AU2001248447A1 - Method for indirectly extracting non-cultivable dna organisms and dna obtainableby said method - Google Patents

Method for indirectly extracting non-cultivable dna organisms and dna obtainableby said method

Info

Publication number
AU2001248447A1
AU2001248447A1 AU2001248447A AU4844701A AU2001248447A1 AU 2001248447 A1 AU2001248447 A1 AU 2001248447A1 AU 2001248447 A AU2001248447 A AU 2001248447A AU 4844701 A AU4844701 A AU 4844701A AU 2001248447 A1 AU2001248447 A1 AU 2001248447A1
Authority
AU
Australia
Prior art keywords
dna
organisms
obtainableby
cultivable
extracting non
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001248447A
Inventor
Renaud Nalin
Patrick Robe
Van Tran Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Givaudan France SAS
Original Assignee
Libragen SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Libragen SA filed Critical Libragen SA
Publication of AU2001248447A1 publication Critical patent/AU2001248447A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

A method for the indirect extraction of DNA greater than 300 kb in size from non-cultivatable organisms contained in an environmental sample is disclosed. The method comprises isolating the organisms from the sample and embedding the isolated organisms in a block of agarose where the organisms are subsequently lysed and the DNA subjected to a first alternating field electrophoresis to extract DNA fragments which are large in size and separate them from the cell debris. The first electrophoretic migration is followed by an enzymatic restriction step and additional electrophoretic migrations. The invention also encompasses DNA obtained by the method.
AU2001248447A 2000-04-26 2001-04-03 Method for indirectly extracting non-cultivable dna organisms and dna obtainableby said method Abandoned AU2001248447A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0005274A FR2808276B1 (en) 2000-04-26 2000-04-26 METHOD FOR INDIRECT EXTRACTION OF DNA FROM NON-CULTIVABLE ORGANISMS AND DNA LIKELY TO BE OBTAINED BY SAID PROCESS
FR0005274 2000-04-26
PCT/FR2001/000992 WO2001081357A2 (en) 2000-04-26 2001-04-03 Method for indirectly extracting non-cultivable dna organisms and dna obtainable by said method

Publications (1)

Publication Number Publication Date
AU2001248447A1 true AU2001248447A1 (en) 2001-11-07

Family

ID=8849580

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001248447A Abandoned AU2001248447A1 (en) 2000-04-26 2001-04-03 Method for indirectly extracting non-cultivable dna organisms and dna obtainableby said method

Country Status (10)

Country Link
US (2) US6989249B2 (en)
EP (1) EP1278831B1 (en)
AT (1) ATE311445T1 (en)
AU (1) AU2001248447A1 (en)
CA (1) CA2407270C (en)
DE (1) DE60115415T2 (en)
DK (1) DK1278831T3 (en)
ES (1) ES2250376T3 (en)
FR (1) FR2808276B1 (en)
WO (1) WO2001081357A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE470859T1 (en) 2001-09-06 2010-06-15 Straus Holdings Inc FAST AND SENSITIVE DETECTION OF MOLECULES
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
EP1386966A1 (en) 2002-07-24 2004-02-04 Libragen Method for the expression of unknown environmental DNA into adapted host cells
WO2004018673A2 (en) * 2002-08-20 2004-03-04 B.R.A.I.N. Biotechnology Research And Information Network Ag Isolation and cloning of dna from uncultivated organisms
CA2526985A1 (en) * 2003-05-16 2004-12-02 Wisconsin Alumni Research Foundation Method for isolating and cloning high molecular weight polynucleotide molecules from the environment
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
EP1842915A1 (en) 2006-04-04 2007-10-10 Libragen Method of in vitro polynucleotide sequences shuffling by recursive circular DNA molecules fragmentation and ligation
US10384203B2 (en) 2008-09-24 2019-08-20 First Light Biosciences, Inc. Kits and devices for detecting analytes
JPWO2010064628A1 (en) * 2008-12-05 2012-05-10 オリンパス株式会社 Nucleic acid-containing sample preparation method, sample preparation solution, and nucleic acid analysis method
WO2011113999A1 (en) * 2010-03-15 2011-09-22 University Of Oulu New polypeptides from endophytes of empetrum nigrum and their fragments, encoding them, their production and uses
BR112013032629A2 (en) 2011-06-19 2017-08-01 Abogen Inc devices, solutions and methods for sample collection
CN104185677B (en) 2011-11-07 2016-03-02 快速微型生物系统公司 For the box of sterility test
CA3171698A1 (en) 2012-04-16 2013-10-24 Rapid Micro Biosystems, Inc. Cell culturing device
WO2015131291A1 (en) 2014-03-07 2015-09-11 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
CN108026566A (en) * 2015-05-04 2018-05-11 特拉维夫大学拉莫特有限公司 For making the method and kit of DNA fragmentation
CN106754874A (en) * 2016-12-06 2017-05-31 浙江指针基因科技有限公司 The kit and its application method of DNA are extracted in a kind of sample from human excrement and urine

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2010879A1 (en) * 1989-03-07 1990-09-07 Kathleen Potter Speciation of mycobacteria by pulsed-field gel electrophoresis of chromosomal dna fragments
EP0512181A1 (en) * 1991-05-07 1992-11-11 N.V. Innogenetics S.A. Process for the in vitro diagnosis of chromosomal anomalies liable to be correlated with CMT1A disease
WO1994022904A1 (en) * 1993-04-07 1994-10-13 Gradipore Limited Improvements in protein separation
US5872001A (en) * 1994-04-20 1999-02-16 Uab Research Foundation Lanthionine antibiotic compositions and methods
KR20040041639A (en) * 1995-04-24 2004-05-17 크로막솜 코포레이티드 Methods for generating and screening noverl metabolic pathways
EP0851938A1 (en) 1995-09-22 1998-07-08 Terragen Diversity Inc. Method for isolating xylanase gene sequences from soil dna, compositions useful in such method and compositions obtained thereby
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US5965128A (en) * 1997-08-13 1999-10-12 University Of Georgia Research Foundation Inc. Control of enterohemorrhagic E. coli 0157:H7 in cattle by probiotic bacteria and specific strains of E. coli
EP0939118A1 (en) 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
MXPA00008559A (en) 1998-03-04 2005-06-06 Diversa Corp Production and use of normalized dna libraries.
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant

Also Published As

Publication number Publication date
US20030049675A1 (en) 2003-03-13
EP1278831B1 (en) 2005-11-30
US6989249B2 (en) 2006-01-24
CA2407270A1 (en) 2001-11-01
EP1278831A2 (en) 2003-01-29
WO2001081357A2 (en) 2001-11-01
FR2808276A1 (en) 2001-11-02
ATE311445T1 (en) 2005-12-15
DE60115415T2 (en) 2006-06-14
ES2250376T3 (en) 2006-04-16
US20060188967A1 (en) 2006-08-24
WO2001081357A3 (en) 2002-03-14
DE60115415D1 (en) 2006-01-05
CA2407270C (en) 2010-10-19
DK1278831T3 (en) 2005-12-27
FR2808276B1 (en) 2004-04-02

Similar Documents

Publication Publication Date Title
AU2001248447A1 (en) Method for indirectly extracting non-cultivable dna organisms and dna obtainableby said method
GB9425138D0 (en) Isolation of nucleic acid
WO2002092200A8 (en) Methods and apparatus for electrophoresis of prior-cast, hydratable separation media
AU6460398A (en) Detection of water-borne parasites using electrochemiluminescence
NO20032209L (en) Method of separating and detecting proteins by electrophoresis
DE60314898D1 (en) COMPOSITIONS AND METHOD FOR SEPARATING CELLS
WO2005054263A3 (en) Analyte identification in transformed electropherograms
US5817225A (en) Electrophoresis system for the purification, concentration and size fractionation of nucleic acids
Wang et al. Removal of lipid contaminants by organic solvents from oilseed protein extract prior to electrophoresis
Cavecci-Mendonça et al. Study of proteins with mercury in fish from the Amazon region
WO2004012231A3 (en) Method and apparatus for control of mobility-based ion species identification
DE50112881D1 (en) METHOD FOR THE ELECTROPHORETIC SEPARATION OF MEMBRANE PROTEINS
ATE449843T1 (en) ELECTROPHORETIC SEPARATION OF NUCLEIC ACIDS FROM BIOLOGICAL SAMPLES AT LOW PH
Bhadauria et al. Optimization of a protein extraction technique for fungal proteomics
WO2001061036A8 (en) A method of mapping restriction endonuclease cleavage sites
WO2000040757A3 (en) Method of identifying nucleic acids
ATE274185T1 (en) METHOD AND COMPOSITIONS FOR SEPARATING BIOLOGICAL MACROMOLECLES
AU2003270814A8 (en) Method of extracting nucleic acids from microbial samples
Li et al. Analysis of soluble proteins in natural Cordyceps sinensis from different producing areas by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis
ATE464380T1 (en) METHOD FOR THE COARSE CLARIFICATION OF CELL DISSOLUTIONS FROM MICROORGANISMS
EP0388053A3 (en) Speciation of mycobacteria by pulsed-field gel electrophoresis of chromosomal dna fragments
WO2002014851A3 (en) Electrophoresis apparatus and method
Egyházi et al. Extraction and fractionation of low molecular weight RNA on the microscale
ATE412173T1 (en) TWO-DIMENSIONAL SEPARATION USING CAPILLARY ELECTROPHORESIS PERFORMED IN A SINGLE CAPILLARY
WO2004104179A3 (en) Method for isolating and cloning high molecular weight polynucleotide molecules from the environment