ATE295875T1 - Verfahren zur in vitro herstellung von knochen - Google Patents

Verfahren zur in vitro herstellung von knochen

Info

Publication number
ATE295875T1
ATE295875T1 AT97200620T AT97200620T ATE295875T1 AT E295875 T1 ATE295875 T1 AT E295875T1 AT 97200620 T AT97200620 T AT 97200620T AT 97200620 T AT97200620 T AT 97200620T AT E295875 T1 ATE295875 T1 AT E295875T1
Authority
AT
Austria
Prior art keywords
culture medium
matrix
cells
vitro
substrate
Prior art date
Application number
AT97200620T
Other languages
English (en)
Inventor
Blitterswijk Clemens Anton Van
Bruijn Joost Dick De
Yvonne Pearl Bovell
Original Assignee
Isotis Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isotis Nv filed Critical Isotis Nv
Application granted granted Critical
Publication of ATE295875T1 publication Critical patent/ATE295875T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3608—Bone, e.g. demineralised bone matrix [DBM], bone powder
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3645—Connective tissue
    • A61L27/365—Bones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3808—Endothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1311—Osteocytes, osteoblasts, odontoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C12N2533/18—Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
AT97200620T 1996-03-01 1997-03-03 Verfahren zur in vitro herstellung von knochen ATE295875T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP96200553 1996-03-01
EP96202536 1996-09-09

Publications (1)

Publication Number Publication Date
ATE295875T1 true ATE295875T1 (de) 2005-06-15

Family

ID=26142564

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97200620T ATE295875T1 (de) 1996-03-01 1997-03-03 Verfahren zur in vitro herstellung von knochen

Country Status (4)

Country Link
US (3) US6152964A (de)
AT (1) ATE295875T1 (de)
CA (1) CA2198978A1 (de)
DE (1) DE69733292T2 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
EP0896825B1 (de) * 1997-08-14 2002-07-17 Sulzer Innotec Ag Zusammensetzung und Vorrichtung zur Reparatur von Knorpelgewebe in vivo bestehend aus Nanokapseln mit osteoinduktiven und/oder chondroinduktiven Faktoren
US20020034819A1 (en) * 1998-02-23 2002-03-21 Alan K. Smith Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both; methods for obtaining same; and their uses
US6811776B2 (en) * 2000-12-27 2004-11-02 The Regents Of The University Of Michigan Process for ex vivo formation of mammalian bone and uses thereof
DE10013223C2 (de) * 2000-03-13 2002-07-18 Co Don Ag Verfahren zur in vitro-Herstellung von dreidimensionalem, vitalem Knorpel- oder Knochengewebe und dessen Verwendung als Transplantationsmaterial
US20020114795A1 (en) 2000-12-22 2002-08-22 Thorne Kevin J. Composition and process for bone growth and repair
US20030119771A1 (en) * 2001-08-22 2003-06-26 Rompaey Luc Van Modulators of bone homeostasis identified in a high-throughput screen
WO2003041568A2 (en) * 2001-11-15 2003-05-22 University Of Medicine & Dentistry Of New Jersey A three-dimensional matrix for producing living tissue equivalents
FR2833610B1 (fr) * 2001-12-14 2007-01-26 Natural Implant Construits cellulaires cultives in vitro, preparation et utilisations
US7622562B2 (en) 2002-06-26 2009-11-24 Zimmer Orthobiologics, Inc. Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
US7113320B2 (en) * 2003-02-06 2006-09-26 Evans & Sutherland Computer Corporation GLV based fiber optic transmitter
US20070065803A1 (en) * 2003-06-11 2007-03-22 Centre National De La Recherche Scientifique Patterns for a skeletal system
US8181472B2 (en) * 2005-03-17 2012-05-22 Electrolux Home Products, Inc. Electronic refrigeration control system
US7749555B2 (en) * 2006-01-25 2010-07-06 Medtronic, Inc Modification of chemical forces of bone constructs
US7718616B2 (en) 2006-12-21 2010-05-18 Zimmer Orthobiologics, Inc. Bone growth particles and osteoinductive composition thereof
DE102007005946A1 (de) * 2007-02-01 2008-08-14 Stiftung Caesar Therapeutische Zusammensetzung und Verwendung einer zellfreien Substanz
WO2009064956A1 (en) * 2007-11-14 2009-05-22 Osteosphere, Llc A novel tissue culture platform for screening of potential bone remodeling agents
US20100055078A1 (en) * 2008-08-15 2010-03-04 The Government of the U.S.A, as represented by the Department of Veterans Affairs Methods of making a transplantable bone repair system using multipotent stem cells
IL204116A (en) 2010-02-23 2013-10-31 Sheltagen Medical Ltd Three-dimensional implant for the bones and method of production
EP2625264B1 (de) 2010-10-08 2022-12-07 Terumo BCT, Inc. Verfahren und systeme für züchtung und ernte von zellen in einem hohlfaser-bioreaktorsystem mit steuerungsbedingungen
JP2013542837A (ja) 2010-11-15 2013-11-28 ジンマー オーソバイオロジクス，インコーポレイティド 骨空隙充填剤
WO2015073913A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
US11008547B2 (en) 2014-03-25 2021-05-18 Terumo Bct, Inc. Passive replacement of media
US20160090569A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled Feed
US9238090B1 (en) 2014-12-24 2016-01-19 Fettech, Llc Tissue-based compositions
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
EP3464565A4 (de) 2016-05-25 2020-01-01 Terumo BCT, Inc. Zellexpansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352887A (en) * 1979-10-29 1982-10-05 Albert Einstein College Of Medicine Of Yeshiva University Method and article for culturing differentiated cells
US4314380A (en) * 1980-09-26 1982-02-09 Koken Co., Ltd. Artificial bone
US4485096A (en) * 1982-02-26 1984-11-27 Massachusetts Institute Of Technology Tissue-equivalent and method for preparation thereof
US5628781A (en) * 1985-06-06 1997-05-13 Thomas Jefferson University Implant materials, methods of treating the surface of implants with microvascular endothelial cells, and the treated implants themselves
US4963489A (en) * 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5266480A (en) * 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
US5124316A (en) * 1986-11-14 1992-06-23 President And Fellows Of Harvard College Method for periodontal regeneration
US5041138A (en) * 1986-11-20 1991-08-20 Massachusetts Institute Of Technology Neomorphogenesis of cartilage in vivo from cell culture
US4846835A (en) * 1987-06-15 1989-07-11 Grande Daniel A Technique for healing lesions in cartilage
US4904259A (en) * 1988-04-29 1990-02-27 Samuel Itay Compositions and methods for repair of cartilage and bone
US5226914A (en) * 1990-11-16 1993-07-13 Caplan Arnold I Method for treating connective tissue disorders
US5197985A (en) * 1990-11-16 1993-03-30 Caplan Arnold I Method for enhancing the implantation and differentiation of marrow-derived mesenchymal cells
FR2670215B1 (fr) 1990-12-10 2000-10-13 Lignees de cellules immortalisees et leurs applications, notamment a la production de cellules differenciees.
FR2679250B1 (fr) * 1991-07-19 1995-07-13 Inoteb Utilisation de carbonate de calcium poreux comme materiau support de culture in vitro de cellules eucaryotes.
GB9125052D0 (en) * 1991-11-26 1992-01-22 Isis Innovation Culture of bone cells
EP0672117A4 (de) * 1992-08-13 1996-06-12 Univ Pennsylvania Bioaktive materialien zur in vitro inokkulation von zellen - nützlich als implantierbarer knochenersatz.
US5541106A (en) * 1993-04-05 1996-07-30 Desmos, Inc. Cell matrix stimulated attachment and hemidesmosome assembly
US5522895A (en) 1993-07-23 1996-06-04 Rice University Biodegradable bone templates
JP2984176B2 (ja) 1993-12-30 1999-11-29 新田ゼラチン株式会社 骨髄細胞の培養方法、培養用混合物および硬組織欠損部への移植用材料
US5972703A (en) * 1994-08-12 1999-10-26 The Regents Of The University Of Michigan Bone precursor cells: compositions and methods
US5707962A (en) * 1994-09-28 1998-01-13 Gensci Regeneration Sciences Inc. Compositions with enhanced osteogenic potential, method for making the same and therapeutic uses thereof
WO1996014400A1 (en) * 1994-11-08 1996-05-17 Bradley Michael John Stringer Human cell-lines
US5688531A (en) * 1994-12-23 1997-11-18 Ramot University Authority For Applied Research And Industrial Development, Ltd. Method for regulating bone forming cells
PT868505E (pt) * 1995-11-16 2005-06-30 Univ Case Western Reserve Inducao condrogenica in vitro de celulas estaminais mesenquimais humanas
IT1282207B1 (it) * 1995-11-20 1998-03-16 Fidia Advanced Biopolymers Srl Sistemi di coltura di cellule staminali di midollo osseo umano in matrici tridimensionali costituiti da esteri dell'acido ialuronico
EP0877795A4 (de) * 1996-01-16 2003-03-05 Depuy Orthopaedics Inc Isolierung von vorläuferzellen aus hematopoietischen und nicht hematopoietischen gewebe und deren verwendung
AU2730497A (en) * 1996-04-17 1997-11-07 Case Western Reserve University Cryopreservation and extensive subculturing of human mesenchymal stem cells
AU731468B2 (en) * 1996-04-19 2001-03-29 Mesoblast International Sarl Regeneration and augmentation of bone using mesenchymal stem cells

Also Published As

Publication number Publication date
DE69733292T2 (de) 2006-04-27
US6152964A (en) 2000-11-28
CA2198978A1 (en) 1997-09-01
DE69733292D1 (de) 2005-06-23
US20010032016A1 (en) 2001-10-18
US6299650B1 (en) 2001-10-09

Similar Documents

Publication Publication Date Title
ATE295875T1 (de) Verfahren zur in vitro herstellung von knochen
US5197985A (en) Method for enhancing the implantation and differentiation of marrow-derived mesenchymal cells
US5226914A (en) Method for treating connective tissue disorders
EP3213778B1 (de) Zellen auf matrixunterlagen für die wiederherstellung von geweben
US4883487A (en) Method of producing an artifical skin
Rai et al. Tissue engineering: step ahead in maxillofacial reconstruction
WO2003028545A3 (en) Method, instruments, and kit for autologous transplantation
BR0214217A (pt) Aumento de função de órgão
NO991271L (no) Benmorfogenetiske Protein-16-sammensetninger (BMP-16)
DE60238416D1 (de) Plasmaprotein-matritzes und verfahren zu ihrer herstellung
AU1615599A (en) Differentiation of adipose stromal cells into osteoblasts and uses thereof
DE50112855D1 (de) Verfahren zur Herstellung von Knorpelimplantaten mittels in vitro gezüchteter Chondrozyten
JPS59192364A (ja) 軟骨および骨の修復方法
DK0477290T3 (da) Fremgangsmåder, sammensætninger og indretninger til dyrkning af celler
JP6814631B2 (ja) 骨形成幹細胞を含む骨移植片
ATE255920T1 (de) Methoden und zusammensetzungen zur heilung und reparatur von gelenkknorpeln
DE50101920D1 (de) Verfahren zur herstellung eines bioartifiziellen transplantates
ATE457752T1 (de) Gezüchtete knorpelzellen beinhaltendes implantat und verfahren zur herstellung
CN110958892A (zh) 软骨移植物支架
DE69535263D1 (de) Angiotensin ii zur verbesserung der befruchtung
JPH0669487B2 (ja) 生体細胞の成長並びに機能分化の促進・制御方法
MXPA03009312A (es) Metodo para trasplante autologo.
DE69837212D1 (de) Verfahren zur induzierung der osteoblastischen differenzierung der zellen des menschlichen extramedullären fettgewebes
ATE341630T1 (de) Verwendung von pankreatischer procarboxypeptidase b zur herstellung von insulin
Krečič Stres et al. Mesenchymal stem cells: a modern approach to treat long bones defects

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties