CN101031500A - Micro-fluid device - Google Patents
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- CN101031500A CN101031500A CN 200580032736 CN200580032736A CN101031500A CN 101031500 A CN101031500 A CN 101031500A CN 200580032736 CN200580032736 CN 200580032736 CN 200580032736 A CN200580032736 A CN 200580032736A CN 101031500 A CN101031500 A CN 101031500A
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Abstract
A closed loop microfluidic device that has at least one microchannel (74) formed in a body and a pump (72) in fluid connection with the microchannel. The pump is actuated by an external motive force to push and pull fluid through the microchannel. A number of chambers (73, 76) are formed in fluid connection with the microchannel to store reagents. The reagents are moved through the microchannel by the pump. A number of active zones (70, 71) are also formed in the microchannel. Various reactions and diagnostics are performed at the active zone. A sample is introduced to the microchannel through a sealable input port (77). The microchannel forms a closed loop with all necessary reagents and diagnostics contained within the closed loop microfluidic device. The sample is processed and analysed completely within the closed loop microchannel.
Description
Technical field
The present invention relates to a kind of microfluidic device.Specifically, the present invention relates to a kind of closed-loop device, it comprises the one or more pumps that are used for moving around loop fluid sample.This device especially is applied to small-sized biologicall test chip.
Background technology
Research and development to the biometric apparatus design have recently concentrated on microfluid, and just small volumes of sample and reactant are around the mobile aspect of fluid channel.A kind of like this device has been described in the U.S. Patent application 2004/0132218 of Ho.Ho has described a kind of small-sized biologicall test chip design of complexity, and it has the reagent cavities of a plurality of many observation wells and a plurality of sealings.Biochip has little lid arrangement, and described little lid arrangement pierces through the sealing of reagent cavities so that reactant is discharged in the observation well.The Ho device cannot be used for micropump and be confined to thus quite simply use.
The system that Kuo describes in U.S. Patent application 2003/0233827 feasible reactant quantitative aspects simpler but comprise the diaphragm micropump and thus can be between the zone on the microchip mobile sample and reactant.Identical with many microchip systems, Kuo is difficult to make fluid to move around chip because of forming vacuum in the fluid back of moving.For this reason, Kuo has the system of partially opening.Open system and be not suitable for most biometric applications, in particular for longer-term storage or relate to the application of dangerous calibrating (carcinogenic substance etc.).
Singh has described the most comprehensive description of (feasible) available system in the patent families that comprises U.S. Patent application 2002/0098122 and International Patent Application WO 02/057744.Singh has described and a kind ofly sample has been housed and is placed on replaceable microfluid biochip in the read-out device.This biochip has and a plurality ofly carries out mangneto moving check-valves and diaphragm pump by the electromagnet in the read-out device.Because adopt static electromagnetic iron and check-valves, Singh has limited the versatility of biochip.
Kamholz has described a kind of pump of effective form in U.S. Patent No. 6408884 and No.6415821, and has listed many reference papers therein.Kamholz has described a kind of employing magnetic field and has moved limonite ferrogel along fluid channel) piece is so that before described or the ferrofluidic pump that moves of the fluid of back.Kamholz only discloses out device and has at least one fluid intake and at least one fluid issuing, thereby makes fluid flow through this device.Kamholz does not disclose closed-loop device.
Transferring to have described among the U.S. Patent application No.5096669 of I-Stat Corporation a kind ofly is used to adopt hand-held read-out device and disposable microchip to carry out the system of fluid analysis.But described microchip adopts capillarity to suck sample in the chip and sucks the step-down bubble to impel the sample flows through sensor.This I-Stat device is not closing device and is unsuitable for longer-term storage.Described design only can be carried out the simple of fluid and move.
In the International Application No. WO 2003/035229 that transfers NTU Ventures Pte Ltd., another kind of design has been described.This NTU device is flow type rather than closed loop design.For add and remove sample, buffer, flowing promotes fluid etc. and has a plurality of entrance and exits.Even some reactants are stored on the device in advance, this NTU device also needs user's continuous interactive to do in order to carry out diagnostic test.Described device also needs arrangement of valves in case fluid stopping is gone in the unnecessary passage and chamber.
Transferring Motorola Inc, U.S. Patent application No.2005/0009101 in a kind of microfluidic device that a plurality of collections bonding ligands position is housed has been described.The Motorola patent application has been described the recirculation of employing valve control sample repeatedly by bonding position, with main raising signal strength signal intensity.Valve is combined in has increased complexity and cost on the microfluidic device.
A kind of passive substantially microfluidic device has been described in transferring the U.S. Patent application No.2004/0248306 of Hewlett-Packard Company.This Hewlett-packard device relies on capillarity to make fluid sample flow through device fully.In order to make capillarity effective, need air management chamber.Rely on versatility and validity that capillarity has seriously limited device.
In the International Application No. WO 1999/49319 of Streen Ostergard and Gert Blankenstein, can find another the attractive application of fluid channel technology.Their device is " non-current " micro sprue system that utilizes field mobile particle between activating area.A kind of example is to make sample and be bonded in the reactant interaction on the magnetic bead and utilize magnetic field to make described pearl move through passage, and thus by buffer and reactant.
Although have multiple available microfluidic device, but still a kind of device need be provided, wherein under having need not situation that the user intervenes after the gatherer, sample can finish all necessary treatment steps that carry out sample analysis.
Summary of the invention
The purpose of this invention is to provide a kind of closed loop microfluidic device.
From following description, will be well understood to other purpose.
(however its not necessarily unique or be actually the most wide in range form) in one form the present invention relates to a kind of closed loop microfluidic device, comprising:
Main body:
At least one fluid channel that on main body, forms, described fluid channel forms closed circuit;
At least one sealable input port is used for sample delivery in described at least one fluid channel; And
With at least one pump that described at least one fluid channel fluid is communicated with, described pump receives external motive force.
Preferably, described device also comprises at least one collecting zone that is positioned in the main body and is communicated with described at least one fluid channel fluid.
Described device preferably also comprises at least one surveyed area that is positioned in the main body and is communicated with described at least one fluid channel fluid.Described surveyed area and collecting zone suitably can be the single zones of realizing two functions.
Can have at least a reactant, it is contained in the interior chamber of main body and can moves through described at least one fluid channel under the effect of pump.
Suitably, described pump is that ferrofluidic pump and described external motive force are magnetic field.Described pump applies power and passes through fluid channel with pulling and propelling fluid.
Described device preferably has a plurality of fluid channel that described salable input port is linked to each other with one or more chambeies and one or more zone.
In another form, the present invention relates to a kind of method of in closed loop microfluidic device, handling sample, may further comprise the steps:
Be drawn in the fluid channel that forms on apparatus main body by the described sample of input port with metered amounts, described fluid channel forms closed circuit;
The sealing input port is with the described device of closure; And
Apply external motive force so that sample moves to from the input port at least one activating area to pump, described pump applies power and passes through fluid channel with pulling and promotion sample.
Description of drawings
In order to help to understand the present invention, now with reference to accompanying drawing preferred implementation is described, wherein:
Fig. 1 is the schematic diagram of expression closed loop microfluidic device operating principle;
Fig. 2 is that the expression sample imports the schematic diagram in the closed loop microfluidic device with a zone of first embodiment;
Fig. 3 represents that sample moves into described zone;
Fig. 4 represents that sample moves through described zone;
Fig. 5 represents to be contained in the reactant in the device;
Fig. 6 represents that reactant moves through described zone;
Fig. 7 is the schematic diagram of the closed loop microfluidic device of second embodiment;
Fig. 8 is the cross sectional representation along this embodiment of the intercepting of the AA among Fig. 7;
Fig. 9 represents that view shown in Figure 8 has the predeformation pressure texture;
Figure 10 presentation graphs 9 illustrated embodiment sample of packing into;
Figure 11 represents to have the 3rd embodiment of the closed loop microfluidic device of two fluid channel loops;
Figure 12 represents that fluid sample moves around device shown in Figure 11 under the effect of first pump;
Figure 13 represents that fluid sample moves around device shown in Figure 11 under the effect of second pump;
Figure 14 represents that fluid sample moves around device shown in Figure 11 once more under the effect of first pump;
Figure 15 represents the sketch of biologicall test chip;
Figure 16 represents the detailed maps of a kind of embodiment of biologicall test chip;
Figure 17 represents the image of biologicall test chip read-out device;
Figure 18 represents the schematic diagram of biologicall test chip read-out device operation;
Figure 19 represents the first step of biologicall test chip operation shown in Figure 16;
Figure 20 represents second step of chip operation shown in Figure 16;
Figure 21 represents the third step of chip operation shown in Figure 16;
Figure 22 represents the 4th step of chip operation shown in Figure 16;
Figure 23 represents the 5th step of chip operation shown in Figure 16;
Figure 24 represents the first step of the biologicall test chip operation of second embodiment;
Figure 25 represents second step of chip operation shown in Figure 24; And
Figure 26 represents the third step of chip operation shown in Figure 24.
The specific embodiment
When describing the present invention's difference embodiment, shared Reference numeral is used to indicate identical parts.
With reference to Fig. 1, show the schematic diagram of the microfluidic device 10 that comprises main body 11 and closed loop microchannel 12.Pump 13 makes fluid sample 14 move around loop.Because described fluid channel is a closed loop, so pump can promote and spur sample as shown by arrows.
As shown in Figure 2, liquor sample 14 can be by comprising inlet 15a when pump 13 stops, and the token import mouth 15 of 15b imports in the fluid channel 12.The sample fluid that prevents the pump that stops to move and flows through except that inlet 15a, the fluid channel outside between the 15b.Liquor sample injects a mouth such as in the 15a, the air in the fluid channel is discharged by another inlet 15b.Can be not more than inlet 15a owing to import the volume of sample, the volume of the fluid channel between the 15b, therefore this structure can make the liquor sample of metered amounts import in the microfluidic device.
In case fluid sample 14 has been packed in the fluid channel 12, inlet 15a then, 15b be the lid 16a by as shown in Figure 3 for example, and 16b obtains sealing.Pump 13 activates sample 14 is for example moved to activating area 17 by fluid channel.
In case will recognize that inlet 15a, 15b utilizes and covers 16a, and 16b obtains sealing, and then described device is closed fully.This point is advantageous particularly under described device is used to carcinogenic or situation that the pathogeny sample is measured.Yet this device not necessarily must be used for this purposes.It can be specially adapted to long term storage of biological samples.In case sample imports microfluidic device, then it can avoid polluting for a long time.The preferred implementation of this device by can be stored in absolute zero or in its vicinity and the medical plastic under the vacuum constitute.The inventor believes that this device is highly suitable for for example longer-term storage of blood of Biosample.
As mentioned above, preferred implementation shown in Figure 2 comprises it being the activating area 17 of storage area in one embodiment.For longer-term storage, sample 14 can remain on regional 17 places, but usually preferably, pump 13 makes sample 14 continue to move through zone 17, as shown in Figure 4, thereby important component 18 is retained in regional 17 places.Zone 17 is considered to be used for collecting and keeping from sample 14 collecting zone of important component 18 in this case.These important component can be in unlimited time memory storage in closed loop microfluidic device.
Embodiment shown in Fig. 2-4 makes sample can store and make important component can extract and obtain storage for a long time from sample.The inventor believes that this device will be applied to storage of blood, extract blood constituent to store and to store natural and synthetic extract.Sample can be included in for example genetic typing of subsequent analysis, evaluation or legal medical expert and examine and determine the nucleic acid that is collected in the analysis and avoids degrading.This device is specially adapted to the hereditary evidence that uses in the longer-term storage criminal case.
In many application, will need to utilize the reactant that carries in the microfluidic device 10 to handle sample.Embodiment shown in Figure 5 shows that reactant 19 can be placed in the fluid channel 12 before sample 14 imports.From being well understood to preceding argumentation, sample 14 can not pass through inlet 15a under the situation of disturbance reactant 19 when pump 13 stops, and 15b imports and also is locked in the appropriate location.In case inlet 15a, 15b obtain sealing and pump 13 obtains activating, then sample 14 moves through fluid channel 12.Reactant 19 also moves through fluid channel 12 with identical speed.As shown in Figure 6, important component 18 is collected in regional 17 places and is washed by reactant 19.The continuation of pump 13 operation will make reactant 19 move through important component 18 and arrive near the position the pumps 13 and sample 14 moved to inlet 15a, near the position the 15b.
Fig. 7 represents to comprise second embodiment of the microfluidic device 20 of main body 21 and closed loop microchannel 22.Pump 23 makes fluid sample 24 move through zone 27 around loop 22.
In embodiment shown in Figure 7, sample 24 injects in the fluid channel 22 in the distortion of pressure contained structure.Fig. 9 represents pressure contained structure 26 predeformation and can be used as the distortion embodiment of sucker mechanism.The user fills inlet 25 and structure 26 is released (manually or automatically) so that sample 24 is sucked in the cavity 28, as shown in figure 10.
Disclosed total operating principle can be applied to more complicated structure among Fig. 1-10.Figure 11 represents to comprise the embodiment of the microfluidic device 50 of double loop fluid channel 52, and described fluid channel 52 comprises first loop 52a with pump 53 and the second loop 52b with pump 54.First fluid piece 55 is positioned at the first loop 52a and second bulk fluid 56 is positioned at the second loop 52b.These bulk fluid can be by the sample of a kind of importing in the said method or can be pre-aligned reactants in loop.
When second pump 54 stops and first pump 53 when activating, first fluid piece 55 is driven through loop 52a as shown by arrows.Piece 55 will move around loop as shown in figure 12.Owing to compare with the pressure among the second loop 52b, pump 53 produces higher pressure and produces lower pressure in front in the back of piece 55, so piece 55 can not move in the second loop 52b.
As shown in figure 13, can second bulk fluid 56 be moved around loop 52b by disconnecting first pump 53 and activating second pump 54.Will recognize that any one pump can make bulk fluid move through common microchannel between the loop.In case first fluid piece 55 has moved in the second loop 52b, then second pump 54 can stop and first pump 53 activates again, but in the opposite direction.Will propel fluid slug 56 enter the first loop 52a like this, as shown in figure 14.
Continued operation shown in Figure 11-14 shows how closed loop microfluidic device is used at the situation lower-pilot fluid sample that need not any moving-member (under the situation in ferrofluidic pump) or mechanical valve.Device (all will fall within the scope of the present invention) mobile fluid sample that can complex structure and reactant with collect, complex process and analysis.
In Figure 15, schematically shown complex bioassay chip with chamber.This biologicall test chip totally is denoted as 60, and comprises the plastic body 61 that wherein forms a plurality of passages 62 and chamber 63.Hereinafter passage and chamber effect are separately described in more detail, as shown in figure 17 with reference to the operation that combines the chip 60 of chip read-out device 80.In some embodiments, connector 64 transmits the signal of telecommunication between chip 60 and read-out device 80.
Figure 16 illustrates a kind of detailed layout schematic diagram of biologicall test chip of embodiment.Chip is configured to analyze little chemistry or Biosample to detect one or more target substances in this embodiment.This chip is configured to comprise magnetic capture zone 70 and electricity activation surveyed area 71, and described surveyed area 71 is the arrangement of electrodes that detects the signal in the charged particle that is discharged by collecting zone in this embodiment.First ferrofluidic pump 72 makes the solution from first chamber 73 move through a plurality of passages for example 74.Second ferrofluidic pump 75 makes another solution from second chamber 76 move through a plurality of passages.Sample is in mouth 77 places import chip 60.
Described biologicall test chip comprises a plurality of passive chocking constructions, thereby reactant is contained in each chamber.In general, the cross sectional dimensions of chocking construction minimum is littler than the cross sectional dimensions of second channel minimum, be enough to make the capillary force differ to prevent to utilize capillarity to pass through chocking construction, and in second channel, suck in the second channel during without any fluid from the fluid of first passage.
Be known in the art that ferrofluidic pump is dripped formation by the ferrofluid that moves under the effect in magnetic field.In a preferred embodiment, magnetic oil droplet 72a, 75a at chamber 72b, moves in the 75b under the effect of the impressed field that is for example produced by the magnet that moves.
Hereinafter chip 60 is described in more detail with reference to concrete application.As mentioned above, chip 60 operations are as closed-loop system.In case sample imports in the chip 60, then sample is contacted without any the outside.Ferrofluid pump operated so that sample and solution move and gather signal by connector around chip.
Chip read-out device 80 has the room 81 that holds chip 70.Connector 64 is aimed at corresponding connector 82 in the read-out device.When door 83 is closed, can on display 84, obtain the menu of the test that can carry out and can adopt button 85 to select.When finishing test, discharge by pushing button 86 with the chip of crossing 60.Inventor's expectation can be replaced chip 60, however can expect utilizing the chip that can reuse.
Figure 18 represents the schematic block diagram of the building blocks of function of chip read-out device 80.The read-out device center is digital signal processor or other processing member 90.In this member, finish all control and analytic process.Although be illustrated as single member, it will be recognized by those skilled in the art usually provides described function by a plurality of integrated circuits and separate member.A pair of actuator 91,92 provides and makes oil droplet 72a, and 75a is along chamber 72b, the motive power that 75b moves.In a kind of simple embodiment, actuator is to measure linearly moving magnet under the chip 60.Can also switch on and produce magnetic field.Can expect the motion more complicated than simple linear movement.Signal from surveyed area 71 enters DSP 90 via connector 64 and 82.Can on display 84, obtain test result.Read-out device can also have and is used for linking to each other to carry out the external access port (not shown) of off-line analysis in more detail with computer.
As mentioned above, read-out device and chip are not limited to any particular detection method.Read-out device can comprise other selectable checkout gear, and for example photodiode 93.Signal is directly read by read-out device and without any need for connector 64,82 in this embodiment.
In order more to be expressly understood the operation of measuring chip 60, a kind of concrete example is described with reference to the chip layout shown in Figure 19-23.Chip 60 initially is filled with buffer agent solution 100 and the detergent solution in cleaning agent chamber 76 101 in buffer chamber 73.Oil droplet 72a, 75a are contained in pump chamber 72b respectively, in the 75b.
In use, from the test event of read-out device is single, choose a kind of test.Prepare sample 102 by mixed for several minutes in the test bottle with indication kind of (reporter species) and magnetic bead, described indication kind and magnetic bead all are coated with chemistry or the biological acceptor that can discern and collect the analyte in the sample.This analyte is collected between magnetic bead and the indication kind.Suitable indication kind is including, but not limited to dendron matrix (dendrimers), latex bead, liposome, collaurum, fluorescent material, visual material, biological and chemical luminescent material, enzyme, nucleic acid, peptide, protein, antibody and aptamer (aptamer).Acceptor can be biological cell, protein, antibody, peptide, antigen, nucleic acid, aptamer, enzyme or other biological acceptor and chemoreceptor.
In a preferred embodiment, it is the liposome of filling a large amount of labelled molecule that indication is planted, and makes each analyte molecule carry a large amount of labelled molecule indirectly this moment, and it falls after having the liposome dissolving of lytic agent and obtains discharging, thereby causes direct signal to amplify.Remain in label suitable in the liposome and comprise fluorescent dye, visible dyes, biological and chemical luminescent material, enzyme substrate, enzyme, radioactive material and electric activating material.Suitable lytic agent comprises surfactant for example octyl glucopyranose, dodecyl sodium sulfate, sodium dioxide cholate, tween-20 and trinitrotoluene X-100.Alternatively, can adopt complement lysis.
Will recognize that other gathering system that can adopt outside the magnetic bead and concrete preparation will depend on test characteristic and sample character.In addition, the present invention is not limited to any specific test configuration and comprises direct and indirect competitiveness and noncompetitive mensuration.The inventor expects that available test specification can enlarge along with the time.Yet, set concrete preparation method of sample in order to illustrate.
In the sample 102 addings mouth 77 as shown in figure 19.Apply and compress (104) lid 103 to force sample 102 to insert sample chamber 106 by path 10 5.Unnecessary sample is inserted waste chamber 107 to discharge air by exhaust outlet 108.The mensuration chip 60 that exhaust outlet 108 is closed and sealed is arranged in the read-out device 80.
When first magnetic actuator still worked, second magnetic actuator 92 in the read-out device 80 activated to drive oil droplet 75a along chamber 75b, forced detergent solution 101 from chamber 76 in the flow channel 120 (Figure 21) thus.When passage 120 was full of cleaning agent, magnetic actuator 91 stopped.Therefore cleaning agent 101 flows to zone 70.When cleaning agent 101 reached magnetic capture zone 70, cleaning agent made the quick-fried spray of liposome (burst) (Figure 22).Electricity activation charged particle 112 is by electrode 71 regurgitates and produce diagnostic signal (Figure 23).This signal is received by connector 64 and connector 82 by the DSP in the read-out device 80 90.
The operation of ferrofluidic pump 72,75 is very crucial to the operation of measuring chip constantly.Second pump 75 is starting before the stroke of first pump 72 finishes just.Guarantee to make the danger of bringing bubble into to reduce like this.Pump 72 is still in operation simultaneously for cleaning agent admission passage 131, and some cleaning agents flow to the buffer back and collect bubble 132 thus, as shown in figure 22.When pump 72 stopped, the continuation of pump 75 operation forced cleaning agent 101 to cross collecting zone 70.
Although preferred implementation adopts two ferrofluidic pump, will recognize that the present invention is not limited thereto.Figure 24 is the structure chart that adopts the chip 200 of single ferrofluidic pump 210.In addition, chip is not limited to detect electric activating substance.Embodiment shown in Figure 24 adopts photoelectric detecting technology, wherein by the photodiode in the read-out device 93 it is detected during by window 212 at photosensitive sample.
Identical with first embodiment, chip is equipped with buffer 201 and reactant 202 in advance.Sample 203 is produced and imports in the mouth 204.Sample is inserted in the bubble trap 205 when lid 207 is exerted pressure, and unnecessary sample enters waste chamber 206.Exhaust outlet 208 is closed and exhaust outlet 209 is opened, as shown in figure 25.Ferrofluidic pump 210 activates with by passage 221 pump buffer 201, forces sample 203 to cross collecting zone 211 thus and enters in the waste chamber 222, as shown in figure 25.Simultaneously, reactant 202 is inhaled in the chocking construction 224.
Passage is for example 220 enough little of there to be suitable surface tension.Therefore as long as exhaust outlet 209 is opened, sample 203 and buffer 201 will flow in the waste chamber 222.
Can estimate, need other ferrofluidic pump design in concrete the application.
The application of the microfluidic device that is used for electro-detection and photodetector system has been described.Will recognize that the present invention is not limited to any particular detection system, in fact as described in the preceding, described device can only be used for storage and be not used in any detection system.To recognize that also the present invention is not limited to specific fluid channel quantity or structure.Have one or two fluid channel loop although described embodiment is described as, it will be apparent for a person skilled in the art that the present invention can expand to a plurality of loops of fluid connection to some extent.
The purpose of whole specification is to describe preferred implementation of the present invention rather than will limit the present invention to any embodiment or concrete feature combination.
Claims (40)
1. closed loop microfluidic device comprises:
Main body:
At least one fluid channel that in main body, forms, described fluid channel forms closed circuit;
At least one sealable input port is used for sample delivery in described at least one fluid channel; And
With at least one pump that described at least one fluid channel fluid is communicated with, described pump receives external motive force.
2. closed loop microfluidic device according to claim 1 is characterized in that, also comprises the one or more activating areas that are positioned at main body and are communicated with described at least one fluid channel fluid.
3. closed loop microfluidic device according to claim 2 is characterized in that, at least one in described one or more activating areas comprises the storage area that is suitable for storing sample.
4. closed loop microfluidic device according to claim 2 is characterized in that, at least one in described one or more activating areas comprises the collecting zone that is suitable for collecting one or more sample constituents.
5. closed loop microfluidic device according to claim 2 is characterized in that, at least one in described one or more activating areas comprises the surveyed area that is suitable for detecting one or more sample constituents.
6. closed loop microfluidic device according to claim 2, it is characterized in that, in described one or more activating area at least one comprises the collecting zone that is suitable for collecting sample or sample constituents, and in described one or more activating area at least one comprises the surveyed area that is suitable for detecting one or more sample constituents.
7. closed loop microfluidic device according to claim 1 is characterized in that, also comprises the one or more chambeies that are positioned at main body and are communicated with described at least one fluid channel fluid.
8. closed loop microfluidic device according to claim 7 is characterized in that, at least one in described one or more chambeies held at least a reactant that can move through described at least one fluid channel under the effect of pump.
9. closed loop microfluidic device according to claim 1 is characterized in that described pump is a ferrofluidic pump, and described external motive force is magnetic field.
10. closed loop microfluidic device according to claim 1 is characterized in that, comprises a plurality of fluid channel that described salable input port is linked to each other with one or more chambeies and one or more activating area.
11. closed loop microfluidic device according to claim 1 is characterized in that, described salable input port is arrived the sample delivery of metered amounts in described at least one fluid channel.
12. closed loop microfluidic device according to claim 1 is characterized in that, also comprises the pressure contained structure that is communicated with described salable input port fluid, this pressure contained structure absorption pressure in sample is transported to described at least one fluid channel the time.
13. closed loop microfluidic device according to claim 1 is characterized in that, also comprises the sucker mechanism that is communicated with described salable input port fluid, this sucker mechanism sucks sample in described at least one fluid channel.
14. closed loop microfluidic device according to claim 1 is characterized in that, also comprises one or more salable waste material mouths.
15. closed loop microfluidic device according to claim 2 is characterized in that, at least one in described one or more activating areas is the electrode that detects from the signal of described sample.
16. closed loop microfluidic device according to claim 2 is characterized in that, at least one in described one or more activating areas is magnetic capture zone.
17. closed loop microfluidic device according to claim 1 is characterized in that, also comprises data link.
18. closed loop microfluidic device according to claim 2 is characterized in that, at least one in described one or more activating areas is the Photoelectric Detection zone, and it detects the signal from the photosensitive particle in the described sample.
19. closed loop microfluidic device according to claim 1 is characterized in that, comprises two continuous fluid channel, a pump is communicated with each fluid channel fluid.
20. closed loop microfluidic device according to claim 19 is characterized in that, comprises at least two chambeies that are positioned at main body, a chamber is communicated with each fluid channel fluid, and one of them chamber comprises buffer agent solution and a chamber comprises cleaning agent.
21. a method of handling sample in closed loop microfluidic device may further comprise the steps:
Be drawn in the fluid channel that forms on apparatus main body by the described sample of input port with metered amounts, described fluid channel forms closed circuit;
The sealing input port is with the described device of closure; And
Apply external motive force to pump, so that sample moves at least one activating area from the input port, described pump applies power and passes through fluid channel with pulling and promotion sample.
22. method according to claim 21 is characterized in that, and is further comprising the steps of:
Collect sample at first activating area; And
The reactant of pre-stored is handled sample in the use device.
23. method according to claim 22 is characterized in that, also comprises external motive force is activated reactant is moved to the step of first activating area from the storage chamber that prestores.
24. method according to claim 21 is characterized in that, and is further comprising the steps of:
Collect sample at first activating area;
The reactant of pre-stored is handled sample in the use device; And
At the second activating area analytical sample.
25. method according to claim 24 is characterized in that, also comprises external motive force is activated, so that reactant is moved to first activating area and sample moved to the step of second activating area from first activating area from the storage chamber that prestores.
26. method according to claim 24 is characterized in that, described first activating area is a collecting zone, and second activating area is a surveyed area.
27. method according to claim 24 is characterized in that, described first activating area becomes to collect and surveyed area with the second activating area alignment by union.
28. method according to claim 21 is characterized in that, also comprises making external motive force oppositely so that the reverse step of the moving direction of sample.
29. method according to claim 21 is characterized in that, thereby also comprises to second pump and apply external motive force so that reactant moves through the step of second fluid channel and described specimen reaction.
30. method according to claim 29 is characterized in that, also comprises to described pump or described second pump applies external motive force so that reactant moves to the step of activating area from the chamber.
31. method according to claim 29 is characterized in that, also comprises successively to described pump and described second pump applies external motive force so that described sample and reactant move through the step of described fluid channel and described second fluid channel in a controlled manner.
32. a closed loop microfluidic device comprises:
Main body;
First fluid channel that in main body, forms, described fluid channel forms closed circuit;
Second fluid channel that in main body, forms and be communicated with the first fluid channel fluid, described second fluid channel forms closed circuit;
The one or more chambeies that are communicated with the first fluid channel fluid;
The one or more chambeies that are communicated with the second fluid channel fluid;
Be used for sample delivery to described first or of second fluid channel at least one salable input port;
Be communicated with the first fluid channel fluid and receive external motive force so that fluid moves through first pump of described first fluid channel;
Be communicated with the second fluid channel fluid and receive external motive force so that fluid moves through second pump of described second fluid channel; And
The one or more activating areas that are arranged in main body and are communicated with at least one fluid of described first or second fluid channel.
33. closed loop microfluidic device according to claim 32 is characterized in that, is included in the buffer storage chamber in described first fluid channel.
34. closed loop microfluidic device according to claim 32 is characterized in that, is included in the cleaning agent storage chamber in described second fluid channel.
35. closed loop microfluidic device according to claim 32 is characterized in that, described first fluid channel and described second fluid channel have shared runner section.
36. closed loop microfluidic device according to claim 35 is characterized in that, is included in the collecting zone in the described shared runner section.
37. closed loop microfluidic device according to claim 36 is characterized in that, described collecting zone is a magnetic capture zone.
38. closed loop microfluidic device according to claim 35 is characterized in that, is included in the surveyed area in the described shared runner section.
39., it is characterized in that described surveyed area comprises the electrode of detection from the signal of described sample according to the described closed loop microfluidic device of claim 38.
40. a read-out device that is used for closed loop microfluidic device, described closed loop microfluidic device comprises:
Main body;
At least one fluid channel that in main body, forms, described fluid channel forms closed circuit;
Be used for the salable input port of sample delivery in described at least one fluid channel;
With at least one pump that described at least one fluid channel fluid is communicated with, described pump receives external motive force; And
The one or more activating areas that are positioned at main body and are communicated with described at least one fluid channel fluid;
Described read-out device comprises:
The room that holds described closed loop microfluidic device;
One or more actuators of described external motive force are provided;
With at least one signal communication in described one or more activating areas and therefrom receive the connector of the signal of the described sample of expression; And
The signal of the described actuator of control is provided and analyzes the signal that receives from described connector to characterize the signal processor of described sample.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2004905578 | 2004-09-28 | ||
| AU2004905578A AU2004905578A0 (en) | 2004-09-28 | Closed-loop microfluidic device for biological and chemical reaction and detection | |
| AU2004905872 | 2004-10-12 | ||
| AU2005901714 | 2005-04-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101031500A true CN101031500A (en) | 2007-09-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200580032736 Pending CN101031500A (en) | 2004-09-28 | 2005-09-02 | Micro-fluid device |
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| Country | Link |
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| CN (1) | CN101031500A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101981792A (en) * | 2008-03-28 | 2011-02-23 | 皇家飞利浦电子股份有限公司 | Microfluidic device and method |
| CN101713772B (en) * | 2008-10-06 | 2014-04-09 | 索尼株式会社 | Sensor for detecting analyte |
| CN107614421A (en) * | 2015-01-30 | 2018-01-19 | 惠普发展公司，有限责任合伙企业 | Micro Fluid Transfer |
| CN112680343A (en) * | 2020-12-17 | 2021-04-20 | 西安交通大学 | Electric self-driven trapping device and sepsis diagnostic device |
| US11401495B2 (en) | 2020-12-10 | 2022-08-02 | Alit Biotech (Shanghai) Co., Ltd. | Power device of a micro channel for external circulation of a bioreactor |
| CN116717507A (en) * | 2023-06-09 | 2023-09-08 | 兰州理工大学 | Array type ferrofluid driving pump for electrically exciting axial conveying gas-liquid mixed medium |
-
2005
- 2005-09-02 CN CN 200580032736 patent/CN101031500A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101981792A (en) * | 2008-03-28 | 2011-02-23 | 皇家飞利浦电子股份有限公司 | Microfluidic device and method |
| CN101713772B (en) * | 2008-10-06 | 2014-04-09 | 索尼株式会社 | Sensor for detecting analyte |
| CN107614421A (en) * | 2015-01-30 | 2018-01-19 | 惠普发展公司，有限责任合伙企业 | Micro Fluid Transfer |
| US10589269B2 (en) | 2015-01-30 | 2020-03-17 | Hewlett-Packard Development Company, L.P. | Microfluidic transport |
| CN107614421B (en) * | 2015-01-30 | 2021-04-30 | 惠普发展公司，有限责任合伙企业 | Microfluidic transport |
| US11401495B2 (en) | 2020-12-10 | 2022-08-02 | Alit Biotech (Shanghai) Co., Ltd. | Power device of a micro channel for external circulation of a bioreactor |
| CN112680343A (en) * | 2020-12-17 | 2021-04-20 | 西安交通大学 | Electric self-driven trapping device and sepsis diagnostic device |
| CN116717507A (en) * | 2023-06-09 | 2023-09-08 | 兰州理工大学 | Array type ferrofluid driving pump for electrically exciting axial conveying gas-liquid mixed medium |
| CN116717507B (en) * | 2023-06-09 | 2024-06-11 | 兰州理工大学 | Array type ferrofluid driving pump for electrically exciting axial conveying gas-liquid mixed medium |
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