ATE508200T1 - SEQUENCING THROUGH INCORPORATION - Google Patents

SEQUENCING THROUGH INCORPORATION

Info

Publication number
ATE508200T1
ATE508200T1 AT00913566T AT00913566T ATE508200T1 AT E508200 T1 ATE508200 T1 AT E508200T1 AT 00913566 T AT00913566 T AT 00913566T AT 00913566 T AT00913566 T AT 00913566T AT E508200 T1 ATE508200 T1 AT E508200T1
Authority
AT
Austria
Prior art keywords
microfluidic
arrays
flowable
particle sets
sequencing
Prior art date
Application number
AT00913566T
Other languages
German (de)
Inventor
J Parce
Theo Nikiforov
Mehta Tammy Burd
Anne Kopf-Sill
Andrea Chow
Michael Knapp
Original Assignee
Caliper Life Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27382592&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE508200(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Caliper Life Sciences Inc filed Critical Caliper Life Sciences Inc
Application granted granted Critical
Publication of ATE508200T1 publication Critical patent/ATE508200T1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00468—Beads by manipulation of individual beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00564—Handling or washing solid phase elements, e.g. beads
    • G01N2035/00574—Means for distributing beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members. The application particularly focusses on methods of performing a plurality of chemical reactions, for instance nucleic acid synthesis and sequencing reactions, in said microfluidic devices.
AT00913566T 1999-02-23 2000-02-22 SEQUENCING THROUGH INCORPORATION ATE508200T1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12122399P 1999-02-23 1999-02-23
US12782599P 1999-04-05 1999-04-05
US12864399P 1999-04-09 1999-04-09
PCT/US2000/004486 WO2000050642A1 (en) 1999-02-23 2000-02-22 Sequencing by incorporation

Publications (1)

Publication Number Publication Date
ATE508200T1 true ATE508200T1 (en) 2011-05-15

Family

ID=27382592

Family Applications (3)

Application Number Title Priority Date Filing Date
AT09015930T ATE556149T1 (en) 1999-02-23 2000-02-22 MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS
AT00913566T ATE508200T1 (en) 1999-02-23 2000-02-22 SEQUENCING THROUGH INCORPORATION
AT00911913T ATE469699T1 (en) 1999-02-23 2000-02-22 MANIPULATION OF MICROPARTICLES IN MICROFLUID SYSTEMS

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT09015930T ATE556149T1 (en) 1999-02-23 2000-02-22 MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT00911913T ATE469699T1 (en) 1999-02-23 2000-02-22 MANIPULATION OF MICROPARTICLES IN MICROFLUID SYSTEMS

Country Status (6)

Country Link
US (10) US6613513B1 (en)
EP (3) EP1163369B1 (en)
AT (3) ATE556149T1 (en)
AU (2) AU3372800A (en)
DE (2) DE60044490D1 (en)
WO (2) WO2000050642A1 (en)

Families Citing this family (776)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
DE69825601T2 (en) 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline METHOD FOR THE ANALYSIS OF POLYMERS
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
ATE556149T1 (en) * 1999-02-23 2012-05-15 Caliper Life Sciences Inc MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
EP1179087B1 (en) 1999-05-17 2019-03-27 Caliper Life Sciences, Inc. Focusing of microparticles in microfluidic systems
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US8481268B2 (en) * 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6858185B1 (en) 1999-08-25 2005-02-22 Caliper Life Sciences, Inc. Dilutions in high throughput systems with a single vacuum source
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
CA2290731A1 (en) 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
CA2399199A1 (en) * 2000-02-23 2001-08-30 Ring-Ling Chien Multi-reservoir pressure control system
US20020012971A1 (en) 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
DE10015448A1 (en) * 2000-03-29 2001-10-11 November Ag Molekulare Medizin Detection and quantification of binding between affinity molecules useful in screening for drugs, does not require removal of reaction solution prior to measurement
EP1182267B1 (en) * 2000-03-30 2012-01-18 Toyota Jidosha Kabushiki Kaisha Method of determining base sequence of single nucleic acid molecule
US6734420B2 (en) 2000-04-06 2004-05-11 Quantum Dot Corporation Differentiable spectral bar code methods and systems
DE60023464T2 (en) * 2000-06-05 2006-07-20 Stmicroelectronics S.R.L., Agrate Brianza Process for the production of integrated chemical microreactors made of semiconductor material and integrated microreactor
US9709559B2 (en) * 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
AU2001273057A1 (en) * 2000-06-27 2002-01-08 Fluidigm Corporation A microfluidic design automation method and system
US6720187B2 (en) 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
GB0022069D0 (en) * 2000-09-08 2000-10-25 Pyrosequencing Ab Method
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP3034627B1 (en) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massive parallel method for decoding dna and rna
EP1336097A4 (en) * 2000-10-13 2006-02-01 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
US7105304B1 (en) 2000-11-07 2006-09-12 Caliper Life Sciences, Inc. Pressure-based mobility shift assays
US20090118139A1 (en) 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
WO2002038806A2 (en) * 2000-11-13 2002-05-16 Gnothis Holding Sa Detection of nucleic acid polymorphisms
FR2817343B1 (en) * 2000-11-29 2003-05-09 Commissariat Energie Atomique METHOD AND DEVICES FOR TRANSPORTING AND CONCENTRATING AN ANALYTE PRESENT IN A SAMPLE
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7691645B2 (en) * 2001-01-09 2010-04-06 Agilent Technologies, Inc. Immunosubtraction method
US20020110835A1 (en) * 2001-02-13 2002-08-15 Rajan Kumar Microfluidic devices and methods
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
CA2440754A1 (en) * 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US6812456B2 (en) 2001-03-19 2004-11-02 Gyros Ab Microfluidic system (EDI)
WO2002075312A1 (en) 2001-03-19 2002-09-26 Gyros Ab Characterization of reaction variables
US6717136B2 (en) 2001-03-19 2004-04-06 Gyros Ab Microfludic system (EDI)
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
FR2822848B1 (en) * 2001-03-28 2004-05-07 Genesystems INTEGRATED AND MINIATURE NUCLEIC ACID SEQUENCING DEVICE
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
WO2002082057A2 (en) * 2001-04-03 2002-10-17 Micronics, Inc. Split focusing cytometer
US20020164816A1 (en) * 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6727479B2 (en) * 2001-04-23 2004-04-27 Stmicroelectronics S.R.L. Integrated device based upon semiconductor technology, in particular chemical microreactor
DE10120797B4 (en) * 2001-04-27 2005-12-22 Genovoxx Gmbh Method for analyzing nucleic acid chains
DE10120798B4 (en) * 2001-04-27 2005-12-29 Genovoxx Gmbh Method for determining gene expression
US7172739B2 (en) 2001-05-22 2007-02-06 University Of Vermont And State Agricultural College Protein fractionation
US20020192714A1 (en) * 2001-05-22 2002-12-19 University Of Vermont And State Agricultural College Single polymer matrix unit chromatography
US7723123B1 (en) 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
MXPA03011558A (en) * 2001-06-12 2004-03-26 Wellstat Therapeutics Corp Compounds for the treatment of metabolic disorders.
GB0114849D0 (en) * 2001-06-18 2001-08-08 Pig Improvement Co Uk Ltd System
WO2003000404A1 (en) * 2001-06-22 2003-01-03 Domantis Limited Matrix screening method
AU2002319668A1 (en) * 2001-07-27 2003-02-17 President And Fellows Of Harvard College Laminar mixing apparatus and methods
WO2003016547A2 (en) * 2001-08-13 2003-02-27 Vanderbilt University Distribution of solutions across a surface
US7666661B2 (en) 2001-08-27 2010-02-23 Platypus Technologies, Llc Substrates, devices, and methods for quantitative liquid crystal assays
US7189368B2 (en) 2001-09-17 2007-03-13 Gyros Patent Ab Functional unit enabling controlled flow in a microfluidic device
JP4368681B2 (en) * 2001-09-17 2009-11-18 ユィロス・パテント・アクチボラグ Functional unit that enables control of flow in microfluidic devices
US6907895B2 (en) * 2001-09-19 2005-06-21 The United States Of America As Represented By The Secretary Of Commerce Method for microfluidic flow manipulation
US6803568B2 (en) * 2001-09-19 2004-10-12 Predicant Biosciences, Inc. Multi-channel microfluidic chip for electrospray ionization
US7238477B2 (en) * 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
US6982165B2 (en) * 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US20030072682A1 (en) * 2001-10-11 2003-04-17 Dan Kikinis Method and apparatus for performing biochemical testing in a microenvironment
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
SE526185C2 (en) * 2001-11-07 2005-07-19 Prolight Diagnostics Ab Method and apparatus for immunoassay
WO2003040395A2 (en) 2001-11-07 2003-05-15 Applera Corporation Universal nucleotides for nucleic acid analysis
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7473361B2 (en) * 2001-11-30 2009-01-06 Cornell Research Foundation Diffusion-based molecular separation in structured microfluidic devices
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20030109067A1 (en) 2001-12-06 2003-06-12 Immunetech, Inc. Homogeneous immunoassays for multiple allergens
WO2003054517A2 (en) * 2001-12-19 2003-07-03 Biogen Idec Ma Inc. Methods for detecting half-antibodies using chip-based gel electrophoresis
US7105810B2 (en) 2001-12-21 2006-09-12 Cornell Research Foundation, Inc. Electrospray emitter for microfluidic channel
US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
US20050118647A1 (en) * 2001-12-31 2005-06-02 Yung-Chiang Chung Microfluidic mixer apparatus and microfluidic reactor apparatus for microfluidic processing
US7189370B2 (en) * 2002-02-11 2007-03-13 Microchem Solutions Apparatus and methods for high throughput and high-resolution assays
JP2003232791A (en) * 2002-02-12 2003-08-22 Olympus Optical Co Ltd Probe solid-phase reaction array
AU2003219775A1 (en) * 2002-02-15 2003-09-09 President And Fellows Of Harvard College Fluidics systems including magnetic or electric fields and methods of using the same
US7303727B1 (en) 2002-03-06 2007-12-04 Caliper Life Sciences, Inc Microfluidic sample delivery devices, systems, and methods
US7560267B2 (en) * 2002-03-18 2009-07-14 City University Of Hong Kong Apparatus and methods for on-chip monitoring of cellular reactions
DE10214395A1 (en) * 2002-03-30 2003-10-23 Dmitri Tcherkassov Parallel sequencing of nucleic acid segments, useful for detecting single-nucleotide polymorphisms, by single-base extensions with labeled nucleotide
WO2003085379A2 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
ATE304610T1 (en) * 2002-04-04 2005-09-15 Biotage Ab PRIMER EXTENSION BASED METHOD USING NUCLEOTIDES LABELED BY CLIVABLE LINKERS
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
US6808075B2 (en) 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US8697029B2 (en) * 2002-04-18 2014-04-15 The Regents Of The University Of Michigan Modulated physical and chemical sensors
WO2005047863A2 (en) * 2003-11-10 2005-05-26 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
ATE378424T1 (en) * 2002-08-12 2007-11-15 Gnothis Holding Sa DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS BY SINGLE MOLECULE ANALYSIS
US6916455B2 (en) * 2002-08-14 2005-07-12 The Regents Of The University Of California Protein crystallography prescreen kit
US7901630B2 (en) 2002-08-20 2011-03-08 Illumina, Inc. Diffraction grating-based encoded microparticle assay stick
US7900836B2 (en) 2002-08-20 2011-03-08 Illumina, Inc. Optical reader system for substrates having an optically readable code
US7923260B2 (en) 2002-08-20 2011-04-12 Illumina, Inc. Method of reading encoded particles
US7164533B2 (en) 2003-01-22 2007-01-16 Cyvera Corporation Hybrid random bead/chip based microarray
US7872804B2 (en) 2002-08-20 2011-01-18 Illumina, Inc. Encoded particle having a grating with variations in the refractive index
US7508608B2 (en) 2004-11-17 2009-03-24 Illumina, Inc. Lithographically fabricated holographic optical identification element
ES2407681T3 (en) 2002-08-23 2013-06-13 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing.
DK3147292T3 (en) 2002-08-23 2018-12-03 Illumina Cambridge Ltd LABELED NUCLEOTIDES
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US20100255603A9 (en) 2002-09-12 2010-10-07 Putnam Martin A Method and apparatus for aligning microbeads in order to interrogate the same
US7092160B2 (en) 2002-09-12 2006-08-15 Illumina, Inc. Method of manufacturing of diffraction grating-based optical identification element
US6881039B2 (en) * 2002-09-23 2005-04-19 Cooligy, Inc. Micro-fabricated electrokinetic pump
US7143785B2 (en) 2002-09-25 2006-12-05 California Institute Of Technology Microfluidic large scale integration
ES2375724T3 (en) 2002-09-27 2012-03-05 The General Hospital Corporation MICROFLUDE DEVICE FOR SEPERATION OF CELLS AND ITS USES.
AU2003265185A1 (en) * 2002-09-27 2004-04-19 Biotage Ab New sequencing method for sequencing rna molecules
JP5695287B2 (en) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー Nucleic acid analysis of microfluids
JP2006522463A (en) 2002-11-01 2006-09-28 クーリギー インコーポレイテッド Optimal spreader system, apparatus and method for micro heat exchange cooled by fluid
US7836597B2 (en) 2002-11-01 2010-11-23 Cooligy Inc. Method of fabricating high surface to volume ratio structures and their integration in microheat exchangers for liquid cooling system
US20060211055A1 (en) * 2002-11-12 2006-09-21 Caliper Life Sciences, Inc. Capture and release assay system and method
EP1420251B1 (en) * 2002-11-12 2013-01-09 Panasonic Corporation Specific coupling reaction measuring method
US7175810B2 (en) * 2002-11-15 2007-02-13 Eksigent Technologies Processing of particles
ATE459725T1 (en) * 2002-11-18 2010-03-15 Panomics Inc CAGED SENSORS, REGULATORS AND CONNECTIONS AND USES THEREOF
CA2506935A1 (en) * 2002-11-20 2004-06-03 University Of Virginia Patent Foundation Isolation of sperm cells from other biological materials using microfabricated devices and related methods thereof
ATE462017T1 (en) * 2002-12-02 2010-04-15 Illumina Cambridge Ltd DETERMINATION OF METHYLATION OF NUCLEIC ACID SEQUENCES
US7932025B2 (en) * 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
WO2004061085A2 (en) 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
US7332129B2 (en) 2003-01-09 2008-02-19 3M Innovative Properties Company Sample processing device having process chambers with bypass slots
SE0300454D0 (en) * 2003-02-19 2003-02-19 Aamic Ab Nozzles for electrospray ionization and methods of fabricating them
US8399196B2 (en) 2003-02-21 2013-03-19 Geneform Technologies Limited Nucleic acid sequencing methods, kits and reagents
SE0300822D0 (en) * 2003-03-23 2003-03-23 Gyros Ab A collection of Micro Scale Devices
AU2004228678A1 (en) * 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7476363B2 (en) * 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7007710B2 (en) * 2003-04-21 2006-03-07 Predicant Biosciences, Inc. Microfluidic devices and methods
WO2004101743A2 (en) * 2003-05-06 2004-11-25 Bellbrook Labs, Llc Three dimensional cell cultures in a microscale fluid handling system
WO2005007264A2 (en) * 2003-07-14 2005-01-27 Waters Investments Limited Separation device with integral guard column
US7021369B2 (en) * 2003-07-23 2006-04-04 Cooligy, Inc. Hermetic closed loop fluid system
US7591302B1 (en) 2003-07-23 2009-09-22 Cooligy Inc. Pump and fan control concepts in a cooling system
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US7413712B2 (en) * 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
GB0320337D0 (en) * 2003-08-29 2003-10-01 Syrris Ltd A microfluidic system
US20050136439A1 (en) * 2003-09-12 2005-06-23 North Carolina State University Novel methods of inorganic compound discovery and synthesis
US7537807B2 (en) 2003-09-26 2009-05-26 Cornell University Scanned source oriented nanofiber formation
US20060134644A1 (en) * 2003-10-28 2006-06-22 Dakota Technologies, Inc. Apparatus and methods for detecting target analyte
EP1725572B1 (en) 2003-11-05 2017-05-31 AGCT GmbH Macromolecular nucleotide compounds and methods for using the same
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP4041458B2 (en) * 2003-12-12 2008-01-30 日立ソフトウエアエンジニアリング株式会社 Fluorescent bead detection method and fluorescent bead detection device
KR20070037432A (en) 2004-01-15 2007-04-04 도꾸리쯔교세이호징 가가꾸 기쥬쯔 신꼬 기꼬 Chemical analysis apparatus and method of chemical analysis
JP3991034B2 (en) * 2004-01-23 2007-10-17 キヤノン株式会社 Detection method
SE0400181D0 (en) * 2004-01-29 2004-01-29 Gyros Ab Segmented porous and preloaded microscale devices
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
US7433123B2 (en) 2004-02-19 2008-10-07 Illumina, Inc. Optical identification element having non-waveguide photosensitive substrate with diffraction grating therein
EP2436778A3 (en) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
AU2005218622A1 (en) * 2004-03-03 2005-09-15 Living Microsystems Magnetic device for isolation of cells and biomolecules in a microfluidic environment
US7442339B2 (en) * 2004-03-31 2008-10-28 Intel Corporation Microfluidic apparatus, Raman spectroscopy systems, and methods for performing molecular reactions
US9863941B2 (en) * 2004-04-01 2018-01-09 Nanyang Technological University Microchip and method for detecting molecules and molecular interactions
RU2252411C1 (en) * 2004-04-09 2005-05-20 Общество с ограниченной ответственностью "Институт рентгеновской оптики" Fluorescent sensor on basis of multichannel structures
US7297246B2 (en) * 2004-04-22 2007-11-20 Sandia Corporation Electrokinetic pump
WO2005108620A2 (en) 2004-05-03 2005-11-17 Handylab, Inc. Processing polynucleotide-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
CN102680440A (en) 2004-06-07 2012-09-19 先锋生物科技股份有限公司 Optical lens system and method for microfluidic devices
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
EP1611955A1 (en) * 2004-07-01 2006-01-04 Agilent Technologies, Inc. Microfluidic chip assembly with filtering channel
JP2006029798A (en) * 2004-07-12 2006-02-02 Hitachi Software Eng Co Ltd High reaction efficiency bio-substance inspection chip having built-in reagent
US8124032B2 (en) * 2004-07-16 2012-02-28 Simon Fraser University Microfluidic device and method of using same
WO2006020363A2 (en) 2004-07-21 2006-02-23 Illumina, Inc. Method and apparatus for drug product tracking using encoded optical identification elements
US20060022130A1 (en) * 2004-07-29 2006-02-02 Predicant Biosciences, Inc., A Delaware Corporation Microfluidic devices and methods with integrated electrical contact
WO2006098752A2 (en) * 2004-07-29 2006-09-21 Kim Laboratories Ultrasensitive sensor and rapid detection of analytes
JP4543312B2 (en) * 2004-08-10 2010-09-15 横河電機株式会社 Microreactor
DE102004044048A1 (en) * 2004-09-09 2006-03-30 Qualis Laboratorium Gmbh Biochemical characterization of objects via reaction products from cellular systems, especially identifying pyrogens using blood as indicator, by enzymatic assay including immobilization on magnetic particles
EP1794581A2 (en) 2004-09-15 2007-06-13 Microchip Biotechnologies, Inc. Microfluidic devices
US20060060769A1 (en) * 2004-09-21 2006-03-23 Predicant Biosciences, Inc. Electrospray apparatus with an integrated electrode
US7591883B2 (en) 2004-09-27 2009-09-22 Cornell Research Foundation, Inc. Microfiber supported nanofiber membrane
JP4263154B2 (en) * 2004-09-30 2009-05-13 日立ソフトウエアエンジニアリング株式会社 Functional fine particle array and method of using the same
DE102004050575B3 (en) * 2004-10-15 2006-01-05 Siemens Ag Method for the combined isolation of magnetic beads from a liquid sample and subsequent thermal cycling for the PCR and associated assembly
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
DE602005019791D1 (en) 2004-11-16 2010-04-15 Illumina Inc METHOD AND DEVICE FOR READING CODED MICROBALLS
US20060134772A1 (en) * 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
KR100695134B1 (en) * 2004-11-25 2007-03-14 삼성전자주식회사 Microarray using laminar flow and preparation method thereof
GB0426082D0 (en) * 2004-11-26 2004-12-29 Norchip As A device for carrying out biological assays
US9260693B2 (en) 2004-12-03 2016-02-16 Cytonome/St, Llc Actuation of parallel microfluidic arrays
WO2006074351A2 (en) * 2005-01-05 2006-07-13 Agencourt Personal Genomics Reversible nucleotide terminators and uses thereof
KR100682920B1 (en) * 2005-01-20 2007-02-15 삼성전자주식회사 Microfluidic chip for multiple bioassay and its method for production
EP1841854A4 (en) * 2005-01-27 2009-10-21 Applera Corp Sample preparation devices and methods
CN101146595B (en) 2005-01-28 2012-07-04 杜克大学 Apparatuses and methods for manipulating droplets on a printed circuit board
CA2593855A1 (en) * 2005-01-31 2006-08-10 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
AU2006210553A1 (en) 2005-02-01 2006-08-10 Ab Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
EP2233582A1 (en) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
US9040237B2 (en) * 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US9695472B2 (en) * 2005-03-04 2017-07-04 Intel Corporation Sensor arrays and nucleic acid sequencing applications
JP4626350B2 (en) * 2005-03-22 2011-02-09 ソニー株式会社 A flow path system having a reaction part suitable for hybridization detection, and a hybridization detection apparatus using the flow path system
KR101260400B1 (en) * 2005-04-01 2013-05-09 미쓰비시 가가쿠 메디엔스 가부시키가이샤 Biosample Multiple Autoanalyzer, Method of Autoanalysis and Reaction Cuvette
US9097723B2 (en) * 2005-04-01 2015-08-04 Caliper Life Sciences, Inc. Method and apparatus for performing peptide digestion on a microfluidic device
US20060223178A1 (en) * 2005-04-05 2006-10-05 Tom Barber Devices and methods for magnetic enrichment of cells and other particles
WO2006108087A2 (en) * 2005-04-05 2006-10-12 Cellpoint Diagnostics Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026418A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070196820A1 (en) * 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US20060246576A1 (en) * 2005-04-06 2006-11-02 Affymetrix, Inc. Fluidic system and method for processing biological microarrays in personal instrumentation
JP2006300548A (en) * 2005-04-15 2006-11-02 Hitachi Software Eng Co Ltd Inspection chip and inspection chip system
US20060257907A1 (en) * 2005-04-19 2006-11-16 The Regents Of The University Of California Packed bed for nucleic acid capture and amplification
US20060257958A1 (en) * 2005-05-13 2006-11-16 Pronucleotein Biotechnologies, Llc Magnetically-assisted test strip cartridge and method for using same
DE102005027667A1 (en) * 2005-06-15 2006-12-28 Febit Biotech Gmbh Method for quality control of functionalized surfaces
JP2007010341A (en) * 2005-06-28 2007-01-18 Sumitomo Bakelite Co Ltd Immunity analysis method
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
US8257964B2 (en) 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
EP3029135B1 (en) 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
US9354156B2 (en) * 2007-02-08 2016-05-31 Emd Millipore Corporation Microfluidic particle analysis method, device and system
US9376658B2 (en) 2008-01-03 2016-06-28 Emd Millipore Corporation Cell culture array system for automated assays and methods of operation and manufacture thereof
US9637715B2 (en) 2005-07-07 2017-05-02 Emd Millipore Corporation Cell culture and invasion assay method and system
US9388374B2 (en) 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US20070026419A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US7805081B2 (en) * 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
JP2009505634A (en) * 2005-08-31 2009-02-12 日産化学工業株式会社 Microchip for cell response evaluation
JP4699840B2 (en) * 2005-08-31 2011-06-15 ローム株式会社 Biochip and immunoassay method
US7405281B2 (en) * 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7763423B2 (en) * 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
EP1929269A2 (en) * 2005-09-30 2008-06-11 Caliper Life Sciences, Inc. Microfluidic device for purifying a biological component using magnetic beads
US20080311585A1 (en) * 2005-11-02 2008-12-18 Affymetrix, Inc. System and method for multiplex liquid handling
US8075852B2 (en) 2005-11-02 2011-12-13 Affymetrix, Inc. System and method for bubble removal
US20070099288A1 (en) * 2005-11-02 2007-05-03 Affymetrix, Inc. Microfluidic Methods, Devices, and Systems for Fluid Handling
US8007267B2 (en) * 2005-11-02 2011-08-30 Affymetrix, Inc. System and method for making lab card by embossing
US20080038714A1 (en) * 2005-11-02 2008-02-14 Affymetrix, Inc. Instrument to Pneumatically Control Lab Cards and Method Thereof
EP1957983A4 (en) * 2005-11-21 2010-03-24 Univ Columbia Multiplex digital immuno-sensing using a library of photocleavable mass tags
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
DE102005059536B4 (en) 2005-12-13 2008-08-28 Siemens Ag Method and biochip for studying a biological sample
US7913719B2 (en) 2006-01-30 2011-03-29 Cooligy Inc. Tape-wrapped multilayer tubing and methods for making the same
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
US7888017B2 (en) * 2006-02-02 2011-02-15 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
WO2008030631A2 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US7715001B2 (en) * 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7692783B2 (en) * 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
CA2571904A1 (en) * 2006-02-15 2007-08-15 Fio Corporation System and method of detecting pathogens
ES2432391T3 (en) 2006-03-09 2013-12-03 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
WO2007106579A2 (en) * 2006-03-15 2007-09-20 Micronics, Inc. Integrated nucleic acid assays
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
JP5415253B2 (en) 2006-03-24 2014-02-12 ハンディラブ・インコーポレーテッド Integrated system for processing microfluidic samples and methods of use thereof
US20080050747A1 (en) * 2006-03-30 2008-02-28 Pacific Biosciences Of California, Inc. Articles having localized molecules disposed thereon and methods of producing and using same
TW200810676A (en) 2006-03-30 2008-02-16 Cooligy Inc Multi device cooling
US8975216B2 (en) * 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US7563574B2 (en) 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8492168B2 (en) * 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US9476856B2 (en) * 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
JP2009538123A (en) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー Reagents, methods and libraries for gel-free bead-based sequencing
WO2009111769A2 (en) 2008-03-07 2009-09-11 Advanced Liquid Logic, Inc. Reagent and sample preparation and loading on a fluidic device
JP4940756B2 (en) * 2006-05-22 2012-05-30 ソニー株式会社 Micro channel system
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US20080070792A1 (en) 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
US8137912B2 (en) * 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
EP4170042A1 (en) * 2006-06-14 2023-04-26 Verinata Health, Inc. Methods for the diagnosis of fetal abnormalities
WO2007147110A2 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
KR100824209B1 (en) * 2006-06-22 2008-04-24 부산대학교 산학협력단 Device for passive microfluidic washing using capillary force
JP2008008692A (en) * 2006-06-28 2008-01-17 Jeol Ltd Microchip
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
KR100790880B1 (en) 2006-07-05 2008-01-02 삼성전자주식회사 A microfluidic device comprising a microchannel or microchamber bonded to a hydrophobic porous polymer having a magnetic bead therewihin at the wall thereof and method of using the same
US20080044821A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
AU2007289057C1 (en) * 2006-09-01 2014-01-16 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8273310B2 (en) * 2006-09-05 2012-09-25 Samsung Electronics Co., Ltd. Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8101403B2 (en) * 2006-10-04 2012-01-24 University Of Washington Method and device for rapid parallel microfluidic molecular affinity assays
KR100799267B1 (en) * 2006-10-13 2008-01-29 이화여자대학교 산학협력단 Micro or nanofluidic chip fabricated by using the norland optical adhesive and bioanalysis platform prepared by using the same
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
WO2008073378A2 (en) * 2006-12-11 2008-06-19 Thomas Jefferson University Medical College High throughput dna sequencing method and apparatus
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP4134667A1 (en) 2006-12-14 2023-02-15 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
CA2580589C (en) 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
US7932034B2 (en) * 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US8586385B2 (en) 2006-12-28 2013-11-19 Intel Corporation Method and device for biomolecule preparation and detection using magnetic array
US8409877B2 (en) * 2006-12-29 2013-04-02 Intel Corporation Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
US7993525B2 (en) 2006-12-29 2011-08-09 Intel Corporation Device and method for particle complex handling
US8999636B2 (en) 2007-01-08 2015-04-07 Toxic Report Llc Reaction chamber
US8617903B2 (en) * 2007-01-29 2013-12-31 The Invention Science Fund I, Llc Methods for allergen detection
US20080181816A1 (en) * 2007-01-29 2008-07-31 Searete Llc, A Limited Liability Corporation Systems for allergen detection
US10001496B2 (en) * 2007-01-29 2018-06-19 Gearbox, Llc Systems for allergen detection
US20080180259A1 (en) * 2007-01-29 2008-07-31 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Devices for allergen detection
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
WO2008115626A2 (en) 2007-02-05 2008-09-25 Microchip Biotechnologies, Inc. Microfluidic and nanofluidic devices, systems, and applications
DE112008000363B4 (en) 2007-02-05 2021-12-02 Qiagen Sciences, LLC (n.d.Ges.d. Staates Delaware) Device for detection and its use
BRPI0806831B8 (en) 2007-02-09 2021-07-27 Advanced Liquid Logic Inc droplet actuating methods employing magnetic spheres
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
US20080220537A1 (en) * 2007-03-07 2008-09-11 Pacific Biosciences Of California, Inc. Substrates and methods for selective immobilization of active molecules
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US9068977B2 (en) * 2007-03-09 2015-06-30 The Regents Of The University Of Michigan Non-linear rotation rates of remotely driven particles and uses thereof
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US20080234143A1 (en) * 2007-03-23 2008-09-25 Pacific Biosciences Of California, Inc. Mechanical methods for producing substrates having selectively immobilized molecules
US8360321B2 (en) 2007-04-02 2013-01-29 Fio Corporation System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
JP5414667B2 (en) * 2007-05-08 2014-02-12 エフ．ホフマン−ラ ロシュ アーゲー Method for detecting specific immunoglobulin class G antibody
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
US8597729B2 (en) 2007-06-22 2013-12-03 Fio Corporation Systems and methods for manufacturing quantum dot-doped polymer microbeads
US8551786B2 (en) 2007-07-09 2013-10-08 Fio Corporation Systems and methods for enhancing fluorescent detection of target molecules in a test sample
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
CA2693654C (en) 2007-07-13 2018-02-13 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8016260B2 (en) 2007-07-19 2011-09-13 Formulatrix, Inc. Metering assembly and method of dispensing fluid
PT2557517T (en) 2007-07-23 2023-01-04 Univ Hong Kong Chinese Determining a nucleic acid sequence imbalance
US20100112590A1 (en) 2007-07-23 2010-05-06 The Chinese University Of Hong Kong Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing With Enrichment
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
JP2010534474A (en) * 2007-07-26 2010-11-11 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド Molecular redundant sequencing
TW200912621A (en) 2007-08-07 2009-03-16 Cooligy Inc Method and apparatus for providing a supplemental cooling to server racks
GB2463839B (en) * 2007-08-09 2012-09-26 Agilent Technologies Inc Fluid flow control in a microfluidic device
WO2009026359A2 (en) 2007-08-20 2009-02-26 Platypus Technologies, Llc Improved devices for cell assays
US20090062132A1 (en) * 2007-08-29 2009-03-05 Borner Scott R Alternative nucleic acid sequencing methods
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
JP5227556B2 (en) * 2007-09-06 2013-07-03 株式会社日立製作所 Analysis equipment
EP2200744B1 (en) * 2007-09-14 2020-05-27 Biosensia Patents Limited An analysis system
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
WO2009045344A2 (en) * 2007-09-28 2009-04-09 Pacific Biosciences Of California, Inc. Error-free amplification of dna for clonal sequencing
WO2009046149A1 (en) * 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
US20090085427A1 (en) * 2007-10-01 2009-04-02 The Regents Of The University Of Michigan Electrical power generation from fluid flow
EP2209549A4 (en) 2007-10-12 2014-03-05 Fio Corp Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto
US8707964B2 (en) * 2007-10-31 2014-04-29 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US20090105561A1 (en) * 2007-10-17 2009-04-23 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Medical or veterinary digestive tract utilization systems and methods
US8789536B2 (en) 2007-10-17 2014-07-29 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US8303573B2 (en) 2007-10-17 2012-11-06 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
EP4310194A2 (en) 2007-10-19 2024-01-24 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US8808276B2 (en) * 2007-10-23 2014-08-19 The Invention Science Fund I, Llc Adaptive dispensation in a digestive tract
US20090130746A1 (en) * 2007-10-25 2009-05-21 Canon U.S. Life Sciences, Inc. Microchannel surface coating
US20090163894A1 (en) * 2007-10-31 2009-06-25 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Medical or veterinary digestive tract utilization systems and methods
US8109920B2 (en) * 2007-10-31 2012-02-07 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US8808271B2 (en) * 2007-10-31 2014-08-19 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US8333754B2 (en) * 2007-10-31 2012-12-18 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
EP2212437A4 (en) 2007-11-07 2011-09-28 Univ British Columbia Microfluidic device and method of using same
US8049183B1 (en) * 2007-11-09 2011-11-01 Carnegie Mellon University Apparatuses and methods for control and self-assembly of particles into adaptable monolayers
WO2009081409A2 (en) * 2007-12-26 2009-07-02 Seng Enterprises Ltd. Device for the study of living cells
WO2009067632A1 (en) * 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
US20090137866A1 (en) * 2007-11-28 2009-05-28 Searete Llc, A Limited Liability Corporation Of The State Delaware Medical or veterinary digestive tract utilization systems and methods
US20090155840A1 (en) * 2007-12-17 2009-06-18 Beebe David J Method and device for cell counting
JP5738597B2 (en) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ Systems and methods for nucleic acid sequencing
KR20100100974A (en) 2007-12-23 2010-09-15 어드밴스드 리퀴드 로직, 아이엔씨. Droplet actuator configurations and methods of conducting droplet operations
WO2009086353A1 (en) * 2007-12-26 2009-07-09 Helicos Biosciences Corporation Improved two-primer sequencing for high-throughput expression analysis
US8815576B2 (en) * 2007-12-27 2014-08-26 Lawrence Livermore National Security, Llc. Chip-based sequencing nucleic acids
US8802424B2 (en) 2008-01-10 2014-08-12 Pacific Biosciences Of California, Inc. Methods and systems for analysis of fluorescent reactions with modulated excitation
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
EP2255014A4 (en) 2008-02-12 2011-05-18 Pacific Biosciences California Compositions and methods for use in analytical reactions
US8741815B2 (en) * 2008-02-19 2014-06-03 Intelligent Bio Systems, Inc. Methods and devices for amplification of nucleic acid
WO2009111461A2 (en) * 2008-03-04 2009-09-11 University Of Utah Research Foundation Microfluidic flow cell
US8632243B2 (en) * 2008-03-10 2014-01-21 The Hong Kong Polytechnic University Microfluidic mixing using continuous acceleration/deceleration methodology
US8250877B2 (en) 2008-03-10 2012-08-28 Cooligy Inc. Device and methodology for the removal of heat from an equipment rack by means of heat exchangers mounted to a door
US9297571B1 (en) 2008-03-10 2016-03-29 Liebert Corporation Device and methodology for the removal of heat from an equipment rack by means of heat exchangers mounted to a door
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CA2991818C (en) 2008-03-28 2022-10-11 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8236499B2 (en) * 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
US20090269746A1 (en) * 2008-04-25 2009-10-29 Gil Atzmon Microsequencer-whole genome sequencer
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
TWI460602B (en) * 2008-05-16 2014-11-11 Counsyl Inc Device for universal preconception screening
WO2009150583A1 (en) * 2008-06-10 2009-12-17 Koninklijke Philips Electronics N.V. Diagnostic device
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
WO2009155704A1 (en) 2008-06-25 2009-12-30 Fio Corporation Bio-threat alert system
US8470164B2 (en) 2008-06-25 2013-06-25 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20090325159A1 (en) * 2008-06-30 2009-12-31 Canon U.S. Life Sciences, Inc. System and method to prevent cross-contamination in assays performed in a microfluidic channel
US20110281740A1 (en) * 2008-06-30 2011-11-17 Joseph Beechem Methods for Real Time Single Molecule Sequencing
US20100120034A1 (en) * 2008-07-03 2010-05-13 Life Technologies Corporation Methylation analysis of mate pairs
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
WO2010019969A1 (en) * 2008-08-15 2010-02-18 Cornell University Device for rapid identification of nucleic acids for binding to specific chemical targets
GB0815212D0 (en) 2008-08-20 2008-09-24 Roar Particles Ltd Identification of sample components
CN105759021A (en) 2008-08-29 2016-07-13 Fio公司 Single-use Handheld Diagnostic Test Device And Associated System And Method For Testing Biological And Environmental Test Samples
US8795961B2 (en) * 2008-09-05 2014-08-05 Pacific Biosciences Of California, Inc. Preparations, compositions, and methods for nucleic acid sequencing
WO2010033193A2 (en) 2008-09-16 2010-03-25 Pacific Biosciences Of California, Inc. Substrates and optical systems and methods of use thereof
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8481264B2 (en) * 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
WO2010036287A1 (en) * 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8383369B2 (en) * 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8361716B2 (en) 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
WO2010041174A1 (en) 2008-10-06 2010-04-15 Koninklijke Philips Electronics N.V. Microfluidic device
EP2350652A2 (en) * 2008-10-10 2011-08-03 Cnrs-Dae Cell sorting device
WO2010040227A1 (en) * 2008-10-10 2010-04-15 The Governing Council Of The University Of Toronto Hybrid digital and channel microfluidic devices and methods of use thereof
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8252910B2 (en) 2008-11-19 2012-08-28 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
WO2010059235A2 (en) 2008-11-20 2010-05-27 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
EP2370598B1 (en) * 2008-12-11 2017-02-15 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
EP2373812B1 (en) * 2008-12-19 2016-11-09 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
EP2379748A4 (en) 2008-12-23 2012-08-29 Illumina Inc Multibase delivery for long reads in sequencing by synthesis protocols
EP2784508B1 (en) * 2008-12-25 2017-06-14 Universal Bio Research Co., Ltd. Automated nucleic acid assay system
KR20110111449A (en) 2008-12-31 2011-10-11 인터젠엑스 인크. Instrument with microfluidic chip
RU2578023C2 (en) 2009-01-13 2016-03-20 Эф-Ай-Оу Корпорейшн Portable diagnostic unit and method for using it with electronic device and diagnostic cartridge in instant diagnostic tests
DE102009005925B4 (en) 2009-01-23 2013-04-04 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Apparatus and method for handling biomolecules
US8100293B2 (en) 2009-01-23 2012-01-24 Formulatrix, Inc. Microfluidic dispensing assembly
US8569046B2 (en) * 2009-02-20 2013-10-29 Massachusetts Institute Of Technology Microarray with microchannels
WO2010122990A1 (en) * 2009-04-20 2010-10-28 ユニバーサル・バイオ・リサーチ株式会社 Tube for measuring bio-related substance and quantifying system
US20100270160A1 (en) * 2009-04-22 2010-10-28 Naf Technologies Llc Portable Low Power Charged Particle Analysis Device
US20100330569A1 (en) * 2009-04-23 2010-12-30 Intelligent Bio-Systems, Inc. Hydroxymethyl Linkers For Labeling Nucleotides
AU2010245304B2 (en) 2009-04-27 2015-06-04 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
DE102009040151B4 (en) * 2009-05-26 2013-09-12 Analytik Jena Ag Arrangement for the detection of chemiluminescence on gases
WO2010138960A2 (en) * 2009-05-29 2010-12-02 President And Fellows Of Harvard College Methods and systems for single-molecule rna expression profiling
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
CN102459565A (en) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 Fluidic devices with diaphragm valves
AU2010257118B2 (en) 2009-06-04 2014-08-28 Lockheed Martin Corporation Multiple-sample microfluidic chip for DNA analysis
JP2012529268A (en) 2009-06-05 2012-11-22 インテジェンクス，インコーポレイテッド Use of universal sample preparation system and integrated analysis system
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
US10036063B2 (en) * 2009-07-24 2018-07-31 Illumina, Inc. Method for sequencing a polynucleotide template
CN102712950B (en) * 2009-07-29 2015-05-20 皮罗比特私人有限公司 Method and apparatus for conducting an assay
DE102009035941B8 (en) * 2009-08-03 2017-04-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. diagnostic system
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
WO2011026102A1 (en) 2009-08-31 2011-03-03 Life Technologies Corporation Methods of bead manipulation and forming bead arrays
US20110059864A1 (en) * 2009-09-07 2011-03-10 Caerus Molecular Diagnostics Incorporated Sequence Determination By Use Of Opposing Forces
WO2011038241A1 (en) * 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
KR101107315B1 (en) * 2009-09-29 2012-01-20 한국과학기술연구원 A DNA sequencing method using novel nucleoside triphosphates with a fluorescent 3'-O-blocking group as reversible terminators
WO2011050938A1 (en) 2009-10-26 2011-05-05 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
JP5791621B2 (en) 2009-10-27 2015-10-07 プレジデント アンド フェローズ オブ ハーバード カレッジ Droplet generation technology
EP2496716A1 (en) 2009-11-03 2012-09-12 University Of Virginia Patent Foundation Versatile, visible method for detecting polymeric analytes
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8500979B2 (en) * 2009-12-31 2013-08-06 Intel Corporation Nanogap chemical and biochemical sensors
EP2526427A4 (en) * 2010-01-19 2013-07-24 Harvard College Rapid pathogen diagnostic device and method
US9353342B2 (en) 2010-01-21 2016-05-31 Emd Millipore Corporation Cell culture and gradient migration assay methods and devices
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
EP2529238A4 (en) 2010-01-28 2014-10-08 The Regents Of The University Of Michigan Hanging drop devices, systems and/or methods
KR101851117B1 (en) 2010-01-29 2018-04-23 마이크로닉스 인코포레이티드. Sample-to-answer microfluidic cartridge
US8518643B2 (en) * 2010-02-04 2013-08-27 Pacific Biosciences Of California, Inc. Method to improve single molecule analyses
WO2011106693A2 (en) 2010-02-26 2011-09-01 The Regents Of The University Of Michigan Microscale western blot
US9194838B2 (en) 2010-03-03 2015-11-24 Osaka University Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
CN101817495B (en) * 2010-03-25 2012-03-14 湖南大学 Micro fluid control chip and preparation method and application thereof
AU2011236503B2 (en) 2010-04-07 2014-10-30 Biosensia Patents Limited Flow control device for assays
EP2388337B1 (en) * 2010-04-30 2014-07-02 Nxp B.V. Sensing device and manufacturing method thereof
CA2798123C (en) 2010-05-05 2020-06-23 The Governing Council Of The University Of Toronto Method of processing dried samples using digital microfluidic device
WO2011150226A1 (en) 2010-05-26 2011-12-01 Landers James P Method for detecting nucleic acids based on aggregate formation
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US8318094B1 (en) 2010-06-18 2012-11-27 Pacific Biosciences Of California, Inc. Substrate analysis systems
US8351053B2 (en) * 2010-06-25 2013-01-08 The Board Of Trustees Of The University Of Illinois Apparatus and method for in situ testing of microscale and nanoscale samples
EP2588851B1 (en) 2010-06-30 2016-12-21 Life Technologies Corporation Ion-sensing charge-accumulation circuit and method
CN109449171A (en) 2010-06-30 2019-03-08 生命科技公司 For detecting and measuring the transistor circuit of chemical reaction and compound
JP5952813B2 (en) 2010-06-30 2016-07-13 ライフ テクノロジーズ コーポレーション Method and apparatus for testing ISFET arrays
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
WO2012006222A1 (en) 2010-07-03 2012-01-12 Life Technologies Corporation Chemically sensitive sensor with lightly doped drains
JP2012018039A (en) * 2010-07-07 2012-01-26 Sony Corp Microchannel, and microchip, column, device, and method for nucleic acid hybridization
WO2012021733A2 (en) 2010-08-12 2012-02-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US8715932B2 (en) 2010-08-20 2014-05-06 Intel Corporation Nucleic acid sequencing
WO2012024657A1 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US8465922B2 (en) 2010-08-26 2013-06-18 Pacific Biosciences Of California, Inc. Methods and systems for monitoring reactions
US8846331B2 (en) 2010-08-27 2014-09-30 The Regents Of The University Of Michigan Asynchronous magnetic bead rotation sensing systems and methods
US8444835B2 (en) 2010-09-09 2013-05-21 Intel Corporation Electronic and fluidic interface
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
EP2619564B1 (en) 2010-09-24 2016-03-16 Life Technologies Corporation Matched pair transistor circuits
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
WO2012047889A2 (en) 2010-10-04 2012-04-12 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
MX2013004184A (en) 2010-10-15 2013-07-29 Lockheed Corp Micro fluidic optic design.
BR112013008588B1 (en) * 2010-11-23 2020-05-12 Biomerieux Inc. SAMPLE TEST CARDS
JP2012127696A (en) * 2010-12-13 2012-07-05 Sharp Corp Analyzer and analyzing method
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US10526572B2 (en) 2011-04-01 2020-01-07 EMD Millipore Corporaticn Cell culture and invasion assay method and system
WO2012138973A2 (en) 2011-04-06 2012-10-11 The University Of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
US9816993B2 (en) 2011-04-11 2017-11-14 The Regents Of The University Of Michigan Magnetically induced microspinning for super-detection and super-characterization of biomarkers and live cells
CA3082652A1 (en) 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
DE102012008375A1 (en) 2011-04-27 2012-10-31 Genovoxx Gmbh Methods and components for the detection of nucleic acid chains
WO2012151268A1 (en) 2011-05-02 2012-11-08 University Of Virginia Patent Foundation Method and system for high throughput optical and label free detection of analytes
WO2012151289A2 (en) 2011-05-02 2012-11-08 University Of Virginia Patent Foundation Method and system to detect aggregate formation on a substrate
US20150086981A1 (en) 2011-05-04 2015-03-26 Genovoxx Gmbh Nucleoside-triphosphate conjugate and methods for the use thereof
EP2711079B1 (en) 2011-05-09 2018-12-19 Advanced Liquid Logic, Inc. Microfluidic Feedback Using Impedance Detection
ES2870874T3 (en) 2011-05-18 2021-10-27 Diasorin S P A Systems and methods for detecting the presence of a selected volume of material in a sample processing device
WO2012158988A1 (en) 2011-05-18 2012-11-22 3M Innovative Properties Company Systems and methods for valving on a sample processing device
ES2755078T3 (en) 2011-05-18 2020-04-21 Diasorin S P A Systems and methods for volumetric measurement in a sample processing device
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CA2837486C (en) * 2011-05-27 2020-10-27 Genapsys, Inc. Systems and methods for nucleic acid sequencing
DE202011108189U1 (en) 2011-06-08 2011-12-13 Albert-Ludwigs-Universität Freiburg Device and fluidic module for generating a dilution series
AU2012279420A1 (en) 2011-07-06 2014-01-30 Advanced Liquid Logic Inc Reagent storage on a droplet actuator
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
WO2013016413A2 (en) 2011-07-25 2013-01-31 Advanced Liquid Logic Inc Droplet actuator apparatus and system
US20140234892A1 (en) * 2011-09-23 2014-08-21 Siemens Healthcare Diagnostics Inc. Microfluidic device for separating cells from a fluid
DK2761305T3 (en) 2011-09-30 2017-11-20 Becton Dickinson Co United Reagent Strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
TWI489110B (en) * 2011-11-02 2015-06-21 Wistron Corp Biochip
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
US9523682B2 (en) * 2011-11-16 2016-12-20 Becton, Dickinson And Company Methods and systems for detecting an analyte in a sample
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
EP2785868B1 (en) 2011-12-01 2017-04-12 Genapsys Inc. Systems and methods for high efficiency electronic sequencing and detection
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
ES2870857T3 (en) 2011-12-03 2021-10-27 Emd Millipore Corp Microfluidic system for cell culture
KR101911436B1 (en) * 2011-12-13 2018-10-25 삼성전자주식회사 Extremely high aspect ratio filter for capturing cell
CN103160430A (en) * 2011-12-13 2013-06-19 三星电子株式会社 Cell capturing filter having high aspect ratio
EP4249605A3 (en) 2011-12-22 2023-11-15 President And Fellows Of Harvard College Methods for analyte detection
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
EP2872523B1 (en) 2011-12-30 2018-01-17 Abbott Molecular Inc. Microorganism nucleic acid purification from host samples
EP2802411B1 (en) * 2012-01-13 2017-04-05 Koninklijke Philips N.V. Dna sequencing with reagent recycling on wiregrid
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
RU2658773C2 (en) 2012-02-03 2018-06-22 Бектон, Дикинсон Энд Компани System and method of implementation of automated assays on plurality of biological samples
DK2809799T3 (en) * 2012-02-03 2017-07-17 Pyrobett Pte Ltd Rotatable Nucleic Acid Sequencing Lead Platform
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
WO2013163207A1 (en) 2012-04-24 2013-10-31 Pacific Biosciences Of California, Inc. Identification of 5-methyl-c in nucleic acid templates
US9797817B2 (en) 2012-05-03 2017-10-24 The Regents Of The University Of Michigan Multi-mode separation for target detection
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
EP2856178B1 (en) 2012-05-31 2020-07-15 The University of North Carolina at Chapel Hill Dissolution guided wetting of structured surfaces
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
EP2867645B1 (en) 2012-06-27 2019-06-05 Advanced Liquid Logic, Inc. Techniques and droplet actuator designs for reducing bubble formation
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014027580A1 (en) 2012-08-17 2014-02-20 国立大学法人大阪大学 Sample analysis method
EP2888349B1 (en) 2012-08-24 2022-02-09 Yale University System, device and method for high-throughput multi-plexed detection
DE102012109026A1 (en) * 2012-09-25 2014-03-27 Eads Deutschland Gmbh Detection device and detection method for the automatic determination of biomass
US20150233907A1 (en) * 2012-09-27 2015-08-20 Nanospeed Diagnostics Inc. Microfluidic chip for multi-analyte detection
GB201217390D0 (en) 2012-09-28 2012-11-14 Agplus Diagnostics Ltd Test device and sample carrier
CN110579435B (en) 2012-10-15 2023-09-26 纳诺赛莱克特生物医药股份有限公司 System, apparatus and method for particle sorting
KR102061257B1 (en) 2012-11-19 2019-12-31 앱톤 바이오시스템즈, 인코포레이티드 Digital Analysis Of Molecular Analytes Using Single Molecule Detection
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014100743A2 (en) 2012-12-21 2014-06-26 Micronics, Inc. Low elasticity films for microfluidic use
EP2935908B1 (en) 2012-12-21 2019-08-14 PerkinElmer Health Sciences, Inc. Fluidic circuits and related manufacturing methods
WO2014100725A1 (en) 2012-12-21 2014-06-26 Micronics, Inc. Portable fluorescence detection system and microassay cartridge
JP6282036B2 (en) 2012-12-27 2018-02-21 クオンタムバイオシステムズ株式会社 Method and control apparatus for controlling movement speed of substance
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
BR112015010695B1 (en) 2013-01-11 2023-03-07 Becton, Dickinson And Company MICROFLUID DEVICE AND METHOD FOR PERFORMING A LIQUID SAMPLE TEST, METHOD FOR FORMING A MICROFLUID DEVICE, SYSTEM AND KIT
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US20140222837A1 (en) * 2013-02-01 2014-08-07 Frederick G. Strathmann Automated quality tool for monitoring of samples in a high-throughput assay
EP3862435A1 (en) 2013-02-08 2021-08-11 10X Genomics, Inc. Polynucleotide barcode generation
US9650406B2 (en) * 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
WO2014149778A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensors with consistent sensor surface areas
WO2014150851A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
CN105051525B (en) 2013-03-15 2019-07-26 生命科技公司 Chemical device with thin conducting element
US20140264472A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
DK2970356T3 (en) 2013-03-15 2018-08-27 Illumina Cambridge Ltd Modified nucleosides or nucleotides
CN104096526B (en) * 2013-04-03 2016-03-23 国家纳米科学中心 A kind of (per) fluoropolymer microreactor and application thereof
CA2911303C (en) 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
WO2014182847A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Device for preparation and analysis of nucleic acids
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
WO2014197455A1 (en) * 2013-06-03 2014-12-11 University Of Florida Research Foundation, Incorporated Devices and methods for isolating cells
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
ES2943498T3 (en) 2013-08-05 2023-06-13 Twist Bioscience Corp de novo synthesized libraries
MX2016002210A (en) 2013-08-22 2016-06-06 Apton Biosystems Inc Digital analysis of molecular analytes using electrical methods.
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CA2929929A1 (en) 2013-09-18 2015-03-26 Quantum Biosystems Inc. Biomolecule sequencing devices, systems and methods
US9604214B2 (en) * 2013-10-01 2017-03-28 Owl biomedical, Inc. Cell sorting system using microfabricated components
US9803192B2 (en) 2013-10-04 2017-10-31 Cornell University Programmable and reconfigurable microcolumn affinity chromatography device, system, and methods of use thereof
JP2015077652A (en) 2013-10-16 2015-04-23 クオンタムバイオシステムズ株式会社 Nano-gap electrode and method for manufacturing same
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
US9983110B2 (en) 2013-11-04 2018-05-29 The Regents Of The University Of Michigan Asynchronous magnetic bead rotation (AMBR) microviscometer for analysis of analytes
CN113477149B (en) * 2013-11-06 2023-09-12 贝克顿·迪金森公司 Microfluidic devices and methods of making and using the same
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US10018640B2 (en) 2013-11-13 2018-07-10 Becton, Dickinson And Company Optical imaging system and methods for using the same
CN105873681B (en) 2013-11-18 2019-10-11 尹特根埃克斯有限公司 Cartridge and instrument for sample analysis
WO2015077441A2 (en) 2013-11-20 2015-05-28 University Of Florida Research Foundation, Incorporated Antibody and aptamer ensemble for cell isolation and enrichment
WO2015079446A1 (en) * 2013-11-29 2015-06-04 Technion Research & Development Foundation Limited Metod and device for accelerated surface-based reactions
US10125393B2 (en) 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015109126A1 (en) * 2014-01-15 2015-07-23 Caliper Life Sciences, Inc. Method and system of microfluidic immunoassay using magnetic beads
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
KR20170023979A (en) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. Processes and systems for nucleic acid sequence assembly
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
GB201414451D0 (en) * 2014-08-14 2014-10-01 Oxford Gene Technology Operations Ltd Hybridisation column for nucleic acid enrichment
US10487357B2 (en) * 2014-10-03 2019-11-26 Life Technologies Corporation Methods of nucleic acid analysis using terminator nucleotides
US10544455B2 (en) 2014-10-03 2020-01-28 Life Technologies Corporation Sequencing methods, compositions and systems using terminator nucleotides
US9693723B2 (en) 2014-10-14 2017-07-04 Becton, Dickinson And Company Blood sample management using open cell foam
ES2962033T3 (en) 2014-10-14 2024-03-14 Becton Dickinson Co Blood sample management using open cell foam
EP3552690A1 (en) 2014-10-22 2019-10-16 IntegenX Inc. Systems and methods for sample preparation, processing and analysis
WO2016062788A1 (en) 2014-10-24 2016-04-28 Ait Austrian Institute Of Technology Gmbh Microfluidic chip for biological analysis
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN105624020B (en) * 2014-11-07 2017-11-03 深圳华大基因研究院 For the micro-fluidic chip for the base sequence for detecting DNA fragmentation
CA2966967A1 (en) * 2014-11-07 2016-05-12 Oxy Solutions As Apparatus for dissolving gas into a liquid
US10584366B2 (en) 2014-12-03 2020-03-10 IsoPlexis Corporation Analysis and screening of cell secretion profiles
KR20170097712A (en) 2014-12-18 2017-08-28 라이프 테크놀로지스 코포레이션 Methods and apparatus for measuring analytes using large scale fet arrays
TWI794007B (en) 2014-12-18 2023-02-21 美商生命技術公司 Integrated circuit device, sensor device and integrated circuit
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
EP3244992B1 (en) 2015-01-12 2023-03-08 10X Genomics, Inc. Processes for barcoding nucleic acids
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
JP2018508852A (en) 2015-01-13 2018-03-29 １０エックス ゲノミクス，インコーポレイテッド System and method for visualizing structural variation and phase information
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
MX2017010142A (en) 2015-02-09 2017-12-11 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data.
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
BR112017018054A2 (en) 2015-02-24 2018-07-24 10X Genomics Inc Methods for Covering Targeted Nucleic Acid Sequences
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
AU2016229837B2 (en) 2015-03-10 2018-05-24 Becton, Dickinson And Company Biological fluid micro-sample management device
EP3292395A4 (en) * 2015-04-08 2019-01-02 Board of Regents, The University of Texas System Methods and systems for the detection of analytes
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10677793B2 (en) 2015-04-21 2020-06-09 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
US10590461B2 (en) 2015-04-21 2020-03-17 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
US9868947B2 (en) 2015-05-04 2018-01-16 Washington University Compositions and methods for the construction of a random allelic series
US11180522B2 (en) 2015-05-08 2021-11-23 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
EP3303548A4 (en) 2015-06-05 2019-01-02 Miroculus Inc. Evaporation management in digital microfluidic devices
US10464067B2 (en) 2015-06-05 2019-11-05 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
US10150994B2 (en) * 2015-07-22 2018-12-11 Qiagen Waltham, Inc. Modular flow cells and methods of sequencing
US10578606B2 (en) 2015-09-01 2020-03-03 Becton, Dickinson And Company Depth filtration device for separating specimen phases
GB2557529A (en) 2015-09-18 2018-06-20 Twist Bioscience Corp Oligonucleic acid variant libraries and synthesis thereof
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
US10577643B2 (en) * 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
WO2017066231A1 (en) 2015-10-13 2017-04-20 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
FR3042796B1 (en) 2015-10-21 2022-04-29 Centre Nat Rech Scient MICROFLUIDIC DEVICE FOR CONFINING A SAMPLE
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
CN108350487B (en) 2015-11-19 2022-05-27 加利福尼亚太平洋生物科学股份有限公司 Compounds and systems for improved signal detection
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
WO2017095813A1 (en) 2015-11-30 2017-06-08 Intabio, Inc. Devices and methods for sample characterization
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017185026A1 (en) * 2016-04-22 2017-10-26 Complete Genomics, Inc. Reversibly blocked nucleoside analogues and their use
WO2017189525A1 (en) * 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
WO2017197338A1 (en) * 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN109476695A (en) 2016-06-27 2019-03-15 丹娜法伯癌症研究院 Method for measuring RNA translation rate
CA3027166A1 (en) * 2016-06-30 2018-01-04 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
WO2018005390A1 (en) * 2016-06-30 2018-01-04 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
USD812766S1 (en) * 2016-07-12 2018-03-13 EMULATE, Inc. Microfluidic chip for use with a fluid perfusion module
CN106076445B (en) * 2016-07-18 2018-06-15 天津德祥生物技术有限公司 Micro-fluidic reagent card and its detection method and application
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
CN109996876A (en) 2016-08-22 2019-07-09 特韦斯特生物科学公司 The nucleic acid library of de novo formation
CA3034064A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
CN109923216A (en) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 By the detection combination of biomolecule to the method for the single test using fluorescent in situ sequencing
JP7239465B2 (en) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ Methods for preparing nucleic acid sequence libraries for detection by fluorescence in situ sequencing
USD842493S1 (en) * 2016-09-07 2019-03-05 EMULATE, Inc. Microfluidic chip without pressure features for use with a fluid perfusion module
USD816861S1 (en) * 2016-09-07 2018-05-01 EMULATE, Inc. Transparent microfluidic chip without pressure features for use with a fluid perfusion module
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
US9770717B1 (en) * 2016-11-03 2017-09-26 International Business Machines Corporation Microfluidic chip with bead integration system
US11493508B2 (en) 2016-11-11 2022-11-08 IsoPlexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
EP3545284A4 (en) 2016-11-22 2020-07-01 Isoplexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CA3046680A1 (en) * 2016-12-19 2018-06-28 Quantum-Si Incorporated Loading molecules into sample wells for analysis
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108239661B (en) * 2016-12-23 2020-06-12 中国科学院深圳先进技术研究院 Microfluidic device, system and method for introducing substance into cell
JP2020515815A (en) 2016-12-28 2020-05-28 ミロキュラス インコーポレイテッド Digital microfluidic device and method
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018148700A1 (en) 2017-02-13 2018-08-16 Yale University High-throughput single-cell polyomics
JP2020510417A (en) 2017-02-17 2020-04-09 コニク インコーポレイテッド System for detection
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
KR20190133016A (en) 2017-03-17 2019-11-29 앱톤 바이오시스템즈, 인코포레이티드 Sequencing and High Resolution Imaging
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN107515210B (en) * 2017-06-12 2020-04-14 南京大学 Handheld high-flux matrix electrochemiluminescence chip analysis system
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2019006455A1 (en) 2017-06-30 2019-01-03 Solstice Biologics, Ltd. Chiral phosphoramidite auxiliaries and methods of their use
WO2019023133A1 (en) 2017-07-24 2019-01-31 Miroculus Inc. Digital microfluidics systems and methods with integrated plasma collection device
US11280706B2 (en) * 2017-08-01 2022-03-22 Essenlix Corporation Dilution calibration
CN111587149B (en) 2017-09-01 2022-11-11 米罗库鲁斯公司 Digital microfluidic device and method of use thereof
EP3655158A1 (en) * 2017-09-07 2020-05-27 Sony Corporation Particle capturing chamber, particle capturing chip, particle capturing method, apparatus, and particle analysis system
SG11202002194UA (en) 2017-09-11 2020-04-29 Twist Bioscience Corp Gpcr binding proteins and synthesis thereof
MX2020003113A (en) 2017-09-21 2020-09-07 Genapsys Inc Systems and methods for nucleic acid sequencing.
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
KR20240024357A (en) 2017-10-20 2024-02-23 트위스트 바이오사이언스 코포레이션 Heated nanowells for polynucleotide synthesis
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
US20200392444A1 (en) * 2017-12-21 2020-12-17 Politecnico Di Milano Erythrocytes for drug delivery
KR20200106067A (en) 2018-01-04 2020-09-10 트위스트 바이오사이언스 코포레이션 DNA-based digital information storage
JP7333326B2 (en) 2018-01-29 2023-08-24 インタバイオ， エルエルシー Devices, methods and kits for sample characterization
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019173276A1 (en) * 2018-03-06 2019-09-12 Waters Technologies Corporation A high surface area chromatographic device with low pressure drop
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019204510A1 (en) * 2018-04-17 2019-10-24 Reolab Programmable microchannel systems for analyte detection
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
KR20210015954A (en) 2018-05-31 2021-02-10 인타바이오 인코퍼레이티드 Software for microfluidic systems interfaced with mass spectrometry
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
EP3775834A4 (en) 2018-07-09 2021-03-17 Hewlett-Packard Development Company, L.P. Analyte capturing devices with fluidic ejection devices
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC Methods and systems for sample processing or analysis
EP3853382A4 (en) * 2018-09-19 2022-06-22 Apton Biosystems, Inc. Densely-packed analyte layers and detection methods
CN113195511A (en) 2018-09-28 2021-07-30 生捷科技控股公司 Disulfide-linked reversible terminators
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN109939752A (en) * 2018-12-11 2019-06-28 安博特纳米生物科技有限公司 Micro-channel structure and biological detection platform
WO2020126593A1 (en) * 2018-12-17 2020-06-25 Illumina Cambridge Limited Compositions for use in polyunucleotide sequencing
US11782023B2 (en) 2018-12-19 2023-10-10 Regeneron Pharmaceuticals, Inc. Ce-western applications for antibody development
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
TW202043486A (en) 2019-01-29 2020-12-01 美商伊路米納有限公司 Sequencing kits
TW202045709A (en) 2019-01-29 2020-12-16 美商伊路米納有限公司 Flow cells
WO2020163630A1 (en) * 2019-02-06 2020-08-13 Singular Genomics Systems, Inc. Compositions and methods for nucleic acid sequencing
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113785057A (en) 2019-02-26 2021-12-10 特韦斯特生物科学公司 Variant nucleic acid libraries for antibody optimization
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
CN111744562A (en) * 2019-03-28 2020-10-09 天津大学 Micro-fluidic chip for detecting micro-pollutants
CN114206499A (en) 2019-04-08 2022-03-18 米罗库鲁斯公司 Multi-cartridge digital microfluidic devices and methods of use
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies
EP4041310A1 (en) 2019-10-10 2022-08-17 1859, Inc. Methods and systems for microfluidic screening
US20230039014A1 (en) * 2020-01-22 2023-02-09 Roche Sequencing Solutions, Inc. Microfluidic bead trapping devices and methods for next generation sequencing library preparation
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN116157679A (en) * 2020-09-16 2023-05-23 沃特世科技公司 Compounds for system suitability testing of inert liquid chromatography systems and columns
US20240033310A1 (en) * 2020-11-25 2024-02-01 University Of Maryland, College Park Heterogeneous microparticles, and systems and methods of making and use thereof
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2023278704A1 (en) 2021-06-30 2023-01-05 Dana-Farber Cancer Institute, Inc. Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking
FR3126890B1 (en) * 2021-09-13 2024-03-29 Commissariat Energie Atomique Microfluidic component used for measuring electrical impedance through a biological object
WO2023059702A1 (en) * 2021-10-05 2023-04-13 Mayo Foundation For Medical Education And Research Cell isolation and reagent exchange in a microfluidic device
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation

Family Cites Families (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4390403A (en) 1981-07-24 1983-06-28 Batchelder J Samuel Method and apparatus for dielectrophoretic manipulation of chemical species
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
SE458968B (en) * 1987-06-16 1989-05-22 Wallac Oy BIOSPECIFIC ANALYTICAL PROCEDURE FOR MULTIPLE ANALYTICS WHICH DO NOT INCLUDE PARTICULAR COATING AND LABELING WITH FLUORESCING LABEL SUBSTANCES
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
EP0376611A3 (en) 1988-12-30 1992-07-22 The Board Of Trustees Of The Leland Stanford Junior University Electrophoretic system
US5122614A (en) 1989-04-19 1992-06-16 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5770029A (en) 1996-07-30 1998-06-23 Soane Biosciences Integrated electrophoretic microdevices
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5187085A (en) 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5498392A (en) 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
AU5827294A (en) * 1992-12-11 1994-07-04 Chiron Corporation Synthesis of encoded polymers
FR2703052B1 (en) 1993-03-26 1995-06-02 Pasteur Institut New method of nucleic acid sequencing.
EP0620432B1 (en) 1993-04-15 2004-08-25 Zeptosens AG Method for controlling sample introduction in microcolumn separation techniques and sampling device
DE4316320A1 (en) * 1993-05-15 1994-11-17 Degussa Process for the production of sodium perborate monohydrate
NZ267842A (en) 1993-05-28 1997-09-22 Baylor College Medicine Apparatus for measuring molecular mass (by mass spectrometry) in which the sample is ionised on the sample holder and desorbed therefrom by laser pulses
US5525711A (en) 1994-05-18 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pteridine nucleotide analogs as fluorescent DNA probes
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5650234A (en) 1994-09-09 1997-07-22 Surface Engineering Technologies, Division Of Innerdyne, Inc. Electrophilic polyethylene oxides for the modification of polysaccharides, polypeptides (proteins) and surfaces
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5571410A (en) 1994-10-19 1996-11-05 Hewlett Packard Company Fully integrated miniaturized planar liquid sample handling and analysis device
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US5603351A (en) 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
JP3309644B2 (en) * 1995-05-26 2002-07-29 ソニー株式会社 Data cartridge drive
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5747349A (en) 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
US6399023B1 (en) 1996-04-16 2002-06-04 Caliper Technologies Corp. Analytical system and method
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
GB2313147B (en) 1996-05-14 2000-08-23 Europ Component Co Ltd Seat belt buckle
WO1998000705A1 (en) 1996-06-28 1998-01-08 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
US5779868A (en) 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
NZ333346A (en) * 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
US5699157A (en) 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
US6447727B1 (en) 1996-11-19 2002-09-10 Caliper Technologies Corp. Microfluidic systems
US6136543A (en) * 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US6235471B1 (en) * 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
JP2001521622A (en) * 1997-04-04 2001-11-06 カリパー テクノロジーズ コーポレイション Closed-loop biochemical analyzer
US5976336A (en) 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
AU727083B2 (en) 1997-04-25 2000-11-30 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US6090251A (en) 1997-06-06 2000-07-18 Caliper Technologies, Inc. Microfabricated structures for facilitating fluid introduction into microfluidic devices
US5869004A (en) 1997-06-09 1999-02-09 Caliper Technologies Corp. Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems
US6524790B1 (en) 1997-06-09 2003-02-25 Caliper Technologies Corp. Apparatus and methods for correcting for variable velocity in microfluidic systems
US5882465A (en) 1997-06-18 1999-03-16 Caliper Technologies Corp. Method of manufacturing microfluidic devices
US5959291A (en) 1997-06-27 1999-09-28 Caliper Technologies Corporation Method and apparatus for measuring low power signals
US6001231A (en) 1997-07-15 1999-12-14 Caliper Technologies Corp. Methods and systems for monitoring and controlling fluid flow rates in microfluidic systems
US6042721A (en) * 1997-07-23 2000-03-28 Fabco Industries, Inc. Effluent treatment apparatus
US5876675A (en) 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
EP1009995A4 (en) 1997-09-02 2007-05-02 Caliper Life Sciences Inc Microfluidic system with electrofluidic and electrothermal controls
US5965410A (en) 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6012902A (en) 1997-09-25 2000-01-11 Caliper Technologies Corp. Micropump
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
US5948227A (en) 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6430512B1 (en) 1997-12-30 2002-08-06 Caliper Technologies Corp. Software for the display of chromatographic separation data
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6100541A (en) 1998-02-24 2000-08-08 Caliper Technologies Corporation Microfluidic devices and systems incorporating integrated optical elements
JP3944996B2 (en) 1998-03-05 2007-07-18 株式会社日立製作所 DNA probe array
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
EP1046032A4 (en) * 1998-05-18 2002-05-29 Univ Washington Liquid analysis cartridge
US6306590B1 (en) * 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
EP1104491A4 (en) 1998-08-11 2003-01-29 Caliper Techn Corp Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes
CA2361923A1 (en) * 1999-02-03 2000-08-10 Alexander Sassi Multichannel control in microfluidics
ATE556149T1 (en) * 1999-02-23 2012-05-15 Caliper Life Sciences Inc MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS
US20010035351A1 (en) 2000-03-10 2001-11-01 Simpson Peter C. Cross channel device for serial sample injection

Also Published As

Publication number Publication date
ATE469699T1 (en) 2010-06-15
US20170356040A1 (en) 2017-12-14
WO2000050172A1 (en) 2000-08-31
US6613513B1 (en) 2003-09-02
US20030215862A1 (en) 2003-11-20
DE60045917D1 (en) 2011-06-16
US20040096960A1 (en) 2004-05-20
US7566538B2 (en) 2009-07-28
US20060275817A1 (en) 2006-12-07
EP2177627B1 (en) 2012-05-02
US9670541B2 (en) 2017-06-06
US20160040226A1 (en) 2016-02-11
EP1163052A4 (en) 2002-08-14
US20110118139A1 (en) 2011-05-19
AU3372800A (en) 2000-09-14
EP2177627A1 (en) 2010-04-21
EP1163052B1 (en) 2010-06-02
US10138517B2 (en) 2018-11-27
ATE556149T1 (en) 2012-05-15
US7344865B2 (en) 2008-03-18
EP1163052A1 (en) 2001-12-19
WO2000050642A1 (en) 2000-08-31
US6632655B1 (en) 2003-10-14
EP1163369A1 (en) 2001-12-19
EP1163369B1 (en) 2011-05-04
US20090137413A1 (en) 2009-05-28
US20080131904A1 (en) 2008-06-05
EP1163369A4 (en) 2002-08-28
US9101928B2 (en) 2015-08-11
AU3498900A (en) 2000-09-14
DE60044490D1 (en) 2010-07-15
US7105300B2 (en) 2006-09-12

Similar Documents

Publication Publication Date Title
ATE508200T1 (en) SEQUENCING THROUGH INCORPORATION
Xu et al. Solid-phase reversible immobilization in microfluidic chips for the purification of dye-labeled DNA sequencing fragments
EP1959255A3 (en) Closed-loop biochemical analyzers
DE60003845T2 (en) HYBRIDIZATION TEST FOR BIOMATERIAL IN A BIOCHANNEL
ES2134481T3 (en) METHOD AND APPARATUS FOR PRODUCING MICRO-ASSEMBLIES OF BIOLOGICAL SAMPLES.
DE69527613D1 (en) Microreactor for a modular system
WO2001043871A3 (en) Microarray hybridization chamber
US20120053335A1 (en) Microfluidic chaotic mixing systems and methods
CA2374598A1 (en) The use of microfluidic systems in the detection of target analytes using microsphere arrays
DE60127469D1 (en) POTENTIOMETRIC DNA MICROARRAY, METHOD FOR THE PRODUCTION AND METHOD FOR NUCLEIC ACID ANALYSIS
WO2001051663A3 (en) Integrated systems and methods for diversity generation and screening
WO2003100012A3 (en) Microarrays and method for running hybridization reaction for multiple samples on a single microarray
DE69938742D1 (en) INDIVIDUALLY ADDRESSABLE MICRO ELECTROMAGNETIC UNIT MATRIX CHIP
ATE404285T1 (en) MICROFLUID STRUCTURES
BR9506035A (en) Self-addressing, self-assembling and disposable microelectronic systems for molecular analysis and diagnosis
ITBO940193A0 (en) DEVICE FOR MIXING PAINTS, VARNISHES AND FLUID PRODUCTS IN GENERAL AND PROCEDURE FOR ITS CONTROL.
DE60040091D1 (en) REMOVAL OF DYE-MARKED DIDESOXYTERMINATORS FROM DNA SEQUENCING REACTIONS.
FI953990A (en) A method of rendering a nucleic acid amplification reaction product incapable of further amplification, a diagnostic assay method using this method, and a test kit and container suitable for performing the method or assay.
Geissler et al. Microfluidic patterning of miniaturized DNA arrays on plastic substrates
DK0863912T3 (en) New polymeric carriers for nucleic acid synthesis
ATE389177T1 (en) IMMOBILIZATION OF BINDING SUBSTANCES
WO2002010448A3 (en) Photochemical support disc and method for carrying out chemical and biological assays
ID22170A (en) REACTORS FOR CHEMICAL REACTION IN THREE-PHASE SYSTEMS
AU5495194A (en) Flow through membrane nucleic acid hybridization assay
DE3789138D1 (en) Nucleotide sequences that are selectively expressed in pre-B cells and probes for this purpose.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties