ATE51251T1 - Verfahren und reagenzien zum nachweis von normalerweise einkettigen nukleinsaeuren, insbesondere rns, in einem biologischen material, insbesondere in einem praeparat von leukocyten. - Google Patents

Verfahren und reagenzien zum nachweis von normalerweise einkettigen nukleinsaeuren, insbesondere rns, in einem biologischen material, insbesondere in einem praeparat von leukocyten.

Info

Publication number
ATE51251T1
ATE51251T1 AT84401974T AT84401974T ATE51251T1 AT E51251 T1 ATE51251 T1 AT E51251T1 AT 84401974 T AT84401974 T AT 84401974T AT 84401974 T AT84401974 T AT 84401974T AT E51251 T1 ATE51251 T1 AT E51251T1
Authority
AT
Austria
Prior art keywords
rna
monocatenary
determined
nucleic acid
probe
Prior art date
Application number
AT84401974T
Other languages
English (en)
Inventor
Michel Crepin
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Application granted granted Critical
Publication of ATE51251T1 publication Critical patent/ATE51251T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y10S436/813—Cancer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/825—Pretreatment for removal of interfering factors from sample
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
AT84401974T 1983-10-03 1984-10-03 Verfahren und reagenzien zum nachweis von normalerweise einkettigen nukleinsaeuren, insbesondere rns, in einem biologischen material, insbesondere in einem praeparat von leukocyten. ATE51251T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8315726A FR2552879B1 (fr) 1983-10-03 1983-10-03 Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes
EP84401974A EP0140764B1 (de) 1983-10-03 1984-10-03 Verfahren und Reagenzien zum Nachweis von normalerweise einkettigen Nukleinsäuren, insbesondere RNS, in einem biologischen Material, insbesondere in einem Präparat von Leukocyten

Publications (1)

Publication Number Publication Date
ATE51251T1 true ATE51251T1 (de) 1990-04-15

Family

ID=9292760

Family Applications (1)

Application Number Title Priority Date Filing Date
AT84401974T ATE51251T1 (de) 1983-10-03 1984-10-03 Verfahren und reagenzien zum nachweis von normalerweise einkettigen nukleinsaeuren, insbesondere rns, in einem biologischen material, insbesondere in einem praeparat von leukocyten.

Country Status (6)

Country Link
US (1) US5070009A (de)
EP (1) EP0140764B1 (de)
JP (1) JPH0653080B2 (de)
AT (1) ATE51251T1 (de)
DE (1) DE3481717D1 (de)
FR (1) FR2552879B1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU619170B2 (en) * 1987-01-09 1992-01-23 Abbott Laboratories Diagnostic assays using nucleic acid probes
EP0309558B1 (de) * 1987-04-15 1994-11-02 Yissum Research Development Company Of The Hebrew University Of Jerusalem Verfahren zur feststellung der expression bioregulatorischer gene in mikrokulturen eukariontischer zellen
US5614551A (en) * 1994-01-24 1997-03-25 The Johns Hopkins University Inhibitors of fatty acid synthesis as antimicrobial agents
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2152477A1 (en) * 1971-09-17 1973-04-27 Merieux Inst Interferon-inducers - by extraction of cells inoculated with rna virus
US4038143A (en) * 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
FR2505845A1 (fr) * 1981-05-13 1982-11-19 Choay Sa Procede de dosage de la 2',5'-oligo-adenylate-synthetase, methodes de detection d'une infection virale ou microbienne, de dosage de la concentration de l'interferon circulant ou present dans des tissus, apres administration de l'interferon, ou apres administration d'inducteurs de l'interferon et ensemble pret a l'emploi, ou kit, pour la mise en oeuvre desdits procedes de dosage et methodes de detection et de dosage
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
FR2518755B1 (fr) * 1981-12-23 1986-04-11 Pasteur Institut Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material

Also Published As

Publication number Publication date
FR2552879B1 (fr) 1987-08-14
JPH0653080B2 (ja) 1994-07-20
EP0140764A1 (de) 1985-05-08
DE3481717D1 (de) 1990-04-26
EP0140764B1 (de) 1990-03-21
JPS60102200A (ja) 1985-06-06
US5070009A (en) 1991-12-03
FR2552879A1 (fr) 1985-04-05

Similar Documents

Publication Publication Date Title
Burridge et al. Purification and structural analysis of myosins from brain and other non-muscle tissues
Rosenthal et al. Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes
EP0442026B1 (de) Verfahren zur Trennung und Reinigung menschlicher, genomischer DNS
Wake et al. Analysis of casein fractions by zone electrophoresis in concentrated urea
US5484701A (en) Method for sequencing DNA using biotin-strepavidin conjugates to facilitate the purification of primer extension products
JP2552691B2 (ja) 生物学的試料中の核酸評価のためのカオトロピック方法
Kaplan et al. rRNA accumulation and protein synthetic patterns in growing mouse oocytes
ATE250073T1 (de) Verfahren und vorrichtung zur simultanen isolierung von genomischer dns und hochreiner total rns
JPH08506340A (ja) 第４級アミン界面活性剤及びｒｎａの単離におけるその利用法
DE69031957T2 (de) Verfahren zur Extraktion, Amplifikation und zum Nachweis von Nukleinsäuren aus Vollblut
NO841725L (no) Fremgangsmaate og stoffer til bruk ved analyse og paavisning av genetisk materiale
CN105120986B (zh) 用于核酸纯化的一步程序
JP2002511583A (ja) 生物学的サンプルの電気溶出のための方法及び実施装置
Hnilica et al. Fractionation and analysis of nuclear proteins in sea urchin embryos
JP2847755B2 (ja) 酵素的精製方法
ATE51251T1 (de) Verfahren und reagenzien zum nachweis von normalerweise einkettigen nukleinsaeuren, insbesondere rns, in einem biologischen material, insbesondere in einem praeparat von leukocyten.
CA2279131A1 (en) Electrophoretic separation of nucleic acids from proteins at low ph
Poccia et al. Nuclei and chromosomal proteins
JPS5948081A (ja) Ｒｎａブロツト紙
WO2001016170A3 (en) Card proteins involved in cell death regulation
Müller et al. Species-specific aggregation factor in sponges: IV. Inactivation of the aggregation factor by mucoid cells from another species
US4775631A (en) Method of localizing nucleic acids bound to polyamide supports
US4782027A (en) Protein detection by negative staining
JP4735926B2 (ja) 核酸の高効率回収方法
Briggs et al. Nuclear localization of lactoferrin in the human granulocyte: Artifact incurred during slide preparation.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee