ES2031101T3 - Metodo de purificar conjugados de toxinas usando cromatografia de interaccion hidrofoba. - Google Patents
Metodo de purificar conjugados de toxinas usando cromatografia de interaccion hidrofoba.Info
- Publication number
- ES2031101T3 ES2031101T3 ES198787114755T ES87114755T ES2031101T3 ES 2031101 T3 ES2031101 T3 ES 2031101T3 ES 198787114755 T ES198787114755 T ES 198787114755T ES 87114755 T ES87114755 T ES 87114755T ES 2031101 T3 ES2031101 T3 ES 2031101T3
- Authority
- ES
- Spain
- Prior art keywords
- conjugates
- interaction chromatography
- hydrophoba
- toxin
- toxin conjugates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002596 immunotoxin Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000004587 chromatography analysis Methods 0.000 title 1
- 230000003993 interaction Effects 0.000 title 1
- 239000003053 toxin Substances 0.000 abstract 2
- 231100000765 toxin Toxicity 0.000 abstract 2
- 108700012359 toxins Proteins 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
- A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
- A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
- A61K47/6817—Toxins
- A61K47/6819—Plant toxins
- A61K47/6825—Ribosomal inhibitory proteins, i.e. RIP-I or RIP-II, e.g. Pap, gelonin or dianthin
- A61K47/6827—Ricin A
Landscapes
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Toxicology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
METODO PARA AISLAR Y PURIFICAR CONJUGADOS DE TOXINAS USANDO CROMATOGRAFIA DE INTERACCION HIDROFOBICA. LAS MEZCLAS CRUDAS DE CONJUGADOS SE AJUSTAN PARA SEPARAR LA TOXINA NO CONJUGADA Y SE CARGAN EN UNA COLUMNA RELLENA CON UN GEL HIDROFOBICO ADECUADO LA ELUCION SE EFECTUA CON SOLUCIONES SALINAS DE FUERZA IONICA DECRECIENTE LAS CUALES OPCIONALMENTE INCLUYEN CANTIDADES CRECIENTES DE UN DISOLVENTE ORGANICO. SE OBTIENEN CONJUGADOS DE TOXINAS SUSTANCIALMENTE EXENTOS DE INMUNOGLOBULINA Y DE TOXINA NO CONJUGADAS
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/917,469 US4771128A (en) | 1986-10-10 | 1986-10-10 | Method of purifying toxin conjugates using hydrophobic interaction chromatography |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2031101T3 true ES2031101T3 (es) | 1992-12-01 |
Family
ID=25438836
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES198787114755T Expired - Lifetime ES2031101T3 (es) | 1986-10-10 | 1987-10-09 | Metodo de purificar conjugados de toxinas usando cromatografia de interaccion hidrofoba. |
Country Status (12)
Country | Link |
---|---|
US (1) | US4771128A (es) |
EP (1) | EP0263526B1 (es) |
JP (1) | JPS63115900A (es) |
AT (1) | ATE74137T1 (es) |
AU (1) | AU611397B2 (es) |
CA (1) | CA1305415C (es) |
DE (1) | DE3777744D1 (es) |
DK (1) | DK530587A (es) |
ES (1) | ES2031101T3 (es) |
FI (1) | FI874463A (es) |
GR (1) | GR3004145T3 (es) |
NO (1) | NO874137L (es) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4962189A (en) * | 1985-06-20 | 1990-10-09 | Cetus Corportion | Ribotoxin conjugates |
GB8725606D0 (en) * | 1987-11-02 | 1987-12-09 | Soc D Etudes Prod Chimique | Preparation polynucleotides |
AU3750989A (en) * | 1988-06-01 | 1990-01-05 | Cetus Corporation | One-step chromatographic purification of immunotoxin conjugates using a thiophilic matrix |
IE62463B1 (en) * | 1988-07-07 | 1995-02-08 | Res Dev Foundation | Immunoconjugates for cancer diagnosis and therapy |
IL91933A (en) * | 1988-10-11 | 1994-12-29 | Univ Southern California | Their immuno-bracelet and isophiles which are beneficial in increasing vascular permeability or blood supply to tumor or otherwise damaged tissue |
US6750329B1 (en) * | 1989-05-05 | 2004-06-15 | Research Development Foundation | Antibody delivery system for biological response modifiers |
US5101018A (en) * | 1989-06-12 | 1992-03-31 | International Minerals & Chemical Corp. | Method for recovering recombinant proteins |
US5208323A (en) * | 1989-08-10 | 1993-05-04 | Universite Laval | Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent |
US5047511A (en) * | 1989-08-28 | 1991-09-10 | Pitman-Moore, Inc. | Method for recovering recombinant proteins |
US5110912A (en) * | 1990-03-02 | 1992-05-05 | Seragen, Inc. | Purification of il-2-containing hybrid compounds |
NZ237688A (en) * | 1990-04-19 | 1993-01-27 | Res Dev Foundation | Antibody-cytotoxic immunoconjugate-containing compositions and cancer treatment |
US5110913A (en) * | 1990-05-25 | 1992-05-05 | Miles Inc. | Antibody purification method |
US5194594A (en) * | 1990-09-07 | 1993-03-16 | Techniclone, Inc. | Modified antibodies |
GB9108085D0 (en) * | 1991-04-16 | 1991-06-05 | Scras | Complexes of polyadenylic acid with polyuridylic acid |
US5252216A (en) * | 1992-03-24 | 1993-10-12 | Smithkline Beecham Corporation | Protein purification |
US5811403A (en) * | 1996-09-30 | 1998-09-22 | Vanderbilt University | Polysaccharide toxin from Group B β-hemolytic Streptococcus (GBS) having improved purity |
ZA200305980B (en) * | 2001-02-12 | 2007-01-31 | Res Dev Foundation | Modified proteins, designer toxins, and methods of making thereof |
JP2004535202A (ja) * | 2001-07-17 | 2004-11-25 | リサーチ ディベロップメント ファンデーション | アポトーシス促進性蛋白質を含む治療剤 |
PL224001B1 (pl) * | 2002-05-02 | 2016-11-30 | Wyeth Corp | Sposób wytwarzania stabilnej liofilizowanej kompozycji obejmującej monomeryczne koniugaty pochodna kalicheamycyny/przeciwciało anty-CD22, kompozycja otrzymana tym sposobem oraz jej zastosowanie |
WO2003105761A2 (en) * | 2002-06-12 | 2003-12-24 | Research Development Foundation | Immunotoxin as a therapeutic agent and uses thereof |
US7329353B2 (en) * | 2004-01-23 | 2008-02-12 | Amgen Inc. | LC/MS method of analyzing high molecular weight proteins |
CA2595904A1 (en) * | 2005-02-01 | 2006-08-10 | Research Development Foundation | Blys fusion proteins for targeting blys receptor and methods for treatment of b-cell proliferative disorders |
US9901567B2 (en) | 2007-08-01 | 2018-02-27 | Syntarga B.V. | Substituted CC-1065 analogs and their conjugates |
WO2010101865A1 (en) | 2009-03-02 | 2010-09-10 | Zymo Research Corporation | Universal column |
JP5699429B2 (ja) * | 2009-12-18 | 2015-04-08 | 東ソー株式会社 | Fcレセプターの精製方法 |
PL3056203T3 (pl) | 2010-04-21 | 2018-06-29 | Syntarga B.V. | Koniugaty analogów CC-1065 i łączników dwufunkcyjnych |
US10907193B2 (en) * | 2012-05-21 | 2021-02-02 | Acumen Detection, Llc | Methods and systems for the detection of ricin and other ribosome inactivating proteins |
WO2015104385A2 (en) | 2014-01-10 | 2015-07-16 | Synthon Biopharmaceuticals B.V. | Duocarmycin adcs showing improved in vivo antitumor activity |
MY177390A (en) * | 2014-01-10 | 2020-09-14 | Byondis Bv | Method for purifying cys-linked antibody-drug conjugates |
CA2935430C (en) | 2014-01-10 | 2018-09-18 | Synthon Biopharmaceuticals B.V. | Duocarmycin adcs for use in treatment of endometrial cancer |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1907014A1 (de) * | 1969-02-12 | 1970-09-03 | Haller Dr Wolfgang | Verfahren zur Trennung von Blutkomponenten,insbesondere von immunologisch aktiven Globulinen,von anderen Komponenten |
US4000098A (en) * | 1974-08-16 | 1976-12-28 | Palo Alto Medical Research Foundation | Separation of proteins by hydrophobic adsorption |
JPS5436201A (en) * | 1977-08-25 | 1979-03-16 | Microbial Chem Res Found | New oncostatic antibiotic auromomycin and its preparation |
FR2437213A1 (fr) * | 1978-09-28 | 1980-04-25 | Cm Ind | Produits cytotoxiques formes par liaison covalente de la chaine a de la ricine avec un anticorps et leur procede de preparation |
US4315851A (en) * | 1978-12-29 | 1982-02-16 | Kureha Kagaku Kogyo Kabushiki Kaisha | Pharmaceutical composition having antitumor activity |
US4276206A (en) * | 1979-01-18 | 1981-06-30 | Scripps Clinic & Research Foundation | Immunochemical conjugates: method and composition |
JPS5616418A (en) * | 1979-07-20 | 1981-02-17 | Teijin Ltd | Antitumor protein complex and its preparation |
US4355023A (en) * | 1980-09-30 | 1982-10-19 | The Massachusetts General Hospital | Antibody fragment compositions and process |
FR2504010B1 (fr) * | 1981-04-15 | 1985-10-25 | Sanofi Sa | Medicaments anticancereux contenant la chaine a de la ricine associee a un anticorps antimelanome et procede pour leur preparation |
FR2516794B1 (fr) * | 1981-11-20 | 1985-10-25 | Sanofi Sa | Nouveaux medicaments anticancereux pour le traitement des leucemies t constitues de la chaine a de la ricine et d'un anticorps monoclonal specifique |
US4461833A (en) * | 1982-06-23 | 1984-07-24 | University Patents, Inc. | Chromatographically purifying proteolytic procoagulant enzyme from animal tissue extract |
US4579941A (en) * | 1982-08-13 | 1986-04-01 | Mitsuitoatsu Chemicals, Inc. | Thermally-denatured deoxyribonucleic acid MD-011 and antitumor agent |
US4568488A (en) * | 1984-01-11 | 1986-02-04 | Lee Huang Sylvia | Reverse immunoaffinity chromatography purification method |
DE3426049A1 (de) * | 1984-07-14 | 1986-03-06 | Boehringer Ingelheim International GmbH, 6507 Ingelheim | Humaner-tumor-nekrose-faktor |
CA1286843C (en) * | 1985-10-30 | 1991-07-23 | Leo S. Lin | Purification method for proteins |
US4677197A (en) * | 1985-10-30 | 1987-06-30 | Cetus Corporation | Purification method for tumor necrosis factor |
US4689401A (en) * | 1986-03-06 | 1987-08-25 | Cetus Corporation | Method of recovering microbially produced recombinant ricin toxin a chain |
ATE169672T1 (de) * | 1986-03-07 | 1998-08-15 | Chiron Corp | Rekombinantes ricin-toxin |
-
1986
- 1986-10-10 US US06/917,469 patent/US4771128A/en not_active Expired - Fee Related
-
1987
- 1987-09-11 CA CA000546651A patent/CA1305415C/en not_active Expired - Fee Related
- 1987-10-01 NO NO874137A patent/NO874137L/no unknown
- 1987-10-09 AU AU79527/87A patent/AU611397B2/en not_active Expired - Fee Related
- 1987-10-09 FI FI874463A patent/FI874463A/fi not_active Application Discontinuation
- 1987-10-09 EP EP87114755A patent/EP0263526B1/en not_active Expired - Lifetime
- 1987-10-09 AT AT87114755T patent/ATE74137T1/de not_active IP Right Cessation
- 1987-10-09 DE DE8787114755T patent/DE3777744D1/de not_active Expired - Fee Related
- 1987-10-09 ES ES198787114755T patent/ES2031101T3/es not_active Expired - Lifetime
- 1987-10-09 DK DK530587A patent/DK530587A/da not_active Application Discontinuation
- 1987-10-12 JP JP62254796A patent/JPS63115900A/ja active Pending
-
1992
- 1992-03-26 GR GR910402197T patent/GR3004145T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
NO874137L (no) | 1988-04-11 |
DK530587D0 (da) | 1987-10-09 |
FI874463A0 (fi) | 1987-10-09 |
NO874137D0 (no) | 1987-10-01 |
FI874463A (fi) | 1988-04-11 |
CA1305415C (en) | 1992-07-21 |
US4771128A (en) | 1988-09-13 |
JPS63115900A (ja) | 1988-05-20 |
ATE74137T1 (de) | 1992-04-15 |
DK530587A (da) | 1988-04-11 |
AU7952787A (en) | 1988-04-14 |
GR3004145T3 (es) | 1993-03-31 |
AU611397B2 (en) | 1991-06-13 |
EP0263526B1 (en) | 1992-03-25 |
EP0263526A1 (en) | 1988-04-13 |
DE3777744D1 (de) | 1992-04-30 |
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