ATE239089T1 - Gezielte punkt-mutagenese von dna - Google Patents

Gezielte punkt-mutagenese von dna

Info

Publication number
ATE239089T1
ATE239089T1 AT93901907T AT93901907T ATE239089T1 AT E239089 T1 ATE239089 T1 AT E239089T1 AT 93901907 T AT93901907 T AT 93901907T AT 93901907 T AT93901907 T AT 93901907T AT E239089 T1 ATE239089 T1 AT E239089T1
Authority
AT
Austria
Prior art keywords
dna
mutant
site
parental
circular
Prior art date
Application number
AT93901907T
Other languages
English (en)
Inventor
Jac A Nickoloff
Frank A Ray
Win P Deng
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Application granted granted Critical
Publication of ATE239089T1 publication Critical patent/ATE239089T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT93901907T 1991-12-24 1992-12-17 Gezielte punkt-mutagenese von dna ATE239089T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81358191A 1991-12-24 1991-12-24
PCT/US1992/011076 WO1993013216A1 (en) 1991-12-24 1992-12-17 Site-directed mutagenesis of dna

Publications (1)

Publication Number Publication Date
ATE239089T1 true ATE239089T1 (de) 2003-05-15

Family

ID=25212814

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93901907T ATE239089T1 (de) 1991-12-24 1992-12-17 Gezielte punkt-mutagenese von dna

Country Status (7)

Country Link
US (1) US5354670A (de)
EP (1) EP0620858B1 (de)
JP (1) JP3398957B2 (de)
AT (1) ATE239089T1 (de)
CA (1) CA2126438C (de)
DE (1) DE69233035D1 (de)
WO (1) WO1993013216A1 (de)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6043296A (en) * 1995-06-06 1996-12-24 Yale University Chemically modified oligonucleotide for site-directed mutage nesis
WO1997012993A1 (en) * 1995-10-02 1997-04-10 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
US5780270A (en) * 1996-07-17 1998-07-14 Promega Corporation Site-specific mutagenesis and mutant selection utilizing antibiotic-resistant markers encoding gene products having altered substrate specificity
EP0983364B1 (de) 1997-03-12 2002-06-12 PE Corporation (NY) Dna polymerasen, die verbesserte fähigkeiten zum einbau markierter nukleotide besitzen
CA2222993A1 (en) * 1998-02-04 1999-08-04 The Ontario Cancer Institute A method for using a ribosome-inactivating protein complex as a structural template and a molecular search engine in the design, construction and screening of combinatorial protein libraries
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
FR2789696A1 (fr) * 1999-02-12 2000-08-18 Biomethodes Procede de mutagenese d'un fragment d'acide nucleique par clonage et polymerisation-ligation
GB9906738D0 (en) * 1999-03-23 1999-05-19 Plant Bioscience Ltd Mutagenesis
JP3859947B2 (ja) * 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
US20020155439A1 (en) * 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
US20040259082A1 (en) * 2001-04-24 2004-12-23 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymers
SE0102327D0 (sv) 2001-06-28 2001-06-28 Active Biotech Ab A novel engineered superantigen for human therapy
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
EP2269618A1 (de) 2002-08-12 2011-01-05 Jennerex Biotherapeutics ULC Ein onkolytisches Vaccinia-Virus zur Verwendung in Kombination mit Chemotherapie zur Behandlung von Krebs
WO2004016586A2 (en) * 2002-08-16 2004-02-26 Board Of Regents The University Of Texas System Compositions and methods related to flavivirus envelope protein domain iii antigens
DE60335997D1 (de) 2002-09-16 2011-03-24 Agennix Inc Lactoferrin-zusammensetzungen und verfahren zur behandlung von diabetischen ulcus
WO2004089981A2 (en) 2003-04-02 2004-10-21 Board Of Regents, The University Of Texas System Antitumor effect of mutant bik
CA2526533C (en) * 2003-05-22 2014-01-14 Dow Agrosciences Llc High-throughput methods of screening dna for deletions and other mutations
GB0318110D0 (en) * 2003-08-01 2003-09-03 Isaeo Ltd Methods and kits for detecting an enzyme capable of modifying a nucleic acid
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
DE602006013596D1 (de) * 2005-01-10 2010-05-27 Medimmune Ltd Cambridge Mutageneseverfahren
US20070026012A1 (en) 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
JP2009507853A (ja) * 2005-09-07 2009-02-26 ジェンネレックス インコーポレイティッド Ｇｍ−ｃｓｆ発現ポックスウイルスを用いる転移性および／または全身播種性癌の全身処置
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
EP2402367A1 (de) * 2005-09-26 2012-01-04 Molecular Templates, Inc. Bibliothek aus Toxinmutanten und Verwendungsverfahren dafür
US8658608B2 (en) * 2005-11-23 2014-02-25 Yale University Modified triple-helix forming oligonucleotides for targeted mutagenesis
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
EP2839837B1 (de) 2006-09-15 2019-05-08 Children's Hospital of Eastern Ontario Research Institute Inc. Onkolytisches farmington rhabdovirus
BRPI0717474A2 (pt) * 2006-10-20 2014-03-11 Arizona Board Of Regentes For And On Behalf Of Arizona State University Cianobactéria modificada
US20100172882A1 (en) * 2007-01-11 2010-07-08 Glazer Peter M Compositions and methods for targeted inactivation of hiv cell surface receptors
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20100267147A1 (en) * 2007-04-25 2010-10-21 GM Biosciences, Inc. Site-directed mutagenesis in circular methylated dna
BRPI0815046A2 (pt) 2007-07-31 2014-10-14 Verenium Corp Personalização de montagem combinatória de múltiplos locais
EP2315629B1 (de) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Tröpfchenbibliotheken
JP5879266B2 (ja) 2009-09-14 2016-03-08 シラジェン バイオセラピューティクス インコーポレイテッド 腫瘍崩壊性ワクシニアウイルスの併用癌療法
CA2836117C (en) 2009-12-10 2017-08-15 Ottawa Hospital Research Institute Oncolytic rhabdovirus
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
EP3447155A1 (de) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwichassays in tröpfchen
CA2824277C (en) 2011-01-04 2021-08-31 Jennerex, Inc. Generation of antibodies to tumor antigens and generation of tumor specific complement dependent cytotoxicity by administration of oncolytic vaccinia virus
EP2673614B1 (de) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Verfahren zur bildung gemischter tröpfchen
EP2675819B1 (de) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
WO2012167382A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US10612018B2 (en) 2011-09-16 2020-04-07 Lexogen Gmbh Nucleic acid transcription method
US9476079B2 (en) 2012-01-20 2016-10-25 Kennesaw State University Research And Services Foundation, Inc. Methods to mutate circular deoxyribonucleotides
CA2866819C (en) 2012-03-13 2022-01-04 James Cook University Method for treating inflammation
US10968446B2 (en) 2012-11-01 2021-04-06 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP2958994B1 (de) 2013-02-21 2019-05-29 Turnstone Limited Partnership Impfstoffzusammensetzung
JP2016536343A (ja) 2013-09-18 2016-11-24 ジェームス・クック・ユニバーシティー 抗炎症性のタンパク質及び使用方法
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
KR20230152796A (ko) 2015-07-13 2023-11-03 피벗 바이오, 인크. 식물 형질 개선을 위한 방법 및 조성물
JP2018537119A (ja) 2015-10-05 2018-12-20 マサチューセッツ インスティテュート オブ テクノロジー リファクターされたｎｉｆクラスターを使用する窒素固定
US9975926B2 (en) 2015-12-08 2018-05-22 The United States of America, as Represented by the Secretary of Homeland Security Methods of making and using vaccines utilizing minicircle DNA expression vectors for production of foot-and-mouth-disease virus proteins and virus-like particles
WO2017122098A2 (en) 2016-01-10 2017-07-20 Neotx Therapeutics Ltd. Methods and compositions for enhancing the potency of superantigen mediated cancer immunotherapy.
CN109476706A (zh) 2016-02-16 2019-03-15 耶鲁大学 用于促进靶向基因编辑的组合物及其使用方法
MX2019008285A (es) 2017-01-12 2019-11-11 Pivot Bio Inc Métodos y composiciones para mejorar atributos de plantas.
US11993778B2 (en) 2017-10-25 2024-05-28 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
CN112739668A (zh) 2018-06-27 2021-04-30 皮沃特生物股份有限公司 包括重构固氮微生物的农业组合物
EP3897105A2 (de) 2018-12-21 2021-10-27 Pivot Bio, Inc. Verfahren, zusammensetzungen und medien zur verbesserung von pflanzenmerkmalen
BR112021012231A2 (pt) 2018-12-28 2021-09-28 Braskem S.A. Modulação do fluxo de carbono através das vias de meg e c3 para a produção melhorada de monoetileno glicol e compostos c3
AU2020261427A1 (en) 2019-04-24 2021-11-11 Pivot Bio, Inc. Gene targets for nitrogen fixation targeting for improving plant traits
EA202193121A1 (ru) 2019-05-15 2022-02-21 Неоткс Терапьютикс Лтд. Лечение рака
WO2021221690A1 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
AU2021357520A1 (en) 2020-03-05 2022-09-29 Neotx Therapeutics Ltd. Methods and compositions for treating cancer with immune cells
US20230175959A1 (en) 2020-05-01 2023-06-08 Pivot Bio, Inc. Measurement of nitrogen fixation and incorporation
EP4143211A2 (de) 2020-05-01 2023-03-08 Pivot Bio, Inc. Modifizierte bakterienstämme zur verbesserten fixierung von stickstoff
WO2021231449A2 (en) 2020-05-13 2021-11-18 Pivot Bio, Inc. De-repression of nitrogen fixation in gram-positive microorganisms
WO2023278804A1 (en) 2021-07-02 2023-01-05 Pivot Bio, Inc. Genetically-engineered bacterial strains for improved fixation of nitrogen

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE142273T1 (de) * 1990-06-08 1996-09-15 Behringwerke Ag Verfahren zur quantitativen bestimmung von dns- sequenzen
DE4024187A1 (de) * 1990-07-30 1992-02-06 Biotechnolog Forschung Gmbh Oligonucleotid-gerichtete mutagenese von plasmiden

Also Published As

Publication number Publication date
DE69233035D1 (de) 2003-06-05
EP0620858B1 (de) 2003-05-02
US5354670A (en) 1994-10-11
EP0620858A4 (en) 1997-05-07
CA2126438C (en) 2003-12-02
EP0620858A1 (de) 1994-10-26
WO1993013216A1 (en) 1993-07-08
JP3398957B2 (ja) 2003-04-21
CA2126438A1 (en) 1993-07-08
JPH08500721A (ja) 1996-01-30

Similar Documents

Publication Publication Date Title
ATE239089T1 (de) Gezielte punkt-mutagenese von dna
EP0760002A4 (de) Die bestimmung von mutationen durch spaltung mit resolvase
MY133278A (en) Method of producing l-lysine by fermentation
ATE59410T1 (de) Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer.
DE69432818D1 (de) Enzym mit proteaseaktivität
DE69203992T2 (de) Zusammensetzung und verfahren zur entfernung oder vermeidung von biobelag.
ATE71754T1 (de) Verfahren und vorrichtung zum synchronisieren des herstellens von getrennten, freilaufenden und zeitabhaengigen kennziffern.
ATE31087T1 (de) Chemisches additions- oder substraktionsverfahren.
HK1089425A1 (en) Treatment of a waste stream through production and utilization of oxyhydrogen gas
AR017023A1 (es) Tipeo genomico de adn metiltransferasa
DE69636895D1 (de) Die Verwendung der 3'-wesentlichen Bearbeitungswirksamkeit von DNS-Polymerase
ATE202584T1 (de) Verfahren zur herstellung von expandierbaren styrolpolymerisaten
IL124794A0 (en) Method of screening for enzyme activity
ATE148745T1 (de) Kontrolle der mikrosporogenese durch verwendung von externinduzierbaren promotorsequenzen
Reanney Genetic error and genome design
DE69531301D1 (de) Dna kodierend für die bacillus licheniformis pwd-1 keratinase
Nickoloff et al. Site-directed mutagenesis of DNA
FI933413A0 (fi) Foerfarande foer framstaellning av rekombinant, biologiskt aktivt eukaryotiskt alkaliskt fosfatas
Abdurashitov et al. BstF5I, an unusual isoschizomer of FokI
ATE13910T1 (de) Verfahren zur zuechtung von humanen fibroblasten und fibroblastenartigen zellen.
FI885053A0 (fi) Fluorerade arakidonsyraderivat.
Arber Cultural aspects of the theory of molecular evolution
DE69015843D1 (de) Cytochrom-C-Gen aus einer Wasserstoffbakterie.
SE8703767D0 (sv) Plasmid having vanillic acid decomposing ability and process of producing the same
Kuester Electrical power and fuels production from wastes

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties