WO2002010439A3 - Macromolecular enzyme substrates - Google Patents

Macromolecular enzyme substrates Download PDF

Info

Publication number
WO2002010439A3
WO2002010439A3 PCT/US2001/041496 US0141496W WO0210439A3 WO 2002010439 A3 WO2002010439 A3 WO 2002010439A3 US 0141496 W US0141496 W US 0141496W WO 0210439 A3 WO0210439 A3 WO 0210439A3
Authority
WO
WIPO (PCT)
Prior art keywords
activity
proteinase
endosaccharidase
sample
measuring
Prior art date
Application number
PCT/US2001/041496
Other languages
French (fr)
Other versions
WO2002010439A2 (en
Inventor
Glen L Hortin
Original Assignee
Government Of United States Of
Glen L Hortin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Government Of United States Of, Glen L Hortin filed Critical Government Of United States Of
Priority to AU2001285392A priority Critical patent/AU2001285392A1/en
Publication of WO2002010439A2 publication Critical patent/WO2002010439A2/en
Publication of WO2002010439A3 publication Critical patent/WO2002010439A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention features methods for measuring the activity of an enzyme (such as a proteinase or an endosaccharidase) in a sample, using a macromolecular substrate of the enzyme. Also featured are methods for: detecting the level of peptidase activity of a proteinase; measuring amylase activity in a sample; diagnosing pancreatitis in a subject; measuring the activity of a target isoenzyme in a sample; identifying a compound that modulates the activity of a proteinase or an endosaccharidase; and identifying an antibody that modulates the activity of a proteinase or an endosaccharidase, using the macromolecular substrates provided by the present invention.
PCT/US2001/041496 2000-07-31 2001-07-31 Macromolecular enzyme substrates WO2002010439A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001285392A AU2001285392A1 (en) 2000-07-31 2001-07-31 Macromolecular enzyme substrates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22179000P 2000-07-31 2000-07-31
US60/221,790 2000-07-31

Publications (2)

Publication Number Publication Date
WO2002010439A2 WO2002010439A2 (en) 2002-02-07
WO2002010439A3 true WO2002010439A3 (en) 2003-02-13

Family

ID=22829402

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/041496 WO2002010439A2 (en) 2000-07-31 2001-07-31 Macromolecular enzyme substrates

Country Status (2)

Country Link
AU (1) AU2001285392A1 (en)
WO (1) WO2002010439A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017138A2 (en) * 2003-08-19 2005-02-24 Novozymes A/S Active-site titration of glycosyl hydrolases
DE102006035899A1 (en) * 2006-07-31 2008-02-07 Dade Behring Marburg Gmbh New polysaccharide-peptide-conjugate, where the peptide portion contains a sequence having a signal group that is splittable by thrombin at its carbon-terminal end, useful in a procedure for determining the thrombin generation in a sample
EP2712423A4 (en) * 2011-02-25 2015-06-10 Wellstat Diagnostics Llc Assays for detecting enzymatic activity
RU2019143354A (en) * 2017-05-30 2021-06-30 Юджин Й. ЧАНЬ FLUOROGENIC PEPTIDE SUBSTRATES FOR MEASURING FACTOR XA IN SOLUTION AND SOLID PHASE

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0310940A2 (en) * 1987-09-30 1989-04-12 Fujirebio Kabushiki Kaisha Analytical element for enzyme immunoassays
EP0347839A2 (en) * 1988-06-24 1989-12-27 Fujirebio Kabushiki Kaisha Dry-type analytical element for immunoassay
US5302514A (en) * 1991-06-26 1994-04-12 Kikkoman Corporation Maltooligoside derivative, reagent for determining α-amylase activity containing the same as effective ingredient and a process for determining the α-amylase activity using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0310940A2 (en) * 1987-09-30 1989-04-12 Fujirebio Kabushiki Kaisha Analytical element for enzyme immunoassays
EP0347839A2 (en) * 1988-06-24 1989-12-27 Fujirebio Kabushiki Kaisha Dry-type analytical element for immunoassay
US5302514A (en) * 1991-06-26 1994-04-12 Kikkoman Corporation Maltooligoside derivative, reagent for determining α-amylase activity containing the same as effective ingredient and a process for determining the α-amylase activity using same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HORTIN GLEN L ET AL: "Macromolecular chromogenic substrates for measuring proteinase activity.", CLINICAL CHEMISTRY, vol. 47, no. 2, February 2001 (2001-02-01), pages 215 - 222, XP002202570, ISSN: 0009-9147 *
JULIANO M A ET AL: "SYNTHESIS AND HYDROLYSIS BY ARGINYLHYDROLASES OF P NITROANILIDE CHROMOGENIC SUBSTRATES CONTAINING POLYETHYLENE GLYCOL AND D GLUCONYL MOIETIES", PEPTIDE RESEARCH, vol. 4, no. 6, 1991, pages 334 - 339, XP002202569, ISSN: 1040-5704 *

Also Published As

Publication number Publication date
WO2002010439A2 (en) 2002-02-07
AU2001285392A1 (en) 2002-02-13

Similar Documents

Publication Publication Date Title
WO2002079486A3 (en) Milieux de detection specific de staphylococcus aureus
WO2002066671A3 (en) Improved method for detection of atp
WO2006052419A3 (en) Nucleic acid enzyme light-up sensor utilizing invasive dna
WO2005074604A3 (en) METHODS OF DETECTING Lp-PLA2 ACTIVITY
WO2006094116A3 (en) Enzyme sensors including environmentally sensitive or fluorescent labels and uses thereof
WO2001075145A3 (en) Ubiquitin ligase assay
WO2003023363A3 (en) Microcantilever apparatus and methods for detection of enzymes
WO2004000884A3 (en) Compositions and methods for apo-b48 and apo-b100 assay
WO2003046531A3 (en) Method for detecting defects in substrates
AU4299800A (en) Method for assessing the risk of peptic ulcer, comprising the steps of determining quantitatively the concentrations of pepsinogen i (pgi) and gastrin-17 in a serum sample
WO2005012329A3 (en) Kinase and phosphatase assays
WO2004048935A3 (en) Detection of protease enzymes
AU3946599A (en) Nucleic acid sequence identification
WO2000075363A3 (en) Enzymatic measurement of mycophenolic acid
ATE503021T1 (en) METHOD FOR DETECTING ENDOGLYCOSIDASE ACTIVITY
WO2002038540A3 (en) Activity-dependent cysteine protease profiling reagent
WO2001040504A3 (en) Methods and compositions for detecting nitroaromatic compounds
DE60026598D1 (en) NEW ENZYMATIC SUBSTRATES FOR THE DETECTION OF PSEUDOMONAS AERUGINOSA
WO2002010439A3 (en) Macromolecular enzyme substrates
MX2008002123A (en) Methods and compositions for determining a level of biologically active serum paraoxonase.
FR2875240B1 (en) METHOD OF DETECTING STREPTOCOCCUS AGALACTIAE USING ALPHA-GLUCOSIDASE ACTIVITY
WO2008064016A3 (en) Detecting molecular interactions
WO2004042352A3 (en) Substrates of n-end rule ubiquitylation and methods for measuring the ubiquitylation of these substrates
CA2160262A1 (en) Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities
WO2004101812A8 (en) Chromogenic plating medium for the rapid presumptive identification of bacillus anthracis, bacillus cereus, and bacillus thuringiensis

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 10343387

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP