ATE486127T1 - Wiederherstellung der aktivität von 5- enolpyruvylshikimat-3-phosphat-synthase durch fragmentkomplementierung - Google Patents

Wiederherstellung der aktivität von 5- enolpyruvylshikimat-3-phosphat-synthase durch fragmentkomplementierung

Info

Publication number
ATE486127T1
ATE486127T1 AT05819606T AT05819606T ATE486127T1 AT E486127 T1 ATE486127 T1 AT E486127T1 AT 05819606 T AT05819606 T AT 05819606T AT 05819606 T AT05819606 T AT 05819606T AT E486127 T1 ATE486127 T1 AT E486127T1
Authority
AT
Austria
Prior art keywords
present
epsps
phosphate synthase
enolpyruvylshikimat
restoration
Prior art date
Application number
AT05819606T
Other languages
English (en)
Inventor
Yiping Wang
Yicheng Sun
Yan Li
Original Assignee
Univ Beijing
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Beijing filed Critical Univ Beijing
Application granted granted Critical
Publication of ATE486127T1 publication Critical patent/ATE486127T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT05819606T 2004-12-16 2005-12-16 Wiederherstellung der aktivität von 5- enolpyruvylshikimat-3-phosphat-synthase durch fragmentkomplementierung ATE486127T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN200410102289.4A CN1789412B (zh) 2004-12-16 2004-12-16 利用片段互补技术重建5-烯醇丙酮酰莽草酸-3-磷酸合成酶活性
PCT/CN2005/002219 WO2006063533A1 (fr) 2004-12-16 2005-12-16 Reconstitution de l’activite de la 5-enolpyruvylshikimate-3-phosphate synthase par complementation de fragments

Publications (1)

Publication Number Publication Date
ATE486127T1 true ATE486127T1 (de) 2010-11-15

Family

ID=36587548

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05819606T ATE486127T1 (de) 2004-12-16 2005-12-16 Wiederherstellung der aktivität von 5- enolpyruvylshikimat-3-phosphat-synthase durch fragmentkomplementierung

Country Status (6)

Country Link
US (1) US8936925B2 (de)
EP (1) EP1840210B1 (de)
CN (1) CN1789412B (de)
AT (1) ATE486127T1 (de)
DE (1) DE602005024442D1 (de)
WO (1) WO2006063533A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2833613A1 (en) * 2011-04-29 2012-11-01 Keygene N.V. Glyphosate resistance enhancement
PL222067B1 (pl) * 2011-08-11 2016-06-30 Inst Biotechnologii I Antybiotyków Kaseta ekspresyjna, zastosowanie kasety ekspresyjnej, wektor ekspresyjny, komórka prokariotycznego gospodarza zawierająca wektor ekspresyjny, szczep bakteryjny oraz sposób wytwarzania polipeptydu
RU2626535C2 (ru) * 2012-02-01 2017-07-28 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Синтетические происходящие из brassica транзитные пептиды хлоропластов
CN104630245A (zh) * 2013-11-08 2015-05-20 北京大学 5-烯醇式丙酮酰-莽草酸-3-磷酸合酶的环式重排变体和互补片段对
CN106630493A (zh) * 2015-07-26 2017-05-10 李娜 一种去除含镉污水污染物的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2117991U (zh) 1992-03-20 1992-10-07 兰溪市金笔厂 一种弹簧复合书写笔
CN1268572A (zh) * 1999-03-31 2000-10-04 中国农业科学院生物技术研究中心 一种5－烯醇丙酮酰－莽草酸－3－磷酸合成酶基因的合成方法
JP2003505012A (ja) * 1999-05-24 2003-02-12 ニュー・イングランド・バイオラブズ・インコーポレイティッド 活性なタンパク質産生物を発現し得る分断した非伝達性遺伝子を産生させるための方法
DE10254167A1 (de) 2002-11-20 2004-06-09 Icon Genetics Ag Verfahren zur Kontrolle von zellulären Prozessen in Pflanzen
DE10254166A1 (de) 2002-11-20 2004-06-03 Icon Genetics Ag Verfahren zur Kontrolle eines zellulären Prozesses in Pflanzen

Also Published As

Publication number Publication date
DE602005024442D1 (de) 2010-12-09
WO2006063533A1 (fr) 2006-06-22
CN1789412A (zh) 2006-06-21
EP1840210A4 (de) 2008-11-05
EP1840210A1 (de) 2007-10-03
US8936925B2 (en) 2015-01-20
CN1789412B (zh) 2011-04-20
US20090155879A1 (en) 2009-06-18
EP1840210B1 (de) 2010-10-27

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