ATE173012T1 - Verwendung eines mittels zur verformung von hepatozyten-sphäroiden und verfahren zur züchtung von hepatozyten für sphäroid-bildung - Google Patents

Verwendung eines mittels zur verformung von hepatozyten-sphäroiden und verfahren zur züchtung von hepatozyten für sphäroid-bildung

Info

Publication number
ATE173012T1
ATE173012T1 AT92114764T AT92114764T ATE173012T1 AT E173012 T1 ATE173012 T1 AT E173012T1 AT 92114764 T AT92114764 T AT 92114764T AT 92114764 T AT92114764 T AT 92114764T AT E173012 T1 ATE173012 T1 AT E173012T1
Authority
AT
Austria
Prior art keywords
spheroids
hepatocytes
agent
deforming
spheroid formation
Prior art date
Application number
AT92114764T
Other languages
German (de)
English (en)
Inventor
Toshikazu Yada
Norio Koide
Koji Kimata
Original Assignee
Seikagaku Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Kogyo Co Ltd filed Critical Seikagaku Kogyo Co Ltd
Application granted granted Critical
Publication of ATE173012T1 publication Critical patent/ATE173012T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/905—Hyaluronic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
AT92114764T 1991-08-30 1992-08-28 Verwendung eines mittels zur verformung von hepatozyten-sphäroiden und verfahren zur züchtung von hepatozyten für sphäroid-bildung ATE173012T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP24415291A JP3199405B2 (ja) 1991-08-30 1991-08-30 肝細胞球状集塊化剤及び球状集塊化肝細胞の製造方法

Publications (1)

Publication Number Publication Date
ATE173012T1 true ATE173012T1 (de) 1998-11-15

Family

ID=17114538

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92114764T ATE173012T1 (de) 1991-08-30 1992-08-28 Verwendung eines mittels zur verformung von hepatozyten-sphäroiden und verfahren zur züchtung von hepatozyten für sphäroid-bildung

Country Status (10)

Country Link
US (1) US5624839A (enExample)
EP (1) EP0529659B1 (enExample)
JP (1) JP3199405B2 (enExample)
KR (1) KR100191363B1 (enExample)
AT (1) ATE173012T1 (enExample)
AU (1) AU654376B2 (enExample)
CA (1) CA2077175A1 (enExample)
DE (1) DE69227493T2 (enExample)
FI (1) FI923874A7 (enExample)
NO (1) NO923376L (enExample)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6457314A (en) * 1987-08-27 1989-03-03 Fanuc Ltd Numerical controller
US5610148A (en) * 1991-01-18 1997-03-11 University College London Macroscopically oriented cell adhesion protein for wound treatment
US5629287A (en) * 1991-01-18 1997-05-13 University College London Depot formulations
US5856112A (en) * 1994-06-16 1999-01-05 Urocor, Inc. Method for selectively inducing biomarker expression in urologic tumor tissue for diagnosis and treatment thereof
AU5875598A (en) * 1997-02-05 1998-08-26 University Of Hertfordshire Preparation of spheroids and their use in medicin or diagnosis
IT1293484B1 (it) * 1997-06-11 1999-03-01 Fidia Advanced Biopolymers Srl Materiale biologico comprendente una efficiente coltura di cellule e una matrice tridimensionale biocompatibile e biodegradabile
GB9913979D0 (en) * 1999-06-17 1999-08-18 Univ Wales Medicine Spheroid preparation
FR2795724B1 (fr) * 1999-07-02 2002-12-13 Sanofi Synthelabo Nouveaux derives du benzene, un procede pour leur preparation et les compositions pharmaceutiques les contenant
US8076312B2 (en) 2000-01-10 2011-12-13 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Use of lipid conjugates in the treatment of disease
US7772196B2 (en) 2000-01-10 2010-08-10 Yissum Research Development Company Of The Hebrew University Of Jerusalem Use of lipid conjugates in the treatment of diseases
US7608598B2 (en) 2000-01-10 2009-10-27 Yissum Research Development Company Of The Hebrew University Of Jerusalem Use of lipid conjugates in the treatment of conjunctivitis
WO2001051003A2 (en) * 2000-01-10 2001-07-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Use of lipid conjugates in the treatment of disease
US8916539B2 (en) 2000-01-10 2014-12-23 Yissum Research Development Company Of The Hebrew University Of Jerusalem Use of lipid conjugates in the treatment of disease
US7893226B2 (en) 2004-09-29 2011-02-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Use of lipid conjugates in the treatment of diseases
US9040078B2 (en) 2000-01-10 2015-05-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Use of lipid conjugates in the treatment of diseases of the nervous system
US8304395B2 (en) 2000-01-10 2012-11-06 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Lipid conjugates in the treatment of disease
US8883761B2 (en) 2001-01-10 2014-11-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Use of lipid conjugates in the treatment of diseases associated with vasculature
KR20040037062A (ko) * 2001-08-14 2004-05-04 텔 아비브 유니버시티 퓨쳐 테크날러지 디벨로프멘트 엘피 지질화된 글리코스아미노글리칸 입자 및 그의 진단 및치료용 약물 및 유전자 송달 용도
WO2003072764A1 (en) * 2002-02-26 2003-09-04 Stelsys Llc Hepatocyte bioreactor system for long term culture of functional hepatocyte spheroids
US7160719B2 (en) 2002-06-07 2007-01-09 Mayo Foundation For Medical Education And Research Bioartificial liver system
EP1601757B1 (en) 2003-02-24 2016-11-30 Marine Polymer Technologies, Inc. Cell-polymer fiber compositions and use thereof
US8137685B2 (en) 2003-10-29 2012-03-20 Teijin Limited Hyaluronic acid compound, hydrogel thereof and joint treating material
US8906882B2 (en) 2005-11-17 2014-12-09 Yissum Research Development Company Of The Hebrew University Of Jerusalem Lipid conjugates in the treatment of allergic rhinitis
US8859524B2 (en) 2005-11-17 2014-10-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Lipid conjugates in the treatment of chronic rhinosinusitis
CA2705785A1 (en) 2006-11-14 2008-05-22 Saul Yedgar Use of lipid conjugates in the treatment of diseases or disorders of the eye
JP4964577B2 (ja) * 2006-12-15 2012-07-04 有限会社応用糖質化学研究所 親脂質性ヘパリン修飾体、その製造方法及び毛髪成長促進剤
JP4942681B2 (ja) * 2008-02-18 2012-05-30 生化学工業株式会社 角膜障害症治癒促進剤
JP5501589B2 (ja) * 2008-08-28 2014-05-21 電気化学工業株式会社 ヒアルロン酸及び／又はその塩の精製法
CN102421467B (zh) 2009-03-13 2015-04-22 梅约医学教育与研究基金会 生物人工肝
GB0913442D0 (en) 2009-07-31 2009-09-16 Univ Ramot Cell-targeting nanoparticles comprising polynucleotide agents and uses thereof
TWI499670B (zh) * 2011-12-20 2015-09-11 Univ Chang Gung Preparation of Cell Sphere without Culture in High Molecular Weight Hyaluronic Acid Nanometer Coatings
IN2014MN01886A (enExample) 2012-04-18 2015-07-10 Univ Ramot
US9908972B2 (en) * 2013-05-22 2018-03-06 National Institute Of Advanced Industrial Science And Technology Photodegradable cross-linking agent, photodegradable gel, cell culture instrument, cell arrangement-sorting apparatus, cell arrangement method, cell sorting method, tissue forming method, and tissue
EP3160448B1 (en) 2014-06-26 2025-08-06 Ramot at Tel-Aviv University Ltd. Liposomal formulations for delivery of nucleic acids

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2374910A1 (fr) * 1976-10-23 1978-07-21 Choay Sa Preparation a base d'heparine, comprenant des liposomes, procede pour l'obtenir et medicaments contenant de telles preparations
US4391909A (en) * 1979-03-28 1983-07-05 Damon Corporation Microcapsules containing viable tissue cells
US4352883A (en) * 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
FR2461724A1 (fr) * 1979-07-20 1981-02-06 Christine Fougnot Polymeres substitues par des groupes leur conferant des proprietes anti-coagulantes et leur procede de preparation, objets constitues par et/ou comprenant lesdits polymeres et leurs procedes de fabrication, application desdits objets en chirurgie et en medecine, et compositions pharmaceutiques contenant lesdits polymeres substitues
US4352887A (en) * 1979-10-29 1982-10-05 Albert Einstein College Of Medicine Of Yeshiva University Method and article for culturing differentiated cells
US4385046A (en) * 1980-12-15 1983-05-24 Minnesota Mining And Manufacturing Company Diagnostic radio-labeled polysaccharide derivatives
US4956128A (en) * 1984-05-25 1990-09-11 Connaught Laboratories Limited Droplet generation
US4859581A (en) * 1986-03-10 1989-08-22 Board Of Regents, The University Of Texas System Endoglycosidase assay
US4745105A (en) * 1986-08-20 1988-05-17 Griffin Charles C Low molecular weight heparin derivatives with improved permeability
US4942129A (en) * 1987-07-28 1990-07-17 Queen's University At Kingston Multiple membrane microencapsulation
JPH01277486A (ja) * 1988-04-30 1989-11-07 Tosoh Corp 肝細胞の球状培養方法
JPH0661455B2 (ja) * 1988-11-25 1994-08-17 日本油脂株式会社 多糖誘導体によって安定化された脂肪乳剤
CA2067211C (en) * 1990-07-24 2004-06-15 Katsukiyo Sakurai Phospholipid- or lipid-linked glycosaminoglycan, process for producing the same and metastasis inhibitor
JP3041031B2 (ja) * 1990-10-09 2000-05-15 財団法人神奈川科学技術アカデミー 肝細胞増殖剤及びそれを用いた肝細胞の増殖方法並びにハイブリッド型人工肝臓用素材
US5270192A (en) * 1991-02-07 1993-12-14 Monsanto Company Biological artificial liver

Also Published As

Publication number Publication date
EP0529659A2 (en) 1993-03-03
KR100191363B1 (ko) 1999-06-15
DE69227493D1 (de) 1998-12-10
AU654376B2 (en) 1994-11-03
EP0529659B1 (en) 1998-11-04
AU2138592A (en) 1993-03-11
JPH05236951A (ja) 1993-09-17
FI923874L (fi) 1993-03-01
DE69227493T2 (de) 1999-05-06
NO923376L (no) 1993-03-01
CA2077175A1 (en) 1993-03-01
FI923874A7 (fi) 1993-03-01
EP0529659A3 (enExample) 1994-04-27
NO923376D0 (no) 1992-08-28
FI923874A0 (fi) 1992-08-28
US5624839A (en) 1997-04-29
JP3199405B2 (ja) 2001-08-20

Similar Documents

Publication Publication Date Title
DE69227493D1 (de) Verwendung eines Mittels zur Verformung von Hepatozyten-Sphäroiden und Verfahren zur Züchtung von Hepatozyten für Sphäroid-Bildung
ATE406435T1 (de) Methoden zur züchtung von tierzellen
DE60029701D1 (de) Verfahren zur Herstellung von L-Glutaminsäure durch Fermentation und in Verbindung mit Fällung
ATE38855T1 (de) Verfahren und einrichtung zur zellenkultur.
ATE153700T1 (de) Verfahren zur herstellung einer zeaxanthin enthaltenden zusammensetzung mittels eines mikroorganismus der spezies flavobacterium multivorum
ATE161041T1 (de) Verfahren zur hcv-züchtung in zell-linien aus b- oder t-lymphozyten
DE69334254D1 (de) Verfahren zur Zellkultivierung in einer Monoschicht
DK1130394T3 (da) In vitro-modeller af CNS-funktion og -dysfunktion
ATE220714T1 (de) Verfahren zur herstellung von proteinen
ATE180830T1 (de) Verfahren zur kultivierung von vorläufer t- zellinien
Robinson et al. Morphologic plasticity and periodicity: porcine cerebral microvascular cells in culture
DE3784677D1 (de) Verfahren zur herstellung von l-sorbose und vorrichtung zur kultivierung von mikroorganismen.
DE60021373D1 (de) Verfahren der zellkultivierung
ATE140487T1 (de) Verfahren und vorrichtung zur forschung und quantifizierung der wechselwirkungen von substanzen auf biologische zellen
ATE186352T1 (de) Verfahren zur befestigung von hängen
ATE95244T1 (de) Verfahren zur herstellung von l-phosphinothricin.
DE3761611D1 (de) Verfahren zur abklaerung und stabilisierung eines zellkulturmediums.
JP2610997B2 (ja) 肝細胞培養方法
ATE125293T1 (de) Verfahren zur kultur von tomaten-protoplasten.
DE50202362D1 (de) Verfahren zur Erhöhung der Wachstumsrate von Zellen in statischen Flüssigmedien
ATE313622T1 (de) Verfahren zur züchtung von haftenden tierischen zellen
ATE127527T1 (de) Verfahren zur herstellung eines metastaseninhibitorenfaktors.
JPS57186491A (en) Cultivation of fibroblast using fibronectin
DE3871322D1 (de) Verfahren zur kultur von pflanzengewebe.
OA05397A (fr) Milieu et procédé pour la culture de micro-organismes.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties