ATE530641T1 - Modulation von enzymaktivität durch änderung reziproker stereogeometrischer positionen zweier katalytischer zentren in einem enzym - Google Patents

Modulation von enzymaktivität durch änderung reziproker stereogeometrischer positionen zweier katalytischer zentren in einem enzym

Info

Publication number
ATE530641T1
ATE530641T1 AT04811921T AT04811921T ATE530641T1 AT E530641 T1 ATE530641 T1 AT E530641T1 AT 04811921 T AT04811921 T AT 04811921T AT 04811921 T AT04811921 T AT 04811921T AT E530641 T1 ATE530641 T1 AT E530641T1
Authority
AT
Austria
Prior art keywords
enzyme
dna
cleaving
duplex
cleavage
Prior art date
Application number
AT04811921T
Other languages
English (en)
Inventor
Chudi Guan
Sanjay Kumar
Rebecca Kucera
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of ATE530641T1 publication Critical patent/ATE530641T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
AT04811921T 2003-11-21 2004-11-22 Modulation von enzymaktivität durch änderung reziproker stereogeometrischer positionen zweier katalytischer zentren in einem enzym ATE530641T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52412303P 2003-11-21 2003-11-21
PCT/US2004/039288 WO2005052124A2 (en) 2003-11-21 2004-11-22 Modified dna cleaving enzymes and methods of use

Publications (1)

Publication Number Publication Date
ATE530641T1 true ATE530641T1 (de) 2011-11-15

Family

ID=34632867

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04811921T ATE530641T1 (de) 2003-11-21 2004-11-22 Modulation von enzymaktivität durch änderung reziproker stereogeometrischer positionen zweier katalytischer zentren in einem enzym

Country Status (4)

Country Link
EP (1) EP1702063B1 (de)
CN (1) CN1906292B (de)
AT (1) ATE530641T1 (de)
WO (1) WO2005052124A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007120627A2 (en) 2006-04-11 2007-10-25 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
CN102301009B (zh) * 2009-02-03 2015-09-30 新英格兰生物实验室公司 使用酶在dna中产生随机双链断裂
CN102766611B (zh) * 2012-07-12 2014-02-12 昆明理工大学 硫化叶菌病毒脱氧尿苷焦磷酸酶和编码这种酶的多核苷酸
CN104195122B (zh) * 2014-05-26 2017-01-04 杭州师范大学 Yep非特异性核酸酶、基因、载体、工程菌及应用
CN104306960A (zh) * 2014-09-24 2015-01-28 郭和友 一种利用切割酶治疗病毒及癌症方法
CN104306990A (zh) * 2014-09-24 2015-01-28 郭和友 一种利用切割酶治疗病毒性肝病及癌症方法
CN104306989A (zh) * 2014-09-27 2015-01-28 郭和友 一种利用切割酶治疗细菌方法
CN104306962A (zh) * 2014-10-05 2015-01-28 郭和友 利用切割酶切割病毒转录酶方法
CN107217308A (zh) * 2017-06-21 2017-09-29 北京贝瑞和康生物技术股份有限公司 一种用于检测染色体拷贝数变异的测序文库构建方法和试剂盒
EP4083228A1 (de) * 2017-06-28 2022-11-02 New England Biolabs, Inc. Verfahren zur entfernung und/oder zum nachweis von nukleinsäuren mit fehlangepassten nukleotiden
CN114807086B (zh) * 2022-04-15 2024-02-20 杭州师范大学 一种霍利迪连接体解离酶DrRuvC及其编码基因和应用
CN116640743B (zh) * 2023-07-24 2023-11-07 北京志道生物科技有限公司 一种核酸内切酶及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5604098A (en) * 1993-03-24 1997-02-18 Molecular Biology Resources, Inc. Methods and materials for restriction endonuclease applications
US6340566B1 (en) * 2000-03-28 2002-01-22 The Regents Of The University Of California Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches

Also Published As

Publication number Publication date
CN1906292B (zh) 2013-03-27
WO2005052124A3 (en) 2005-10-27
WO2005052124A2 (en) 2005-06-09
CN1906292A (zh) 2007-01-31
EP1702063A4 (de) 2007-08-01
EP1702063A2 (de) 2006-09-20
EP1702063B1 (de) 2011-10-26

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