ATE332978T1 - Fluoreszenzverfahren zum messen von enzymaktivität unter verwendung von polyionen - Google Patents

Fluoreszenzverfahren zum messen von enzymaktivität unter verwendung von polyionen

Info

Publication number
ATE332978T1
ATE332978T1 AT04001161T AT04001161T ATE332978T1 AT E332978 T1 ATE332978 T1 AT E332978T1 AT 04001161 T AT04001161 T AT 04001161T AT 04001161 T AT04001161 T AT 04001161T AT E332978 T1 ATE332978 T1 AT E332978T1
Authority
AT
Austria
Prior art keywords
reagent
polyions
measuring
mixture
fluorescence method
Prior art date
Application number
AT04001161T
Other languages
English (en)
Inventor
Theo T Nikiforov
Original Assignee
Caliper Life Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/316,447 external-priority patent/US6287774B1/en
Application filed by Caliper Life Sciences Inc filed Critical Caliper Life Sciences Inc
Application granted granted Critical
Publication of ATE332978T1 publication Critical patent/ATE332978T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT04001161T 1999-05-21 2000-05-11 Fluoreszenzverfahren zum messen von enzymaktivität unter verwendung von polyionen ATE332978T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/316,447 US6287774B1 (en) 1999-05-21 1999-05-21 Assay methods and system
US13956299P 1999-06-16 1999-06-16
US15636699P 1999-09-28 1999-09-28

Publications (1)

Publication Number Publication Date
ATE332978T1 true ATE332978T1 (de) 2006-08-15

Family

ID=27385362

Family Applications (2)

Application Number Title Priority Date Filing Date
AT00935963T ATE278190T1 (de) 1999-05-21 2000-05-11 Fluoreszenzpolarizationsbestimmungen unter verwendung von polyionen
AT04001161T ATE332978T1 (de) 1999-05-21 2000-05-11 Fluoreszenzverfahren zum messen von enzymaktivität unter verwendung von polyionen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT00935963T ATE278190T1 (de) 1999-05-21 2000-05-11 Fluoreszenzpolarizationsbestimmungen unter verwendung von polyionen

Country Status (10)

Country Link
EP (1) EP1183536B1 (de)
JP (1) JP4472879B2 (de)
CN (1) CN1130564C (de)
AT (2) ATE278190T1 (de)
AU (1) AU762155B2 (de)
CA (1) CA2373747A1 (de)
DE (2) DE60014365T2 (de)
IL (1) IL146375A0 (de)
MX (1) MXPA01011831A (de)
NZ (1) NZ515384A (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013128330A1 (en) * 2012-02-28 2013-09-06 Koninklijke Philips N.V. Device for energy-based skin treatment
CN104048922A (zh) * 2014-06-26 2014-09-17 北京理工大学 一种荧光光谱偏振度和偏振角的测量方法
CN113091808A (zh) * 2021-03-29 2021-07-09 江苏利宏科技发展有限公司 一种具有综合信息管理系统的化工工控仪表及其系统

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
EP0938586A4 (de) * 1996-10-28 2002-01-30 Panvera Corp Messung der kinaseaktivität mittels fluoreszentpolarisation

Also Published As

Publication number Publication date
CA2373747A1 (en) 2000-11-30
DE60014365T2 (de) 2005-10-13
EP1183536B1 (de) 2004-09-29
NZ515384A (en) 2003-08-29
AU5134300A (en) 2000-12-12
DE60014365D1 (de) 2004-11-04
IL146375A0 (en) 2002-07-25
EP1183536A1 (de) 2002-03-06
CN1355884A (zh) 2002-06-26
CN1130564C (zh) 2003-12-10
DE60029363D1 (de) 2006-08-24
ATE278190T1 (de) 2004-10-15
JP2003502620A (ja) 2003-01-21
JP4472879B2 (ja) 2010-06-02
MXPA01011831A (es) 2003-09-04
DE60029363T2 (de) 2007-08-02
AU762155B2 (en) 2003-06-19

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