CN1715916B - Micro nano biochemical sensitive chip for biochemical molecule detection - Google Patents
Micro nano biochemical sensitive chip for biochemical molecule detection Download PDFInfo
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- CN1715916B CN1715916B CN 200510012233 CN200510012233A CN1715916B CN 1715916 B CN1715916 B CN 1715916B CN 200510012233 CN200510012233 CN 200510012233 CN 200510012233 A CN200510012233 A CN 200510012233A CN 1715916 B CN1715916 B CN 1715916B
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Abstract
The micron and nano biochemical sensitive chip consists of micro mechanical no-valve pump and micron and nanometer biochemical sensor integrated together. It includes mainly liquid inlet, liquid storage pond, micro flow channel,micron and nanormetal structure sensor, right and left flow channels in different resistance, micro mechanical no-valve pump, waste liquid pond, and liquid outlet. Partial biomolecules first attach to the surface of the micron and nano metal structure sensor and then enter the micro mechanical no-valve pump via the right and left flow channels. When the pump cavity volume of the micro mechanical no-valve pump changes periodically, excessive biomolecule liquid will be pumped into the waste liquid pond via the right and left flow channels and be finally exhausted via the liquid outlet from the biochemical sensor chip. The present invention has the advantages of compact structure, small size, high efficiency, etc.
Description
Technical field
The present invention relates to a kind of micro nano biochemical sensitive chip that biochemical molecular detects that is used for.
Background technology
Along with improving constantly of living standards of the people, people have higher requirement to clinical diagnosis and the monitoring and the processing of biochemical compositions such as biomedicine, judicial expertise, food hygiene product supervision analysis, drug development, industry and mining field toxic gas.Traditional biochemical molecular is surveyed identification and mainly is to use radioactive isotope, enzyme or fluorescence etc. as marker, there is the low shortcoming of security and poor stability, efficient and sensitivity, can not satisfy the fast demand of (so that handling and control danger in real time), sensitive (so that surveying extremely toxic substance of trace), special efficacy (getting rid of the interference and the pollution of non-pathogenic composition), urgent wish development high sensitivity of new generation, efficiently, accurately, easily and fast, economic biochemical molecular detection technique.
Summary of the invention
The technical issues that need to address of the present invention are: overcome the deficiency of above-mentioned technology, provide a kind of efficient, high sensitivity, accurately, easily and fast, economical be used for the micro nano biochemical sensitive chip that biochemical molecular detects.
Technical solution of the present invention is: a kind of micro nano biochemical sensitive chip that is used for the biochemical molecular detection, it is characterized in that: micromechanics valveless pump and micro nano biochemical sensitive device are integrated together the formation micro nano biochemical sensitive chip, mainly comprise inlet, liquid storage tank, fluid channel, the metal micro-nano structure sensor, different choked flow road, the left and right sides, the micromechanics valveless pump, waste liquid pool, liquid outlet, biomolecule liquid flows into liquid storage tank by inlet, enter by the metal Nano structure sensor through fluid channel, the part biological molecule can be attached to the surface of metal micro-nanostructure sensor, enter in the micromechanics valveless pump through different choked flow road, a left side again, different choked flow road pumps into waste liquid pool to unnecessary biomolecule liquid through the right side when the pump chamber generating period of micromechanics valveless pump changes, after liquid outlet is discharged biochemical sensitive chip.
The metal micro-nano structure sensor is a cycle metal nano dot matrix, adopts micro-nano process equipment (as the beamwriter lithography machine) to make, and the minimum feature size of cycle metal nano dot matrix is below 100 nanometers, and the cycle is hundred nanometer scale.
Different choked flow road can adopt the Tesla runner of Nicola Tesla invention.The principle of work in different choked flow road is: when forward flow, fluid flows through the sprue that width is big, bending angle is less, total length is less, and its flow resistance is very little; When reversed flow, the direction that fluid will at first be selected owing to inertia and original momentum orientation angle is less is advanced, so quite a few fluid will enter the less side runner of width.The length of side runner is greater than sprue, and bending angle is also greater than sprue, and sectional area is less than sprue.The Way out of side runner will be designed to and the angle of sprue working direction formation greater than 90 °, thereby constitute the resistance that the sprue fluid is advanced.
Principle of the present invention is: different choked flow road relies on its different flow resistance to play movement-oriented effect, when the pump chamber of micromechanics valveless pump sucks liquid, liquid in the different choked flow road, a left side flows along positive dirction, liquid in the right different choked flow road flows along negative direction, because the directional selectivity in different choked flow road, the fluid flow that flows into pump chamber from the left side is greater than the flow that flows into from the right side.When the pump chamber discharged liquid, the liquid in the left different choked flow road flows along negative direction, and the liquid in the right different choked flow road flows along positive dirction, and in like manner, the fluid flow that flows out pump chamber from the right side is greater than the flow that flows out from the left side.Through such work period, with a part of flow pump in left side to the right side.The light that light source sends is used to excite the non-radiative surface plasma in the metal film, macromolecular absorption and desorption have changed local indexes of refraction on the sensor surface, the wave vector of surface plasma changes, when light under the condition of the wave vector coupling of the wave vector component on sensing unit surface and surface plasma, resonance causes the energy loss in reflected light or the transmitted light.
The present invention has following advantage than prior art:
(1) micromechanics valveless pump and micro nano biochemical sensitive device are integrated together the formation micro nano biochemical sensitive chip, have compact conformation, little, the efficient advantages of higher of volume, can be used for the control of express-analysis and level process.
(2) the reciprocal valveless pump of micromechanics is simple in structure, and can be designed to plane figure, is particularly suitable for adopting fine process to make.
(3) the surface plasma sensing unit among the present invention can be used to recycling.In contrast, traditional immunoassays comprise the immunosorbent chemical examination that enzyme interrelates, and can only use once basically.
(4) traditional biochemical molecular detection identification mainly is to use radioactive isotope, enzyme or fluorescence etc. as marker, has the low shortcoming of security and poor stability, efficient and sensitivity.The present invention utilizes the good electric magnetic property and the bioaffinity of metal micro-nanostructure that biochemical molecular is detected, and can satisfy the demand of (so that handling and control danger in real time), sensitive (so that surveying the extremely toxic substance of trace), special efficacy (getting rid of the interference and the pollution of non-pathogenic composition) fast.
The present invention can realize new biochemical sensitive Detection Techniques, for establishing new technical foundation in fields such as dna molecular, protein demarcation and identification, drug delivery, molecular device, virus detection, bacterium detection, microstructure diagnosis and active treatment.
Description of drawings
Fig. 1 is a structured flowchart of the present invention;
Fig. 2 is the fundamental diagram of micromechanics valveless pump of the present invention;
Fig. 3 is the structural drawing of metal micro-nanostructure sensor of the present invention;
Fig. 4 is a course of work synoptic diagram of the present invention.
Embodiment
As shown in Figure 1, the present invention is by inlet 1, liquid storage tank 2, fluid channel 3, metal micro-nano sensor 4, different choked flow road, the left and right sides 7, micromechanics valveless pump 8, waste liquid pool 9, liquid outlet 10 is formed, biomolecule liquid flows into liquid storage tank 2 by inlet 1, enter the metal micro-nano sensor 4 that constitutes by metal micro-nanostructure 5 through fluid channel 3, part biological molecule 6 can be attached to the surface of metal micro-nanostructure 5, when the pump chamber generating period of micromechanics valveless pump 8 changes, as shown in Figure 2, different choked flow road 7 flows into waste liquid pool 9 to unnecessary biomolecule liquid through the left and right sides, after liquid outlet 10 is discharged biochemical sensitive chip.Wherein micromechanics valveless pump 8 can be designed to plane figure, and this structure is the simplest, be convenient to processing, is particularly suitable for adopting fine process to make.
As shown in Figure 3, the metal micro-nanostructure sensor is a cycle metal nano dot matrix, adopts micro-nano process equipment (as the beamwriter lithography machine) to make on metal (as silver) film, and the minimum feature size of dot matrix is below 100 nanometers, cycle is 100-900 nanometers, i.e. hundred nanometer scale.
As shown in Figure 4, the light that light source sends has on the metal micro-nanostructure sensor of biomolecule and produces surface plasma wave through shining surface attachment after the collimated, when light under the condition of the wave vector coupling of the wave vector component on sensing unit surface and surface plasma, resonance causes the energy loss of reflected light or transmitted light, and this is a tangible low ebb in reflectance spectrum or transmitted spectrum.Reflectance spectrum or transmitted spectrum are converted to electric signal by CCD with light signal and send into computer processing system analysis through being imaged on behind the spectrometer on the CCD.
If light source is a white light, by the detectable biomolecule of the variation of absorption spectrum.If light source is a monochromatic light, the resonance angle depends on the refractive index profile of the interior sample of evanescent wave scope on the sensor surface consumingly.Macromolecular absorption and desorption have changed local indexes of refraction on the sensor surface, make the resonance angle take place one and move, and this moves relevant with the kind of biochemical molecular.
Claims (3)
1. one kind is used for the micro nano biochemical sensitive chip that biochemical molecular detects, it is characterized in that: micromechanics valveless pump and metal micro-nano structure sensor is integrated to constituting micro nano biochemical sensitive chip together, mainly comprise inlet, liquid storage tank, fluid channel, the metal micro-nano structure sensor, different choked flow road, the left and right sides, the micromechanics valveless pump, waste liquid pool, liquid outlet, biomolecule liquid flows into liquid storage tank by inlet, enter the metal Nano structure sensor through fluid channel, the part biological molecule can be attached to the surface of metal micro-nano structure sensor, enter in the micromechanics valveless pump through different choked flow road, a left side again, when the pump chamber generating period of micromechanics valveless pump changes, different choked flow road pumps into waste liquid pool to unnecessary biomolecule liquid through the right side, after liquid outlet is discharged biochemical sensitive chip; Described different choked flow road is the Tesla runner.
2. the micro nano biochemical sensitive chip that is used for the biochemical molecular detection according to claim 1, it is characterized in that: the cycle metal nano dot matrix of described metal micro-nano structure sensor for adopting the micro-nano process equipment to make, the minimum feature size of cycle metal nano dot matrix is below 100 nanometers, and the cycle is hundred nanometer scale.
3. the micro nano biochemical sensitive chip that is used for the biochemical molecular detection according to claim 1, it is characterized in that: described micromechanics valveless pump is designed to plane figure, adopts fine process to make.
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| CN 200510012233 CN1715916B (en) | 2005-07-21 | 2005-07-21 | Micro nano biochemical sensitive chip for biochemical molecule detection |
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| CN 200510012233 CN1715916B (en) | 2005-07-21 | 2005-07-21 | Micro nano biochemical sensitive chip for biochemical molecule detection |
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| CN1715916A CN1715916A (en) | 2006-01-04 |
| CN1715916B true CN1715916B (en) | 2011-04-20 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI476406B (en) * | 2011-11-10 | 2015-03-11 | Apex Biotechnology Corp | Reaction cassette and assay device |
| CN105051538B (en) * | 2012-12-17 | 2018-06-15 | 伦珂德克斯有限公司 | For detecting the system and method for biological condition |
| CN104049029B (en) * | 2014-07-02 | 2017-05-10 | 重庆大学 | Multi-channel magneto-elastic sensor detection chip |
| CN104502303B (en) * | 2015-01-20 | 2017-05-31 | 中国人民解放军第三军医大学第一附属医院 | For the Asia-Pacific hertz nano biological sensor and its detection method of fast frame inspection bacterium |
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Non-Patent Citations (1)
| Title |
|---|
| 傅新等.集成微泵式微流控芯片的设计与测试.《2003年中国机械工程学会年会》.2003,102-111. * |
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