CY1110515T1 - Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος - Google Patents
Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεοςInfo
- Publication number
- CY1110515T1 CY1110515T1 CY20091101052T CY091101052T CY1110515T1 CY 1110515 T1 CY1110515 T1 CY 1110515T1 CY 20091101052 T CY20091101052 T CY 20091101052T CY 091101052 T CY091101052 T CY 091101052T CY 1110515 T1 CY1110515 T1 CY 1110515T1
- Authority
- CY
- Cyprus
- Prior art keywords
- compound
- protein
- library
- fusion
- union
- Prior art date
Links
- 230000004927 fusion Effects 0.000 title abstract 4
- 230000003993 interaction Effects 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 6
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Στο παρόν ανακοινώνεται μέθοδος εντοπισμού αλληλεπίδρασης ένωσης-πρωτεΐνης που περιλαμβάνει: (α) παροχή βιβλιοθήκης ενώσεων στην οποία κάθε μέλος της βιβλιοθήκης ενώσεων είναι ακινητοποιημένο σε στερεό μέσο υποστήριξης, (β) επαφή κάθε μέλους της ακινητοποιημένης βιβλιοθήκης ενώσεων σε μονό θάλαμο αντίδρασης, με κάθε μέλος της βιβλιοθήκης συντήξεων πρωτεΐνης-νουκλεϊκού οξέος, υπό συνθήκες που επιτρέπουν το σχηματισμό συμπλεγμάτων ένωσης-σύντηξης, (γ) απομόνωση των ακινητοποιημένων συμπλεγμάτων ένωσης-σύντηξης, και (δ) εντοπισμό συμπλέγματος ένωσης-σύντηξης ως ένδειξη ότι η πρωτεΐνη της σύντηξης αλληλεπιδρά με την ένωση. Σε προτιμώμενες εφαρμογές, η πρωτεΐνη προσδιορίζεται με ανάγνωση του τμήματος νουκλεϊκού οξέος της σύντηξης, και η ένωση προσδιορίζεται με ανάγνωση ανιχνεύσιμης ετικέτας που είναι δεσμευμένη είτε στην ένωση είτε στο στερεό μέσο υποστήριξης.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9682098P | 1998-08-17 | 1998-08-17 | |
EP99948042A EP1105360B1 (en) | 1998-08-17 | 1999-08-16 | Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1110515T1 true CY1110515T1 (el) | 2015-04-29 |
Family
ID=22259237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20091101052T CY1110515T1 (el) | 1998-08-17 | 2009-10-13 | Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος |
Country Status (12)
Country | Link |
---|---|
US (1) | US6602685B1 (el) |
EP (1) | EP1105360B1 (el) |
JP (1) | JP4278872B2 (el) |
AT (1) | ATE437844T1 (el) |
AU (1) | AU757955B2 (el) |
CA (1) | CA2337490C (el) |
CY (1) | CY1110515T1 (el) |
DE (1) | DE69941193D1 (el) |
DK (1) | DK1105360T3 (el) |
ES (1) | ES2329205T3 (el) |
PT (1) | PT1105360E (el) |
WO (1) | WO2000009464A1 (el) |
Families Citing this family (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6517781B1 (en) * | 1997-06-02 | 2003-02-11 | Aurora Biosciences Corporation | Low fluorescence assay platforms and related methods for drug discovery |
WO2001016352A1 (en) * | 1999-08-27 | 2001-03-08 | Phylos, Inc. | Methods for encoding and sorting in vitro translated proteins |
US7022479B2 (en) | 2000-01-24 | 2006-04-04 | Compound Therapeutics, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
US6489116B2 (en) | 2000-01-24 | 2002-12-03 | Phylos, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
GB2360282A (en) * | 2000-03-17 | 2001-09-19 | Bioinvent Int Ab | Making and using micro-arrays of biological materials |
US6962781B1 (en) | 2000-05-19 | 2005-11-08 | Proteonova, Inc. | In vitro evolution of nucleic acids and encoded polypeptide |
US20040229271A1 (en) | 2000-05-19 | 2004-11-18 | Williams Richard B. | Compositions and methods for the identification and selection of nucleic acids and polypeptides |
US7410761B2 (en) * | 2000-05-19 | 2008-08-12 | Proteonova, Inc. | System for rapid identification and selection of nucleic acids and polypeptides, and method thereof |
US6980674B2 (en) | 2000-09-01 | 2005-12-27 | Large Scale Proteomics Corp. | Reference database |
US6539102B1 (en) | 2000-09-01 | 2003-03-25 | Large Scale Proteomics | Reference database |
WO2003029490A1 (en) * | 2001-10-01 | 2003-04-10 | Yingjian Wang | Methods and arrays for detecting biological molecules |
WO2003045975A2 (en) * | 2001-11-27 | 2003-06-05 | Compound Therapeutics, Inc. | Solid-phase immobilization of proteins and peptides |
US20060014145A1 (en) * | 2002-04-26 | 2006-01-19 | Miller Brian S | Methods and articles for high throughput analysis of biomolecular interactions |
US20070191402A1 (en) * | 2002-05-17 | 2007-08-16 | Kyowa Hakko Kogyo Co., Ltd. | Method of searching substrate having antidiabetic activity |
WO2004010106A2 (en) | 2002-07-24 | 2004-01-29 | Ptc Therapeutics, Inc. | METHODS FOR IDENTIFYING SMALL MOLEDULES THAT MODULATE PREMATURE TRANSLATION TERMINATION AND NONSENSE MEDIATED mRNA DECAY |
US20040142379A1 (en) * | 2003-01-16 | 2004-07-22 | Carlsberg Research Laboratory | Affinity fishing for ligands and proteins receptors |
CN1906307B (zh) * | 2003-11-20 | 2011-08-24 | 圣诺菲·帕斯图尔公司 | 纯化百日咳毒素的方法及其中有用的肽 |
AU2006295515A1 (en) * | 2005-09-27 | 2007-04-05 | Polybatics Limited | Polymer particles and uses thereof |
ES2547554T3 (es) | 2005-11-16 | 2015-10-07 | Ambrx, Inc. | Métodos y composiciones que comprenden aminoácidos no naturales |
US7749957B2 (en) | 2006-04-06 | 2010-07-06 | E.I. Du Pont De Nemours And Company | Clay-binding peptides and methods of use |
WO2009009186A2 (en) | 2007-04-13 | 2009-01-15 | The Regents Of The University Of California | Receptors useful for gas phase chemical sensing |
US7829311B2 (en) | 2007-07-25 | 2010-11-09 | E.I. Du Pont De Nemours And Company | Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate |
US7951559B2 (en) * | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
US7678883B2 (en) | 2007-07-25 | 2010-03-16 | E.I. Du Pont De Nemours And Company | Solubility tags for the expression and purification of bioactive peptides |
US7794963B2 (en) | 2007-11-02 | 2010-09-14 | E.I. Du Pont De Nemours And Company | Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins |
US8883146B2 (en) | 2007-11-30 | 2014-11-11 | Abbvie Inc. | Protein formulations and methods of making same |
SG10201604258YA (en) | 2007-11-30 | 2016-07-28 | Abbvie Biotechnology Ltd | Anti-tnf antibody formulations |
JP5924937B2 (ja) | 2008-07-25 | 2016-05-25 | エックス−ボディ インコーポレイテッド | タンパク質スクリーニング法 |
US20100158846A1 (en) * | 2008-12-18 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Hair-binding peptides |
US8287845B2 (en) * | 2008-12-18 | 2012-10-16 | E I Du Pont De Nemours And Company | Hair-binding peptides |
US20100158822A1 (en) | 2008-12-18 | 2010-06-24 | E .I. Du Pont De Nemours And Company | Peptides that bind to silica-coated particles |
US20100158837A1 (en) | 2008-12-18 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Iron oxide-binding peptides |
BRPI1014677A2 (pt) | 2009-03-30 | 2016-04-12 | Du Pont | sistemas baseados em peptídeos para distribuição de agentes cosméticos |
WO2010114638A1 (en) | 2009-03-30 | 2010-10-07 | E. I. Du Pont De Nemours And Company | Peptide-based tooth whitening reagents |
IN2012DN06588A (el) | 2010-02-10 | 2015-10-23 | Novartis Ag | |
DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
ES2926988T3 (es) | 2011-03-15 | 2022-10-31 | X Body Inc | Métodos de cribado de anticuerpos |
MX2013011000A (es) * | 2011-03-24 | 2014-03-27 | Opko Pharmaceuticals Llc | Descubrimiento de biomarcador en fluido biologico complejo usando genotecas basadas en microesferao particula y kits de diagnostico y terapeuticos. |
CN117026386A (zh) | 2011-04-28 | 2023-11-10 | 小利兰斯坦福大学托管委员会 | 与样品相关的多核苷酸的鉴定 |
TWI591074B (zh) | 2011-12-05 | 2017-07-11 | X 染色體有限公司 | PDGF受體β結合多肽 |
WO2014145458A1 (en) * | 2013-03-15 | 2014-09-18 | Arizona Board Of Regents On Behalf Of Arizona State University | Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling |
SG10201806428YA (en) | 2013-06-28 | 2018-08-30 | X Body Inc | Target antigen discovery, phenotypic screens and use thereof for identification of target cell specific target epitopes |
US10519436B2 (en) | 2013-09-23 | 2019-12-31 | X-Body, Inc. | Methods and compositions for generation of binding agents against cell surface antigens |
US10421958B2 (en) | 2014-02-05 | 2019-09-24 | Molecular Templates, Inc. | Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity |
Family Cites Families (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6361943B1 (en) | 1996-10-17 | 2002-03-26 | Mitsubishi Chemical Corporation | Molecule that homologizes genotype and phenotype and utilization thereof |
US4537861A (en) * | 1983-02-03 | 1985-08-27 | Elings Virgil B | Apparatus and method for homogeneous immunoassay |
US4587044A (en) | 1983-09-01 | 1986-05-06 | The Johns Hopkins University | Linkage of proteins to nucleic acids |
DE3590766C2 (el) | 1985-03-30 | 1991-01-10 | Marc Genf/Geneve Ch Ballivet | |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
US5424186A (en) * | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
DK0494955T3 (da) | 1989-10-05 | 1998-10-26 | Optein Inc | Cellefri syntese og isolering af hidtil ukendte gener og polypeptider |
US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
US5723286A (en) | 1990-06-20 | 1998-03-03 | Affymax Technologies N.V. | Peptide library and screening systems |
US5650489A (en) | 1990-07-02 | 1997-07-22 | The Arizona Board Of Regents | Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof |
US5843701A (en) * | 1990-08-02 | 1998-12-01 | Nexstar Pharmaceticals, Inc. | Systematic polypeptide evolution by reverse translation |
WO1992002536A1 (en) | 1990-08-02 | 1992-02-20 | The Regents Of The University Of Colorado | Systematic polypeptide evolution by reverse translation |
US5264563A (en) | 1990-08-24 | 1993-11-23 | Ixsys Inc. | Process for synthesizing oligonucleotides with random codons |
DE4112440C1 (el) | 1991-04-16 | 1992-10-22 | Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De | |
US5795747A (en) | 1991-04-16 | 1998-08-18 | Evotec Biosystems Gmbh | Process for manufacturing new biopolymers |
WO1993003172A1 (en) | 1991-08-01 | 1993-02-18 | University Research Corporation | Systematic polypeptide evolution by reverse translation |
US5639603A (en) | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
US5733731A (en) | 1991-10-16 | 1998-03-31 | Affymax Technologies N.V. | Peptide library and screening method |
US5270170A (en) | 1991-10-16 | 1993-12-14 | Affymax Technologies N.V. | Peptide library and screening method |
US5541061A (en) | 1992-04-29 | 1996-07-30 | Affymax Technologies N.V. | Methods for screening factorial chemical libraries |
US5565324A (en) | 1992-10-01 | 1996-10-15 | The Trustees Of Columbia University In The City Of New York | Complex combinatorial chemical libraries encoded with tags |
US6087186A (en) * | 1993-07-16 | 2000-07-11 | Irori | Methods and apparatus for synthesizing labeled combinatorial chemistry libraries |
US5635602A (en) | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
WO1995011922A1 (en) | 1993-10-29 | 1995-05-04 | Affymax Technologies N.V. | In vitro peptide and antibody display libraries |
US5561043A (en) | 1994-01-31 | 1996-10-01 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
DK0744958T3 (da) | 1994-01-31 | 2003-10-20 | Univ Boston | Polyklonale antistofbiblioteker |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
JPH10500951A (ja) | 1994-05-23 | 1998-01-27 | スミスクライン・ビーチャム・コーポレイション | コードされた組合せライブラリー |
US5627024A (en) | 1994-08-05 | 1997-05-06 | The Scripps Research Institute | Lambdoid bacteriophage vectors for expression and display of foreign proteins |
US5559000A (en) | 1995-01-18 | 1996-09-24 | The Scripps Research Institute | Encoded reaction cassette |
US5751629A (en) * | 1995-04-25 | 1998-05-12 | Irori | Remotely programmable matrices with memories |
US6100026A (en) * | 1995-04-25 | 2000-08-08 | Irori | Matrices with memories and uses thereof |
US5763263A (en) * | 1995-11-27 | 1998-06-09 | Dehlinger; Peter J. | Method and apparatus for producing position addressable combinatorial libraries |
US5942443A (en) * | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
US5872015A (en) * | 1996-05-10 | 1999-02-16 | Board Of Trustees Of The University Of Illinois | Molecular diversity screening method |
US5962645A (en) * | 1996-10-04 | 1999-10-05 | University Of Maryland | Antiproliferative factor from patients with interstitial cystitis |
PT971946E (pt) * | 1997-01-21 | 2006-11-30 | Gen Hospital Corp | Selecção de proteínas utilizando fusões arn-proteína |
GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
GB9712512D0 (en) | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US5985575A (en) | 1998-05-20 | 1999-11-16 | Wisconsin Alumni Research Foundation | Tethered function assay for protein function |
-
1999
- 1999-08-16 AU AU61296/99A patent/AU757955B2/en not_active Expired
- 1999-08-16 CA CA2337490A patent/CA2337490C/en not_active Expired - Lifetime
- 1999-08-16 AT AT99948042T patent/ATE437844T1/de active
- 1999-08-16 PT PT99948042T patent/PT1105360E/pt unknown
- 1999-08-16 DK DK99948042T patent/DK1105360T3/da active
- 1999-08-16 JP JP2000564919A patent/JP4278872B2/ja not_active Expired - Lifetime
- 1999-08-16 DE DE69941193T patent/DE69941193D1/de not_active Expired - Lifetime
- 1999-08-16 ES ES99948042T patent/ES2329205T3/es not_active Expired - Lifetime
- 1999-08-16 US US09/374,964 patent/US6602685B1/en not_active Expired - Lifetime
- 1999-08-16 EP EP99948042A patent/EP1105360B1/en not_active Expired - Lifetime
- 1999-08-16 WO PCT/US1999/018600 patent/WO2000009464A1/en active IP Right Grant
-
2009
- 2009-10-13 CY CY20091101052T patent/CY1110515T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
AU757955B2 (en) | 2003-03-13 |
AU6129699A (en) | 2000-03-06 |
EP1105360B1 (en) | 2009-07-29 |
JP2002522057A (ja) | 2002-07-23 |
JP4278872B2 (ja) | 2009-06-17 |
ES2329205T3 (es) | 2009-11-23 |
PT1105360E (pt) | 2009-09-30 |
US6602685B1 (en) | 2003-08-05 |
ATE437844T1 (de) | 2009-08-15 |
EP1105360A4 (en) | 2005-06-22 |
DK1105360T3 (da) | 2009-11-02 |
EP1105360A1 (en) | 2001-06-13 |
WO2000009464A1 (en) | 2000-02-24 |
CA2337490C (en) | 2012-01-03 |
CA2337490A1 (en) | 2000-02-24 |
WO2000009464A9 (en) | 2000-09-28 |
DE69941193D1 (de) | 2009-09-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CY1110515T1 (el) | Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος | |
Johannsson et al. | A fast highly sensitive colorimetric enzyme immunoassay system demonstrating benefits of enzyme amplification in clinical chemistry | |
EP0156641B1 (en) | Enzymic method of detecting analytes and novel substrates therefor | |
Choi‐Rhee et al. | Promiscuous protein biotinylation by Escherichia coli biotin protein ligase | |
Meyer et al. | Orthogonal protein decoration of DNA nanostructures | |
CY1109246T1 (el) | Μεθοδος ανιχνευσης συνδετη | |
US20080254494A1 (en) | Assays for measuring nucleic acid binding proteins and enzyme activities | |
DE60118527D1 (de) | Sensible und multiplexe diagnostische tests zur proteinanalyse | |
WO1999066324A3 (en) | A bioluminescence resonance energy transfer (bret) system and its use | |
DE60135912D1 (de) | Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation | |
GB0030727D0 (en) | Methods and kits for detecting kinase activity | |
WO2000015850A1 (en) | Assays for measuring nucleic acid binding proteins and enzyme activities | |
RU2004126254A (ru) | Анализ активности киназы actriib | |
Zhou et al. | Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification | |
Sheng et al. | A nanopore sensing assay resolves cascade reactions in a multienzyme system | |
Guo et al. | Thrombin-linked aptamer assay for detection of platelet derived growth factor BB on magnetic beads in a sandwich format | |
WO2011051709A1 (en) | A proximity ligation assay involving generation of catalytic activity | |
Li et al. | Targeted quantification of functional enzyme dynamics in environmental samples for microbially mediated biogeochemical processes | |
Visser et al. | Surface‐Plasmon‐Resonance‐Based Chemical Proteomics: Efficient Specific Extraction and Semiquantitative Identification of Cyclic Nucleotide‐Binding Proteins from Cellular Lysates by Using a Combination of Surface Plasmon Resonance, Sequential Elution and Liquid Chromatography–Tandem Mass Spectrometry | |
Lovrinovic et al. | Microtiter plate‐based screening for the optimization of DNA–protein conjugate synthesis by means of expressed protein ligation | |
Gramatikova et al. | Pyridoxal 5′-phosphate-dependent catalytic antibody | |
EP1117841A4 (en) | METHOD FOR DETECTING COMPOUNDS THAT INFLUENCE THE INTERACTION OF RNA AND RNA-BINDING PROTEINS | |
Fan et al. | Development of a capillary electrophoresis‐based assay of sirtuin enzymes | |
WO2002014542A3 (en) | Process for detecting serine/threonine kinase activity | |
Gramatikova et al. | Monoclonal Antibodies against Nα-(5′-Phosphopyridoxyl)-L-lysine: SCREENING AND SPECTRUM OF PYRIDOXAL 5′-PHOSPHATE-DEPENDENT ACTIVITIES TOWARD AMINO ACIDS |