ATE321871T1 - Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten - Google Patents

Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten

Info

Publication number
ATE321871T1
ATE321871T1 AT96921541T AT96921541T ATE321871T1 AT E321871 T1 ATE321871 T1 AT E321871T1 AT 96921541 T AT96921541 T AT 96921541T AT 96921541 T AT96921541 T AT 96921541T AT E321871 T1 ATE321871 T1 AT E321871T1
Authority
AT
Austria
Prior art keywords
tta
tetracycline
expression
ttak
autoregulatory
Prior art date
Application number
AT96921541T
Other languages
English (en)
Inventor
David G Schatz
Original Assignee
Univ Yale
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Yale filed Critical Univ Yale
Application granted granted Critical
Publication of ATE321871T1 publication Critical patent/ATE321871T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
AT96921541T 1995-06-07 1996-06-07 Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten ATE321871T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/474,169 US5851796A (en) 1995-06-07 1995-06-07 Autoregulatory tetracycline-regulated system for inducible gene expression in eucaryotes

Publications (1)

Publication Number Publication Date
ATE321871T1 true ATE321871T1 (de) 2006-04-15

Family

ID=23882456

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96921541T ATE321871T1 (de) 1995-06-07 1996-06-07 Ein autoreguliertes tetracyclin-reguliertes system fur die induzierte genexpression in eukaryoten

Country Status (7)

Country Link
US (1) US5851796A (de)
EP (1) EP0832254B1 (de)
JP (1) JPH11507539A (de)
AT (1) ATE321871T1 (de)
AU (1) AU6274596A (de)
DE (1) DE69635992T2 (de)
WO (1) WO1996040946A1 (de)

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Also Published As

Publication number Publication date
EP0832254B1 (de) 2006-03-29
DE69635992T2 (de) 2007-02-01
DE69635992D1 (de) 2006-05-18
US5851796A (en) 1998-12-22
WO1996040946A1 (en) 1996-12-19
AU6274596A (en) 1996-12-30
EP0832254A1 (de) 1998-04-01
JPH11507539A (ja) 1999-07-06

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