DE60115415D1 - Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen - Google Patents

Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen

Info

Publication number
DE60115415D1
DE60115415D1 DE60115415T DE60115415T DE60115415D1 DE 60115415 D1 DE60115415 D1 DE 60115415D1 DE 60115415 T DE60115415 T DE 60115415T DE 60115415 T DE60115415 T DE 60115415T DE 60115415 D1 DE60115415 D1 DE 60115415D1
Authority
DE
Germany
Prior art keywords
organisms
dna
cultivatable
micro
size
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60115415T
Other languages
English (en)
Other versions
DE60115415T2 (de
Inventor
Renaud Nalin
Patrick Robe
Van Van Tran
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Givaudan France SAS
Original Assignee
Libragen SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Libragen SA filed Critical Libragen SA
Application granted granted Critical
Publication of DE60115415D1 publication Critical patent/DE60115415D1/de
Publication of DE60115415T2 publication Critical patent/DE60115415T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
DE60115415T 2000-04-26 2001-04-03 Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen Expired - Lifetime DE60115415T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0005274A FR2808276B1 (fr) 2000-04-26 2000-04-26 Procede d'extraction indirecte de l'adn d'organismes non cultivables et adn susceptible d'etre obtenu par ledit procede
PCT/FR2001/000992 WO2001081357A2 (fr) 2000-04-26 2001-04-03 Procede d'extraction indirecte de l'adn d'organismes non cultivables et adn susceptible d'être obtenu par ledit procede

Publications (2)

Publication Number Publication Date
DE60115415D1 true DE60115415D1 (de) 2006-01-05
DE60115415T2 DE60115415T2 (de) 2006-06-14

Family

ID=8849580

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60115415T Expired - Lifetime DE60115415T2 (de) 2000-04-26 2001-04-03 Verfahren zur indirekten isolation von dns aus nicht-kultivierbaren mikroorganismen

Country Status (10)

Country Link
US (2) US6989249B2 (de)
EP (1) EP1278831B1 (de)
AT (1) ATE311445T1 (de)
AU (1) AU2001248447A1 (de)
CA (1) CA2407270C (de)
DE (1) DE60115415T2 (de)
DK (1) DK1278831T3 (de)
ES (1) ES2250376T3 (de)
FR (1) FR2808276B1 (de)
WO (1) WO2001081357A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE470859T1 (de) 2001-09-06 2010-06-15 Straus Holdings Inc Schneller und empfindlicher nachweis von molekülen
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
EP1386966A1 (de) 2002-07-24 2004-02-04 Libragen Verfahren zur Expresssion von aus Umweltproben stammender DNA in Wirtszellen
WO2004018673A2 (en) * 2002-08-20 2004-03-04 B.R.A.I.N. Biotechnology Research And Information Network Ag Isolation and cloning of dna from uncultivated organisms
CA2526985A1 (en) * 2003-05-16 2004-12-02 Wisconsin Alumni Research Foundation Method for isolating and cloning high molecular weight polynucleotide molecules from the environment
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
EP1842915A1 (de) 2006-04-04 2007-10-10 Libragen In vitro Methode zur Polynukleotide Sequenz Durchmischung durch die Fragmentierung und Ligierung von rekursiv zirkulären DNA Molekülen
US10384203B2 (en) 2008-09-24 2019-08-20 First Light Biosciences, Inc. Kits and devices for detecting analytes
JPWO2010064628A1 (ja) * 2008-12-05 2012-05-10 オリンパス株式会社 核酸含有試料の調製方法、試料調製用溶液、及び核酸の解析方法
WO2011113999A1 (en) * 2010-03-15 2011-09-22 University Of Oulu New polypeptides from endophytes of empetrum nigrum and their fragments, encoding them, their production and uses
BR112013032629A2 (pt) 2011-06-19 2017-08-01 Abogen Inc dispositivos, soluções e métodos para coletamento de amostra
CN104185677B (zh) 2011-11-07 2016-03-02 快速微型生物系统公司 用于无菌性测试的盒
CA3171698A1 (en) 2012-04-16 2013-10-24 Rapid Micro Biosystems, Inc. Cell culturing device
WO2015131291A1 (en) 2014-03-07 2015-09-11 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
CN108026566A (zh) * 2015-05-04 2018-05-11 特拉维夫大学拉莫特有限公司 用于使dna片段化的方法和试剂盒
CN106754874A (zh) * 2016-12-06 2017-05-31 浙江指针基因科技有限公司 一种从人体粪便样本中提取dna的试剂盒及其使用方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2010879A1 (en) * 1989-03-07 1990-09-07 Kathleen Potter Speciation of mycobacteria by pulsed-field gel electrophoresis of chromosomal dna fragments
EP0512181A1 (de) * 1991-05-07 1992-11-11 N.V. Innogenetics S.A. Verfahren zum in vitro Nachweis von Chromosomenanomalien, korrelierend mit der CMT1A-Krankheit
WO1994022904A1 (en) * 1993-04-07 1994-10-13 Gradipore Limited Improvements in protein separation
US5872001A (en) * 1994-04-20 1999-02-16 Uab Research Foundation Lanthionine antibiotic compositions and methods
KR20040041639A (ko) * 1995-04-24 2004-05-17 크로막솜 코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
EP0851938A1 (de) 1995-09-22 1998-07-08 Terragen Diversity Inc. Verfahren zur isolierung von xylanase-gensequenzen von dns aus erde, zusammensetzungen verwendbar in diesem verfahren und die damit enthaltendemn zubereitungen
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US5965128A (en) * 1997-08-13 1999-10-12 University Of Georgia Research Foundation Inc. Control of enterohemorrhagic E. coli 0157:H7 in cattle by probiotic bacteria and specific strains of E. coli
EP0939118A1 (de) 1998-02-20 1999-09-01 Universiteit Maastricht Verfahren zur Isolierung von DNA und RNA aus Stuhlproben
MXPA00008559A (es) 1998-03-04 2005-06-06 Diversa Corp Produccion y uso de bibliotecas de adn normalizadas.
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant

Also Published As

Publication number Publication date
US20030049675A1 (en) 2003-03-13
EP1278831B1 (de) 2005-11-30
AU2001248447A1 (en) 2001-11-07
US6989249B2 (en) 2006-01-24
CA2407270A1 (fr) 2001-11-01
EP1278831A2 (de) 2003-01-29
WO2001081357A2 (fr) 2001-11-01
FR2808276A1 (fr) 2001-11-02
ATE311445T1 (de) 2005-12-15
DE60115415T2 (de) 2006-06-14
ES2250376T3 (es) 2006-04-16
US20060188967A1 (en) 2006-08-24
WO2001081357A3 (fr) 2002-03-14
CA2407270C (fr) 2010-10-19
DK1278831T3 (da) 2005-12-27
FR2808276B1 (fr) 2004-04-02

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