WO2004024864A3 - Rna-cleaving dna enzymes with altered regio- or enantioselectivity - Google Patents

Rna-cleaving dna enzymes with altered regio- or enantioselectivity Download PDF

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Publication number
WO2004024864A3
WO2004024864A3 PCT/US2003/027307 US0327307W WO2004024864A3 WO 2004024864 A3 WO2004024864 A3 WO 2004024864A3 US 0327307 W US0327307 W US 0327307W WO 2004024864 A3 WO2004024864 A3 WO 2004024864A3
Authority
WO
WIPO (PCT)
Prior art keywords
enantioselectivity
rna
stranded
dna enzymes
cleaving dna
Prior art date
Application number
PCT/US2003/027307
Other languages
French (fr)
Other versions
WO2004024864A2 (en
Inventor
Gerald F Joyce
Phillip T Ordoukhanian
Original Assignee
Scripps Research Inst
Gerald F Joyce
Phillip T Ordoukhanian
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst, Gerald F Joyce, Phillip T Ordoukhanian filed Critical Scripps Research Inst
Priority to AU2003268332A priority Critical patent/AU2003268332A1/en
Publication of WO2004024864A2 publication Critical patent/WO2004024864A2/en
Publication of WO2004024864A3 publication Critical patent/WO2004024864A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/319—Chemical structure of the backbone linked by 2'-5' linkages, i.e. having a free 3'-position

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides catalytic single-stranded DNA molecules, and methods using the same, that have site-specific endonuclease activity that is specific for a cleavage site in a substrate nucleic acid sequence, that includes a non-naturally occurring single-stranded ribonucleic acid. The catalytic DNA molecule includes one or more loop regions and one or more binding regions, wherein the binding regions bind to complementary sequences of the substrate nucleic acid sequence. These non-naturally occurring single-stranded nucleic acids include a 2',5' linked residue or an L-enantiomer residue.
PCT/US2003/027307 2002-09-16 2003-08-29 Rna-cleaving dna enzymes with altered regio- or enantioselectivity WO2004024864A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003268332A AU2003268332A1 (en) 2002-09-16 2003-08-29 Rna-cleaving dna enzymes with altered regio- or enantioselectivity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41097302P 2002-09-16 2002-09-16
US60/410,973 2002-09-16

Publications (2)

Publication Number Publication Date
WO2004024864A2 WO2004024864A2 (en) 2004-03-25
WO2004024864A3 true WO2004024864A3 (en) 2005-01-20

Family

ID=31994227

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/027307 WO2004024864A2 (en) 2002-09-16 2003-08-29 Rna-cleaving dna enzymes with altered regio- or enantioselectivity

Country Status (3)

Country Link
AU (1) AU2003268332A1 (en)
TW (1) TW200413528A (en)
WO (1) WO2004024864A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113278616B (en) * 2021-06-17 2022-07-01 温州医科大学 DNAzyme capable of specifically recognizing toxoplasma gondii and having RNA cutting function and kit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020102694A1 (en) * 2000-03-31 2002-08-01 Ronald Breaker Nucleozymes with endonuclease activity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020102694A1 (en) * 2000-03-31 2002-08-01 Ronald Breaker Nucleozymes with endonuclease activity

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SHIH ET AL.: "Ribozyme cleavage of a 2',5'-phosphodiester linkage: Mechanism and a restricted divalent metal-ion requirement", RNA, vol. 5, no. 9, 1999, pages 1140 - 1148, XP002903957 *

Also Published As

Publication number Publication date
WO2004024864A2 (en) 2004-03-25
AU2003268332A1 (en) 2004-04-30
TW200413528A (en) 2004-08-01
AU2003268332A8 (en) 2004-04-30

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