ATE72832T1 - Rekombinante plasmide, diese rekombinante plasmide enthaltende bakterien, verfahren zur herstellung von fermentierten nahrungsmitteln, tierfutter, bestandteile derseben oder proteinen unter verwendung dieser bakterien. - Google Patents

Rekombinante plasmide, diese rekombinante plasmide enthaltende bakterien, verfahren zur herstellung von fermentierten nahrungsmitteln, tierfutter, bestandteile derseben oder proteinen unter verwendung dieser bakterien.

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Publication number
ATE72832T1
ATE72832T1 AT85200316T AT85200316T ATE72832T1 AT E72832 T1 ATE72832 T1 AT E72832T1 AT 85200316 T AT85200316 T AT 85200316T AT 85200316 T AT85200316 T AT 85200316T AT E72832 T1 ATE72832 T1 AT E72832T1
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AT
Austria
Prior art keywords
gene
genes
recombinant plasmids
regie
subtilis
Prior art date
Application number
AT85200316T
Other languages
English (en)
Inventor
Jan Kok
Jan Maat
Der Vossen Josephus Maurit Van
Gerard Venema
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Application granted granted Critical
Publication of ATE72832T1 publication Critical patent/ATE72832T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Dairy Products (AREA)
AT85200316T 1984-03-02 1985-03-04 Rekombinante plasmide, diese rekombinante plasmide enthaltende bakterien, verfahren zur herstellung von fermentierten nahrungsmitteln, tierfutter, bestandteile derseben oder proteinen unter verwendung dieser bakterien. ATE72832T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8400687A NL8400687A (nl) 1984-03-02 1984-03-02 Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.
EP19850200316 EP0157441B1 (de) 1984-03-02 1985-03-04 Rekombinante Plasmide, diese rekombinante Plasmide enthaltende Bakterien, Verfahren zur Herstellung von fermentierten Nahrungsmitteln, Tierfutter, Bestandteile derseben oder Proteinen unter Verwendung dieser Bakterien

Publications (1)

Publication Number Publication Date
ATE72832T1 true ATE72832T1 (de) 1992-03-15

Family

ID=19843593

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85200316T ATE72832T1 (de) 1984-03-02 1985-03-04 Rekombinante plasmide, diese rekombinante plasmide enthaltende bakterien, verfahren zur herstellung von fermentierten nahrungsmitteln, tierfutter, bestandteile derseben oder proteinen unter verwendung dieser bakterien.

Country Status (7)

Country Link
EP (1) EP0157441B1 (de)
JP (1) JP2711258B2 (de)
AT (1) ATE72832T1 (de)
DE (1) DE3585415D1 (de)
DK (1) DK173773B1 (de)
NL (1) NL8400687A (de)
WO (1) WO1985003945A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8503316A (nl) * 1985-11-29 1987-06-16 Stichting Nl I Zuivelonderzoek Werkwijze voor het bereiden van proteinen met behulp van gastheer-organismen, in het bijzonder melkzuurbacterien, welke recombinant dna-plasmiden met een hoge replicon-aktiviteit en een hoge promotor-aktiviteit voor het betreffende proteine-coderende gen bevatten, de van dergelijke recombinant dna-plasmiden voorziene gastheerorganismen, de recombinant dna-plasmiden zelve, de replicon-aktiviteit en promotor-aktiviteit coderende dna-fragmenten, alsmede de verkregen proteinen.
ES2061482T3 (es) * 1986-05-02 1994-12-16 Gist Brocades Nv Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
NL8700767A (nl) * 1987-04-01 1988-11-01 Groningen Science Park Dipeptidase, isolering daarvan uit melkzuurbacterien, antilichamen tegen het dipeptidase, gebruik van het dipeptidase en van de antilichamen daartegen.
NL8801529A (nl) * 1988-06-15 1990-01-02 Nl Zuivelonderzoek Inst Werkwijze voor het selecteren en stabiel handhaven van recombinant dna in melkzuurbacterien.
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US5242821A (en) * 1989-07-10 1993-09-07 Valio, Finnish Co-Operative Dairies' Association Lactococcus promoter and signal sequences for expression in bacteria
NL8902010A (nl) * 1989-08-04 1991-03-01 Nl Zuivelonderzoek Inst Dna-fragment met ten minste een protease-gen, een dergelijk dna-fragment bevattende cloneringsvektor, een gastheercel getransformeerd met een dusdanig opgebouwde cloneringsvektor, alsmede de met dergelijke gastheercellen geproduceerde proteasen resp. bereide produkten zoals voedingsmiddelen.
GB9006400D0 (en) 1990-03-22 1990-05-23 Ciba Geigy Ag Bacterial vectors
EP0487159A1 (de) * 1990-11-23 1992-05-27 Unilever N.V. Für die Transformation von lebensmittelnsicheren Wirtszellen geeigneter Vektor, seine Verwendung für solche Transformationen und Verwendung der transformierten Zellen in Biotransformationsprozessen
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
EP0972838B1 (de) * 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
IL66065A (en) * 1981-06-22 1989-06-30 Lilly Co Eli Recombinant dna cloning vectors and the e.coli transformants thereof
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos

Also Published As

Publication number Publication date
DK504585D0 (da) 1985-11-01
JP2711258B2 (ja) 1998-02-10
JPS62500280A (ja) 1987-02-05
WO1985003945A1 (en) 1985-09-12
DE3585415D1 (de) 1992-04-02
EP0157441B1 (de) 1992-02-26
DK504585A (da) 1985-11-01
EP0157441A1 (de) 1985-10-09
DK173773B1 (da) 2001-10-01
NL8400687A (nl) 1985-10-01

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