compositions for diagnosing Barrett's esophagus and methods of using the same
SG10201600144YA (en) * 2011-12-05 2016-02-26 Med & Biological Lab Co Ltd Method for detecting protein-protein interaction
WO2018089602A1 (fr) * 2016-11-09 2018-05-17 Baylor College Of Medicine Agent thérapeutique destiné à la prévention et/ou au traitement de la prise de poids et/ou du diabète
CN108732359B (zh) * 2017-04-20 2020-09-15 厦门大学 一种检测系统
KR102541652B1 (ko) * 2020-03-31 2023-06-13 바이오디자인랩 주식회사 히스톤 또는 루신 지퍼의 인테그라제로의 삽입을 통한 안전한 레트로바이러스 벡터 구축 방법
WO2023173028A2 (fr) * 2022-03-09 2023-09-14 The Regents Of The University Of Colorado A Body Corporate Administration spécifique de tissu et de cellule de molécules thérapeutiques incorporant des protéines fusiogènes virales et humaines

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2196496A1 (fr) 1997-01-31 1998-07-31 Stephen William Watson Michnick Epreuve de complementation de fragments de proteines pour la detection d'interactions entre proteines
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6180343B1 (en) 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
ATE459724T1 (de) 1999-06-26 2010-03-15 Odyssey Thera Inc EINE IN VIVO ßBIBLIOTHEK-GEGEN-BIBLIOTHEK- SELEKTIONß VON OPTIMIERTEN PROTEIN-PROTEIN- WECHSELWIRKUNGEN
AU6058500A (en) * 1999-06-30 2001-01-31 Center For Blood Research, The Fusion protein and uses thereof
WO2001087919A2 (fr) 2000-05-12 2001-11-22 Yale University Methodes permettant de detecter des interactions entre des proteines, des peptides ou leurs bibliotheques au moyen de proteines de fusion

Also Published As

Publication number Publication date
EP1283846A4 (fr) 2005-06-01
WO2001087919A3 (fr) 2002-05-30
EP1283846A2 (fr) 2003-02-19
WO2001087919A2 (fr) 2001-11-22
US20040235064A1 (en) 2004-11-25
US6780599B2 (en) 2004-08-24
US20020146701A1 (en) 2002-10-10
CA2384561A1 (fr) 2001-11-22
US7176287B2 (en) 2007-02-13

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