ATE256484T1 - Einbringen von makromolekülen in zellen - Google Patents

Einbringen von makromolekülen in zellen

Info

Publication number
ATE256484T1
ATE256484T1 AT00903076T AT00903076T ATE256484T1 AT E256484 T1 ATE256484 T1 AT E256484T1 AT 00903076 T AT00903076 T AT 00903076T AT 00903076 T AT00903076 T AT 00903076T AT E256484 T1 ATE256484 T1 AT E256484T1
Authority
AT
Austria
Prior art keywords
electrodes
cells
macromolecules
electrode assembly
waveform generator
Prior art date
Application number
AT00903076T
Other languages
English (en)
Inventor
Alan D King
Richard E Walters
Original Assignee
Cyto Pulse Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cyto Pulse Sciences Inc filed Critical Cyto Pulse Sciences Inc
Application granted granted Critical
Publication of ATE256484T1 publication Critical patent/ATE256484T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/325—Applying electric currents by contact electrodes alternating or intermittent currents for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/20—Applying electric currents by contact electrodes continuous direct currents
    • A61N1/30—Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
    • A61N1/303—Constructional details
    • A61N1/306—Arrangements where at least part of the apparatus is introduced into the body

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Electrotherapy Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT00903076T 1999-01-28 2000-01-12 Einbringen von makromolekülen in zellen ATE256484T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11775599P 1999-01-28 1999-01-28
PCT/US2000/000014 WO2000044438A1 (en) 1999-01-28 2000-01-12 Delivery of macromolecules into cells

Publications (1)

Publication Number Publication Date
ATE256484T1 true ATE256484T1 (de) 2004-01-15

Family

ID=22374647

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00903076T ATE256484T1 (de) 1999-01-28 2000-01-12 Einbringen von makromolekülen in zellen

Country Status (8)

Country Link
US (1) US6603998B1 (de)
EP (1) EP1163024B1 (de)
JP (1) JP4499295B2 (de)
CN (1) CN1191872C (de)
AT (1) ATE256484T1 (de)
CA (1) CA2372409C (de)
DE (1) DE60007290T2 (de)
WO (1) WO2000044438A1 (de)

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Also Published As

Publication number Publication date
CN1191872C (zh) 2005-03-09
DE60007290T2 (de) 2004-09-23
WO2000044438A1 (en) 2000-08-03
CA2372409A1 (en) 2000-08-03
EP1163024A4 (de) 2002-10-24
US6603998B1 (en) 2003-08-05
JP2002535100A (ja) 2002-10-22
CA2372409C (en) 2005-03-29
EP1163024A1 (de) 2001-12-19
JP4499295B2 (ja) 2010-07-07
CN1346290A (zh) 2002-04-24
DE60007290D1 (de) 2004-01-29
EP1163024B1 (de) 2003-12-17

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