ATE349555T1 - Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase - Google Patents

Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase

Info

Publication number
ATE349555T1
ATE349555T1 AT03716589T AT03716589T ATE349555T1 AT E349555 T1 ATE349555 T1 AT E349555T1 AT 03716589 T AT03716589 T AT 03716589T AT 03716589 T AT03716589 T AT 03716589T AT E349555 T1 ATE349555 T1 AT E349555T1
Authority
AT
Austria
Prior art keywords
genomic dna
sequences
methods
present
methylated
Prior art date
Application number
AT03716589T
Other languages
English (en)
Inventor
Cathy E Lofton-Day
John K Day
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Application granted granted Critical
Publication of ATE349555T1 publication Critical patent/ATE349555T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT03716589T 2002-03-15 2003-03-14 Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase ATE349555T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36468902P 2002-03-15 2002-03-15

Publications (1)

Publication Number Publication Date
ATE349555T1 true ATE349555T1 (de) 2007-01-15

Family

ID=28041950

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03716589T ATE349555T1 (de) 2002-03-15 2003-03-14 Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase

Country Status (6)

Country Link
US (1) US7285394B2 (de)
EP (1) EP1487999B1 (de)
AT (1) ATE349555T1 (de)
AU (1) AU2003220291A1 (de)
DE (1) DE60310697D1 (de)
WO (1) WO2003078593A2 (de)

Families Citing this family (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
JP4551216B2 (ja) * 2002-05-17 2010-09-22 ニューゲン テクノロジーズ, インコーポレイテッド 核酸の断片化、標識および固定化の方法
EP2339025B1 (de) * 2002-06-26 2013-10-09 Cold Spring Harbor Laboratory Verfahren zur Bestimmung des Methylierungsprofils
JP4786904B2 (ja) 2002-11-27 2011-10-05 セクエノム,インコーポレイティド 配列変化検出及び発見用の断片化をベースとする方法及びシステム
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
JP4917891B2 (ja) 2003-10-21 2012-04-18 オリオン ゲノミクス エルエルシー 差次的酵素的断片化の方法
AU2004311882A1 (en) * 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7459274B2 (en) * 2004-03-02 2008-12-02 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
CA2561381C (en) 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
DE102005023055A1 (de) * 2005-05-13 2006-11-16 Epigenomics Ag Verfahren zur Herstellung einer Mischung von Fragmenten einer Polynukleinsäure
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US8679741B2 (en) * 2006-05-31 2014-03-25 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
EP2038429B1 (de) * 2006-06-30 2013-08-21 Nugen Technologies, Inc. Verfahren zur fragmentierung und etikettierung von nukleinsäuren
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US20100086913A1 (en) * 2007-07-30 2010-04-08 Roche Molecular Systems, Inc. Methods of Identification Using Methylation of CPG
EP2195452B1 (de) 2007-08-29 2012-03-14 Sequenom, Inc. Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US8486634B2 (en) 2007-11-06 2013-07-16 Ambergen, Inc. Amplifying bisulfite-treated template
US20090264298A1 (en) * 2007-11-06 2009-10-22 Ambergen, Inc. Methods for enriching subpopulations
US20090286286A1 (en) * 2007-11-06 2009-11-19 Ambergen , Inc. Methods for controlling amplification
US8932879B2 (en) * 2007-11-06 2015-01-13 Ambergen, Inc. Methods and compounds for phototransfer
US20090270278A1 (en) * 2007-11-06 2009-10-29 Ambergen, Inc. Methods and compounds for making arrays
US8481263B2 (en) * 2007-11-06 2013-07-09 Ambergen Bead-ligand-nascent protein complexes
US20100075374A1 (en) * 2007-11-08 2010-03-25 Ambergen, Inc. Methods for capturing nascent proteins
US20090137024A1 (en) * 2007-11-28 2009-05-28 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
US7888127B2 (en) 2008-01-15 2011-02-15 Sequenom, Inc. Methods for reducing adduct formation for mass spectrometry analysis
US8852864B2 (en) * 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
EP4450642A2 (de) 2008-01-17 2024-10-23 Sequenom, Inc. Einzelmolekülnukleinsäurensequenzanalyseverfahren und zusammensetzungen
US8709726B2 (en) 2008-03-11 2014-04-29 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
EP3584324A1 (de) 2008-11-24 2019-12-25 Sequenom, Inc. Produkte und verfahren zur nukleinsäuresequenzierung
EP3514244B1 (de) 2009-04-03 2021-07-07 Sequenom, Inc. Verfahren für die herstellung von nukleinsäuren
EP3660165B1 (de) 2009-12-22 2023-01-04 Sequenom, Inc. Verfahren und kits zur identifizierung von aneuploidie
DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
CA2834218C (en) 2011-04-29 2021-02-16 Sequenom, Inc. Quantification of a minority nucleic acid species using inhibitory oligonucleotides
WO2012177792A2 (en) 2011-06-24 2012-12-27 Sequenom, Inc. Methods and processes for non-invasive assessment of a genetic variation
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2850785C (en) 2011-10-06 2022-12-13 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2852949A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
JP6431769B2 (ja) 2012-01-20 2018-11-28 セクエノム, インコーポレイテッド 実験条件を要因として含める診断プロセス
EP2807292B1 (de) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und erzeugung einer hocheffizienten bibliothek
ES2930180T3 (es) 2012-03-02 2022-12-07 Sequenom Inc Métodos para enriquecer ácido nucleico canceroso a partir de una muestra biológica
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2833923A4 (de) 2012-04-02 2016-02-24 Moderna Therapeutics Inc Modifizierte polynukleotide zur herstellung von proteinen
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
GB2518078B (en) 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20140004105A1 (en) 2012-06-29 2014-01-02 Sequenom, Inc. Age-related macular degeneration diagnostics
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
JP2015521862A (ja) 2012-07-13 2015-08-03 セクエノム, インコーポレイテッド 非侵襲性の出生前診断に有用な母体サンプル由来の胎児核酸のメチル化に基づく富化のためのプロセスおよび組成物
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130189684A1 (en) 2013-03-12 2013-07-25 Sequenom, Inc. Quantification of cell-specific nucleic acid markers
US9305756B2 (en) 2013-03-13 2016-04-05 Agena Bioscience, Inc. Preparation enhancements and methods of use for MALDI mass spectrometry
EP2971100A1 (de) 2013-03-13 2016-01-20 Sequenom, Inc. Primer zur analyse der dna-methylierung
EP4279610A3 (de) 2013-03-15 2024-01-03 ModernaTX, Inc. Ribonukleinsäurereinigung
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
EP2983804A4 (de) 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ionenaustauschreinigung von mrna
EP2971033B8 (de) 2013-03-15 2019-07-10 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
LT2981921T (lt) 2013-04-03 2023-02-27 Sequenom, Inc. Neinvazinio genetinių variacijų vertinimo būdai ir procesai
KR102540202B1 (ko) 2013-05-24 2023-06-02 시쿼넘, 인코포레이티드 유전적 변이의 비침습 평가를 위한 방법 및 프로세스
KR102299305B1 (ko) 2013-06-21 2021-09-06 시쿼넘, 인코포레이티드 유전적 변이의 비침습 평가를 위한 방법 및 프로세스
JP7019233B2 (ja) 2013-07-11 2022-02-15 モデルナティエックス インコーポレイテッド CRISPR関連タンパク質をコードする合成ポリヌクレオチドおよび合成sgRNAを含む組成物ならびに使用方法
EP3052511A4 (de) 2013-10-02 2017-05-31 Moderna Therapeutics, Inc. Polynukleotidmoleküle und verwendungen davon
ES2968644T3 (es) 2013-10-04 2024-05-13 Sequenom Inc Métodos y procedimientos para la evaluación no invasiva de variaciones genéticas
CN111863131A (zh) 2013-10-07 2020-10-30 塞昆纳姆股份有限公司 用于非侵入性评估染色体改变的方法和过程
EP3068883B1 (de) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur identifizierung einer duplikatsequenzierungslesung
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015138774A1 (en) 2014-03-13 2015-09-17 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2015196128A2 (en) 2014-06-19 2015-12-23 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
US20170136132A1 (en) * 2014-06-19 2017-05-18 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
US10407683B2 (en) 2014-07-16 2019-09-10 Modernatx, Inc. Circular polynucleotides
WO2016019042A1 (en) 2014-07-30 2016-02-04 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR101654004B1 (ko) * 2014-10-30 2016-09-05 서울대학교산학협력단 애기장대 ap 엔도뉴클레아제를 이용한 dna 메틸화 부위의 형광 표지 방법
EP3307755A4 (de) * 2015-05-12 2018-12-26 Wake Forest University Health Sciences Identifizierung genetischer modifikationen
AU2016324463B2 (en) 2015-09-17 2022-10-27 Modernatx, Inc. Polynucleotides containing a stabilizing tail region
US11434486B2 (en) 2015-09-17 2022-09-06 Modernatx, Inc. Polynucleotides containing a morpholino linker
WO2018022890A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Genetic copy number alteration classifications
US11694768B2 (en) 2017-01-24 2023-07-04 Sequenom, Inc. Methods and processes for assessment of genetic variations
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
WO2023244983A1 (en) * 2022-06-13 2023-12-21 Freenome Holdings, Inc. Sequence process validation methods and compositions
WO2024006783A2 (en) * 2022-06-30 2024-01-04 Illumina, Inc. Methylation detection with a non-natural/unnatural base
WO2024149841A1 (en) * 2023-01-13 2024-07-18 F. Hoffmann-La Roche Ag Detection of modified nucleobases in dna samples

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998017684A2 (en) 1996-10-25 1998-04-30 Advanced Research & Technology Institute Dna sequences encoding fusions of dna repair proteins and uses thereof
US20040137456A1 (en) * 2002-04-04 2004-07-15 Hiroki Yokota Method for identifying and characterizing individual dna molecules

Also Published As

Publication number Publication date
US20030180779A1 (en) 2003-09-25
DE60310697D1 (de) 2007-02-08
AU2003220291A8 (en) 2003-09-29
EP1487999A4 (de) 2005-08-17
US7285394B2 (en) 2007-10-23
EP1487999A2 (de) 2004-12-22
AU2003220291A1 (en) 2003-09-29
WO2003078593A2 (en) 2003-09-25
WO2003078593A3 (en) 2004-07-29
EP1487999B1 (de) 2006-12-27

Similar Documents

Publication Publication Date Title
ATE349555T1 (de) Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase
WO2003064701A3 (en) Method for the analysis of cytosine methylation patterns
DK0954608T3 (da) Methyleringsspecifik påvisning
BR0107574A (pt) Métodos e kits para identificar evento de elite gat-zm1 em amostras biológicas
AU2002311897A1 (en) Nucleic acid detection in pooled samples
AU2003291481A1 (en) Methods for identifying dna copy number changes
WO2005019477A3 (en) Methods and compositions for differentiating tissues or cell types using epigenetic markers
AU4372199A (en) Electrochemical based assay processes, instrument and labels
EP1373567A4 (de) "nachweis von sequenzen, die reich an methyliertem cpg sind, zur diagnose maligner zellen"
ATE464397T1 (de) Cpg-amplicon und array-protokoll
AU2001268449A1 (en) Toxicity typing using liver stem cells
AU2001285219A1 (en) Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
AU2002252297A1 (en) Methods and tools for nucleic acid sequence analysis selection and generation
AU1728001A (en) Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample
WO2001096604A3 (en) Assay for genetic polymorphisms using scattered light detectable labels
WO2005012548A3 (en) Self-hybridizing multiple target nucleic acid probes and methods of use
WO2004044164A3 (en) Method for identifying risk of melanoma and treatments thereof
AU2002303234A1 (en) Methods and apparatus for discovering, identifying and comparing biological activity mechanisms
ES2175095T3 (es) Marcadores de adn respecto al tamaño de una camada de cerdos.
WO2004062487A3 (en) Methods of detecting gene expression in normal and cancerous cells
AU2003256215A1 (en) Method and probe for identifying bacterial virulence modifying agents, agents thus identified, and their use
AU2159801A (en) Test for micro-organisms
AU2003233219A1 (en) Ssh based methods for identifying and isolating unique nucleic acid sequences
AU2003268634A1 (en) Culture having phenol oxidase-like activity
AU2002219230A1 (en) Method for the determination of at least one functional polymorphism in the nucleotide sequence of a preselected candidate gene and its applications

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties