DE60234022D1 - Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien - Google Patents

Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien

Info

Publication number
DE60234022D1
DE60234022D1 DE60234022T DE60234022T DE60234022D1 DE 60234022 D1 DE60234022 D1 DE 60234022D1 DE 60234022 T DE60234022 T DE 60234022T DE 60234022 T DE60234022 T DE 60234022T DE 60234022 D1 DE60234022 D1 DE 60234022D1
Authority
DE
Germany
Prior art keywords
media
hydratable
electrophoresis
separation
breaked
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60234022T
Other languages
English (en)
Inventor
Regina D Rooney
Bradley S Scoot
Joseph W Amshey
Thomas R Jackson
Sheldon Engelhorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of DE60234022D1 publication Critical patent/DE60234022D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dispersion Chemistry (AREA)
  • Electrostatic Separation (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60234022T 2001-05-10 2002-03-18 Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien Expired - Lifetime DE60234022D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29046401P 2001-05-10 2001-05-10
PCT/US2002/008438 WO2002092200A1 (en) 2001-05-10 2002-03-18 Methods and apparatus for electrophoresis of prior-cast, hydratable separation media

Publications (1)

Publication Number Publication Date
DE60234022D1 true DE60234022D1 (de) 2009-11-26

Family

ID=23116113

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60234022T Expired - Lifetime DE60234022D1 (de) 2001-05-10 2002-03-18 Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien

Country Status (9)

Country Link
US (2) US6936150B2 (de)
EP (1) EP1385603B1 (de)
JP (1) JP4236938B2 (de)
AT (1) ATE445454T1 (de)
CA (1) CA2445539A1 (de)
DE (1) DE60234022D1 (de)
IL (2) IL158298A0 (de)
NZ (1) NZ530022A (de)
WO (1) WO2002092200A1 (de)

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DE69839553D1 (de) 1997-01-08 2008-07-10 Invitrogen Corp Verfahren zur herstellung von proteinen
US7517442B1 (en) 1999-08-09 2009-04-14 Life Technologies Corporation Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats
AUPQ276099A0 (en) * 1999-09-10 1999-10-07 Proteome Systems Ltd Electrophoresis apparatus and a method of using the same
EP1385603B1 (de) * 2001-05-10 2009-10-14 Life Technologies Corporation Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien
US7601251B2 (en) * 2001-05-10 2009-10-13 Life Technologies Corporation Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
US7622028B2 (en) 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004113899A1 (en) 2003-06-17 2004-12-29 Invitrogen Corporation Chamber-forming electrophoresis cassette cover
AU2003278178A1 (en) * 2003-09-24 2005-04-11 Agilent Technologies, Inc. Electrophoretic separation of amphoteric molecules
US7781173B2 (en) * 2003-09-25 2010-08-24 Life Technologies Corporation Homogeneous populations of molecules
US20050103629A1 (en) * 2003-10-07 2005-05-19 Tom Diller Isoelectric focusing gels and methods of use thereof
WO2005054548A2 (en) 2003-11-28 2005-06-16 Invitrogen Corporation Methods, compositions, and kits for protein crystallization
EP1547690B1 (de) * 2003-12-12 2011-07-06 Becton, Dickinson and Company Verfahren zur Befestigung einer Membran mittels Heissschmelzen ohne Klebstoff
SE0402375D0 (sv) * 2004-09-30 2004-09-30 Amersham Biosciences Ab Method and device for isoelectric focusing
WO2006113365A2 (en) * 2005-04-15 2006-10-26 Invitrogen Corporation Expanding cam lock for sealing slab gels in an electrophoresis apparatus
JP2009544974A (ja) 2006-07-21 2009-12-17 ライフ テクノロジーズ コーポレーション 鮮明に分解する標識タンパク質分子量標準
JP5008064B2 (ja) * 2006-11-24 2012-08-22 独立行政法人産業技術総合研究所 唾液成分のオンチップ分析方法
US20110100821A1 (en) * 2007-04-20 2011-05-05 Brian Furmanski Mineral oil free isoelectric focusing apparatus for immobilized ph gradient strips
WO2009127911A1 (en) * 2008-04-14 2009-10-22 Hafid Mezdour Apparatus and method for non immersed gel electrophoresis
US8262889B2 (en) * 2009-02-20 2012-09-11 Bio-Rad Laboratories, Inc. Unitary electrode and electrode support for horizontal electrophoresis
US9494552B2 (en) * 2012-02-07 2016-11-15 Research Foundation Of The City University Of New York Method and system for documenting an electrophoresis gel
WO2015084887A2 (en) 2013-12-02 2015-06-11 Bio-Rad Laboratories, Inc. Mini-gel comb
USD792603S1 (en) 2015-11-13 2017-07-18 Life Technologies Corporation Rig for electrophoresis gel casting
USD856528S1 (en) * 2015-11-13 2019-08-13 Life Technologies Corporation Cassette clamp for electrophoresis gel casting
USD851779S1 (en) 2015-11-13 2019-06-18 Life Technologies Corporation Electrophoresis cassette with sample loading guide
USD849963S1 (en) 2015-11-13 2019-05-28 Life Technologies Corporation Electrophoresis gel cassette
US10359396B2 (en) 2015-11-13 2019-07-23 Life Technologies Corporation Preparation of electrophoresis gels, and related devices, systems, and methods
USD859688S1 (en) 2015-11-13 2019-09-10 Life Technologies Corporation Electrophoresis slab gel sample loading guide
USD816865S1 (en) 2015-11-13 2018-05-01 Life Technologies Corporation Cassette support base for electrophoresis gel casting
FI127029B (fi) * 2015-12-22 2017-10-13 Outotec Finland Oy Sähkökemiallinen reaktori, vedenkäsittelylaitteisto ja -järjestelmä sekä menetelmä vedenkäsittelylaitteiston ohjaamiseksi

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SE422371B (sv) * 1975-12-12 1982-03-01 Aminkemi Ab Sett att generera en ph-funktion for anvendning vid elektrofores
US4337131A (en) * 1978-11-13 1982-06-29 C. Desaga Gmbh Nachf. Erich Fecht Process for electrophoresis
US4417967A (en) * 1981-11-24 1983-11-29 Georgetown University Grooved gel
US4385974A (en) * 1982-06-24 1983-05-31 Jerry Shevitz Electrophoretic system and method for multidimensional analysis
US4443319A (en) * 1982-09-30 1984-04-17 E. I. Du Pont De Nemours And Company Device for electrophoresis
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US5159049A (en) * 1991-04-22 1992-10-27 Allen Robert C Method for stabilizing polyacrylamide gels
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US5746901A (en) * 1996-04-05 1998-05-05 Regents Of The University Of California Hybrid slab-microchannel gel electrophoresis system
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AUPQ276099A0 (en) * 1999-09-10 1999-10-07 Proteome Systems Ltd Electrophoresis apparatus and a method of using the same
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EP1385603B1 (de) * 2001-05-10 2009-10-14 Life Technologies Corporation Verfahren und vorrichtungen zur elektrophorese von vorgegossenen, hydratisierbaren trennmedien

Also Published As

Publication number Publication date
JP4236938B2 (ja) 2009-03-11
US6936150B2 (en) 2005-08-30
NZ530022A (en) 2005-09-30
CA2445539A1 (en) 2002-11-21
WO2002092200A8 (en) 2004-02-05
JP2004535565A (ja) 2004-11-25
WO2002092200A1 (en) 2002-11-21
EP1385603A4 (de) 2005-01-12
EP1385603A1 (de) 2004-02-04
US20050284764A1 (en) 2005-12-29
ATE445454T1 (de) 2009-10-15
EP1385603B1 (de) 2009-10-14
IL158298A0 (en) 2004-05-12
US20030015426A1 (en) 2003-01-23
IL165848A0 (en) 2006-01-15

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