ATE74137T1 - Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie. - Google Patents

Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie.

Info

Publication number
ATE74137T1
ATE74137T1 AT87114755T AT87114755T ATE74137T1 AT E74137 T1 ATE74137 T1 AT E74137T1 AT 87114755 T AT87114755 T AT 87114755T AT 87114755 T AT87114755 T AT 87114755T AT E74137 T1 ATE74137 T1 AT E74137T1
Authority
AT
Austria
Prior art keywords
toxin
unconjugated
purification
procedure
toxin conjugates
Prior art date
Application number
AT87114755T
Other languages
English (en)
Inventor
Robert Ferris
Walter Joseph Laird
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Application granted granted Critical
Publication of ATE74137T1 publication Critical patent/ATE74137T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
    • A61K47/6817—Toxins
    • A61K47/6819—Plant toxins
    • A61K47/6825—Ribosomal inhibitory proteins, i.e. RIP-I or RIP-II, e.g. Pap, gelonin or dianthin
    • A61K47/6827—Ricin A

Landscapes

  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT87114755T 1986-10-10 1987-10-09 Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie. ATE74137T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/917,469 US4771128A (en) 1986-10-10 1986-10-10 Method of purifying toxin conjugates using hydrophobic interaction chromatography
EP87114755A EP0263526B1 (de) 1986-10-10 1987-10-09 Verfahren zur Reinigung von Toxinconjugaten unter Verwendung von hydrophober Chromatographie

Publications (1)

Publication Number Publication Date
ATE74137T1 true ATE74137T1 (de) 1992-04-15

Family

ID=25438836

Family Applications (1)

Application Number Title Priority Date Filing Date
AT87114755T ATE74137T1 (de) 1986-10-10 1987-10-09 Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie.

Country Status (12)

Country Link
US (1) US4771128A (de)
EP (1) EP0263526B1 (de)
JP (1) JPS63115900A (de)
AT (1) ATE74137T1 (de)
AU (1) AU611397B2 (de)
CA (1) CA1305415C (de)
DE (1) DE3777744D1 (de)
DK (1) DK530587A (de)
ES (1) ES2031101T3 (de)
FI (1) FI874463L (de)
GR (1) GR3004145T3 (de)
NO (1) NO874137L (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962189A (en) * 1985-06-20 1990-10-09 Cetus Corportion Ribotoxin conjugates
GB8725606D0 (en) * 1987-11-02 1987-12-09 Soc D Etudes Prod Chimique Preparation polynucleotides
AU3750989A (en) * 1988-06-01 1990-01-05 Cetus Corporation One-step chromatographic purification of immunotoxin conjugates using a thiophilic matrix
IE62463B1 (en) * 1988-07-07 1995-02-08 Res Dev Foundation Immunoconjugates for cancer diagnosis and therapy
IL91933A (en) * 1988-10-11 1994-12-29 Univ Southern California Vasoactive immunoconjugates useful for increasing the vascular permeability or the blood supply to neoplastic or otherwise diseased tissues
US6750329B1 (en) * 1989-05-05 2004-06-15 Research Development Foundation Antibody delivery system for biological response modifiers
US5101018A (en) * 1989-06-12 1992-03-31 International Minerals & Chemical Corp. Method for recovering recombinant proteins
US5208323A (en) * 1989-08-10 1993-05-04 Universite Laval Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent
US5047511A (en) * 1989-08-28 1991-09-10 Pitman-Moore, Inc. Method for recovering recombinant proteins
US5110912A (en) * 1990-03-02 1992-05-05 Seragen, Inc. Purification of il-2-containing hybrid compounds
ZA912490B (en) * 1990-04-19 1992-12-30 Res Dev Foundation Antibody conjugates for treatment of neoplastic disease
US5110913A (en) * 1990-05-25 1992-05-05 Miles Inc. Antibody purification method
US5194594A (en) * 1990-09-07 1993-03-16 Techniclone, Inc. Modified antibodies
GB9108085D0 (en) * 1991-04-16 1991-06-05 Scras Complexes of polyadenylic acid with polyuridylic acid
US5252216A (en) * 1992-03-24 1993-10-12 Smithkline Beecham Corporation Protein purification
US5811403A (en) * 1996-09-30 1998-09-22 Vanderbilt University Polysaccharide toxin from Group B β-hemolytic Streptococcus (GBS) having improved purity
ZA200305980B (en) * 2001-02-12 2007-01-31 Res Dev Foundation Modified proteins, designer toxins, and methods of making thereof
NZ530582A (en) * 2001-07-17 2008-06-30 Res Dev Foundation Therapeutic agents comprising pro-apoptotic proteins, including granzyme, bax and TNF
SG187991A1 (en) 2002-05-02 2013-03-28 Wyeth Corp Calicheamicin derivative-carrier conjugates
JP2006506964A (ja) * 2002-06-12 2006-03-02 リサーチ　ディベロップメント　ファンデーション 治療剤としての免疫毒素及びその利用
US7329353B2 (en) * 2004-01-23 2008-02-12 Amgen Inc. LC/MS method of analyzing high molecular weight proteins
CN101132813A (zh) * 2005-02-01 2008-02-27 研究发展基金会 靶向blys受体的blys融合蛋白和用于治疗b细胞增殖性疾病的方法
US9901567B2 (en) 2007-08-01 2018-02-27 Syntarga B.V. Substituted CC-1065 analogs and their conjugates
WO2010101865A1 (en) 2009-03-02 2010-09-10 Zymo Research Corporation Universal column
JP5699429B2 (ja) * 2009-12-18 2015-04-08 東ソー株式会社 Ｆｃレセプターの精製方法
CA2956934C (en) 2010-04-21 2019-07-09 Syntarga B.V. Novel conjugates of cc-1065 analogs and bifunctional linkers
KR20140129221A (ko) * 2012-05-21 2014-11-06 에스알씨, 인코퍼레이트 리신과 기타 리보솜 비활성화 단백질의 검출을 위한 방법과 시스템
AU2015205574B2 (en) * 2014-01-10 2019-08-15 Byondis B.V. Method for purifying Cys-linked antibody-drug conjugates
HRP20160797T1 (hr) 2014-01-10 2016-08-12 Synthon Biopharmaceuticals B.V. Duokarmicin adsc, namijenjen upotrebi u liječenju raka endometrija
MX368396B (es) 2014-01-10 2019-10-01 Synthon Biopharmaceuticals Bv Conjugados de anticuerpo-farmaco (adc) de duocarmicina que muestran actividad antitumoral in vivo mejorada.

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1907014A1 (de) * 1969-02-12 1970-09-03 Haller Dr Wolfgang Verfahren zur Trennung von Blutkomponenten,insbesondere von immunologisch aktiven Globulinen,von anderen Komponenten
US4000098A (en) * 1974-08-16 1976-12-28 Palo Alto Medical Research Foundation Separation of proteins by hydrophobic adsorption
JPS5436201A (en) * 1977-08-25 1979-03-16 Microbial Chem Res Found New oncostatic antibiotic auromomycin and its preparation
FR2437213A1 (fr) * 1978-09-28 1980-04-25 Cm Ind Produits cytotoxiques formes par liaison covalente de la chaine a de la ricine avec un anticorps et leur procede de preparation
US4315851A (en) * 1978-12-29 1982-02-16 Kureha Kagaku Kogyo Kabushiki Kaisha Pharmaceutical composition having antitumor activity
US4276206A (en) * 1979-01-18 1981-06-30 Scripps Clinic & Research Foundation Immunochemical conjugates: method and composition
JPS5616418A (en) * 1979-07-20 1981-02-17 Teijin Ltd Antitumor protein complex and its preparation
US4355023A (en) * 1980-09-30 1982-10-19 The Massachusetts General Hospital Antibody fragment compositions and process
FR2504010B1 (fr) * 1981-04-15 1985-10-25 Sanofi Sa Medicaments anticancereux contenant la chaine a de la ricine associee a un anticorps antimelanome et procede pour leur preparation
FR2516794B1 (fr) * 1981-11-20 1985-10-25 Sanofi Sa Nouveaux medicaments anticancereux pour le traitement des leucemies t constitues de la chaine a de la ricine et d'un anticorps monoclonal specifique
US4461833A (en) * 1982-06-23 1984-07-24 University Patents, Inc. Chromatographically purifying proteolytic procoagulant enzyme from animal tissue extract
US4579941A (en) * 1982-08-13 1986-04-01 Mitsuitoatsu Chemicals, Inc. Thermally-denatured deoxyribonucleic acid MD-011 and antitumor agent
US4568488A (en) * 1984-01-11 1986-02-04 Lee Huang Sylvia Reverse immunoaffinity chromatography purification method
DE3426049A1 (de) * 1984-07-14 1986-03-06 Boehringer Ingelheim International GmbH, 6507 Ingelheim Humaner-tumor-nekrose-faktor
US4677197A (en) * 1985-10-30 1987-06-30 Cetus Corporation Purification method for tumor necrosis factor
CA1286843C (en) * 1985-10-30 1991-07-23 Leo S. Lin Purification method for proteins
US4689401A (en) * 1986-03-06 1987-08-25 Cetus Corporation Method of recovering microbially produced recombinant ricin toxin a chain
DE3650692T2 (de) * 1986-03-07 1999-04-22 Chiron Corp., Emeryville, Calif. Rekombinantes Ricin-Toxin

Also Published As

Publication number Publication date
DK530587A (da) 1988-04-11
DE3777744D1 (de) 1992-04-30
ES2031101T3 (es) 1992-12-01
FI874463A7 (fi) 1988-04-11
EP0263526A1 (de) 1988-04-13
DK530587D0 (da) 1987-10-09
GR3004145T3 (de) 1993-03-31
AU611397B2 (en) 1991-06-13
FI874463L (fi) 1988-04-11
NO874137L (no) 1988-04-11
FI874463A0 (fi) 1987-10-09
CA1305415C (en) 1992-07-21
NO874137D0 (no) 1987-10-01
JPS63115900A (ja) 1988-05-20
EP0263526B1 (de) 1992-03-25
US4771128A (en) 1988-09-13
AU7952787A (en) 1988-04-14

Similar Documents

Publication Publication Date Title
DE3777744D1 (de) Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie.
DE59106519D1 (de) Verfahren zur chromatographischen Reinigung von Insulinen.
ATE200293T1 (de) Verfahren zur chromatographischen reinigung und trennung von nucleinsäuregemischen
DK575184D0 (da) Fremgangsmaade til oprensning af factor viii.c samt farmaceutisk praeparat indeholdende denne
ATE46269T1 (de) Verfahren zur bodendekontaminierung mittels mikroorganismen.
ATE124703T1 (de) Verfahren zur trennung von proteinen.
SG52497A1 (en) Separation procedure for LP(A) by means of electrophoresis gels for the implementation of this procedure & application to the in vitro (pls see file for full title)
DE3887095D1 (de) Verfahren zur Isolierung und Reinigung von Hirudin.
DE3865777D1 (de) Verfahren zur reinigung von olivenoel.
DE69219384D1 (de) Packungen mit über Spacer an Träger gebundenem Protein
ATE20071T1 (de) Verfahren zur reinigung von sekretin.
EP0603671A3 (de) Verfahren zur Reaktivierung von gereinigten Membranproteinen.
DE69300471D1 (de) Verfahren zur Herstellung von Derivaten der epoxypropionsäure.
FI891121A7 (fi) Menetelmä tekijä XIII:n puhdistamiseksi affiniteettikromatografialla
AU7955687A (en) Method for purification of antibodies
DE3785852D1 (de) Verfahren zur chromatographischen trennung von metallionen.
DE3780395D1 (de) Zusammensetzung zur absorption von quecksilber.
ATE231158T1 (de) Verfahren zur reinigung von daunomycin
PL140464B2 (en) Method of modifying porous polymer sorbents for chromatography
DE3675636D1 (de) Verfahren zum herstellen von elementarphosphor unter geringer entwicklung von fluechtigem fluor.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEFA Change of the company name
EEIH Change in the person of patent owner
REN Ceased due to non-payment of the annual fee