EP0938586A4 - Kinase activity measurement using fluorescence polarization - Google Patents
Kinase activity measurement using fluorescence polarizationInfo
- Publication number
- EP0938586A4 EP0938586A4 EP97952171A EP97952171A EP0938586A4 EP 0938586 A4 EP0938586 A4 EP 0938586A4 EP 97952171 A EP97952171 A EP 97952171A EP 97952171 A EP97952171 A EP 97952171A EP 0938586 A4 EP0938586 A4 EP 0938586A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- kinase activity
- fluorescence polarization
- activity measurement
- measurement
- polarization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2983196P | 1996-10-28 | 1996-10-28 | |
US29831P | 1996-10-28 | ||
PCT/US1997/019570 WO1998018956A1 (en) | 1996-10-28 | 1997-10-28 | Kinase activity measurement using fluorescence polarization |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0938586A1 EP0938586A1 (en) | 1999-09-01 |
EP0938586A4 true EP0938586A4 (en) | 2002-01-30 |
Family
ID=21851124
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP97952171A Withdrawn EP0938586A4 (en) | 1996-10-28 | 1997-10-28 | Kinase activity measurement using fluorescence polarization |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP0938586A4 (en) |
WO (1) | WO1998018956A1 (en) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2192801T3 (en) | 1997-12-05 | 2003-10-16 | Upjohn Co | HIGH PRODUCTIVITY CRIBE TESTS OF PROTEIN KINASES AND PHOSPHATES, BASED ON FLUORESCENCE. |
US6656696B2 (en) | 1999-02-26 | 2003-12-02 | Cyclacel | Compositions and methods for monitoring the phosphorylation of natural binding partners |
US6472141B2 (en) * | 1999-05-21 | 2002-10-29 | Caliper Technologies Corp. | Kinase assays using polycations |
US6287774B1 (en) * | 1999-05-21 | 2001-09-11 | Caliper Technologies Corp. | Assay methods and system |
ATE332978T1 (en) * | 1999-05-21 | 2006-08-15 | Caliper Life Sciences Inc | FLUORESCENCE METHOD FOR MEASURING ENZYM ACTIVITY USING POLYIONS |
EP1418239B1 (en) * | 1999-05-21 | 2006-07-12 | Caliper Life Sciences, Inc. | Fluorescence assays for measuring enzyme activities involving polyions |
EP1701163A1 (en) * | 1999-05-21 | 2006-09-13 | Caliper Life Sciences, Inc. | Fluorescence polarization assays involving polyions |
GB9923208D0 (en) * | 1999-10-01 | 1999-12-08 | Cambridge Drug Discovery Ltd | Assay |
WO2001064939A2 (en) * | 2000-03-02 | 2001-09-07 | Morphochem Ag | Process for obtaining inhibitors/activators of an enzyme |
GB0007915D0 (en) * | 2000-03-31 | 2000-05-17 | Univ Bristol | Protein kinase assay |
EP1290215A2 (en) * | 2000-06-07 | 2003-03-12 | Cyclacel Limited | Methods for monitoring enzyme activity |
US6808874B2 (en) | 2000-06-13 | 2004-10-26 | Cyclacel Ltd. | Methods of monitoring enzyme activity |
EP1820024B1 (en) | 2004-12-01 | 2012-06-13 | Proteologics, Ltd | Ubiquitin ligase assays and related reagents |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997039326A2 (en) * | 1996-04-18 | 1997-10-23 | Ariad Pharmaceuticals, Inc. | In vitro fluorescence polarization assay |
US6203994B1 (en) * | 1997-12-05 | 2001-03-20 | Pharmacia & Upjohn Company | Fluorescence-based high throughput sereening assays for protein kinases and phosphatases |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5252492A (en) * | 1991-03-12 | 1993-10-12 | University Of Utah Research Foundation | Fluorescence assay for ligand or receptor utilizing size exclusion |
EP0646242A4 (en) * | 1991-11-12 | 1997-12-03 | Promega Corp | Non-radioactive enzyme assay. |
-
1997
- 1997-10-28 WO PCT/US1997/019570 patent/WO1998018956A1/en not_active Application Discontinuation
- 1997-10-28 EP EP97952171A patent/EP0938586A4/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997039326A2 (en) * | 1996-04-18 | 1997-10-23 | Ariad Pharmaceuticals, Inc. | In vitro fluorescence polarization assay |
US6203994B1 (en) * | 1997-12-05 | 2001-03-20 | Pharmacia & Upjohn Company | Fluorescence-based high throughput sereening assays for protein kinases and phosphatases |
Non-Patent Citations (7)
Title |
---|
CHECOVICH W J ET AL: "Fluorescence polaritation - a new tool for cell and molecular biology", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 375, 18 May 1995 (1995-05-18), pages 254 - 256, XP002100426, ISSN: 0028-0836 * |
DANDLIKER, WALTER B. ET AL: "Equilibrium and kinetic inhibition assays based upon fluorescence polarization", METHODS ENZYMOL. (1981), 74(IMMUNOCHEM. TECH., PT. C), 3-28, 1981, XP001029241 * |
See also references of WO9818956A1 * |
SEETHALA R, MENZEL R, SQUIBB BRISTOL-MYERS: "A Fluorescence Polarization Tyrosine Kinase Assay for High Thr oughput Screening", ANNUAL CONFERENCE OF THE SOCIETY FOR BIOMOLECULAR SCREENING, XX, XX, no. 3, 22 September 1997 (1997-09-22) - 25 September 1997 (1997-09-25), XX, pages 6 - 59, XP002968015 * |
WRIGHT D E ET AL: "FLUOROMETRIC ASSAY FOR CYCLIC AMP DEPENDENT PROTEIN KINASE EC-2.7.1.37 AND PHOSPHO PROTEIN PHOSPHATASE EC-3.1.3.16 ACTIVITIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 78, no. 10, 1981, 1981, pages 6048 - 6050, XP001029505, ISSN: 0027-8424 * |
ZEMLAN F P ET AL: "MONOCLONAL ANTIBODY PHF-9 RECOGNIZES PHOSPHORYLATED SERINE 404 OF TAU PROTEIN AND LABELS PAIRED HELICAL FILAMENTS", JOURNAL OF NEUROSCIENCE RESEARCH, WILEY-LISS, US, vol. 46, no. 1, 1 October 1996 (1996-10-01), pages 90 - 97, XP000646724, ISSN: 0360-4012 * |
Z-Y ZHANG ET AL: "A Continuous Spectrophotometric and Fluorimetric Assay for Protein Tyrosine Phosphatase Using Phosphotyrosine-Containing Peptides", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 211, 1993, pages 7 - 15, XP002100427, ISSN: 0003-2697 * |
Also Published As
Publication number | Publication date |
---|---|
EP0938586A1 (en) | 1999-09-01 |
WO1998018956A1 (en) | 1998-05-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 19990526 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB IE IT LI NL SE |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20011218 |
|
AK | Designated contracting states |
Kind code of ref document: A4 Designated state(s): AT BE CH DE DK ES FR GB IE IT LI NL SE |
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RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: INVITROGEN CORPORATION |
|
17Q | First examination report despatched |
Effective date: 20040113 |
|
18D | Application deemed to be withdrawn |
Effective date: 20040724 |
|
D18D | Application deemed to be withdrawn (deleted) | ||
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
R18D | Application deemed to be withdrawn (corrected) |
Effective date: 20060819 |