ATE74137T1 - Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie. - Google Patents
Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie.Info
- Publication number
- ATE74137T1 ATE74137T1 AT87114755T AT87114755T ATE74137T1 AT E74137 T1 ATE74137 T1 AT E74137T1 AT 87114755 T AT87114755 T AT 87114755T AT 87114755 T AT87114755 T AT 87114755T AT E74137 T1 ATE74137 T1 AT E74137T1
- Authority
- AT
- Austria
- Prior art keywords
- toxin
- unconjugated
- purification
- procedure
- toxin conjugates
- Prior art date
Links
- 239000002596 immunotoxin Substances 0.000 title abstract 3
- 230000002209 hydrophobic effect Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000004587 chromatography analysis Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 239000012266 salt solution Substances 0.000 abstract 2
- 239000003053 toxin Substances 0.000 abstract 2
- 231100000765 toxin Toxicity 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
- A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
- A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
- A61K47/6817—Toxins
- A61K47/6819—Plant toxins
- A61K47/6825—Ribosomal inhibitory proteins, i.e. RIP-I or RIP-II, e.g. Pap, gelonin or dianthin
- A61K47/6827—Ricin A
Landscapes
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Toxicology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/917,469 US4771128A (en) | 1986-10-10 | 1986-10-10 | Method of purifying toxin conjugates using hydrophobic interaction chromatography |
| EP87114755A EP0263526B1 (de) | 1986-10-10 | 1987-10-09 | Verfahren zur Reinigung von Toxinconjugaten unter Verwendung von hydrophober Chromatographie |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE74137T1 true ATE74137T1 (de) | 1992-04-15 |
Family
ID=25438836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT87114755T ATE74137T1 (de) | 1986-10-10 | 1987-10-09 | Verfahren zur reinigung von toxinconjugaten unter verwendung von hydrophober chromatographie. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US4771128A (de) |
| EP (1) | EP0263526B1 (de) |
| JP (1) | JPS63115900A (de) |
| AT (1) | ATE74137T1 (de) |
| AU (1) | AU611397B2 (de) |
| CA (1) | CA1305415C (de) |
| DE (1) | DE3777744D1 (de) |
| DK (1) | DK530587A (de) |
| ES (1) | ES2031101T3 (de) |
| FI (1) | FI874463A (de) |
| GR (1) | GR3004145T3 (de) |
| NO (1) | NO874137L (de) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4962189A (en) * | 1985-06-20 | 1990-10-09 | Cetus Corportion | Ribotoxin conjugates |
| GB8725606D0 (en) * | 1987-11-02 | 1987-12-09 | Soc D Etudes Prod Chimique | Preparation polynucleotides |
| WO1989011871A1 (en) * | 1988-06-01 | 1989-12-14 | Cetus Corporation | One-step chromatographic purification of immunotoxin conjugates using a thiophilic matrix |
| IE62463B1 (en) * | 1988-07-07 | 1995-02-08 | Res Dev Foundation | Immunoconjugates for cancer diagnosis and therapy |
| IL91933A (en) * | 1988-10-11 | 1994-12-29 | Univ Southern California | Their immuno-bracelet and isophiles which are beneficial in increasing vascular permeability or blood supply to tumor or otherwise damaged tissue |
| US6750329B1 (en) * | 1989-05-05 | 2004-06-15 | Research Development Foundation | Antibody delivery system for biological response modifiers |
| US5101018A (en) * | 1989-06-12 | 1992-03-31 | International Minerals & Chemical Corp. | Method for recovering recombinant proteins |
| US5208323A (en) * | 1989-08-10 | 1993-05-04 | Universite Laval | Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent |
| US5047511A (en) * | 1989-08-28 | 1991-09-10 | Pitman-Moore, Inc. | Method for recovering recombinant proteins |
| US5110912A (en) * | 1990-03-02 | 1992-05-05 | Seragen, Inc. | Purification of il-2-containing hybrid compounds |
| IE62496B1 (en) * | 1990-04-19 | 1995-02-08 | Res Dev Foundation | Antibody conjugates for treatment of neoplastic disease |
| US5110913A (en) * | 1990-05-25 | 1992-05-05 | Miles Inc. | Antibody purification method |
| US5194594A (en) * | 1990-09-07 | 1993-03-16 | Techniclone, Inc. | Modified antibodies |
| GB9108085D0 (en) * | 1991-04-16 | 1991-06-05 | Scras | Complexes of polyadenylic acid with polyuridylic acid |
| US5252216A (en) * | 1992-03-24 | 1993-10-12 | Smithkline Beecham Corporation | Protein purification |
| US5811403A (en) * | 1996-09-30 | 1998-09-22 | Vanderbilt University | Polysaccharide toxin from Group B β-hemolytic Streptococcus (GBS) having improved purity |
| ZA200305980B (en) * | 2001-02-12 | 2007-01-31 | Res Dev Foundation | Modified proteins, designer toxins, and methods of making thereof |
| US7101977B2 (en) * | 2001-07-17 | 2006-09-05 | Research Development Foundation | Therapeutic agents comprising pro-apoptotic proteins |
| PL224001B1 (pl) | 2002-05-02 | 2016-11-30 | Wyeth Corp | Sposób wytwarzania stabilnej liofilizowanej kompozycji obejmującej monomeryczne koniugaty pochodna kalicheamycyny/przeciwciało anty-CD22, kompozycja otrzymana tym sposobem oraz jej zastosowanie |
| CA2488858A1 (en) * | 2002-06-12 | 2003-12-24 | Research Development Foundation | Immunotoxin as a therapeutic agent and uses thereof |
| US7329353B2 (en) * | 2004-01-23 | 2008-02-12 | Amgen Inc. | LC/MS method of analyzing high molecular weight proteins |
| WO2006083961A2 (en) * | 2005-02-01 | 2006-08-10 | Research Development Foundation | Blys fusion proteins for targeting blys receptor and methods for treatment of b-cell proliferative disorders |
| US9901567B2 (en) | 2007-08-01 | 2018-02-27 | Syntarga B.V. | Substituted CC-1065 analogs and their conjugates |
| US20100222560A1 (en) | 2009-03-02 | 2010-09-02 | Zymo Research Corporation | Universal column |
| JP5699429B2 (ja) * | 2009-12-18 | 2015-04-08 | 東ソー株式会社 | Ｆｃレセプターの精製方法 |
| LT2560645T (lt) | 2010-04-21 | 2016-10-10 | Syntarga B.V. | Cc-1065 analogų ir bifunkcinių linkerių konjugatai |
| US10907193B2 (en) * | 2012-05-21 | 2021-02-02 | Acumen Detection, Llc | Methods and systems for the detection of ricin and other ribosome inactivating proteins |
| DK2948184T3 (en) | 2014-01-10 | 2016-06-27 | Synthon Biopharmaceuticals Bv | Duocarmycin-ADCs with improved in vivo antitumor activity |
| KR102323301B1 (ko) * | 2014-01-10 | 2021-11-09 | 비온디스 비.브이. | Cys 연결된 항체-약물 접합체의 정제 방법 |
| CA2935430C (en) | 2014-01-10 | 2018-09-18 | Synthon Biopharmaceuticals B.V. | Duocarmycin adcs for use in treatment of endometrial cancer |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1907014A1 (de) * | 1969-02-12 | 1970-09-03 | Haller Dr Wolfgang | Verfahren zur Trennung von Blutkomponenten,insbesondere von immunologisch aktiven Globulinen,von anderen Komponenten |
| US4000098A (en) * | 1974-08-16 | 1976-12-28 | Palo Alto Medical Research Foundation | Separation of proteins by hydrophobic adsorption |
| JPS5436201A (en) * | 1977-08-25 | 1979-03-16 | Microbial Chem Res Found | New oncostatic antibiotic auromomycin and its preparation |
| FR2437213A1 (fr) * | 1978-09-28 | 1980-04-25 | Cm Ind | Produits cytotoxiques formes par liaison covalente de la chaine a de la ricine avec un anticorps et leur procede de preparation |
| US4315851A (en) * | 1978-12-29 | 1982-02-16 | Kureha Kagaku Kogyo Kabushiki Kaisha | Pharmaceutical composition having antitumor activity |
| US4276206A (en) * | 1979-01-18 | 1981-06-30 | Scripps Clinic & Research Foundation | Immunochemical conjugates: method and composition |
| JPS5616418A (en) * | 1979-07-20 | 1981-02-17 | Teijin Ltd | Antitumor protein complex and its preparation |
| US4355023A (en) * | 1980-09-30 | 1982-10-19 | The Massachusetts General Hospital | Antibody fragment compositions and process |
| FR2504010B1 (fr) * | 1981-04-15 | 1985-10-25 | Sanofi Sa | Medicaments anticancereux contenant la chaine a de la ricine associee a un anticorps antimelanome et procede pour leur preparation |
| FR2516794B1 (fr) * | 1981-11-20 | 1985-10-25 | Sanofi Sa | Nouveaux medicaments anticancereux pour le traitement des leucemies t constitues de la chaine a de la ricine et d'un anticorps monoclonal specifique |
| US4461833A (en) * | 1982-06-23 | 1984-07-24 | University Patents, Inc. | Chromatographically purifying proteolytic procoagulant enzyme from animal tissue extract |
| US4579941A (en) * | 1982-08-13 | 1986-04-01 | Mitsuitoatsu Chemicals, Inc. | Thermally-denatured deoxyribonucleic acid MD-011 and antitumor agent |
| US4568488A (en) * | 1984-01-11 | 1986-02-04 | Lee Huang Sylvia | Reverse immunoaffinity chromatography purification method |
| DE3426049A1 (de) * | 1984-07-14 | 1986-03-06 | Boehringer Ingelheim International GmbH, 6507 Ingelheim | Humaner-tumor-nekrose-faktor |
| CA1286843C (en) * | 1985-10-30 | 1991-07-23 | Leo S. Lin | Purification method for proteins |
| US4677197A (en) * | 1985-10-30 | 1987-06-30 | Cetus Corporation | Purification method for tumor necrosis factor |
| US4689401A (en) * | 1986-03-06 | 1987-08-25 | Cetus Corporation | Method of recovering microbially produced recombinant ricin toxin a chain |
| AU601956B2 (en) * | 1986-03-07 | 1990-09-27 | Cetus Corporation | Recombinant ricin toxin |
-
1986
- 1986-10-10 US US06/917,469 patent/US4771128A/en not_active Expired - Fee Related
-
1987
- 1987-09-11 CA CA000546651A patent/CA1305415C/en not_active Expired - Fee Related
- 1987-10-01 NO NO874137A patent/NO874137L/no unknown
- 1987-10-09 DK DK530587A patent/DK530587A/da not_active Application Discontinuation
- 1987-10-09 FI FI874463A patent/FI874463A/fi not_active Application Discontinuation
- 1987-10-09 EP EP87114755A patent/EP0263526B1/de not_active Expired - Lifetime
- 1987-10-09 ES ES198787114755T patent/ES2031101T3/es not_active Expired - Lifetime
- 1987-10-09 AT AT87114755T patent/ATE74137T1/de not_active IP Right Cessation
- 1987-10-09 AU AU79527/87A patent/AU611397B2/en not_active Expired - Fee Related
- 1987-10-09 DE DE8787114755T patent/DE3777744D1/de not_active Expired - Fee Related
- 1987-10-12 JP JP62254796A patent/JPS63115900A/ja active Pending
-
1992
- 1992-03-26 GR GR910402197T patent/GR3004145T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FI874463A (fi) | 1988-04-11 |
| DE3777744D1 (de) | 1992-04-30 |
| AU611397B2 (en) | 1991-06-13 |
| DK530587A (da) | 1988-04-11 |
| ES2031101T3 (es) | 1992-12-01 |
| US4771128A (en) | 1988-09-13 |
| FI874463A0 (fi) | 1987-10-09 |
| CA1305415C (en) | 1992-07-21 |
| DK530587D0 (da) | 1987-10-09 |
| EP0263526B1 (de) | 1992-03-25 |
| AU7952787A (en) | 1988-04-14 |
| JPS63115900A (ja) | 1988-05-20 |
| EP0263526A1 (de) | 1988-04-13 |
| NO874137D0 (no) | 1987-10-01 |
| NO874137L (no) | 1988-04-11 |
| GR3004145T3 (de) | 1993-03-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EEFA | Change of the company name | ||
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |