ATE422557T1 - Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen - Google Patents

Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen

Info

Publication number
ATE422557T1
ATE422557T1 AT01981538T AT01981538T ATE422557T1 AT E422557 T1 ATE422557 T1 AT E422557T1 AT 01981538 T AT01981538 T AT 01981538T AT 01981538 T AT01981538 T AT 01981538T AT E422557 T1 ATE422557 T1 AT E422557T1
Authority
AT
Austria
Prior art keywords
standard bases
methods
detection systems
solid supports
standard
Prior art date
Application number
AT01981538T
Other languages
German (de)
English (en)
Inventor
Jennifer Grenier
David Marshall
James Prudent
Craig Richmond
Eric Roesch
Christopher Scherrer
Christopher Sherrill
Jerod L Ptacin
Original Assignee
Eragen Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/861,292 external-priority patent/US7422850B2/en
Application filed by Eragen Biosciences Inc filed Critical Eragen Biosciences Inc
Application granted granted Critical
Publication of ATE422557T1 publication Critical patent/ATE422557T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Silicon Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT01981538T 2000-10-14 2001-10-15 Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen ATE422557T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US24039700P 2000-10-14 2000-10-14
US28283101P 2001-04-10 2001-04-10
US09/861,292 US7422850B2 (en) 2000-05-19 2001-05-18 Materials and methods for detection of nucleic acids
US29325901P 2001-05-22 2001-05-22

Publications (1)

Publication Number Publication Date
ATE422557T1 true ATE422557T1 (de) 2009-02-15

Family

ID=27500014

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01981538T ATE422557T1 (de) 2000-10-14 2001-10-15 Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen

Country Status (8)

Country Link
US (6) US6977161B2 (OSRAM)
EP (1) EP1358352B1 (OSRAM)
JP (1) JP4499987B2 (OSRAM)
AT (1) ATE422557T1 (OSRAM)
AU (2) AU2002213175B2 (OSRAM)
CA (1) CA2425747C (OSRAM)
DE (1) DE60137649D1 (OSRAM)
WO (1) WO2002033126A2 (OSRAM)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7125401A (en) 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
EP1358352B1 (en) * 2000-10-14 2009-02-11 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
US20030194705A1 (en) * 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding
WO2004065550A2 (en) * 2003-01-17 2004-08-05 Eragen Biosciences, Inc. Nucleic acid amplification using non-standard bases
CA2521127C (en) 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
US7282333B2 (en) * 2003-10-20 2007-10-16 Promega Corporation Methods and compositions for nucleic acid analysis
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
WO2006101913A2 (en) * 2005-03-18 2006-09-28 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of neiserria
EP2489746B1 (en) 2005-06-07 2016-02-03 Luminex Corporation Methods for detection and typing of nucleic acids
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
US7794936B1 (en) * 2006-09-29 2010-09-14 Steven Albert Benner Using thymidine analogs to improve replication in an expanded genetic alphabet
US20080153097A1 (en) * 2006-11-15 2008-06-26 Eragen Biosciences, Inc. Methods and kits for detecting jak2 nucleic acid
US9725713B1 (en) * 2007-01-22 2017-08-08 Steven A Benner In vitro selection with expanded genetic alphabets
WO2008134374A1 (en) * 2007-04-27 2008-11-06 Eragen Biosciences, Inc. Materials and methods for detection of hepatitis c virus
US20100086908A1 (en) * 2007-05-17 2010-04-08 Prudent James R Methods for the detection of respiratory viruses
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
EP2133434A1 (en) 2008-06-12 2009-12-16 Institut National de la Santé et de la Recherche Médicale (INSERM) Method for the detection of target nucleic acid
US10865431B1 (en) * 2008-06-17 2020-12-15 Steven A Benner Polymerase incorporation of non-standard nucleotides
CN102066574A (zh) * 2008-06-17 2011-05-18 史蒂文·埃尔伯特·奔纳 利用非天然核苷酸扩增dna的过程
US9637783B1 (en) * 2008-06-17 2017-05-02 Steven Benner Amplification of oligonucleotides containing non-standard nucleotides by polymerase chain reactions
EP2432796B1 (en) * 2009-05-21 2016-08-17 Siemens Healthcare Diagnostics Inc. Universal tags with non-natural nucleobases
JPWO2011043385A1 (ja) 2009-10-06 2013-03-04 独立行政法人理化学研究所 特異な塩基対を形成する人工塩基対
US8440406B2 (en) 2009-10-23 2013-05-14 Luminex Corporation Amplification primers with non-standard bases for increased reaction specificity
AU2012304520B2 (en) 2011-09-06 2016-06-16 Gen-Probe Incorporated Circularized templates for sequencing
US10767208B2 (en) 2011-09-06 2020-09-08 Gen-Probe Incorporated Closed nucleic acid structures
US10443085B2 (en) * 2012-06-20 2019-10-15 Toray Industries, Inc. Method for detecting nucleic acid and nucleic acid detection kit
WO2014205083A1 (en) 2013-06-19 2014-12-24 Luminex Corporation Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres
US9657332B2 (en) 2013-08-09 2017-05-23 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9670088B2 (en) 2014-05-20 2017-06-06 Corning Incorporated Scratch resistant glass and method of making
US20150361481A1 (en) 2014-06-13 2015-12-17 Life Technologies Corporation Multiplex nucleic acid amplification
WO2016025452A1 (en) 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays
AU2016257426B2 (en) 2015-05-01 2020-01-16 Gen-Probe Incorporated Multiplex invasive cleavage assays
WO2017015177A1 (en) 2015-07-17 2017-01-26 Luminex Corporation Methods and compositions for catalytic assays
EP3463388B1 (en) 2016-05-24 2024-09-11 Emory University Particles with rna cleaving nucleobase polymers and uses for managing inflammatory disorders

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
GB8902689D0 (en) 1989-02-07 1989-03-30 Ici Plc Assay method
US5856092A (en) 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
NZ235032A (en) 1989-08-31 1993-04-28 Dow Chemical Co Constrained geometry complexes of titanium, zirconium or hafnium comprising a substituted cyclopentadiene ligand; use as olefin polymerisation catalyst component
GB8920097D0 (en) 1989-09-06 1989-10-18 Ici Plc Amplification processes
US6007984A (en) 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5965364A (en) 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE4129653A1 (de) 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1994021820A1 (en) * 1993-03-17 1994-09-29 City Of Hope Nucleic acid sequences having single stranded ends
US6077668A (en) 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5654138A (en) 1993-05-14 1997-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Von hippel-lindau (VHL) disease gene and corresponding cDNA and methods for detecting carriers of the VHL disease gene
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5589329A (en) * 1993-11-15 1996-12-31 Ambion, Inc. Methods and compositions for detecting base pair mismatches
AU694187B2 (en) 1994-02-07 1998-07-16 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis TM of single nucleotide polymorphisms and its use in genetic analysis
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5866337A (en) * 1995-03-24 1999-02-02 The Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5980861A (en) * 1996-03-12 1999-11-09 University Of Massachusetts Chelator compositions and methods of synthesis thereof
AU2320597A (en) 1996-03-19 1997-10-10 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US5780233A (en) * 1996-06-06 1998-07-14 Wisconsin Alumni Research Foundation Artificial mismatch hybridization
US6124092A (en) * 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US5853993A (en) 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
JP4185185B2 (ja) 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖dnaを利用したdnaの検出方法
US5928869A (en) 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
CA2306907A1 (en) 1997-10-28 1999-05-06 The Regents Of The University Of California Dna base mismatch detection using flow cytometry
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
AU3996299A (en) 1998-05-14 1999-11-29 Luminex Corporation Diode laser based measurement apparatus
CA2335951C (en) 1998-06-24 2013-07-30 Mark S. Chee Decoding of array sensors with microspheres
CA2357601A1 (en) * 1998-12-30 2000-07-06 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
JP2001204497A (ja) * 2000-01-20 2001-07-31 Japan Science & Technology Corp 蛍光共鳴エネルギー移動を利用した、dnaハイブリダイゼーション法によるdna検出方法
US6783985B1 (en) * 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
AU7125401A (en) * 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
EP1358352B1 (en) 2000-10-14 2009-02-11 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
US20030194705A1 (en) 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding

Also Published As

Publication number Publication date
US7892796B2 (en) 2011-02-22
DE60137649D1 (de) 2009-03-26
EP1358352B1 (en) 2009-02-11
AU1317502A (en) 2002-04-29
AU2002213175B2 (en) 2007-09-13
US8518671B2 (en) 2013-08-27
US8217160B2 (en) 2012-07-10
US20140024550A1 (en) 2014-01-23
JP4499987B2 (ja) 2010-07-14
US20120295808A1 (en) 2012-11-22
US20070087361A1 (en) 2007-04-19
US20150132759A1 (en) 2015-05-14
EP1358352A2 (en) 2003-11-05
CA2425747C (en) 2012-01-24
US20040106108A1 (en) 2004-06-03
US6977161B2 (en) 2005-12-20
US20060078936A1 (en) 2006-04-13
US10240189B2 (en) 2019-03-26
US8969047B2 (en) 2015-03-03
WO2002033126A2 (en) 2002-04-25
JP2004531205A (ja) 2004-10-14
CA2425747A1 (en) 2002-04-25
WO2002033126A3 (en) 2003-08-28

Similar Documents

Publication Publication Date Title
ATE422557T1 (de) Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen
ATE220725T1 (de) Glycosylase verwendende detektion von bestimmten nukleinsäuresequenzen
ATE409238T1 (de) Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
EP1975251A3 (en) Real-time sequence determination
MXPA04003184A (es) Configuraciones ordenandas aleatoriamente y metodos de elaboracion y uso.
CA2310077A1 (en) De novo or "universal" sequencing array
ATE280246T1 (de) Polymorphismuserkennung mit hilfe cluster-analyse
DE50312579D1 (de) Vorrichtung zur Detektion von Targetmolekülen
ATE315666T1 (de) Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren
IL153848A0 (en) Microarray method of genotyping multiple samples at multiple loci
EP1816216A3 (de) Oligomer-Array mit PNA-und/oder Oligomeren auf einer Oberfläche
KR970028548A (ko) 핵산의 형광 편광도 검출방법
EP1484416A3 (en) Method of identifying and quantifying specific fungi and bacteria
PT938502E (pt) Metodo selectivo por acilacao
MY141856A (en) Method for gene identification signature (gis) analysis
ATE334390T1 (de) Adressierbares modulares erkennungssystem, seine herstellung und verwendung
NO20005428D0 (no) Varmelabil uracil-DNA-glykosylase samt anvendelse derav
IL149791A0 (en) Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample
ATE386821T1 (de) Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
BR0311768A (pt) Identificação de oligonucleotìdeos para captura, detecção e quantificação de ácido nucléico do virus da hepatite a
DE50008148D1 (de) Verfahren zur charakterisierung von nukleinsäurefragmenten
ATE310823T1 (de) Expressionssystem zur produktion von proteinen in pilzen
EP1118659A4 (en) EXPRESSION SYSTEMS FOR THE EXPRESSION OF FUNCTIONAL NUCLEIC ACIDS
ATE356885T1 (de) Spezifische multiplex-analyse von nukleinsäuren
MXPA03000992A (es) Marcador para detectar polimorfismo en genomas vegetales con el uso de un elemento transponible y metodo para construir el mismo.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties