ATE403719T1 - Konditionelles selex - Google Patents

Konditionelles selex

Info

Publication number
ATE403719T1
ATE403719T1 AT00947548T AT00947548T ATE403719T1 AT E403719 T1 ATE403719 T1 AT E403719T1 AT 00947548 T AT00947548 T AT 00947548T AT 00947548 T AT00947548 T AT 00947548T AT E403719 T1 ATE403719 T1 AT E403719T1
Authority
AT
Austria
Prior art keywords
selex
conditional
nucleic acid
signal
target molecule
Prior art date
Application number
AT00947548T
Other languages
English (en)
Inventor
Jonathan Smith
Larry Gold
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somalogic Inc filed Critical Somalogic Inc
Application granted granted Critical
Publication of ATE403719T1 publication Critical patent/ATE403719T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Glass Compositions (AREA)
  • Prostheses (AREA)
  • Polyesters Or Polycarbonates (AREA)
AT00947548T 1999-07-29 2000-07-19 Konditionelles selex ATE403719T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/364,541 US6506887B1 (en) 1999-07-29 1999-07-29 Conditional-selex

Publications (1)

Publication Number Publication Date
ATE403719T1 true ATE403719T1 (de) 2008-08-15

Family

ID=23434946

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00947548T ATE403719T1 (de) 1999-07-29 2000-07-19 Konditionelles selex

Country Status (9)

Country Link
US (2) US6506887B1 (de)
EP (1) EP1203006B1 (de)
JP (1) JP4565787B2 (de)
AT (1) ATE403719T1 (de)
AU (1) AU6113200A (de)
CA (1) CA2380395C (de)
DE (1) DE60039765D1 (de)
DK (1) DK1203006T3 (de)
WO (1) WO2001009158A1 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1543158B1 (de) * 2002-07-25 2011-07-13 Archemix Corp. Regulierte aptamer-therapeutika
US9303262B2 (en) * 2002-09-17 2016-04-05 Archemix Llc Methods for identifying aptamer regulators
GB0226374D0 (en) * 2002-11-12 2002-12-18 Isis Innovation Ligands
WO2005019430A2 (en) * 2003-08-22 2005-03-03 Research Development Foundation In vitro selection of aptamer beacons
WO2005051174A2 (en) * 2003-11-21 2005-06-09 The Trustees Of Columbia University In The City Of New York Nucleic acid aptamer-based compositions and methods
JP5543052B2 (ja) * 2003-11-22 2014-07-09 株式会社テクノメディカ アプタマーを用いた標的分子の検出方法
CA2545006C (en) * 2003-12-12 2013-09-17 Saint Louis University Biosensors for detecting macromolecules and other analytes
US7207206B2 (en) 2004-02-19 2007-04-24 Ut-Battelle, Llc Chemically-functionalized microcantilevers for detection of chemical, biological and explosive material
US9492400B2 (en) 2004-11-04 2016-11-15 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
CA2611198C (en) * 2005-06-10 2015-04-28 Saint Louis University Methods for the selection of aptamers
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
US9267937B2 (en) * 2005-12-15 2016-02-23 Massachusetts Institute Of Technology System for screening particles
WO2008105773A2 (en) * 2006-03-31 2008-09-04 Massachusetts Institute Of Technology System for targeted delivery of therapeutic agents
JP5630998B2 (ja) 2006-05-15 2014-11-26 マサチューセッツ インスティテュート オブ テクノロジー 機能的粒子のためのポリマー
US9381477B2 (en) 2006-06-23 2016-07-05 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
WO2008019142A2 (en) * 2006-08-04 2008-02-14 Massachusetts Institute Of Technology Oligonucleotide systems for targeted intracellular delivery
US9725713B1 (en) 2007-01-22 2017-08-08 Steven A Benner In vitro selection with expanded genetic alphabets
US8586303B1 (en) 2007-01-22 2013-11-19 Steven Albert Benner In vitro selection with expanded genetic alphabets
US9217129B2 (en) * 2007-02-09 2015-12-22 Massachusetts Institute Of Technology Oscillating cell culture bioreactor
WO2008124634A1 (en) 2007-04-04 2008-10-16 Massachusetts Institute Of Technology Polymer-encapsulated reverse micelles
WO2008124639A2 (en) 2007-04-04 2008-10-16 Massachusetts Institute Of Technology Poly (amino acid) targeting moieties
MX2010003642A (es) 2007-10-12 2010-08-09 Massachusetts Inst Technology Nanotecnologia de vacuna.
US8591905B2 (en) 2008-10-12 2013-11-26 The Brigham And Women's Hospital, Inc. Nicotine immunonanotherapeutics
US8343498B2 (en) * 2008-10-12 2013-01-01 Massachusetts Institute Of Technology Adjuvant incorporation in immunonanotherapeutics
US8277812B2 (en) * 2008-10-12 2012-10-02 Massachusetts Institute Of Technology Immunonanotherapeutics that provide IgG humoral response without T-cell antigen
US8343497B2 (en) 2008-10-12 2013-01-01 The Brigham And Women's Hospital, Inc. Targeting of antigen presenting cells with immunonanotherapeutics
CA2744003C (en) 2008-11-21 2016-03-15 Mediomics Llc Biosensor for detecting multiple epitopes on a target
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
EP2534266B1 (de) 2010-02-12 2016-07-06 Saint Louis University Molekulare biosensoren zur signalverstärkung
CN112587671A (zh) 2012-07-18 2021-04-02 博笛生物科技有限公司 癌症的靶向免疫治疗
BR112015009138A2 (pt) 2012-10-23 2020-10-20 Caris Life Sciences Switzerland Holdings, S.A.R.L. métodos para caracterizar um câncer
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
AU2013361323B2 (en) 2012-12-19 2018-09-06 Caris Science, Inc. Compositions and methods for aptamer screening
ES2916873T3 (es) 2014-01-10 2022-07-06 Birdie Biopharmaceuticals Inc Compuestos y composiciones para inmunoterapia
CN105440135A (zh) 2014-09-01 2016-03-30 博笛生物科技有限公司 用于治疗肿瘤的抗-pd-l1结合物
CN105233291A (zh) 2014-07-09 2016-01-13 博笛生物科技有限公司 用于治疗癌症的联合治疗组合物和联合治疗方法
JP6760919B2 (ja) 2014-07-09 2020-09-23 バーディー バイオファーマシューティカルズ インコーポレイテッド 腫瘍を治療するための抗ｐｄ−ｌ１組み合わせ
US10274484B2 (en) 2014-09-12 2019-04-30 Mediomics Llc Molecular biosensors with a modular design
CN107613980A (zh) 2015-05-31 2018-01-19 源生公司 用于免疫疗法的组合组合物
DK3344805T3 (da) * 2015-09-04 2022-03-07 Neoventures Biotechnology Inc Fremgangsmåde til udvælgelse af aptamerer til ubundne mål
EP3525782B1 (de) 2016-10-14 2023-12-06 CNS Biosciences, Inc. Behandlung von neuropathischen schmerzen bei personen mit rückenmarksverletzungen
EP3332812A1 (de) 2016-12-07 2018-06-13 Rheinische Friedrich-Wilhelms-Universität Bonn Nukleinsäurebasierte anordnung und verwendung der anordnung in der krebstherapie
WO2022073965A1 (en) 2020-10-07 2022-04-14 Centre National De La Recherche Scientifique (Cnrs) Process for selection of aptamers, riboswitches and desoxyriboswitches

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2183661B (en) 1985-03-30 1989-06-28 Marc Ballivet Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
US5641629A (en) * 1990-06-11 1997-06-24 Nexstar Pharmacueticals Inc Spectroscopically detectable nucleic acid ligands
US5683867A (en) * 1990-06-11 1997-11-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: blended SELEX
JP2763958B2 (ja) * 1990-06-11 1998-06-11 ネクスター ファーマスーティカルズ，インコーポレイテッド 核酸リガンド
US5789163A (en) * 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
CA2104698A1 (en) 1991-02-21 1992-08-22 John J. Toole Aptamers specific for biomolecules and methods of making
US5565322A (en) * 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
WO1994013791A1 (en) 1992-12-04 1994-06-23 Innovir Laboratories, Inc. Regulatable nucleic acid therapeutic and methods of use thereof
US6214546B1 (en) 1995-02-27 2001-04-10 Intelligene Ltd. Detection of biomolecules
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
CN1232509A (zh) 1996-08-26 1999-10-20 音坦里吉有限公司 催化性核酸及其医学用途
AU724627B2 (en) 1996-12-19 2000-09-28 Yale University Bioreactive allosteric polynucleotides
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
JP4153068B2 (ja) * 1997-12-24 2008-09-17 浜松ホトニクス株式会社 蛍光免疫測定方法
IL126731A0 (en) 1998-10-23 1999-08-17 Intelligene Ltd A method of detection
AU772881B2 (en) 1998-11-03 2004-05-13 Yale University Multidomain polynucleotide molecular sensors
IL130606A0 (en) 1999-06-23 2000-06-01 Intelligene Ltd An array of nucleic acid sequence
IL132710A0 (en) 1999-11-02 2001-03-19 Intelligene Ltd Catalytic nucleic acid sequences
KR20020089339A (ko) 2000-02-03 2002-11-29 리서치 디벨럽먼트 파운데이션 분자상 인식을 판별 시그날로 변환시키는 시그날링 압타머
EP1257639A2 (de) 2000-02-08 2002-11-20 Ribozyme Pharmaceuticals, Inc. Nukleozyme mit endonuklease aktivität
EP1290154A2 (de) 2000-03-01 2003-03-12 Amgen Inc. Identifizierung und verwendung von effektoren und allosterischen molekülen zur änderung der genexpression
JP2003525631A (ja) 2000-03-06 2003-09-02 リボザイム・ファーマシューティカルズ・インコーポレーテッド 核酸センサー分子

Also Published As

Publication number Publication date
JP4565787B2 (ja) 2010-10-20
EP1203006B1 (de) 2008-08-06
WO2001009158A1 (en) 2001-02-08
AU6113200A (en) 2001-02-19
DE60039765D1 (de) 2008-09-18
US20030087301A1 (en) 2003-05-08
EP1203006A1 (de) 2002-05-08
DK1203006T3 (da) 2008-12-15
CA2380395A1 (en) 2001-02-08
US6706482B2 (en) 2004-03-16
EP1203006A4 (de) 2005-01-12
US6506887B1 (en) 2003-01-14
CA2380395C (en) 2010-10-19
JP2003506670A (ja) 2003-02-18

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