ATE343137T1 - Verfahren zur bestimmung von polypeptiden - Google Patents

Verfahren zur bestimmung von polypeptiden

Info

Publication number
ATE343137T1
ATE343137T1 AT02735602T AT02735602T ATE343137T1 AT E343137 T1 ATE343137 T1 AT E343137T1 AT 02735602 T AT02735602 T AT 02735602T AT 02735602 T AT02735602 T AT 02735602T AT E343137 T1 ATE343137 T1 AT E343137T1
Authority
AT
Austria
Prior art keywords
polypeptide
capping
peptide fragments
free thiols
cleavage reagent
Prior art date
Application number
AT02735602T
Other languages
English (en)
Inventor
Christian Hamon
Andrew Thompson
Thomas Neumann
Robert Johnstone
Abdul Karim Abed Mohammed
Original Assignee
Electrophoretics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP01304975A external-priority patent/EP1267170A1/de
Priority claimed from EP01306842A external-priority patent/EP1265072A1/de
Application filed by Electrophoretics Ltd filed Critical Electrophoretics Ltd
Application granted granted Critical
Publication of ATE343137T1 publication Critical patent/ATE343137T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6821—Sequencing of polypeptides involving C-terminal degradation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT02735602T 2001-06-07 2002-06-07 Verfahren zur bestimmung von polypeptiden ATE343137T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01304975A EP1267170A1 (de) 2001-06-07 2001-06-07 Verfahren zur Charakterisierung von Polypeptiden
EP01306842A EP1265072A1 (de) 2001-06-07 2001-08-10 Verfahren zur Charakterisierung von Polypeptiden
EP02250740 2002-02-04

Publications (1)

Publication Number Publication Date
ATE343137T1 true ATE343137T1 (de) 2006-11-15

Family

ID=27224353

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02735602T ATE343137T1 (de) 2001-06-07 2002-06-07 Verfahren zur bestimmung von polypeptiden

Country Status (9)

Country Link
US (1) US7163803B2 (de)
EP (1) EP1397686B1 (de)
JP (1) JP4163103B2 (de)
AT (1) ATE343137T1 (de)
CA (1) CA2449680A1 (de)
DE (1) DE60215498T2 (de)
NO (1) NO20035402D0 (de)
NZ (1) NZ529985A (de)
WO (1) WO2002099435A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1267170A1 (de) * 2001-06-07 2002-12-18 Xzillion GmbH & CO.KG Verfahren zur Charakterisierung von Polypeptiden
EP1492757A1 (de) 2002-04-08 2005-01-05 Glaxo Group Limited (2-((2-alkoxy)-phenyl)-cyclopent-1-enyl) aromatische karbo- and heterozyklische karbonsäure und karbonsäurederivaten
GB0225548D0 (en) 2002-11-01 2002-12-11 Glaxo Group Ltd Compounds
AU2004212641B2 (en) * 2003-02-19 2009-08-06 Merck Millipore Ltd. Composite materials comprising supported porous gels
GB0306756D0 (en) 2003-03-24 2003-04-30 Xzillion Gmbh & Co Kg Mass labels
GB0317123D0 (en) * 2003-07-22 2003-08-27 Xzillion Gmbh & Co Kg Characterising polypeptides
WO2005075993A1 (en) * 2004-02-10 2005-08-18 Korea Basic Science Institute Selective labeling agent for phosphoproteome analysis and phosphorylated site analysis
CA2558859C (en) 2004-04-08 2014-02-04 Mcmaster University Membrane stacks
US8133840B2 (en) * 2004-06-07 2012-03-13 Natrix Separations Inc. Stable composite material comprising supported porous gels
AU2005307808B2 (en) 2004-11-15 2011-03-10 University Of North Dakota A method for single oxygen atom incorporation into digested peptides using peptidases
GB0518585D0 (en) 2005-09-12 2005-10-19 Electrophoretics Ltd Mass labels
US8580534B2 (en) 2006-06-30 2013-11-12 The University Of North Dakota Method for incorporation of two oxygen atoms into digested peptides using peptidases
US8859734B2 (en) * 2006-09-29 2014-10-14 Intel Corporation Method for the selective enrichment and labeling of phosphorproteins
US9086411B2 (en) * 2007-12-27 2015-07-21 Duke University Resin assisted capture of cysteine-modified proteins/peptides and determination of presence and location of modification
WO2010002911A2 (en) 2008-06-30 2010-01-07 H. Lee Moffitt Cancer Center And Research Institute, Inc. Methods and materials for monitoring myeloma using quantitative mass spetrometry
US20100059443A1 (en) 2008-09-02 2010-03-11 Natrix Separations Inc. Chromatography Membranes, Devices Containing Them, and Methods of Use Thereof
CA2780095C (en) * 2009-11-13 2018-12-11 Merck Millipore Ltd. Hydrophobic interaction chromatography membranes, and methods of use thereof
EP3427815B1 (de) 2011-05-17 2023-12-06 Merck Millipore Ltd. Vorrichtung mit geschichteten rohrmembranen für chromatographie
CN105051270B (zh) * 2013-03-15 2019-05-07 代表亚利桑那大学的亚利桑那校董会 生物传感器微阵列构成和方法
US9146219B2 (en) * 2014-01-27 2015-09-29 National Medical Services, Inc. Sensitive method for measuring cis-diol containing compounds in plasma using 2D-LC-MS/MS
EP3924997B1 (de) * 2019-02-15 2025-02-26 Ohio State Innovation Foundation Vorrichtungen und verfahren zur oberflächeninduzierten dissoziation
CN113252814A (zh) * 2021-05-25 2021-08-13 上海应用技术大学 一种大豆蛋白水解物苦味肽的鉴定方法
WO2023003882A2 (en) * 2021-07-19 2023-01-26 University Of North Carolina At Wilmington Compounds having selective inactivation activity

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0669399B2 (ja) 1988-03-15 1994-09-07 三菱化成株式会社 カルボキシル末端ペプチドの分取方法
JPH0648017B2 (ja) * 1988-07-12 1994-06-22 株式会社大金製作所 ダンパーディスク
US5470703A (en) 1992-10-21 1995-11-28 Shimadzu Corporation Method for peptide C-terminal fragment sequence analysis and apparatus for collecting peptide fragment
JP3353278B2 (ja) 1992-12-24 2002-12-03 株式会社島津製作所 ペプチドｎ末端フラグメントの分取方法
JPH07165789A (ja) 1993-12-15 1995-06-27 Toray Res Center:Kk タンパク質のアミノ末端ペプチドの分離方法
US5750360A (en) * 1995-06-07 1998-05-12 Lxr Biotechnology Inc. Method for quantitatively measuring apoptosis
DE69817211T2 (de) * 1997-01-23 2004-06-03 Xzillion Gmbh & Co. Kg Charakterisierung von polypeptiden
GB9821393D0 (en) 1998-10-01 1998-11-25 Brax Genomics Ltd Protein profiling 2
AUPQ664300A0 (en) 2000-04-03 2000-05-04 Proteome Systems Ltd Macromolecule detection
US20060141632A1 (en) * 2000-07-25 2006-06-29 The Procter & Gamble Co. New methods and kits for sequencing polypeptides
EP1265072A1 (de) * 2001-06-07 2002-12-11 Xzillion GmbH & CO.KG Verfahren zur Charakterisierung von Polypeptiden
EP1267170A1 (de) * 2001-06-07 2002-12-18 Xzillion GmbH & CO.KG Verfahren zur Charakterisierung von Polypeptiden

Also Published As

Publication number Publication date
WO2002099435A1 (en) 2002-12-12
US7163803B2 (en) 2007-01-16
CA2449680A1 (en) 2002-12-12
DE60215498D1 (de) 2006-11-30
EP1397686A1 (de) 2004-03-17
US20050095661A1 (en) 2005-05-05
EP1397686B1 (de) 2006-10-18
DE60215498T2 (de) 2007-04-26
NO20035402D0 (no) 2003-12-05
NZ529985A (en) 2006-02-24
JP4163103B2 (ja) 2008-10-08
JP2004537716A (ja) 2004-12-16

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