EP0938586A4 - Messung der kinaseaktivität mittels fluoreszentpolarisation - Google Patents

Messung der kinaseaktivität mittels fluoreszentpolarisation

Info

Publication number
EP0938586A4
EP0938586A4 EP97952171A EP97952171A EP0938586A4 EP 0938586 A4 EP0938586 A4 EP 0938586A4 EP 97952171 A EP97952171 A EP 97952171A EP 97952171 A EP97952171 A EP 97952171A EP 0938586 A4 EP0938586 A4 EP 0938586A4
Authority
EP
European Patent Office
Prior art keywords
kinase activity
fluorescence polarization
activity measurement
measurement
polarization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97952171A
Other languages
English (en)
French (fr)
Other versions
EP0938586A1 (de
Inventor
Thomas J Burke
Randall E Bolger
Gregory Parker
Rebecca P Schall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Panvera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Panvera Corp filed Critical Panvera Corp
Publication of EP0938586A1 publication Critical patent/EP0938586A1/de
Publication of EP0938586A4 publication Critical patent/EP0938586A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP97952171A 1996-10-28 1997-10-28 Messung der kinaseaktivität mittels fluoreszentpolarisation Withdrawn EP0938586A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2983196P 1996-10-28 1996-10-28
US29831P 1996-10-28
PCT/US1997/019570 WO1998018956A1 (en) 1996-10-28 1997-10-28 Kinase activity measurement using fluorescence polarization

Publications (2)

Publication Number Publication Date
EP0938586A1 EP0938586A1 (de) 1999-09-01
EP0938586A4 true EP0938586A4 (de) 2002-01-30

Family

ID=21851124

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97952171A Withdrawn EP0938586A4 (de) 1996-10-28 1997-10-28 Messung der kinaseaktivität mittels fluoreszentpolarisation

Country Status (2)

Country Link
EP (1) EP0938586A4 (de)
WO (1) WO1998018956A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1036192B1 (de) 1997-12-05 2003-02-26 PHARMACIA & UPJOHN COMPANY Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase
US6656696B2 (en) 1999-02-26 2003-12-02 Cyclacel Compositions and methods for monitoring the phosphorylation of natural binding partners
US6287774B1 (en) 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
IL146375A0 (en) * 1999-05-21 2002-07-25 Caliper Techn Corp Fluorescence polarization assays involving polyions
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
EP1418239B1 (de) * 1999-05-21 2006-07-12 Caliper Life Sciences, Inc. Fluoreszenzverfahren zum Messen von Enzymaktivität unter Verwendung von Polyionen
EP1701163A1 (de) * 1999-05-21 2006-09-13 Caliper Life Sciences, Inc. Fluoreszenzpolarizationsbestimmungen unter Verwendung von Polyionen
GB9923208D0 (en) * 1999-10-01 1999-12-08 Cambridge Drug Discovery Ltd Assay
AU4067301A (en) * 2000-03-02 2001-09-12 Morphochem Ag Process for obtaining inhibitors/activators of an enzyme
GB0007915D0 (en) * 2000-03-31 2000-05-17 Univ Bristol Protein kinase assay
WO2001094614A2 (en) * 2000-06-07 2001-12-13 Cyclacel Limited Methods for monitoring enzyme activity
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
US20090263821A1 (en) 2004-12-01 2009-10-22 Proteologics, Inc. Ubiquitin Ligase Assays And Related Reagents

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997039326A2 (en) * 1996-04-18 1997-10-23 Ariad Pharmaceuticals, Inc. In vitro fluorescence polarization assay
US6203994B1 (en) * 1997-12-05 2001-03-20 Pharmacia & Upjohn Company Fluorescence-based high throughput sereening assays for protein kinases and phosphatases

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252492A (en) * 1991-03-12 1993-10-12 University Of Utah Research Foundation Fluorescence assay for ligand or receptor utilizing size exclusion
CA2121842A1 (en) * 1991-11-12 1993-05-27 John W. Shultz Non-radioactive enzyme assay

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997039326A2 (en) * 1996-04-18 1997-10-23 Ariad Pharmaceuticals, Inc. In vitro fluorescence polarization assay
US6203994B1 (en) * 1997-12-05 2001-03-20 Pharmacia & Upjohn Company Fluorescence-based high throughput sereening assays for protein kinases and phosphatases

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHECOVICH W J ET AL: "Fluorescence polaritation - a new tool for cell and molecular biology", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 375, 18 May 1995 (1995-05-18), pages 254 - 256, XP002100426, ISSN: 0028-0836 *
DANDLIKER, WALTER B. ET AL: "Equilibrium and kinetic inhibition assays based upon fluorescence polarization", METHODS ENZYMOL. (1981), 74(IMMUNOCHEM. TECH., PT. C), 3-28, 1981, XP001029241 *
See also references of WO9818956A1 *
SEETHALA R, MENZEL R, SQUIBB BRISTOL-MYERS: "A Fluorescence Polarization Tyrosine Kinase Assay for High Thr oughput Screening", ANNUAL CONFERENCE OF THE SOCIETY FOR BIOMOLECULAR SCREENING, XX, XX, no. 3, 22 September 1997 (1997-09-22) - 25 September 1997 (1997-09-25), XX, pages 6 - 59, XP002968015 *
WRIGHT D E ET AL: "FLUOROMETRIC ASSAY FOR CYCLIC AMP DEPENDENT PROTEIN KINASE EC-2.7.1.37 AND PHOSPHO PROTEIN PHOSPHATASE EC-3.1.3.16 ACTIVITIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 78, no. 10, 1981, 1981, pages 6048 - 6050, XP001029505, ISSN: 0027-8424 *
ZEMLAN F P ET AL: "MONOCLONAL ANTIBODY PHF-9 RECOGNIZES PHOSPHORYLATED SERINE 404 OF TAU PROTEIN AND LABELS PAIRED HELICAL FILAMENTS", JOURNAL OF NEUROSCIENCE RESEARCH, WILEY-LISS, US, vol. 46, no. 1, 1 October 1996 (1996-10-01), pages 90 - 97, XP000646724, ISSN: 0360-4012 *
Z-Y ZHANG ET AL: "A Continuous Spectrophotometric and Fluorimetric Assay for Protein Tyrosine Phosphatase Using Phosphotyrosine-Containing Peptides", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 211, 1993, pages 7 - 15, XP002100427, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
WO1998018956A1 (en) 1998-05-07
EP0938586A1 (de) 1999-09-01

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