CN109939752A - Micro-channel structure and biological detection platform - Google Patents

Micro-channel structure and biological detection platform Download PDF

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Publication number
CN109939752A
CN109939752A CN201811512833.0A CN201811512833A CN109939752A CN 109939752 A CN109939752 A CN 109939752A CN 201811512833 A CN201811512833 A CN 201811512833A CN 109939752 A CN109939752 A CN 109939752A
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China
Prior art keywords
fluid channel
micro
channel
depth
buffer
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CN201811512833.0A
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Chinese (zh)
Inventor
刘远昭
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Shanghai Weiruibo Biotechnology Co ltd
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Abot Nanobiological Technology Co Ltd
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Priority to CN201811512833.0A priority Critical patent/CN109939752A/en
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Abstract

The present invention provides a kind of micro-channel structure and biological detection platform, which includes overflow plate, and overflow plate offers buffer runner, and buffer runner includes: buffering sap cavity, is flowed into for buffer;Fluid channel, for providing buffer, and the outlet with buffering sap cavity for component to be cleaned;Waste liquid chamber, is connected to fluid channel;Wherein, the depth of waste liquid chamber is greater than the depth of fluid channel.Micro-channel structure and biological detection platform provided by the invention, the depth of waste liquid chamber is greater than the depth of fluid channel, in order to avoid the intracavitary waste reflux of waste liquid is into fluid channel, structure is simple, waste reflux can be prevented by only needing the depth difference of setting fluid channel and waste liquid chamber, guarantee the accuracy of testing result.

Description

Micro-channel structure and biological detection platform
Technical field
The invention belongs to biological monitor technical fields, are to be related to a kind of micro-channel structure and biology more specifically Detection platform.
Background technique
In order to quickly screen illness, the diagnosis cause of disease, the reasons such as forensic identification, need using detecting instrument to blood, saliva, The biological samples such as gastric juice are detected, and there is still a need for trained technicians for most quantitative testing in laboratory environments It uses.Understand the physical condition of oneself in real time in order to facilitate patient, part detecting instrument has been reduced to make at home for patient Portable detector.There is discardable module, i.e. disinfection abandons after a single use, can drop mould in portable detector Block is internal usually to have fluid channel in order to guarantee quantitative detection, and sample to be tested and buffer flow into fluid channel first, then Test module is flowed to, to control flow.But in actual use, the liquid in fluid channel is easy reflux, causes to detect As a result inaccurate.
Summary of the invention
The purpose of the present invention is to provide a kind of micro-channel structures, to solve the liquid in fluid channel existing in the prior art The technical problem of testing result inaccuracy caused by liquid solution flows back.
To achieve the above object, the technical solution adopted by the present invention is that: a kind of micro-channel structure, including overflow plate are provided, The overflow plate offers buffer runner, and the buffer runner includes:
Sap cavity is buffered, is flowed into for buffer;
Fluid channel, for providing buffer, and the outlet with the buffering sap cavity for component to be cleaned;
Waste liquid chamber is connected to the fluid channel;
Wherein, the depth of the waste liquid chamber is greater than the depth of the fluid channel.
Further, the depth of the buffering sap cavity is greater than the depth of the fluid channel.
Further, the first choked flow boss for preventing buffer from flowing back is equipped at the bottom wall of the fluid channel entrance.
Further, the second choked flow boss for preventing waste reflux is equipped at the bottom wall of the fluid channel outlet.
Further, check (non-return) valve, the width of the check (non-return) valve are additionally provided between the first choked flow boss and the fluid channel Degree is less than the width of the fluid channel.
Further, the inlet and exit of the buffering sap cavity are equipped with anti-blocking portion.
Further, the anti-blocking portion includes multiple water conservancy diversion microtrabeculaes being spaced apart from each other.
Further, the bottom wall of the fluid channel offers the reaction mouth that detection zone is flowed into for buffer.
Further, the overflow plate has the circumferential rib for preventing leakage, the ring backwards to the fluid channel side Shape gilled rings is set to the border of the reaction mouth.
The present invention also provides a kind of biological detection platforms, including above-mentioned micro-channel structure, further include and the fluid channel Connection and the test board for being used to detect sample.
The beneficial effect of micro-channel structure provided by the invention is: compared with prior art, micro-channel structure of the present invention Overflow plate on offer buffer runner, buffer runner includes buffering sap cavity, fluid channel and waste liquid chamber, is buffered in sap cavity Liquid rinses the component of the needs such as test board cleaning by fluid channel, and waste liquid back flows back into waste liquid chamber, wherein the depth of waste liquid chamber Degree is greater than the depth of fluid channel, in order to avoid the intracavitary waste reflux of waste liquid is into fluid channel, structure is simple, it is only necessary to fluid channel be arranged Waste reflux can be prevented with the depth difference of waste liquid chamber, guarantees the accuracy of testing result.
Detailed description of the invention
It to describe the technical solutions in the embodiments of the present invention more clearly, below will be to embodiment or description of the prior art Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only of the invention some Embodiment for those of ordinary skill in the art without any creative labor, can also be according to these Attached drawing obtains other attached drawings.
Fig. 1 is the structure chart one of micro-channel structure provided in an embodiment of the present invention;
Fig. 2 is the structure chart two of micro-channel structure provided in an embodiment of the present invention;
Fig. 3 is the partial enlarged view of A in Fig. 1;
Fig. 4 is the partial enlarged view of B in Fig. 1.
Wherein, each appended drawing reference in figure:
3- overflow plate;301- avoid holes;31- buffers sap cavity;311- propulsive liquid mouth;312- water conservancy diversion microtrabeculae;32- fluid channel; 3201- reacts mouth;3202- sample inflow hole;321- the first choked flow boss;322- check (non-return) valve;323- the second choked flow boss;33- Waste liquid chamber;34- circumferential rib.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, tie below Accompanying drawings and embodiments are closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only To explain the present invention, it is not intended to limit the present invention.
It should be noted that it can be directly another when element is referred to as " being fixed on " or " being set to " another element On one element or indirectly on another element.When an element is known as " being connected to " another element, it can To be directly to another element or be indirectly connected on another element.
It is to be appreciated that term " length ", " width ", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientation or positional relationship of the instructions such as "horizontal", "top", "bottom" "inner", "outside" is that orientation based on the figure or position are closed System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or Implicitly include one or more of the features.In the description of the present invention, the meaning of " plurality " is two or more, Unless otherwise specifically defined.
It is existing referring to FIG. 1 to FIG. 4, that micro-channel structure provided by the invention is illustrated.The micro-channel structure includes overflowing Flowing plate 3, buffer runner is offered on overflow plate 3, and buffer runner includes buffering sap cavity 31, fluid channel 32 and waste liquid chamber 33. The top of overflow plate 3 is provided with upper cover plate, and upper cover plate is used to seal the top of buffer runner, becomes closed buffer Runner.It buffers sap cavity 31 to flow into for buffer, fluid channel 32 is used to control the flow of buffer or sample to be tested, waste liquid chamber 33 For storing waste liquid, the outlet of fluid channel 32 and buffering sap cavity 31, the outlet of waste liquid chamber 33 and fluid channel 32.Such as This, after buffer enters buffering sap cavity 31, as the liquid in buffering sap cavity 31 gradually increases, buffer enters fluid channel 32 In, the buffer in fluid channel 32 flows into component to be cleaned, provides buffer for component to be cleaned, and the liquid after rinsing returns Into waste liquid chamber 33.Wherein, the depth of waste liquid chamber 33 is greater than the depth of fluid channel 32, guarantees that the liquid in waste liquid chamber 33 will not return It flow in fluid channel 32.More specifically, overflow plate 3 is horizontally disposed, the top of fluid channel 32 and waste liquid chamber 33 is in same level On face, when the depth of waste liquid chamber 33 is greater than the depth of fluid channel 32, the bottom surface of waste liquid chamber 33 is lower than the bottom surface of fluid channel 32, makes to give up The liquid stored in sap cavity 33 will not flow back.
Micro-channel structure provided by the invention opens up on the overflow plate 3 of micro-channel structure of the present invention compared with prior art There is buffer runner, buffer runner includes buffering sap cavity 31, fluid channel 32 and waste liquid chamber 33, buffers the liquid warp in sap cavity 31 The component that fluid channel 32 rinses the cleaning of the needs such as test board is crossed, waste liquid back flows back into waste liquid chamber 33, wherein the depth of waste liquid chamber 33 Degree is greater than the depth of fluid channel 32, in order to avoid the waste reflux in waste liquid chamber 33 is into fluid channel 32, structure is simple, it is only necessary to be arranged Fluid channel 32 and the depth difference of waste liquid chamber 33 can prevent waste reflux, guarantee the accuracy of testing result.
In one of the embodiments, referring to Fig. 1, a kind of specific implementation as micro-channel structure provided by the invention Mode, the depth of fluid channel 32 are less than the depth of buffering sap cavity 31, and the bottom surface of buffering sap cavity 31 is lower than the bottom surface of fluid channel 32, makes When obtaining buffer full of buffering sap cavity 31, Cai Huixiang fluid channel 32 flows, its role is to: first is that rinsing fluid channel as much as possible 32 side wall is convenient for quantitative detection second is that the flow of buffer can be precisely controlled.
Fig. 1 and Fig. 4 is please referred in one of the embodiments, a kind of tool as micro-channel structure provided by the invention Body embodiment is equipped with the first choked flow boss 321 for preventing buffer from flowing back, the first resistance at the bottom wall of 32 entrance of fluid channel Stream boss 321 can be positive the boss that arc-shaped, ellipse arc or corner are arc, and the first choked flow boss 321 is across miniflow The two sides in road 32, height is greater than the depth of fluid channel 32, so that the top on the top of the first choked flow boss 321 and fluid channel 32 Spacing it is smaller, can not only prevent the liquid reflux in fluid channel 32, moreover it is possible to which accurate control enters the liquid in fluid channel 32 Flow.Optionally, the ratio of 32 whole height of the height of the first choked flow boss 321 and fluid channel is between 5/10 to 9/10.
Fig. 1 and Fig. 3 is please referred in one of the embodiments, a kind of tool as micro-channel structure provided by the invention Body embodiment is equipped with the second choked flow boss 323 for preventing waste reflux, the second choked flow at the bottom wall that fluid channel 32 exports Boss 323 can be positive the boss that arc-shaped, ellipse arc or corner are arc, and the second choked flow boss 323 is also across miniflow The two sides in road 32, height is greater than the depth of fluid channel 32, so that the top on the top of the second choked flow boss 323 and fluid channel 32 Spacing it is smaller, can not only prevent the liquid reflux in waste liquid chamber 33, prevent the sample in fluid channel 32 in the detection process It is accurate to control sample flow into waste liquid chamber 33, moreover it is possible to which that accurate control enters the flow of the liquid in waste liquid chamber 33, i.e., accurately Ground controls flushing dose.Optionally, the ratio of the height of the second choked flow boss 323 and 32 whole height of fluid channel is 5/10 to 9/10 Between.
Fig. 1 and Fig. 3 is please referred in one of the embodiments, a kind of tool as micro-channel structure provided by the invention Body embodiment, is additionally provided with check (non-return) valve 322 between the first choked flow boss 321 and fluid channel 32, the width of check (non-return) valve 322 is less than micro- The width of runner 32, so that the liquid in fluid channel 32 can not flow back.Optionally, the width of check (non-return) valve and 32 width of fluid channel Ratio is between 1/12 to 6/12.Certainly, in other embodiments, overflow plate 3 can also only include the first choked flow boss 321 or Check (non-return) valve 322.
Fig. 1 and Fig. 4 is please referred in one of the embodiments, a kind of tool as micro-channel structure provided by the invention Body embodiment, the inlet and exit for buffering sap cavity 31 are equipped with anti-blocking portion 312.The effect in anti-blocking portion 312 is to increase The homogeneity of liquid flowing has certain guiding role to liquid.More specifically, the width of the entrance and exit of buffering sap cavity 31 Degree is respectively less than the width of central cavity, first is that can guarantee the capacity of buffering sap cavity 31, second is that being more convenient for controlling flow.
Fig. 1, Fig. 3 and Fig. 4 are please referred in one of the embodiments, one as micro-channel structure provided by the invention Kind specific embodiment, anti-blocking portion 312 include multiple water conservancy diversion microtrabeculaes being spaced apart from each other.Water conservancy diversion microtrabeculae can be cylindrical, height Less than or equal to the depth of buffering sap cavity 31.When water conservancy diversion microtrabeculae 312 is cylindrical, height and its diameter of water conservancy diversion microtrabeculae Ratio in 1:1 between 1:5, spacing ratio between the diameter and adjacent guide microtrabeculae of water conservancy diversion microtrabeculae 1:0.5 to 1:1 it Between, the cross-sectional area of water conservancy diversion microtrabeculae and the area ratio of buffering sap cavity 31 are between 3% to 15%.Liquid can be between water conservancy diversion microtrabeculae Gap flow through, change liquid buffering sap cavity 31 in original flow path, increase liquid flowing homogeneity.In other realities It applies in example, it is possible to use the strainer with hole replaces water conservancy diversion microtrabeculae.
Fig. 1 and Fig. 4 is please referred to, as a kind of specific embodiment of micro-channel structure provided by the invention, fluid channel 32 Bottom wall offer for buffer flow into detection zone reaction mouth 3201.After buffer enters buffering sap cavity 31, fluid channel is flowed into 32, test board 5 is flowed by reaction mouth 3201, flows into waste liquid chamber 33 after rinsing detection zone.Optionally, 31 inlet of sap cavity is buffered With propulsive liquid mouth 311 compatible with buffer inlet 201, seal the side wall of the side wall for buffering sap cavity 31 and buffer inlet Connection.Optionally, the quantity of reaction mouth 3201 is multiple, and the length direction along fluid channel 32 is sequentially distributed.Detection zone can be use In the test board of detection sample.
Optionally, the bottom wall of fluid channel 32 is also provided with sample inflow hole 3202, the setting of sample inflow hole 3202 so that When sample flows to sample exit port, enter in fluid channel 32 by sample inflow hole 3202, then the reaction from 32 bottom of fluid channel Enter in test board in mouth 3201.More specifically, sample inflow hole 3202 is set to the inlet of fluid channel 32.
Optionally, avoid holes 301 are also provided on overflow plate 3, avoid holes 301 and the entrance of sample face, and sample is worn It crosses avoid holes to flow into the channel that other circulate for sample, for example, flowing into the filter plate for filtering sample, avoid holes 301 Effect be convenient for layout overflow plate 3 and filter plate etc..
In one of the embodiments, referring to Fig. 2, a kind of specific implementation as micro-channel structure provided by the invention Mode, overflow plate 3 have the circumferential rib 34 for preventing leakage backwards to 31 side of fluid channel, and circumferential rib 34 is located on reaction mouth 3201 border.Circumferential rib 34 is abutted against with test board, and the effect of circumferential rib 34 is: abutted against with test board, make liquid from The channel sealing that mouth 3201 enters test board is reacted, prevents buffer from leaking, in assembly, does not need to try using the chemistry such as glue Agent melts connection overflow plate 3 and test board, convenient disassembly.Specifically, the border of each reaction mouth 3201 is provided with a ring Shape rib 34, alternatively, being provided with a circumferential rib 34 on overflow plate 3, which is centered around around all reaction mouths 3201. Optionally, the ratio of the height of circumferential rib 34 and width is in 1:1 between 1:5.The shape of circumferential rib 34 is not construed as limiting herein, energy It is enough to surround reaction mouth 3201.
The present invention also provides a kind of biological detection platforms including the micro-channel structure in any of the above-described embodiment further includes For detecting the test board of sample, test board is connected to fluid channel 32.Specifically, the reaction mouth 3201 and test board of fluid channel 32 It faces, liquid is flowed into test board by reaction mouth 3201.The biological detection platform further include be covered on it is upper on overflow plate 3 Cover board, upper cover plate seal the top of buffer runner.
Biological detection platform provided by the invention offers buffer runner on the overflow plate 3 of micro-channel structure, buffers Liquid stream road includes buffering sap cavity 31, fluid channel 32 and waste liquid chamber 33, and the liquid buffered in sap cavity 31 is surveyed by the flushing of fluid channel 32 The component of the needs such as test plate (panel) cleaning, waste liquid back flow back into waste liquid chamber 33, and wherein the depth of waste liquid chamber 33 is greater than fluid channel 32 Depth, in order to avoid the waste reflux in waste liquid chamber 33 is into fluid channel 32, structure is simple, it is only necessary to fluid channel 32 and waste liquid chamber be arranged 33 depth difference can prevent waste reflux, guarantee the accuracy of testing result.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.

Claims (10)

1. micro-channel structure, which is characterized in that including overflow plate, the overflow plate offers buffer runner, the buffer Runner includes:
Sap cavity is buffered, is flowed into for buffer;
Fluid channel, for providing buffer, and the outlet with the buffering sap cavity for component to be cleaned;
Waste liquid chamber is connected to the fluid channel;
Wherein, the depth of the waste liquid chamber is greater than the depth of the fluid channel.
2. micro-channel structure as described in claim 1, which is characterized in that the depth of the buffering sap cavity is greater than the fluid channel Depth.
3. micro-channel structure as described in claim 1, which is characterized in that be equipped at the bottom wall of the fluid channel entrance for preventing Only the first choked flow boss of buffer reflux.
4. micro-channel structure as described in claim 1, which is characterized in that be equipped at the bottom wall of the fluid channel outlet for preventing Only the second choked flow boss of waste reflux.
5. micro-channel structure as claimed in claim 3, which is characterized in that between the first choked flow boss and the fluid channel It is additionally provided with check (non-return) valve, the width of the check (non-return) valve is less than the width of the fluid channel.
6. micro-channel structure as described in claim 1, which is characterized in that the inlet and exit of the buffering sap cavity are all provided with There is anti-blocking portion.
7. micro-channel structure as claimed in claim 6, which is characterized in that the anti-blocking portion includes multiple water conservancy diversion being spaced apart from each other Microtrabeculae.
8. micro-channel structure as described in claim 1, which is characterized in that the bottom wall of the fluid channel is offered for buffering liquid stream Enter the reaction mouth of detection zone.
9. micro-channel structure as claimed in claim 8, which is characterized in that the overflow plate has backwards to the fluid channel side For preventing the circumferential rib of leakage, the circumferential rib is located on the border of the reaction mouth.
10. biological detection platform, which is characterized in that including the described in any item micro-channel structures of claim 1-9, further include with The fluid channel connection and the test board for detecting sample.
CN201811512833.0A 2018-12-11 2018-12-11 Micro-channel structure and biological detection platform Pending CN109939752A (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050172A1 (en) * 1999-02-23 2000-08-31 Caliper Technologies Corp. Manipulation of microparticles in microfluidic systems
CN103954605A (en) * 2014-04-30 2014-07-30 中国科学院物理研究所 Micro-flow detector based on SERS (Surface Enhanced Raman Scattering) principle and preparation method thereof
CN105879936A (en) * 2016-03-31 2016-08-24 苏州市博纳泰科生物技术有限公司 Whole blood filtration and quantitative transfer micro-fluidic chip
CN106955750A (en) * 2017-04-01 2017-07-18 苏州岚轩生物科技有限公司 The micro-fluidic chip flowed based on active control liquid
CN207703876U (en) * 2018-01-16 2018-08-07 北京旌准医疗科技有限公司 It is a kind of based on micro-fluidic fluorescence immunoassay detection chip
CN108663531A (en) * 2018-05-16 2018-10-16 清华大学 Sample amounts unit and micro-fluidic chip with it
CN108686721A (en) * 2017-04-06 2018-10-23 美康生物科技股份有限公司 Micro-fluidic chip and its detection method for whole blood sample separation detection
CN209646519U (en) * 2018-12-11 2019-11-19 上海微瑞博生物科技有限公司 Micro-channel structure and biological detection platform

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050172A1 (en) * 1999-02-23 2000-08-31 Caliper Technologies Corp. Manipulation of microparticles in microfluidic systems
CN103954605A (en) * 2014-04-30 2014-07-30 中国科学院物理研究所 Micro-flow detector based on SERS (Surface Enhanced Raman Scattering) principle and preparation method thereof
CN105879936A (en) * 2016-03-31 2016-08-24 苏州市博纳泰科生物技术有限公司 Whole blood filtration and quantitative transfer micro-fluidic chip
CN106955750A (en) * 2017-04-01 2017-07-18 苏州岚轩生物科技有限公司 The micro-fluidic chip flowed based on active control liquid
CN108686721A (en) * 2017-04-06 2018-10-23 美康生物科技股份有限公司 Micro-fluidic chip and its detection method for whole blood sample separation detection
CN207703876U (en) * 2018-01-16 2018-08-07 北京旌准医疗科技有限公司 It is a kind of based on micro-fluidic fluorescence immunoassay detection chip
CN108663531A (en) * 2018-05-16 2018-10-16 清华大学 Sample amounts unit and micro-fluidic chip with it
CN209646519U (en) * 2018-12-11 2019-11-19 上海微瑞博生物科技有限公司 Micro-channel structure and biological detection platform

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