ATE311445T1 - Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen - Google Patents

Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen

Info

Publication number
ATE311445T1
ATE311445T1 AT01921457T AT01921457T ATE311445T1 AT E311445 T1 ATE311445 T1 AT E311445T1 AT 01921457 T AT01921457 T AT 01921457T AT 01921457 T AT01921457 T AT 01921457T AT E311445 T1 ATE311445 T1 AT E311445T1
Authority
AT
Austria
Prior art keywords
dna
organisms
size
sample
indirect
Prior art date
Application number
AT01921457T
Other languages
English (en)
Inventor
Renaud Nalin
Patrick Robe
Van Van Tran
Original Assignee
Libragen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Libragen filed Critical Libragen
Application granted granted Critical
Publication of ATE311445T1 publication Critical patent/ATE311445T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
AT01921457T 2000-04-26 2001-04-03 Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen ATE311445T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0005274A FR2808276B1 (fr) 2000-04-26 2000-04-26 Procede d'extraction indirecte de l'adn d'organismes non cultivables et adn susceptible d'etre obtenu par ledit procede
PCT/FR2001/000992 WO2001081357A2 (fr) 2000-04-26 2001-04-03 Procede d'extraction indirecte de l'adn d'organismes non cultivables et adn susceptible d'être obtenu par ledit procede

Publications (1)

Publication Number Publication Date
ATE311445T1 true ATE311445T1 (de) 2005-12-15

Family

ID=8849580

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01921457T ATE311445T1 (de) 2000-04-26 2001-04-03 Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen

Country Status (10)

Country Link
US (2) US6989249B2 (de)
EP (1) EP1278831B1 (de)
AT (1) ATE311445T1 (de)
AU (1) AU2001248447A1 (de)
CA (1) CA2407270C (de)
DE (1) DE60115415T2 (de)
DK (1) DK1278831T3 (de)
ES (1) ES2250376T3 (de)
FR (1) FR2808276B1 (de)
WO (1) WO2001081357A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005508495A (ja) 2001-09-06 2005-03-31 ジェノミック プロファイリング システムズ インコーポレイティッド 分子の迅速かつ高感度の検出法
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
EP1386966A1 (de) 2002-07-24 2004-02-04 Libragen Verfahren zur Expresssion von aus Umweltproben stammender DNA in Wirtszellen
DK1530633T3 (da) 2002-08-20 2010-09-27 B R A I N Biotechnology Res An Isolering og kloning af DNA fra ikke-dyrkede organismer
WO2004104179A2 (en) * 2003-05-16 2004-12-02 Wisconsin Alumni Research Foundation Method for isolating and cloning high molecular weight polynucleotide molecules from the environment
WO2007038478A2 (en) 2005-09-26 2007-04-05 Rapid Micro Biosystems, Inc Cassette containing growth medium
EP1842915A1 (de) 2006-04-04 2007-10-10 Libragen In vitro Methode zur Polynukleotide Sequenz Durchmischung durch die Fragmentierung und Ligierung von rekursiv zirkulären DNA Molekülen
EP2340301A4 (de) 2008-09-24 2012-05-09 Straus Holdings Inc Bildgebungsanalysegerät zum testen von analyten
WO2010064628A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 核酸含有試料の調製方法、試料調製用溶液、及び核酸の解析方法
WO2011113999A1 (en) * 2010-03-15 2011-09-22 University Of Oulu New polypeptides from endophytes of empetrum nigrum and their fragments, encoding them, their production and uses
CN103890163B (zh) 2011-06-19 2016-09-14 阿博根公司 用于样品采集的装置、溶液和方法
US9745546B2 (en) 2011-11-07 2017-08-29 Rapid Micro Biosystems, Inc. Cassette for sterility testing
CN104364388B (zh) 2012-04-16 2017-12-19 快速微型生物系统公司 细胞培养装置
CN106255753B (zh) 2014-03-07 2020-11-06 Dna吉诺特克股份有限公司 稳定化生物学样本中的核酸的组合物和方法
WO2016178207A1 (en) * 2015-05-04 2016-11-10 Ramot At Tel-Aviv University Ltd. Methods and kits for fragmenting dna
CN106754874A (zh) * 2016-12-06 2017-05-31 浙江指针基因科技有限公司 一种从人体粪便样本中提取dna的试剂盒及其使用方法
JP7347849B2 (ja) 2018-04-19 2023-09-20 ファースト ライト ダイアグノスティクス， インコーポレイテッド 標的の検出

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2010879A1 (en) * 1989-03-07 1990-09-07 Kathleen Potter Speciation of mycobacteria by pulsed-field gel electrophoresis of chromosomal dna fragments
EP0512181A1 (de) * 1991-05-07 1992-11-11 N.V. Innogenetics S.A. Verfahren zum in vitro Nachweis von Chromosomenanomalien, korrelierend mit der CMT1A-Krankheit
WO1994022904A1 (en) * 1993-04-07 1994-10-13 Gradipore Limited Improvements in protein separation
US5872001A (en) * 1994-04-20 1999-02-16 Uab Research Foundation Lanthionine antibiotic compositions and methods
US5824485A (en) 1995-04-24 1998-10-20 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
CA2232709C (en) 1995-09-22 2002-11-19 Terragen Diversity Inc. Method for isolating xylanase gene sequences from soil dna, compositions useful in such method and compositions obtained thereby
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US5965128A (en) * 1997-08-13 1999-10-12 University Of Georgia Research Foundation Inc. Control of enterohemorrhagic E. coli 0157:H7 in cattle by probiotic bacteria and specific strains of E. coli
EP0939118A1 (de) 1998-02-20 1999-09-01 Universiteit Maastricht Verfahren zur Isolierung von DNA und RNA aus Stuhlproben
MXPA00008559A (es) 1998-03-04 2005-06-06 Diversa Corp Produccion y uso de bibliotecas de adn normalizadas.
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant

Also Published As

Publication number Publication date
DE60115415D1 (de) 2006-01-05
US6989249B2 (en) 2006-01-24
FR2808276B1 (fr) 2004-04-02
ES2250376T3 (es) 2006-04-16
EP1278831A2 (de) 2003-01-29
EP1278831B1 (de) 2005-11-30
DK1278831T3 (da) 2005-12-27
AU2001248447A1 (en) 2001-11-07
US20060188967A1 (en) 2006-08-24
CA2407270A1 (fr) 2001-11-01
US20030049675A1 (en) 2003-03-13
WO2001081357A3 (fr) 2002-03-14
CA2407270C (fr) 2010-10-19
DE60115415T2 (de) 2006-06-14
FR2808276A1 (fr) 2001-11-02
WO2001081357A2 (fr) 2001-11-01

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