ATE193330T1 - Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymen - Google Patents

Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymen

Info

Publication number
ATE193330T1
ATE193330T1 AT94914164T AT94914164T ATE193330T1 AT E193330 T1 ATE193330 T1 AT E193330T1 AT 94914164 T AT94914164 T AT 94914164T AT 94914164 T AT94914164 T AT 94914164T AT E193330 T1 ATE193330 T1 AT E193330T1
Authority
AT
Austria
Prior art keywords
sample
enzymatically active
solid support
assaying
indicator
Prior art date
Application number
AT94914164T
Other languages
English (en)
Inventor
Paul J Lawrence
Aulena Chauhuri
Terrence J Andreasen
Original Assignee
Litmus Concepts Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Litmus Concepts Inc filed Critical Litmus Concepts Inc
Priority claimed from PCT/US1994/004098 external-priority patent/WO1994024306A1/en
Application granted granted Critical
Publication of ATE193330T1 publication Critical patent/ATE193330T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4731—Casein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/795—Porphyrin- or corrin-ring-containing peptides
    • G01N2333/805—Haemoglobins; Myoglobins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT94914164T 1993-04-14 1994-04-13 Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymen ATE193330T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/048,536 US5416003A (en) 1993-04-14 1993-04-14 Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities
CN94192191A CN1096501C (zh) 1993-04-14 1994-04-13 报告酶释放技术∶分析天冬氨酸蛋白酶及其它水解酶活性是否存在的方法
PCT/US1994/004098 WO1994024306A1 (en) 1993-04-14 1994-04-13 Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities

Publications (1)

Publication Number Publication Date
ATE193330T1 true ATE193330T1 (de) 2000-06-15

Family

ID=37698030

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94914164T ATE193330T1 (de) 1993-04-14 1994-04-13 Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymen

Country Status (6)

Country Link
US (3) US5416003A (de)
EP (1) EP0694077B1 (de)
CN (1) CN1096501C (de)
AT (1) ATE193330T1 (de)
DE (1) DE69424663T2 (de)
RU (1) RU2159818C2 (de)

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US5416003A (en) * 1993-04-14 1995-05-16 Litmus Concepts, Inc. Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities
AU721069B2 (en) * 1993-04-14 2000-06-22 Litmus Concepts, Inc. Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities
US5571684A (en) * 1994-11-07 1996-11-05 Litmus Concepts, Inc. Assay for proline iminopeptidase and other hydrolytic activities
KR970706902A (ko) * 1995-09-12 1997-12-01 로드릭 리차드 제이 Dna 증폭 및 검정 방법 및 장치(device and method for dna amplification and assay)
US20020086399A1 (en) * 1996-02-29 2002-07-04 Fuji Photo Film Co., Ltd. Method of measurement of protease and thin membranes used for said method
CN1225134A (zh) * 1996-05-14 1999-08-04 曼尼托巴大学 用固相法检测生物活性物质
CA2189486A1 (en) * 1996-11-04 1998-05-04 Yves St-Pierre Analysis of enzyme activity using immobilized fluorescence-labeled substrates
US6042878A (en) 1996-12-31 2000-03-28 General Electric Company Method for depositing a ceramic coating
US6451607B1 (en) 1998-05-07 2002-09-17 Litmus Concepts, Inc. External dried-reagent control for analytical test devices
US7700305B2 (en) 1999-09-17 2010-04-20 N2Itive1 Innovations Analyte detection
US7166475B2 (en) * 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
US6426189B1 (en) 1999-10-01 2002-07-30 Novozymes A/S Cellulose films for screening
EP1222306B1 (de) * 1999-10-01 2004-12-22 Novozymes A/S Zellulosefilme zur durchfuehrung von screening-verfahren
US6627396B1 (en) * 1999-10-28 2003-09-30 The Regents Of The University Of California Influenza sensor
US8182986B1 (en) * 2001-07-12 2012-05-22 Discoverx Assays for nucleic acid modifying enzymes
US6942972B2 (en) 2001-10-24 2005-09-13 Beckman Coulter, Inc. Efficient synthesis of protein-oligonucleotide conjugates
WO2003097237A2 (en) * 2002-05-13 2003-11-27 Becton, Dickinson, And Company Protease inhibitor sample collection system
US7041469B2 (en) * 2002-10-10 2006-05-09 Quidel Corporation Assays for trichomonal and other hydrolases
JP2006502415A (ja) * 2002-10-10 2006-01-19 ベクトン・ディキンソン・アンド・カンパニー カスパーゼ阻害剤を有するサンプル収集システム
KR20060036901A (ko) * 2003-05-02 2006-05-02 시그마-알드리치컴퍼니 고상 세포 용해 및 포획 플랫폼
US20050124965A1 (en) * 2003-12-08 2005-06-09 Becton, Dickinson And Company Phosphatase inhibitor sample collection system
GB2422664A (en) * 2005-01-28 2006-08-02 Ethicon Inc Device for detecting an enzyme in a sample
US20050191704A1 (en) * 2004-03-01 2005-09-01 Kimberly-Clark Worldwide, Inc. Assay devices utilizing chemichromic dyes
US7906276B2 (en) * 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US7094528B2 (en) 2004-06-30 2006-08-22 Kimberly-Clark Worldwide, Inc. Magnetic enzyme detection techniques
US7521226B2 (en) 2004-06-30 2009-04-21 Kimberly-Clark Worldwide, Inc. One-step enzymatic and amine detection technique
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
US7514230B2 (en) * 2004-10-22 2009-04-07 Kimberly-Clark Worldwide, Inc. Detection of trichomonas
KR100589354B1 (ko) 2005-01-25 2006-06-14 한국과학기술원 내부 다당류분해효소와 내부 단백질분해효소를 생산하는미생물을 동시에 탐지 및 선별하기 위한 배지 조성
US7504235B2 (en) 2005-08-31 2009-03-17 Kimberly-Clark Worldwide, Inc. Enzyme detection technique
US7745158B2 (en) * 2005-12-14 2010-06-29 Kimberly-Clark Worldwide, Inc. Detection of secreted aspartyl proteases from Candida species
US20070134747A1 (en) * 2005-12-14 2007-06-14 Kimberly-Clark Worldwide, Inc. Detection of secreted lipase proteins from Candida species
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Also Published As

Publication number Publication date
US5416003A (en) 1995-05-16
CN1138877A (zh) 1996-12-25
CN1096501C (zh) 2002-12-18
DE69424663D1 (de) 2000-06-29
US6251621B1 (en) 2001-06-26
DE69424663T2 (de) 2000-09-28
RU2159818C2 (ru) 2000-11-27
EP0694077B1 (de) 2000-05-24
US5585273A (en) 1996-12-17
EP0694077A1 (de) 1996-01-31

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