MX9703163A - Ensayo para la iminopeptidasa de prolina y otras actividades hidroliticas. - Google Patents
Ensayo para la iminopeptidasa de prolina y otras actividades hidroliticas.Info
- Publication number
- MX9703163A MX9703163A MX9703163A MX9703163A MX9703163A MX 9703163 A MX9703163 A MX 9703163A MX 9703163 A MX9703163 A MX 9703163A MX 9703163 A MX9703163 A MX 9703163A MX 9703163 A MX9703163 A MX 9703163A
- Authority
- MX
- Mexico
- Prior art keywords
- hydrolase
- action
- reporter group
- conjugate
- indicator
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/70—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases the product, e.g. phenol, naphthol being diazotised in situ, e.g. with Fast Red
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/30—Naphthyl amides, e.g. beta-NA, 2-NA, 4-methoxy-beta-naphthylamine, i.e. 4MNA
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se detecta la presencia de una hidrolasa activa enzimáticamente en una muestra de fluido por el contacto de la muestra con un conjugado de fase solida, el cual es susceptible al desdoblamiento pro la hidrolasa, y simultáneamente o brevemente después, el contacto de la muestra con un indicador, el cual sufre un cambio que se puede detectar en la accion de un grupo informador o marcador. Este grupo informador es parte del conjugado y se libera del mismo, parcial o totalmente, por la accion del la hidrolasa. Este indicador es susceptible a la accion por el grupo informador solamente al desacoplar este grupo informador desde el resto del conjugado, este desacoplamiento ocurre, en parte o totalmente, por la accion de la hidrolasa. También se suministran por esta invencion varias formas de dispositivos de prueba, secos, autocontenidos, que contienen el conjugado, descrito anteriormente, más el indicador y todos los demás reactivos y componentes necesarios para lograr una indicacion, que se puede detectar, de la presencia o ausencia de una hidrolasa activa catalíticamente. Las modalidades preferidas del dispositivo también contienen controles positivos y negativos.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33500794A | 1994-11-07 | 1994-11-07 | |
US08/374,487 US5571684A (en) | 1994-11-07 | 1995-01-17 | Assay for proline iminopeptidase and other hydrolytic activities |
PCT/US1995/013790 WO1996015255A2 (en) | 1994-11-07 | 1995-10-25 | Assay for proline iminopeptidase and other hydrolytic activities |
Publications (1)
Publication Number | Publication Date |
---|---|
MX9703163A true MX9703163A (es) | 1997-07-31 |
Family
ID=26989507
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX9703163A MX9703163A (es) | 1994-11-07 | 1995-10-25 | Ensayo para la iminopeptidasa de prolina y otras actividades hidroliticas. |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0791071B1 (es) |
AR (1) | AR001528A1 (es) |
AT (1) | ATE184654T1 (es) |
AU (1) | AU4135096A (es) |
CA (1) | CA2202993C (es) |
DE (1) | DE69512263T2 (es) |
MX (1) | MX9703163A (es) |
WO (1) | WO1996015255A2 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPQ826500A0 (en) * | 2000-06-21 | 2000-07-13 | Novapharm Research (Australia) Pty Limited | Enzyme detection and measurement |
US7041469B2 (en) * | 2002-10-10 | 2006-05-09 | Quidel Corporation | Assays for trichomonal and other hydrolases |
US7514230B2 (en) * | 2004-10-22 | 2009-04-07 | Kimberly-Clark Worldwide, Inc. | Detection of trichomonas |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1298763C (en) * | 1986-06-16 | 1992-04-14 | Mary Lou Gantzer | Rapid detection of n. gonorrhoeae without culture |
DE3813503A1 (de) * | 1988-04-22 | 1989-11-02 | Boehringer Mannheim Gmbh | Traegergebundenes mehrkomponentiges nachweissystem zur kolorimetrischen bestimmung esterolytisch oder/und proteolytisch aktiver inhaltsstoffe von koerperfluessigkeiten |
EP0475045B1 (en) * | 1990-08-06 | 1996-12-11 | Bayer Corporation | Method and device for the assay of ions |
DE4217474A1 (de) * | 1992-05-27 | 1993-12-02 | Boehringer Mannheim Gmbh | Verfahren und Mittel zur Bestimmung eines Analyts |
CZ289902B6 (cs) * | 1993-04-14 | 2002-04-17 | Litmus Concepts, Inc. | Způsob zjią»ování přítomnosti enzymaticky aktivní hydrolázy ve vzorku a testovací zařízení pro provádění tohoto způsobu |
-
1995
- 1995-10-25 AT AT95939592T patent/ATE184654T1/de not_active IP Right Cessation
- 1995-10-25 EP EP95939592A patent/EP0791071B1/en not_active Expired - Lifetime
- 1995-10-25 AU AU41350/96A patent/AU4135096A/en not_active Abandoned
- 1995-10-25 WO PCT/US1995/013790 patent/WO1996015255A2/en active IP Right Grant
- 1995-10-25 CA CA002202993A patent/CA2202993C/en not_active Expired - Fee Related
- 1995-10-25 DE DE69512263T patent/DE69512263T2/de not_active Expired - Fee Related
- 1995-10-25 MX MX9703163A patent/MX9703163A/es not_active IP Right Cessation
- 1995-11-06 AR AR33412895A patent/AR001528A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
EP0791071B1 (en) | 1999-09-15 |
EP0791071A1 (en) | 1997-08-27 |
CA2202993A1 (en) | 1996-05-23 |
ATE184654T1 (de) | 1999-10-15 |
WO1996015255A2 (en) | 1996-05-23 |
AU4135096A (en) | 1996-06-06 |
CA2202993C (en) | 2002-01-01 |
DE69512263T2 (de) | 2000-05-31 |
AR001528A1 (es) | 1997-11-26 |
DE69512263D1 (de) | 1999-10-21 |
WO1996015255A3 (en) | 1996-08-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant or registration | ||
MM | Annulment or lapse due to non-payment of fees |