CN111744562A

CN111744562A - Micro-fluidic chip for detecting micro-pollutants

Info

Publication number: CN111744562A
Application number: CN201910241438.1A
Authority: CN
Inventors: 邹强; 雷志明
Original assignee: Tianjin University
Current assignee: Tianjin University
Priority date: 2019-03-28
Filing date: 2019-03-28
Publication date: 2020-10-09

Abstract

The invention relates to the technical field of single-chip microcomputers, in particular to a micro-fluidic chip for detecting micro-pollutants, which is characterized in that: comprises a first acrylic plate and a second acrylic plate; nine engraved grooves are formed in the first acrylic plate and used for storing detection liquid and providing detection spaces, the first to seventh grooves are used for storing detection objects and objects to be detected, the eighth groove is used for storing waste liquid, and the ninth groove is used for carrying out reaction. The device is small and portable, simple in structure, low in cost, strong in stability, long in service cycle and free of interference to detection.

Description

Micro-fluidic chip for detecting micro-pollutants

Technical Field

The invention relates to the technical field of single-chip microcomputers, in particular to a micro-fluidic chip for detecting micro-pollutants.

Background

Along with the development of industry in recent years, the phenomenon of environmental pollution caused by human activities is more and more serious. Heavy metal in coal pollutes an atmospheric system after being combusted, untreated industrial wastewater pollutes rivers after being illegally discharged, paint on the surfaces of ships peels off and falls into the sea due to time accumulation, and a large amount of heavy metal pollutants flow into the sea due to leakage of a large amount of petroleum and the like caused by accidents of some cargo ships during driving and breakage of conveying pipelines of offshore oil drilling platforms, so that serious pollution is caused. These pollutants enter the body of living things and are accumulated, and finally enter the human body by taking food as a medium, thus seriously harming the human health. Therefore, environmental pollution detection is very important.

The existing environmental pollution detection equipment has a plurality of inconveniences: the devices are large in size and cannot be carried about, and manual work is needed to extract samples from specific positions during detection. Secondly, the detection time and frequency are artificially limited, and real-time detection cannot be realized. And the detection process is complicated and time-consuming.

Disclosure of Invention

The present invention is directed to overcoming the above-mentioned deficiencies, and to providing a microfluidic chip for detecting micro-contaminants.

In order to achieve the purpose, the invention adopts the following technical scheme:

a microfluidic chip for detecting micropollutants, comprising: comprises a first acrylic plate and a second acrylic plate; nine engraved grooves are formed in the first acrylic plate and used for storing detection liquid and providing detection spaces, wherein the first to seventh grooves are used for storing detection objects and objects to be detected, the eighth groove is used for storing waste liquid, and the ninth groove is used for carrying out reaction; the second ya keli board lid is on first ya keli board, closely laminate two boards with the super glue, corresponding position can punch on the board, be connected with the hose through the sucking disc, and connect to on the air pump, the aperture is 1.5mm, the drive division is by 9 air pumps, wherein first air pump ~ seventh air pump is for blowing the air pump, the eighth air pump is for blowing/aspirator pump, switch operating condition as required, can blow into a specified inslot with a certain amount of liquid during blowing, it alright stir to continuously blow, and mainly used inhales the waste liquid into eighth groove when breathing in, by the work of each air pump of singlechip control, with blow, the mode of breathing in realizes mixing, the stirring, the row is useless.

Preferably, the first groove to the seventh groove are 2mm deep, 35mm long and 3mm wide; the depth of the eighth groove and the tenth groove is 2mm, the length of the eighth groove and the tenth groove is 40mm, and the width of the eighth groove and the tenth groove is 30 mm; the ninth groove is 2mm deep, 30mm long, 30mm wide, 1mm channel wide, 2mm deep.

Compared with the prior art, the device has the advantages of small size, portability, simple structure, low cost, strong stability, long service cycle and no interference to detection.

Drawings

FIG. 1 is a schematic structural diagram of a first acrylic plate;

FIG. 2 is a schematic structural view of a second acrylic plate;

fig. 3 is a schematic plan view of the microfluidic chip.

Detailed Description

The following detailed description of the preferred embodiments will be made with reference to the accompanying drawings. As shown in fig. 1-2, a microfluidic chip for detecting micro-pollutants, which is formed by splicing two acrylic plates to form a sealing groove for storing liquid and mixing reaction, wherein the acrylic plates have the advantages of good weather resistance, acid and alkali resistance, long service life, strong impact resistance and the like. The groove 1 is DNA, the groove 2 is 30nm gold nanoparticles, the groove 3 is 2-naphthylthiol, the groove 4 is tris (2-carboxyethyl) phosphine hydrochloride (TCEP), the groove 5 is Phosphate Buffer Solution (PBS), the groove 6 is sodium chloride (NaCl), and the groove 7 is an object to be detected (such as mercury ions).

As shown in FIG. 3, first, the gold nanoparticles in the bath 2 are blown into the reaction bath by the air pump 2, then the 2-naphthylthiol in the bath 3 is blown into the reaction bath 9 by the air pump 3, and then the air blowing operation is continued by the air pump 8 for homogenizing the two substances to be sufficiently mixed, and the blown air is discharged from the hole 9. Then, the phosphate buffer solution in the tank 5 was blown into the reaction tank by the air pump 5 to maintain the pH of the solution at neutral. Then, the reaction mixture was allowed to stand still for five minutes, and then the DNAs in the tanks 1 and 4 and tris (2-carboxyethyl) phosphine hydrochloride were blown into the reaction tank simultaneously with the air pumps 1 and 4, followed by stirring and thorough mixing by an air blowing operation. The TCPE has the function of activating DNA strands so that the DNA strands can be combined on the surface of the gold nanoparticles. The reaction mixture was left to stand for reaction, and after five minutes, sodium chloride in the tank 6 was blown into the reaction tank. And then, continuously blowing the solution by using an air pump 8, fully stirring the solution, and standing the solution for four hours to age the DNA chains so as to erect the DNA chains on the surfaces of the gold nanoparticles. Then, the mercury ion, which is the substance to be measured in the tank 7, is added into the solution for reaction in the last step. One end of a DNA chain is provided with a sulfydryl, the sulfydryl can be fixed on the surface of the gold nanoparticle and combined with gold to form a gold sulfydryl bond, DNA can be absorbed together after mercury ions are added, meanwhile, the gold nanoparticle can also be absorbed together to generate aggregation, 2-naphthylthiol on the gold nanoparticle can generate a Raman signal after the aggregation, SERS detection is carried out after half an hour, a laser beam is aligned to a reaction tank to obtain a Raman spectrogram of the 2-naphthylthiol, and the mercury ions can be detected.

The whole microfluidic chip is vertically arranged, the liquid in the grooves can not flow randomly due to the convolution design of the grooves, the two acrylic plates are tightly attached, and series flow cannot be formed between the grooves.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A microfluidic chip for detecting micropollutants, comprising: comprises a first acrylic plate and a second acrylic plate; nine engraved grooves are formed in the first acrylic plate and used for storing detection liquid and providing detection spaces, wherein the first to seventh grooves are used for storing detection objects and objects to be detected, the eighth groove is used for storing waste liquid, and the ninth groove is used for carrying out reaction; the second ya keli board lid is on first ya keli board, closely laminate two boards with the super glue, corresponding position can punch on the board, be connected with the hose through the sucking disc, and connect to on the air pump, the aperture is 1.5mm, the drive division is by 9 air pumps, wherein first air pump ~ seventh air pump is for blowing the air pump, the eighth air pump is for blowing/aspirator pump, switch operating condition as required, can blow into a specified inslot with a certain amount of liquid during blowing, it alright stir to continuously blow, and mainly used inhales the waste liquid into eighth groove when breathing in, by the work of each air pump of singlechip control, with blow, the mode of breathing in realizes mixing, the stirring, the row is useless.

2. A microfluidic chip for the detection of micropollutants as claimed in claim 1, wherein: the first groove to the seventh groove are 2mm deep, 35mm long and 3mm wide; the depth of the eighth groove and the tenth groove is 2mm, the length of the eighth groove and the tenth groove is 40mm, and the width of the eighth groove and the tenth groove is 30 mm; the ninth groove is 2mm deep, 30mm long, 30mm wide, 1mm channel wide, 2mm deep.