CN1715916A - Micro-nano biochemical sensing chip for biochemical molecule detection - Google Patents
Micro-nano biochemical sensing chip for biochemical molecule detection Download PDFInfo
- Publication number
- CN1715916A CN1715916A CN 200510012233 CN200510012233A CN1715916A CN 1715916 A CN1715916 A CN 1715916A CN 200510012233 CN200510012233 CN 200510012233 CN 200510012233 A CN200510012233 A CN 200510012233A CN 1715916 A CN1715916 A CN 1715916A
- Authority
- CN
- China
- Prior art keywords
- micro
- nano
- biochemical
- micromechanics
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 229910052751 metal Inorganic materials 0.000 claims abstract description 24
- 239000002184 metal Substances 0.000 claims abstract description 24
- 239000002086 nanomaterial Substances 0.000 claims abstract description 15
- 239000002699 waste material Substances 0.000 claims abstract description 7
- 239000012530 fluid Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 8
- 239000011159 matrix material Substances 0.000 claims description 6
- 239000002096 quantum dot Substances 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 2
- 241000700605 Viruses Species 0.000 abstract description 2
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 230000005540 biological transmission Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 238000002560 therapeutic procedure Methods 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000005452 bending Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000003795 desorption Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001459 lithography Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000000985 reflectance spectrum Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002341 toxic gas Substances 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A micro-nano biochemical sensing chip for biochemical molecule detection is characterized in that: a micro-mechanical valveless pump and a micro-nano biochemical sensor are integrated together to form a micro-nano biochemical sensing chip, and the micro-mechanical valveless pump mainly comprises a liquid inlet, a liquid storage tank, a micro-channel, a metal micro-nano structure sensor, a left differential resistance flow channel, a right differential resistance flow channel, a micro-mechanical valveless pump, a waste liquid tank and a liquid outlet. The invention has the advantages of compact structure, small volume, high efficiency and the like, and can be used in the fields of DNA molecule and protein calibration and identification, drug transmission, molecular devices, virus detection, bacteria detection, micro-tissue diagnosis, active therapy and the like.
Description
Technical field
The present invention relates to a kind of micro nano biochemical sensitive chip that biochemical molecular detects that is used for.
Background technology
Along with improving constantly of living standards of the people, people have higher requirement to clinical diagnosis and the monitoring and the processing of biochemical compositions such as biomedicine, judicial expertise, food hygiene product supervision analysis, drug development, industry and mining field toxic gas.Traditional biochemical molecular is surveyed identification and mainly is to use radioactive isotope, enzyme or fluorescence etc. as marker, there is the low shortcoming of security and poor stability, efficient and sensitivity, can not satisfy the fast demand of (so that handling and control danger in real time), sensitive (so that surveying extremely toxic substance of trace), special efficacy (getting rid of the interference and the pollution of non-pathogenic composition), urgent wish development high sensitivity of new generation, efficiently, accurately, easily and fast, economic biochemical molecular detection technique.
Summary of the invention
The technical issues that need to address of the present invention are: overcome the deficiency of above-mentioned technology, provide a kind of efficient, high sensitivity, accurately, easily and fast, economical be used for the micro nano biochemical sensitive chip that biochemical molecular detects.
Technical solution of the present invention is: a kind of micro nano biochemical sensitive chip that is used for the biochemical molecular detection, it is characterized in that: micromechanics valveless pump and micro nano biochemical sensitive device are integrated together the formation micro nano biochemical sensitive chip, mainly comprise inlet, liquid storage tank, fluid channel, the metal micro-nano structure sensor, different choked flow road, the left and right sides, the micromechanics valveless pump, waste liquid pool, liquid outlet, biomolecule liquid flows into liquid storage tank by inlet, enter by the metal Nano structure sensor through fluid channel, the part biological molecule can be attached to the surface of metal micro-nanostructure sensor, enter in the micromechanics valveless pump through different choked flow road, a left side again, different choked flow road pumps into waste liquid pool to unnecessary biomolecule liquid through the right side when the pump chamber generating period of micromechanics valveless pump changes, after liquid outlet is discharged biochemical sensitive chip.
The metal micro-nano structure sensor is a cycle metal nano dot matrix, adopts micro-nano process equipment (as the beamwriter lithography machine) to make, and the minimum feature size of cycle metal nano dot matrix is below 100 nanometers, and the cycle is hundred nanometer scale.
Different choked flow road can adopt the Tesla runner of Nicola Tesla invention.The principle of work in different choked flow road is: when forward flow, fluid flows through the sprue that width is big, bending angle is less, total length is less, and its flow resistance is very little; When reversed flow, the direction that fluid will at first be selected owing to inertia and original momentum orientation angle is less is advanced, so quite a few fluid will enter the less side runner of width.The length of side runner is greater than sprue, and bending angle is also greater than sprue, and sectional area is less than sprue.The Way out of side runner will be designed to and the angle of sprue working direction formation greater than 90 °, thereby constitute the resistance that the sprue fluid is advanced.
Principle of the present invention is: different choked flow road relies on its different flow resistance to play movement-oriented effect, when the pump chamber of micromechanics valveless pump sucks liquid, liquid in the different choked flow road, a left side flows along positive dirction, liquid in the right different choked flow road flows along negative direction, because the directional selectivity in different choked flow road, the fluid flow that flows into pump chamber from the left side is greater than the flow that flows into from the right side.When the pump chamber discharged liquid, the liquid in the left different choked flow road flows along negative direction, and the liquid in the right different choked flow road flows along positive dirction, and in like manner, the fluid flow that flows out pump chamber from the right side is greater than the flow that flows out from the left side.Through such work period, with a part of flow pump in left side to the right side.The light that light source sends is used to excite the non-radiative surface plasma in the metal film, macromolecular absorption and desorption have changed local indexes of refraction on the sensor surface, the wave vector of surface plasma changes, when light under the condition of the wave vector coupling of the wave vector component on sensing unit surface and surface plasma, resonance causes the energy loss in reflected light or the transmitted light.
The present invention has following advantage than prior art:
(1) micromechanics valveless pump and micro nano biochemical sensitive device are integrated together the formation micro nano biochemical sensitive chip, have compact conformation, little, the efficient advantages of higher of volume, can be used for the control of express-analysis and level process.
(2) the reciprocal valveless pump of micromechanics is simple in structure, and can be designed to plane figure, is particularly suitable for adopting fine process to make.
(3) the surface plasma sensing unit among the present invention can be used to recycling.In contrast, traditional immunoassays comprise the immunosorbent chemical examination that enzyme interrelates, and can only use once basically.
(4) traditional biochemical molecular detection identification mainly is to use radioactive isotope, enzyme or fluorescence etc. as marker, has the low shortcoming of security and poor stability, efficient and sensitivity.The present invention utilizes the good electric magnetic property and the bioaffinity of metal micro-nanostructure that biochemical molecular is detected, and can satisfy the demand of (so that handling and control danger in real time), sensitive (so that surveying the extremely toxic substance of trace), special efficacy (getting rid of the interference and the pollution of non-pathogenic composition) fast.
The present invention can realize new biochemical sensitive Detection Techniques, for establishing new technical foundation in fields such as dna molecular, protein demarcation and identification, drug delivery, molecular device, virus detection, bacterium detection, microstructure diagnosis and active treatment.
Description of drawings
Fig. 1 is a structured flowchart of the present invention;
Fig. 2 is the fundamental diagram of micromechanics valveless pump of the present invention;
Fig. 3 is the structural drawing of metal micro-nanostructure sensor of the present invention;
Fig. 4 is a course of work synoptic diagram of the present invention.
Embodiment
As shown in Figure 1, the present invention is by inlet 1, liquid storage tank 2, fluid channel 3, metal micro-nano sensor 4, different choked flow road, the left and right sides 7, micromechanics valveless pump 8, waste liquid pool 9, liquid outlet 10 is formed, biomolecule liquid flows into liquid storage tank 2 by inlet 1, enter the metal micro-nano sensor 4 that constitutes by metal micro-nanostructure 5 through fluid channel 3, part biological molecule 6 can be attached to the surface of metal micro-nanostructure 5, when the pump chamber generating period of micromechanics valveless pump 8 changes, as shown in Figure 2, different choked flow road 7 flows into waste liquid pool 9 to unnecessary biomolecule liquid through the left and right sides, after liquid outlet 10 is discharged biochemical sensitive chip.Wherein micromechanics valveless pump 8 can be designed to plane figure, and this structure is the simplest, be convenient to processing, is particularly suitable for adopting fine process to make.
As shown in Figure 3, the metal micro-nanostructure sensor is a cycle metal nano dot matrix, adopts micro-nano process equipment (as the beamwriter lithography machine) to make on metal (as silver) film, and the minimum feature size of dot matrix is below 100 nanometers, cycle is the 100-900 nanometer, i.e. hundred nanometer scale.
As shown in Figure 4, the light that light source sends has on the metal micro-nanostructure sensor of biomolecule and produces surface plasma wave through shining surface attachment after the collimated, when light under the condition of the wave vector coupling of the wave vector component on sensing unit surface and surface plasma, resonance causes the energy loss of reflected light or transmitted light, and this is a tangible low ebb in reflectance spectrum or transmitted spectrum.Reflectance spectrum or transmitted spectrum are converted to electric signal by CCD with light signal and send into computer processing system analysis through being imaged on behind the spectrometer on the CCD.
If light source is a white light, by the detectable biomolecule of the variation of absorption spectrum.If light source is a monochromatic light, the resonance angle depends on the refractive index profile of the interior sample of evanescent wave scope on the sensor surface consumingly.Macromolecular absorption and desorption have changed local indexes of refraction on the sensor surface, make the resonance angle take place one and move, and this moves relevant with the kind of biochemical molecular.
Claims (3)
1, a kind of micro nano biochemical sensitive chip that is used for the biochemical molecular detection, it is characterized in that: micromechanics valveless pump and micro nano biochemical sensitive device are integrated together the formation micro nano biochemical sensitive chip, mainly comprise inlet, liquid storage tank, fluid channel, the metal micro-nano structure sensor, different choked flow road, the left and right sides, the micromechanics valveless pump, waste liquid pool, liquid outlet, biomolecule liquid flows into liquid storage tank by inlet, enter by the metal Nano structure sensor through fluid channel, the part biological molecule can be attached to the surface of metal micro-nanostructure sensor, enter in the micromechanics valveless pump through different choked flow road, a left side again, different choked flow road pumps into waste liquid pool to unnecessary biomolecule liquid through the right side when the pump chamber generating period of micromechanics valveless pump changes, after liquid outlet is discharged biochemical sensitive chip.
2, the micro nano biochemical sensitive chip of realization biochemical molecular detection according to claim 1, it is characterized in that: the cycle metal nano dot matrix of described metal micro-nano structure sensor for adopting the micro-nano process equipment to make, the minimum feature size of cycle metal nano dot matrix is below 100 nanometers, and the cycle is hundred nanometer scale.
3, the micro nano biochemical sensitive chip of realization biochemical molecular detection according to claim 1, it is characterized in that: described micromechanics valveless pump can be designed to plane figure, is particularly suitable for adopting fine process to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510012233 CN1715916B (en) | 2005-07-21 | 2005-07-21 | Micro-nano biochemical sensing chip for biochemical molecule detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510012233 CN1715916B (en) | 2005-07-21 | 2005-07-21 | Micro-nano biochemical sensing chip for biochemical molecule detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1715916A true CN1715916A (en) | 2006-01-04 |
| CN1715916B CN1715916B (en) | 2011-04-20 |
Family
ID=35821927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510012233 Expired - Fee Related CN1715916B (en) | 2005-07-21 | 2005-07-21 | Micro-nano biochemical sensing chip for biochemical molecule detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1715916B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103105487A (en) * | 2011-11-10 | 2013-05-15 | 五鼎生物技术股份有限公司 | Reaction cassette and assay device |
| CN104049029A (en) * | 2014-07-02 | 2014-09-17 | 重庆大学 | Multi-channel magneto-elastic sensor detection chip |
| CN104502303A (en) * | 2015-01-20 | 2015-04-08 | 中国人民解放军第三军医大学第一附属医院 | Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof |
| CN107941706A (en) * | 2012-12-17 | 2018-04-20 | 伦珂德克斯有限公司 | system and method for measuring chemical state |
-
2005
- 2005-07-21 CN CN 200510012233 patent/CN1715916B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103105487A (en) * | 2011-11-10 | 2013-05-15 | 五鼎生物技术股份有限公司 | Reaction cassette and assay device |
| CN107941706A (en) * | 2012-12-17 | 2018-04-20 | 伦珂德克斯有限公司 | system and method for measuring chemical state |
| CN104049029A (en) * | 2014-07-02 | 2014-09-17 | 重庆大学 | Multi-channel magneto-elastic sensor detection chip |
| CN104049029B (en) * | 2014-07-02 | 2017-05-10 | 重庆大学 | Multi-channel magneto-elastic sensor detection chip |
| CN104502303A (en) * | 2015-01-20 | 2015-04-08 | 中国人民解放军第三军医大学第一附属医院 | Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof |
| CN104502303B (en) * | 2015-01-20 | 2017-05-31 | 中国人民解放军第三军医大学第一附属医院 | For the Asia-Pacific hertz nano biological sensor and its detection method of fast frame inspection bacterium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1715916B (en) | 2011-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11268887B2 (en) | Manipulation of microfluidic droplets | |
| Shim | Diffusiophoresis, diffusioosmosis, and microfluidics: surface-flow-driven phenomena in the presence of flow | |
| Gong et al. | New advances in microfluidic flow cytometry | |
| McClain et al. | Flow cytometry of Escherichia coli on microfluidic devices | |
| Lee et al. | Nanoparticle size detection limits by single particle ICP-MS for 40 elements | |
| Xie et al. | In situ fabrication of 3D Ag@ ZnO nanostructures for microfluidic surface-enhanced Raman scattering systems | |
| Jing et al. | Microfluidic device for efficient airborne bacteria capture and enrichment | |
| EP2912189B1 (en) | Devices and methods for manipulating components in a fluid sample | |
| US8373860B2 (en) | Transmitting/reflecting emanating light with time variation | |
| US7386199B2 (en) | Providing light to channels or portions | |
| Voronin et al. | Detection of rare objects by flow cytometry: imaging, cell sorting, and deep learning approaches | |
| US20140220557A1 (en) | Device and Method for Laser Analysis and Separation (LAS) of Particles | |
| JP2007292773A (en) | Microfabricated chemical sensor of diffusion base | |
| CN2821565Y (en) | Single cell algae flow type analysis microflow control chip | |
| EP2638393A2 (en) | Counting particles using an electrical differential counter | |
| Saez et al. | Microfluidics and materials for smart water monitoring: A review | |
| Choi et al. | Highly enriched, controllable, continuous aerosol sampling using inertial microfluidics and its application to real-time detection of airborne bacteria | |
| US20090011430A1 (en) | Electrical detection using confined fluids | |
| CN1715916B (en) | Micro-nano biochemical sensing chip for biochemical molecule detection | |
| CN102680452A (en) | Dual-detection biochemical sensing detector integrated with optofluidics | |
| Blevins et al. | Field-portable microplastic sensing in aqueous environments: A perspective on emerging techniques | |
| Hebert et al. | Label-free detection of Bacillus anthracis spore uptake in macrophage cells using analytical optical force measurements | |
| CN1789970A (en) | Method and apparatus for online measurement of particle size in oil-containing sewage | |
| Chang et al. | Deformation-based droplet separation in microfluidics | |
| CN102735654A (en) | Reflection-type local surface plasma resonance enhanced biochemical detector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110420 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |