ATE318894T1 - Intein-vermittelte cyclisierung von peptiden - Google Patents

Intein-vermittelte cyclisierung von peptiden

Info

Publication number
ATE318894T1
ATE318894T1 AT99966402T AT99966402T ATE318894T1 AT E318894 T1 ATE318894 T1 AT E318894T1 AT 99966402 T AT99966402 T AT 99966402T AT 99966402 T AT99966402 T AT 99966402T AT E318894 T1 ATE318894 T1 AT E318894T1
Authority
AT
Austria
Prior art keywords
intein
peptides
methods
target peptide
cyclic
Prior art date
Application number
AT99966402T
Other languages
English (en)
Inventor
Stephen J Benkovic
Charles P Scott
Ernesto V Abel-Santos
Original Assignee
Penn State Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Penn State Res Found filed Critical Penn State Res Found
Application granted granted Critical
Publication of ATE318894T1 publication Critical patent/ATE318894T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/50—Cyclic peptides containing at least one abnormal peptide link
    • C07K7/52—Cyclic peptides containing at least one abnormal peptide link with only normal peptide links in the ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/64—Cyclic peptides containing only normal peptide links
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99966402T 1998-12-18 1999-12-18 Intein-vermittelte cyclisierung von peptiden ATE318894T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11272398P 1998-12-18 1998-12-18
US15807799P 1999-10-07 1999-10-07

Publications (1)

Publication Number Publication Date
ATE318894T1 true ATE318894T1 (de) 2006-03-15

Family

ID=26810271

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99966402T ATE318894T1 (de) 1998-12-18 1999-12-18 Intein-vermittelte cyclisierung von peptiden

Country Status (11)

Country Link
US (2) US7354756B1 (de)
EP (1) EP1141250B1 (de)
JP (1) JP4544601B2 (de)
CN (1) CN1179040C (de)
AT (1) ATE318894T1 (de)
AU (1) AU781215B2 (de)
CA (1) CA2355934A1 (de)
DE (1) DE69930164T2 (de)
DK (1) DK1141250T3 (de)
ES (1) ES2260956T3 (de)
WO (1) WO2000036093A2 (de)

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US7566765B2 (en) 2000-03-06 2009-07-28 Rigel Pharmaceuticals, Inc. Heterocyclic compounds containing a nine-membered carbon-nitrogen ring
US7252952B2 (en) 2000-03-06 2007-08-07 Rigel Pharmaceuticals, Inc. In vivo production of cyclic peptides for inhibiting protein—protein interaction
US7378248B2 (en) 2000-03-06 2008-05-27 Rigel Pharmaceuticals, Inc. In vivo production of cyclic peptides for inhibiting protein-protein interaction
EP1263777A2 (de) 2000-03-06 2002-12-11 Rigel Pharmaceuticals, Inc. In vivo herstellung von zyklischen peptiden
EP1229330B1 (de) * 2000-07-26 2007-02-21 Japan Science and Technology Agency Sonde zum analysieren von protein-protein-wechselwirkungen und entsprechendes verfahren dafür
US7279304B2 (en) 2000-12-18 2007-10-09 President And Fellows Of Harvard College Methods for preparation of macrocyclic molecules and macrocyclic molecules prepared thereby
US20020177691A1 (en) * 2001-03-20 2002-11-28 Scott Charles P. Trans inteins for protein domain shuffling and biopolymerization
EP2395084B1 (de) 2002-01-08 2018-03-14 Agrivida, Inc. CIVPS- oder Intein-modifizierte Proteine exprimierende transgene Pflanzen und damit verbundenes Verfahren
US7250551B2 (en) 2002-07-24 2007-07-31 President And Fellows Of Harvard College Transgenic mice expressing inducible human p25
JP4287633B2 (ja) * 2002-09-18 2009-07-01 独立行政法人科学技術振興機構 オルガネラ局在タンパク質の解析方法と解析材料
FI20021726A0 (fi) * 2002-09-27 2002-09-27 Ctt Cancer Targeting Tech Oy Menetelmä peptidien tuottamiseksi
WO2004053460A2 (en) * 2002-12-11 2004-06-24 New England Biolabs, Inc. Carrier-ligand fusions and uses thereof
US7872096B2 (en) * 2004-05-24 2011-01-18 Rigel Pharmaceuticals, Inc. Methods for cyclizing synthetic polymers
US20100143972A1 (en) * 2006-12-14 2010-06-10 Horswill Alexander R Method of Making Cyclic Polypeptides with Inteins
CN101646771A (zh) * 2007-01-10 2010-02-10 萨斯喀彻温大学 环肽结构的稳定
CN101638642B (zh) * 2008-08-01 2012-02-22 中国农业科学院生物技术研究所 提高了热稳定性及蛋白酶抗性的环化植酸酶
WO2010135277A2 (en) * 2009-05-18 2010-11-25 Brown University Cyclic-glur6 analogs, methods of treatment and use
US8420387B2 (en) 2009-11-06 2013-04-16 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US10407742B2 (en) 2009-11-06 2019-09-10 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US9464333B2 (en) 2009-11-06 2016-10-11 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
CN107058285A (zh) * 2010-08-13 2017-08-18 米蒂生物系统有限公司 修饰肽的展示
US8986953B2 (en) 2011-01-20 2015-03-24 University Of Rochester Macrocyclic compounds with a hybrid peptidic/non-peptidic backbone and methods for their preparation
BR112013023003B1 (pt) 2011-03-07 2020-12-15 Agrivida, Inc Método para produção de açúcares solúveis a partir de material de planta genéticamente modificada e cassete de expressão
JP6177780B2 (ja) * 2011-09-28 2017-08-09 エラ、ビオテック、ソシエダッド、アノニマＥｒａ Ｂｉｏｔｅｃｈ， Ｓ．Ａ． スプリットインテインおよびその使用
EP3431497B1 (de) * 2012-06-27 2022-07-27 The Trustees of Princeton University Geteilte inteine, konjugate und verwendungen davon
WO2014055778A2 (en) 2012-10-03 2014-04-10 Agrivida, Inc. Multiprotein expression cassettes
DE102013016002A1 (de) * 2013-09-26 2015-03-26 Forschungszentrum Jülich GmbH Zyklische, Amyloid-Beta-bindende Peptide und deren Verwendung
US10995118B2 (en) 2013-09-26 2021-05-04 Forschungszentrum Juelich Gmbh Amyloid-beta-binding peptides and the use thereof for the treatment and diagnosis of alzheimer's disease
EP4122945A1 (de) 2013-12-23 2023-01-25 University of Rochester Verfahren und zusammensetzungen zur ribosomalen synthese von makrocyclischen peptiden
US20180118799A1 (en) 2015-04-13 2018-05-03 National Institute Of Advanced Industrial Science And Technology Cyclized cytokine and method for producing same
CN106749497B (zh) * 2017-02-06 2020-10-30 陈铭 套索多肽的化学全合成
GB201702938D0 (en) * 2017-02-23 2017-04-12 Univ Southampton Methods of generating and screening compartmentalised peptides libraries
CA3062843C (en) * 2017-04-26 2023-02-14 Hunan Zonsen Peplib Biotech Co., Ltd Peptide library constructing method
WO2018215831A2 (en) 2017-05-22 2018-11-29 National Hellenic Research Foundation Macrocyclic modulators of disease associated protein misfolding and aggregation
KR102000490B1 (ko) * 2018-01-12 2019-10-01 전남대학교산학협력단 가용성이 개선된 살모넬라균 편모 유래 플라젤린 단백질 발현 형질전환체, 그 제조방법 및 용도
GB201909491D0 (en) * 2019-07-01 2019-08-14 Governing Council Of The Univ Of Toronto Detection of protein to protein interactions
CN114667293A (zh) * 2019-09-24 2022-06-24 Mvrix株式会社 抗病毒活性增强的纳米穿孔器
CN115093470B (zh) * 2022-06-30 2023-03-24 广州市乾相生物科技有限公司 内含肽Mtu RecA突变体及其在生产谷胱甘肽GSH中的应用

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JP3662948B2 (ja) * 1992-12-09 2005-06-22 ニユー・イングランド・バイオレイブス・インコーポレイテツド 改質タンパク質及びその製造法
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US5498531A (en) * 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
CA2236948C (en) * 1995-06-28 2008-10-28 New England Biolabs, Inc. Modified proteins and methods of their production

Also Published As

Publication number Publication date
JP4544601B2 (ja) 2010-09-15
DK1141250T3 (da) 2006-07-10
CN1179040C (zh) 2004-12-08
DE69930164T2 (de) 2006-11-23
CA2355934A1 (en) 2000-06-22
DE69930164D1 (de) 2006-04-27
EP1141250B1 (de) 2006-03-01
ES2260956T3 (es) 2006-11-01
US20070207502A1 (en) 2007-09-06
WO2000036093A3 (en) 2000-10-19
WO2000036093A9 (en) 2001-08-16
WO2000036093A2 (en) 2000-06-22
EP1141250A2 (de) 2001-10-10
AU781215B2 (en) 2005-05-12
US7354756B1 (en) 2008-04-08
AU2194800A (en) 2000-07-03
JP2003534768A (ja) 2003-11-25
CN1354788A (zh) 2002-06-19
US7846710B2 (en) 2010-12-07

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