ATE239089T1 - Gezielte punkt-mutagenese von dna - Google Patents

Gezielte punkt-mutagenese von dna

Info

Publication number
ATE239089T1
ATE239089T1 AT93901907T AT93901907T ATE239089T1 AT E239089 T1 ATE239089 T1 AT E239089T1 AT 93901907 T AT93901907 T AT 93901907T AT 93901907 T AT93901907 T AT 93901907T AT E239089 T1 ATE239089 T1 AT E239089T1
Authority
AT
Austria
Prior art keywords
dna
mutant
site
parental
circular
Prior art date
Application number
AT93901907T
Other languages
English (en)
Inventor
Jac A Nickoloff
Frank A Ray
Win P Deng
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Application granted granted Critical
Publication of ATE239089T1 publication Critical patent/ATE239089T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT93901907T 1991-12-24 1992-12-17 Gezielte punkt-mutagenese von dna ATE239089T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81358191A 1991-12-24 1991-12-24
PCT/US1992/011076 WO1993013216A1 (en) 1991-12-24 1992-12-17 Site-directed mutagenesis of dna

Publications (1)

Publication Number Publication Date
ATE239089T1 true ATE239089T1 (de) 2003-05-15

Family

ID=25212814

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93901907T ATE239089T1 (de) 1991-12-24 1992-12-17 Gezielte punkt-mutagenese von dna

Country Status (7)

Country Link
US (1) US5354670A (de)
EP (1) EP0620858B1 (de)
JP (1) JP3398957B2 (de)
AT (1) ATE239089T1 (de)
CA (1) CA2126438C (de)
DE (1) DE69233035D1 (de)
WO (1) WO1993013216A1 (de)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6043296A (en) * 1995-06-06 1996-12-24 Yale University Chemically modified oligonucleotide for site-directed mutage nesis
WO1997012993A1 (en) * 1995-10-02 1997-04-10 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
US5780270A (en) * 1996-07-17 1998-07-14 Promega Corporation Site-specific mutagenesis and mutant selection utilizing antibiotic-resistant markers encoding gene products having altered substrate specificity
EP2208789B1 (de) 1997-03-12 2015-07-22 Applied Biosystems, LLC DNA Polymerasen mit verbesserten Eigenschaften für den Einbau markierter Nukleotide
CA2222993A1 (en) * 1998-02-04 1999-08-04 The Ontario Cancer Institute A method for using a ribosome-inactivating protein complex as a structural template and a molecular search engine in the design, construction and screening of combinatorial protein libraries
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
FR2789696A1 (fr) * 1999-02-12 2000-08-18 Biomethodes Procede de mutagenese d'un fragment d'acide nucleique par clonage et polymerisation-ligation
GB9906738D0 (en) * 1999-03-23 1999-05-19 Plant Bioscience Ltd Mutagenesis
JP3859947B2 (ja) * 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
US20020155439A1 (en) * 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
SE0102327D0 (sv) 2001-06-28 2001-06-28 Active Biotech Ab A novel engineered superantigen for human therapy
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
KR20130012595A (ko) 2002-08-12 2013-02-04 제네렉스, 인코포레이티드 폭스바이러스 및 암과 관련된 방법 및 조성물
US7785799B2 (en) * 2002-08-16 2010-08-31 The Board Of Regents Of The University Of Texas System Compositions and methods related to flavivirus envelope protein domain III antigens
DE60335997D1 (de) 2002-09-16 2011-03-24 Agennix Inc Lactoferrin-zusammensetzungen und verfahren zur behandlung von diabetischen ulcus
WO2004089981A2 (en) 2003-04-02 2004-10-21 Board Of Regents, The University Of Texas System Antitumor effect of mutant bik
US7354715B2 (en) * 2003-05-22 2008-04-08 Dow Agrosciences Llc High-throughput methods of screening DNA for deletions and other mutations
GB0318110D0 (en) * 2003-08-01 2003-09-03 Isaeo Ltd Methods and kits for detecting an enzyme capable of modifying a nucleic acid
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
CA2593417C (en) * 2005-01-10 2014-10-28 Ralph Raymond Minter Method of mutagenesis
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
US20070026012A1 (en) 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
EP1933857A2 (de) * 2005-09-07 2008-06-25 Jennerex Biotherapeutics ULC Systemische behandlung von metastasierten und/oder systemisch dissemierten tumoren mit gm-csf-exprimierenden pockenviren
CA2664549A1 (en) * 2005-09-26 2007-03-29 University Health Network Library from toxin mutants, and methods of using same
US8658608B2 (en) * 2005-11-23 2014-02-25 Yale University Modified triple-helix forming oligonucleotides for targeted mutagenesis
EP1984738A2 (de) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Mikrofluidische vorrichtungen und verfahren zur verwendung bei der bildung und kontrolle von nanoreaktoren
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2064229B1 (de) 2006-09-15 2015-08-12 Ottawa Hospital Research Institute Onkolytisches rhabdovirus
BRPI0717474A2 (pt) * 2006-10-20 2014-03-11 Arizona Board Of Regentes For And On Behalf Of Arizona State University Cianobactéria modificada
WO2008086529A2 (en) * 2007-01-11 2008-07-17 Yale University Compositions and methods for targeted inactivation of hiv cell surface receptors
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20100267147A1 (en) * 2007-04-25 2010-10-21 GM Biosciences, Inc. Site-directed mutagenesis in circular methylated dna
DK3031919T3 (en) * 2007-07-31 2019-01-07 Basf Enzymes Llc Tailored multi-site combination device
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP2477499B1 (de) 2009-09-14 2018-04-11 SillaJen Biotherapeutics, Inc. Krebstherapie mit einer kombination aus onkolytischen impfstoffen
MX365946B (es) 2009-12-10 2019-06-19 Turnstone Lp Rabdovirus oncolitico.
EP4484577A3 (de) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digitale analytanalyse
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
KR101942237B1 (ko) 2011-01-04 2019-01-25 신라젠(주) 종양 항원에 대한 항체의 생산 및 종양용해 우두 바이러스의 투여에 의한 종양 특이적 보체 의존적 세포독성의 생산
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
EP3709018A1 (de) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtung zum nachweis von komponenten einer chemischen reaktion
WO2012167382A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013038010A2 (en) 2011-09-16 2013-03-21 Lexogen Gmbh Nucleic acid transcription method
WO2013109950A1 (en) * 2012-01-20 2013-07-25 Kennesaw State University Research And Services Foundation Methods to mutate circular deoxyribonucleotides
WO2013134822A1 (en) 2012-03-13 2013-09-19 James Cook University Method for treating inflammation
US10968446B2 (en) 2012-11-01 2021-04-06 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
CN105658790A (zh) 2013-02-21 2016-06-08 东安大略研究所儿童医院有限公司 疫苗组合物
WO2015039189A1 (en) 2013-09-18 2015-03-26 James Cook University Modified anti-inflammatory proteins and method of use
AU2014324093A1 (en) 2013-09-18 2016-04-28 James Cook University Anti-inflammatory proteins and methods of use
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
MX421690B (es) 2015-07-13 2025-03-14 Pivot Bio Inc Metodos y composiciones para mejorar atributos de plantas
WO2017062412A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
US9975926B2 (en) 2015-12-08 2018-05-22 The United States of America, as Represented by the Secretary of Homeland Security Methods of making and using vaccines utilizing minicircle DNA expression vectors for production of foot-and-mouth-disease virus proteins and virus-like particles
AU2017206656B2 (en) 2016-01-10 2024-02-01 Neotx Therapeutics Ltd. Immunopotentiator enhanced superantigen mediated cancer immunotherapy
EP3416976A2 (de) 2016-02-16 2018-12-26 Yale University Zusammensetzungen zur verbesserung von gezielter geneditierung und verfahren zur verwendung davon
JP7234116B2 (ja) 2017-01-12 2023-03-07 ピボット バイオ， インコーポレイテッド 植物形質を改善するための方法および組成物
US20200113821A1 (en) 2017-04-04 2020-04-16 Yale University Compositions and methods for in utero delivery
AU2018354221B2 (en) 2017-10-25 2025-04-17 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
CN117757706A (zh) 2017-10-25 2024-03-26 皮沃特生物股份有限公司 靶向改良植物性状的固氮的基因靶标
US11963530B2 (en) 2018-06-27 2024-04-23 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
CA3105529A1 (en) 2018-07-11 2020-01-16 Pivot Bio, Inc. Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes
WO2020132632A2 (en) 2018-12-21 2020-06-25 Pivot Bio, Inc. Methods, compositions, and media for improving plant traits
BR112021012231A2 (pt) 2018-12-28 2021-09-28 Braskem S.A. Modulação do fluxo de carbono através das vias de meg e c3 para a produção melhorada de monoetileno glicol e compostos c3
AU2020206751A1 (en) 2019-01-07 2021-07-01 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
US12281299B2 (en) 2019-03-19 2025-04-22 Massachusetts Institute Of Technology Control of nitrogen fixation in rhizobia that associate with cereals
WO2020219932A1 (en) 2019-04-24 2020-10-29 Pivot Bio, Inc. Gene targets for nitrogen fixation targeting for improving plant traits
AU2020275142A1 (en) 2019-05-15 2021-12-16 Neotx Therapeutics Ltd. Cancer treatment
EP4013867A4 (de) * 2019-08-16 2023-08-30 Resolution Bioscience, Inc. Gezielte hybride fangverfahren zur bestimmung von t-zell-repertoires
WO2021221690A1 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
WO2022074464A2 (en) 2020-03-05 2022-04-14 Neotx Therapeutics Ltd. Methods and compositions for treating cancer with immune cells
EP4143293A1 (de) 2020-05-01 2023-03-08 Pivot Bio, Inc. Messung der stickstofffixierung und -aufnahme
EP4143211A2 (de) 2020-05-01 2023-03-08 Pivot Bio, Inc. Modifizierte bakterienstämme zur verbesserten fixierung von stickstoff
EP4142477A1 (de) 2020-05-01 2023-03-08 Pivot Bio, Inc. Stabile flüssige formulierungen für stickstofffixierende mikroorganismen
US20230295559A1 (en) 2020-05-13 2023-09-21 Pivot Bio, Inc. De-repression of nitrogen fixation in gram-positive microorganisms
CN118302038A (zh) 2021-07-02 2024-07-05 皮沃特生物股份有限公司 用于改良固氮的基因工程化细菌菌株
CN120583887A (zh) 2022-12-23 2025-09-02 皮沃特生物股份有限公司 各种固氮细菌与各种生物产品的组合用于实现协同效应
WO2025155518A1 (en) 2024-01-18 2025-07-24 Pivot Bio, Inc. Host cells for enhanced plant growth
WO2025155521A1 (en) 2024-01-18 2025-07-24 Pivot Bio, Inc. Host cells for enhanced plant growth

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE142273T1 (de) * 1990-06-08 1996-09-15 Behringwerke Ag Verfahren zur quantitativen bestimmung von dns- sequenzen
DE4024187A1 (de) * 1990-07-30 1992-02-06 Biotechnolog Forschung Gmbh Oligonucleotid-gerichtete mutagenese von plasmiden

Also Published As

Publication number Publication date
EP0620858B1 (de) 2003-05-02
WO1993013216A1 (en) 1993-07-08
DE69233035D1 (de) 2003-06-05
EP0620858A4 (en) 1997-05-07
JPH08500721A (ja) 1996-01-30
EP0620858A1 (de) 1994-10-26
US5354670A (en) 1994-10-11
JP3398957B2 (ja) 2003-04-21
CA2126438C (en) 2003-12-02
CA2126438A1 (en) 1993-07-08

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